##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937447_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2215822 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.650048153687436 33.0 31.0 34.0 30.0 34.0 2 31.77460689531921 33.0 31.0 34.0 30.0 34.0 3 31.814334815702704 33.0 31.0 34.0 30.0 34.0 4 35.41916318188013 37.0 35.0 37.0 33.0 37.0 5 35.41771947385666 37.0 35.0 37.0 33.0 37.0 6 35.44638919552202 37.0 35.0 37.0 33.0 37.0 7 35.37493038700762 37.0 35.0 37.0 33.0 37.0 8 35.35847509411857 37.0 35.0 37.0 33.0 37.0 9 36.95279945771817 39.0 37.0 39.0 33.0 39.0 10-11 36.83315401688402 39.0 37.0 39.0 33.0 39.0 12-13 36.689775171471354 39.0 37.0 39.0 32.5 39.0 14-15 37.79731043378033 40.0 38.0 41.0 33.0 41.0 16-17 37.70502278612632 40.0 37.5 41.0 32.5 41.0 18-19 37.600484831362806 40.0 37.0 41.0 32.0 41.0 20-21 37.453610669088036 40.0 37.0 41.0 32.0 41.0 22-23 37.53565539109188 40.0 37.0 41.0 32.0 41.0 24-25 37.49705752537884 40.0 37.0 41.0 32.0 41.0 26-27 37.41543364042779 40.0 37.0 41.0 32.0 41.0 28-29 37.261309121400544 40.0 37.0 41.0 31.5 41.0 30-31 37.01551726627861 39.0 36.5 41.0 31.0 41.0 32-33 36.79157870081622 39.0 36.0 41.0 30.5 41.0 34-35 36.8003664102983 39.0 36.0 41.0 30.0 41.0 36-37 36.66674466631345 39.0 36.0 41.0 30.0 41.0 38-39 36.527132594585666 39.0 36.0 41.0 30.0 41.0 40-41 36.29448191235578 39.0 35.5 41.0 30.0 41.0 42-43 36.02501870637624 39.0 35.0 40.0 29.0 41.0 44-45 35.804771773183944 39.0 35.0 40.0 28.5 41.0 46-47 35.4287769956251 38.5 35.0 40.0 26.5 41.0 48-49 35.1049592431161 38.0 34.5 40.0 25.5 41.0 50-51 34.16306860388605 37.5 33.5 39.5 24.0 40.5 52-53 34.27511302803204 38.0 33.5 39.5 24.0 40.5 54-55 34.56157579444559 38.0 34.0 40.0 24.0 41.0 56-57 34.272180256356336 38.0 34.0 40.0 23.0 41.0 58-59 33.9709227546256 38.0 33.0 40.0 22.5 41.0 60-61 33.61456989776255 37.5 33.0 40.0 21.5 41.0 62-63 33.20118764052347 37.0 32.5 40.0 19.5 41.0 64-65 32.74048479525882 36.5 32.0 39.0 18.0 41.0 66-67 32.282512990664415 36.0 31.0 39.0 14.5 40.0 68-69 31.753547893287454 35.5 31.0 39.0 10.0 40.0 70-71 31.25652737449127 35.0 30.5 38.0 7.5 40.0 72-73 30.72830331136707 35.0 30.0 37.5 4.5 39.5 74-75 30.13735354193613 34.0 29.0 37.0 2.0 39.0 76-77 29.56451556126801 34.0 29.0 36.0 2.0 39.0 78-79 28.968530865746438 34.0 28.0 36.0 2.0 38.0 80-81 28.331024784481787 34.0 26.5 35.0 2.0 37.0 82-83 27.720471906136865 33.0 26.0 35.0 2.0 37.0 84-85 27.082097072779312 33.0 25.5 35.0 2.0 36.0 86-87 26.42225706758034 32.0 24.5 35.0 2.0 36.0 88-89 25.7924993523848 32.0 22.0 34.5 2.0 35.0 90-91 25.15529338547952 31.5 19.5 34.0 2.0 35.0 92-93 24.407536571078364 31.0 15.0 34.0 2.0 35.0 94-95 23.563612284741282 31.0 4.5 34.0 2.0 35.0 96-97 22.62832167926846 30.5 2.0 34.0 2.0 35.0 98-99 21.36942791433608 29.5 2.0 34.0 2.0 35.0 100-101 18.33544797370908 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1856289856234774 1101 2 0.2876712591866273 1101 3 0.23446658389736896 1101 4 0.1625911986937041 1101 5 0.188977043686954 1101 6 0.22252126163327546 1101 7 0.1554951310420165 1101 8 0.219109308607095 1101 9 0.15855989569254803 1101 10-11 0.23488903165308272 1101 12-13 0.40708732939932446 1101 14-15 0.42928222691875817 1101 16-17 0.450709786978571 1101 18-19 0.44594019723918166 1101 20-21 0.39631440433071674 1101 22-23 0.48283857705681044 1101 24-25 0.46621902643779833 1101 26-27 0.5301375105548729 1101 28-29 0.47499336874815157 1101 30-31 0.4870636880623138 1101 32-33 0.40182355004636605 1101 34-35 0.45104722863857205 1101 36-37 0.36991888912184834 1101 38-39 0.4529192872886938 1101 40-41 0.5704816015544765 1101 42-43 0.6646725970905294 1101 44-45 0.6532959274064822 1101 46-47 0.833132623564893 1101 48-49 0.8579495689808638 1101 50-51 0.8692238381725232 1101 52-53 0.8192118424592678 1101 54-55 1.0093621006557143 1101 56-57 1.0165926342600713 1101 58-59 0.9255865902778169 1101 60-61 0.9668908990635003 1101 62-63 0.89652481775898 1101 64-65 0.9522823686141351 1101 66-67 1.123242776960538 1101 68-69 1.1343625423776267 1101 70-71 1.0944416588507195 1101 72-73 1.2346602291625928 1101 74-75 1.2401594114278076 1101 76-77 1.2311585782105219 1101 78-79 1.3927571026236016 1101 80-81 1.555907641558555 1101 82-83 1.3619814726477095 1101 84-85 1.5142353786518683 1101 86-87 1.5696706875000785 1101 88-89 1.733784232943723 1101 90-91 1.8170899414694546 1101 92-93 2.1185438019049023 1101 94-95 2.3403388696910916 1101 96-97 2.1459817061842763 1101 98-99 2.560411925346326 1101 100-101 2.4086487240163876 1102 1 0.30963388035078054 1102 2 0.28994451466162374 1102 3 0.18619922236539566 1102 4 0.22706269380159938 1102 5 0.13749980094409153 1102 6 0.12963788567574142 1102 7 0.07321551489971512 1102 8 0.0943099658972315 1102 9 0.18331709342145075 1102 10-11 0.17598780674805425 1102 12-13 0.27665639736616754 1102 14-15 0.4258813986022858 1102 16-17 0.5074366454046313 1102 18-19 0.6032414537886908 1102 20-21 0.4312867956350317 1102 22-23 0.4503865445742008 1102 24-25 0.5916175808633639 1102 26-27 0.696938226749495 1102 28-29 0.646642584617986 1102 30-31 0.7130590395646408 1102 32-33 0.6948586511125043 1102 34-35 0.8027964200919584 1102 36-37 0.7516191348907881 1102 38-39 0.7271669882585456 1102 40-41 0.8540228824282892 1102 42-43 0.9553125987299467 1102 44-45 0.935255937490787 1102 46-47 0.9658991627693325 1102 48-49 0.9361565470650035 1102 50-51 0.8388641221022937 1102 52-53 0.8412671068725572 1102 54-55 0.9894642843630308 1102 56-57 1.0058344646390012 1102 58-59 1.1259689769482435 1102 60-61 1.0866805808347522 1102 62-63 1.0010237190188391 1102 64-65 1.00926101669274 1102 66-67 1.197696818359205 1102 68-69 1.2185960303378742 1102 70-71 1.2680244127368994 1102 72-73 1.326102129216558 1102 74-75 1.469559642121137 1102 76-77 1.4266212969001622 1102 78-79 1.5648678004749073 1102 80-81 1.6883822000807456 1102 82-83 1.613577239213761 1102 84-85 1.6585213341745337 1102 86-87 1.8458105440228678 1102 88-89 2.0200028241702874 1102 90-91 1.9794153150196578 1102 92-93 2.012137018167433 1102 94-95 2.11359386811284 1102 96-97 2.123573936257433 1102 98-99 2.300638793383289 1102 100-101 2.174013015986265 1103 1 0.3219594599588902 1103 2 0.3966803588687 1103 3 0.3526585898110177 1103 4 0.29317785288802867 1103 5 0.3044211299931092 1103 6 0.33318858574823906 1103 7 0.29011511211359675 1103 8 0.3196456138773698 1103 9 0.4613334422511386 1103 10-11 0.4153183296014902 1103 12-13 0.3761572909758115 1103 14-15 0.544730542094733 1103 16-17 0.5034081865204953 1103 18-19 0.48249843850297225 1103 20-21 0.4250526195277544 1103 22-23 0.42751235781020824 1103 24-25 0.5552942487283445 1103 26-27 0.31685116278445946 1103 28-29 0.36026387686378314 1103 30-31 0.4650777093030811 1103 32-33 0.4477496683075586 1103 34-35 0.5878617394133059 1103 36-37 0.5804123221099076 1103 38-39 0.7431144834617385 1103 40-41 0.9418471628227607 1103 42-43 0.69175402567042 1103 44-45 0.8374737801386303 1103 46-47 0.6881286526561325 1103 48-49 0.72400815236022 1103 50-51 0.7823324888331982 1103 52-53 0.6863879469766303 1103 54-55 0.9978387687122634 1103 56-57 0.9667007664613081 1103 58-59 1.0991197856372281 1103 60-61 0.9919834901681668 1103 62-63 1.1138727412570333 1103 64-65 1.0761011395523212 1103 66-67 1.4006497862276888 1103 68-69 1.4916166187966944 1103 70-71 1.4614771957353412 1103 72-73 1.421976410763797 1103 74-75 1.3317546172426695 1103 76-77 1.2880241060707434 1103 78-79 1.1379922235565942 1103 80-81 1.1334282382690013 1103 82-83 1.4451601280193493 1103 84-85 1.6247122344702412 1103 86-87 1.8007249701188321 1103 88-89 1.776233712750443 1103 90-91 1.7017640097860003 1103 92-93 1.803071128438777 1103 94-95 1.7993282205511143 1103 96-97 1.9153564846972237 1103 98-99 1.7479915655371663 1103 100-101 1.6785878874951905 1104 1 0.28088010540053787 1104 2 0.34199857350527196 1104 3 0.2919692868075181 1104 4 0.2793473227472987 1104 5 0.22642316242751548 1104 6 0.34638013339123574 1104 7 0.4003479628142159 1104 8 0.3925765297071919 1104 9 0.4713933162131454 1104 10-11 0.5074831427849276 1104 12-13 0.4548534217414897 1104 14-15 0.7210561475555934 1104 16-17 0.6431885246621221 1104 18-19 0.5702581783784169 1104 20-21 0.6000163924835249 1104 22-23 0.5800090225549113 1104 24-25 0.5384315530304349 1104 26-27 0.5381255262114593 1104 28-29 0.6404769781185777 1104 30-31 0.5392410209875464 1104 32-33 0.7019456646484983 1104 34-35 0.7890177921619141 1104 36-37 0.884819339926679 1104 38-39 0.7826708649168808 1104 40-41 0.8003669012778758 1104 42-43 0.8405048055875284 1104 44-45 0.8171283001070506 1104 46-47 0.9375715931592339 1104 48-49 0.8471234583498202 1104 50-51 0.7719351157800318 1104 52-53 0.8120982422206993 1104 54-55 0.9643942320210712 1104 56-57 1.0716557975819043 1104 58-59 1.2532059595033616 1104 60-61 1.2139399534617539 1104 62-63 1.2928548839028196 1104 64-65 1.1830305234473926 1104 66-67 1.3501392258540648 1104 68-69 1.1862091089346443 1104 70-71 1.1203315587903546 1104 72-73 1.2224455446457654 1104 74-75 1.1348063124316177 1104 76-77 1.1619249936121925 1104 78-79 1.2513389091836302 1104 80-81 1.2961305305912347 1104 82-83 1.454025904495527 1104 84-85 1.4873175549844717 1104 86-87 1.4440673101914356 1104 88-89 1.5701130754250059 1104 90-91 1.6048687139754882 1104 92-93 1.6418338121314484 1104 94-95 1.6862396473302148 1104 96-97 1.7687092405734433 1104 98-99 2.02150684853083 1104 100-101 1.9863794496797347 1105 1 0.28032075031345016 1105 2 0.15199098127006394 1105 3 0.2473720485867723 1105 4 0.2790331559928063 1105 5 0.2084837030524298 1105 6 0.1997876213445977 1105 7 0.2950265156868639 1105 8 0.3144444100663435 1105 9 0.3433538488801915 1105 10-11 0.3863578020791394 1105 12-13 0.3878951919398048 1105 14-15 0.5297199141584201 1105 16-17 0.46538727100618615 1105 18-19 0.5128897927408005 1105 20-21 0.4696134540334853 1105 22-23 0.6098559396188818 1105 24-25 0.6282490628571793 1105 26-27 0.6323252397897008 1105 28-29 0.4997821211435749 1105 30-31 0.6656665622377176 1105 32-33 0.6584986300907687 1105 34-35 0.7153655829013559 1105 36-37 0.7349852261181198 1105 38-39 0.7557122848461404 1105 40-41 0.7431834822771322 1105 42-43 0.7252808069959826 1105 44-45 0.6891791653176753 1105 46-47 0.7807129718857979 1105 48-49 0.7778615950598251 1105 50-51 0.8057873901168975 1105 52-53 0.6448879416068962 1105 54-55 0.9766081751257047 1105 56-57 1.0838758089441498 1105 58-59 0.9113916856710063 1105 60-61 1.0269307718039329 1105 62-63 0.831304209035757 1105 64-65 1.0420967000241674 1105 66-67 1.1672066074792653 1105 68-69 1.0727284669836585 1105 70-71 1.1768359474464347 1105 72-73 1.2763781442781053 1105 74-75 1.505160984421554 1105 76-77 1.2662511696789203 1105 78-79 1.4259221427875808 1105 80-81 1.497995037306417 1105 82-83 1.4468956249961877 1105 84-85 1.5151245389191033 1105 86-87 1.5466939132484256 1105 88-89 1.757139693721772 1105 90-91 1.609489730548436 1105 92-93 1.8361898102277046 1105 94-95 1.7908253758329913 1105 96-97 1.5873340904416757 1105 98-99 1.563660192017867 1105 100-101 1.3949770492306577 1106 1 0.12179090120824299 1106 2 -0.4034752338242029 1106 3 0.009179076182252999 1106 4 0.15725034755753597 1106 5 0.140154144457469 1106 6 0.03378502440437359 1106 7 0.1767499879275789 1106 8 0.2632864828913739 1106 9 0.16836536756664344 1106 10-11 -0.2028881764781474 1106 12-13 -0.03633550565876931 1106 14-15 -0.023839733938224583 1106 16-17 0.07296460327840038 1106 18-19 0.21696005897205595 1106 20-21 0.08736173034432682 1106 22-23 0.13719440126090632 1106 24-25 0.2828809772479062 1106 26-27 0.18725469531486283 1106 28-29 0.23054423184563433 1106 30-31 0.3956235803340249 1106 32-33 0.33923673106327357 1106 34-35 0.3617949209957416 1106 36-37 0.3861145061703013 1106 38-39 0.5458575250107529 1106 40-41 0.4558310560977006 1106 42-43 0.35119547671634166 1106 44-45 0.45171555389533324 1106 46-47 0.551090388379059 1106 48-49 0.7055603335392746 1106 50-51 0.6207242654712388 1106 52-53 0.5230170600982831 1106 54-55 0.6838916126009309 1106 56-57 0.46238667091587615 1106 58-59 0.3778230357957 1106 60-61 0.4818937362351221 1106 62-63 0.6952952618586536 1106 64-65 0.7828161167896894 1106 66-67 1.0440695119235315 1106 68-69 1.0583918161689034 1106 70-71 1.2352944031346453 1106 72-73 1.1852586962475726 1106 74-75 0.8328387237372255 1106 76-77 1.1066837038348147 1106 78-79 1.2713049072424099 1106 80-81 1.23394111788636 1106 82-83 1.050886083185599 1106 84-85 1.0094821589789724 1106 86-87 1.1816403737954317 1106 88-89 1.382489835611043 1106 90-91 1.2160518850813382 1106 92-93 1.6694148442227892 1106 94-95 1.4882969867586873 1106 96-97 1.2611072194404933 1106 98-99 1.5175803974394277 1106 100-101 1.0734345998792172 1107 1 0.15841681283415454 1107 2 0.20377487342590328 1107 3 0.22496629196946927 1107 4 0.1539255942327813 1107 5 0.05151193081525207 1107 6 0.12998837060771962 1107 7 0.29853248471007277 1107 8 0.18392140352629127 1107 9 0.30548462968590684 1107 10-11 0.18913777804780096 1107 12-13 0.20586204903877814 1107 14-15 0.2680950579965611 1107 16-17 0.3406876835014856 1107 18-19 0.3435419980539933 1107 20-21 0.39742179040438685 1107 22-23 0.4398810164475222 1107 24-25 0.3202308270977525 1107 26-27 0.3530901736503367 1107 28-29 0.30213367843507655 1107 30-31 0.3704466176570591 1107 32-33 0.44621871304526195 1107 34-35 0.40847553017635363 1107 36-37 0.29591716597295914 1107 38-39 0.4851539643071874 1107 40-41 0.563215225981871 1107 42-43 0.5937684067892803 1107 44-45 0.7214227611025379 1107 46-47 0.8854425977312701 1107 48-49 0.7981708011497375 1107 50-51 0.6527116099585868 1107 52-53 0.5597502218314432 1107 54-55 0.8015986053079232 1107 56-57 0.9804851265143313 1107 58-59 0.9452565407292042 1107 60-61 0.876815443656831 1107 62-63 0.9604711520345504 1107 64-65 0.8598753188488928 1107 66-67 0.9781375084915283 1107 68-69 1.170897178674263 1107 70-71 1.2502021680424065 1107 72-73 1.2185866670755487 1107 74-75 1.0748217782202794 1107 76-77 1.3173229144740297 1107 78-79 1.391800402737907 1107 80-81 1.4844773684226098 1107 82-83 1.4746580694575862 1107 84-85 1.2405257025225183 1107 86-87 1.2629895801446374 1107 88-89 1.4707834864046951 1107 90-91 1.4269860335154867 1107 92-93 1.4625561998641459 1107 94-95 1.3792772527389552 1107 96-97 1.3166895875228626 1107 98-99 1.4647597196187512 1107 100-101 1.6677610692056852 1108 1 0.22228639670373695 1108 2 0.2402884055916097 1108 3 0.19657317835896393 1108 4 0.22095635169832661 1108 5 0.237943579855596 1108 6 0.3047407627514218 1108 7 0.3149165706593777 1108 8 0.050107589712474976 1108 9 0.25256239067670805 1108 10-11 0.26361411774153254 1108 12-13 0.32302728881271037 1108 14-15 0.33528398605505316 1108 16-17 0.3684630364942336 1108 18-19 0.31530892025569557 1108 20-21 0.3269314242618506 1108 22-23 0.30728183341355475 1108 24-25 0.3476603684403443 1108 26-27 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0.4882794047803074 2316 66-67 0.3249682993929639 2316 68-69 0.403598521131709 2316 70-71 0.538474941596931 2316 72-73 0.49356565904360394 2316 74-75 0.4896016908975831 2316 76-77 0.5374308032110164 2316 78-79 0.5985627339745854 2316 80-81 0.7853340342918926 2316 82-83 1.100645825806989 2316 84-85 1.1716388413732481 2316 86-87 1.3597697677574558 2316 88-89 1.4462940203677128 2316 90-91 1.5863232513051848 2316 92-93 1.8584727219371011 2316 94-95 2.0927644994473944 2316 96-97 2.080298624891359 2316 98-99 2.0814213169363605 2316 100-101 2.8786759369750534 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10499.0 3 2259.0 4 2021.0 5 2347.0 6 2589.0 7 3165.0 8 3915.0 9 4740.0 10 5977.0 11 7319.0 12 9169.0 13 11440.0 14 12822.0 15 15493.0 16 18736.0 17 22994.0 18 20861.0 19 17232.0 20 18048.0 21 19576.0 22 21619.0 23 24456.0 24 27517.0 25 31098.0 26 35948.0 27 41669.0 28 48777.0 29 57526.0 30 68808.0 31 83585.0 32 103616.0 33 130924.0 34 169764.0 35 227136.0 36 305476.0 37 369693.0 38 237000.0 39 20006.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03391682821202 18.88437276186872 14.81003443383371 23.271675976085554 2 14.822086900095075 19.83613523142301 41.04130965110633 24.300468217375585 3 18.917815775457814 24.09632829705946 30.0388116933745 26.947044234108226 4 12.562202618652755 16.394567211861087 37.79520931800545 33.24802085148072 5 13.84923095023689 36.74012498472761 34.69756139501419 14.713082670021313 6 31.87812827245854 36.007165199926725 17.88753259736236 14.227173930252388 7 28.43587660428364 32.13841758329247 21.089691510709095 18.336014301714794 8 25.979355047672296 35.08886449143292 20.18878926343055 18.74299119746423 9 25.73006842730306 16.90333088276876 20.710105751286548 36.65649493864163 10-11 24.374267333680503 26.61356254431062 28.068556981588955 20.943613140419927 12-13 25.333378875659495 23.83340013879853 28.534567077965146 22.298653907576828 14-15 22.218259088662737 25.271543130365355 26.670293046962378 25.83990473400953 16-17 21.931656341620133 28.71391256576747 27.093832813631913 22.26059827898049 18-19 21.81890301003647 27.17601396480711 29.41169021158655 21.59339281356987 20-21 22.268903667032667 26.375880742565915 29.699809688366113 21.655405902035305 22-23 22.145313773504856 26.274104153644217 29.5172701392991 22.063311933551823 24-25 21.992308951811992 26.460864939874675 29.579303644427878 21.96752246388545 26-27 21.99223958418138 26.70448275051064 29.287292782695694 22.01598488261229 28-29 21.734970628968245 26.690103779681184 29.57138491805266 22.00354067329791 30-31 21.88068785139413 26.474763867216396 29.542058260790487 22.102490020598985 32-33 21.687992960852657 26.603656647040598 29.516036000986208 22.19231439112054 34-35 22.0336981874309 26.550170001863776 29.31783472453184 22.09829708617348 36-37 22.013395497366925 26.41507044339933 29.199854804840292 22.371679254393452 38-39 21.846275609425174 26.63943286147167 29.419881506942247 22.094410022160915 40-41 22.066444294749296 26.468612296574584 29.391964084018323 22.072979324657798 42-43 21.945420556351372 26.51005102518512 29.200576786774796 22.343951631688714 44-45 21.94114487632509 26.753763957597172 29.01897950530035 22.286111660777387 46-47 22.006704228015224 26.877956225973332 28.683112931253053 22.432226614758395 48-49 22.186075316048562 26.916421858898442 28.46237499875598 22.435127826297013 50-51 22.04444382109273 27.023669242743036 28.49817002285011 22.43371691331412 52-53 22.244012362158763 27.082245460345618 28.29337049824206 22.380371679253564 54-55 22.15051628051841 26.88469927346842 28.41276565811149 22.55201878790168 56-57 22.105438587247665 27.29867467408434 28.347895735027983 22.247991003640017 58-59 22.293407242150636 26.93653966641094 28.34503081455937 22.42502227687905 60-61 22.17836436809756 26.82762099668952 28.66171439967386 22.332300235539062 62-63 22.136066829309435 26.958864373884506 28.652910395803517 22.25215840100254 64-65 22.006538493975782 26.923771760027908 28.552561754264506 22.517127991731805 66-67 21.956414677858533 27.11657529296791 28.36991673031848 22.557093298855083 68-69 21.851275661912847 26.994240696814632 28.576635816217856 22.577847825054665 70-71 21.850729947308846 26.94957504724838 28.564770867411937 22.63492413803084 72-73 22.0325396287 26.858286412048027 28.392216719806434 22.716957239445538 74-75 21.886894000296238 27.003404464133276 28.33803129031565 22.77167024525484 76-77 21.985452463134735 26.985246657237287 28.277956469564923 22.751344410063048 78-79 21.958470999289176 27.034378475101846 28.267814176346164 22.739336349262818 80-81 21.873140682295393 27.192041583135172 28.287183244628828 22.647634489940604 82-83 21.95216152326234 27.06126995512288 28.30055776431168 22.686010757303105 84-85 21.99402065571109 26.86361618967495 28.2329461734661 22.90941698114786 86-87 22.056564162916185 26.765858009638766 28.424933271381136 22.75264455606391 88-89 22.141529608443086 26.613532555346588 28.308499550796206 22.936438285414116 90-91 22.06858079399486 26.667565133980926 28.494649672368467 22.76920439965575 92-93 22.064292650142896 26.421988017604093 28.480781085416744 23.03293824683627 94-95 21.863751789199004 26.38524139771209 28.545259668458318 23.205747144630596 96-97 21.919044561831907 26.46943824000517 28.450504329136976 23.16101286902594 98-99 21.7631554249414 26.52259407554991 28.31989814206513 23.394352357443555 100-101 22.197960228872223 25.858694804247772 28.894903511657855 23.04844145522215 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3269.0 1 1826.0 2 374.5 3 346.5 4 285.5 5 269.0 6 388.0 7 822.5 8 1460.5 9 1837.0 10 1826.5 11 1781.5 12 1920.5 13 2081.5 14 2262.0 15 2393.0 16 2491.0 17 2702.0 18 2898.0 19 3152.5 20 3525.0 21 3952.0 22 4668.5 23 5650.0 24 6875.0 25 8643.0 26 11295.5 27 14638.0 28 17625.0 29 21269.0 30 26318.0 31 30708.5 32 34551.0 33 38742.0 34 43963.0 35 48996.0 36 54173.0 37 59266.5 38 62490.5 39 64572.5 40 67088.0 41 72543.0 42 78048.0 43 82492.0 44 86761.0 45 88629.5 46 88078.5 47 88529.5 48 91711.0 49 91597.5 50 87397.0 51 82306.5 52 76871.5 53 72488.0 54 68290.5 55 62866.5 56 56589.0 57 50684.5 58 45219.0 59 39836.5 60 34154.5 61 27300.0 62 20581.5 63 15717.5 64 12088.0 65 9218.5 66 6803.5 67 5068.0 68 3657.5 69 2444.5 70 1742.0 71 1237.5 72 896.5 73 661.5 74 462.5 75 343.0 76 233.5 77 141.5 78 94.0 79 59.5 80 40.0 81 32.5 82 29.0 83 20.5 84 14.5 85 14.5 86 11.0 87 10.5 88 9.0 89 7.0 90 6.0 91 4.0 92 4.5 93 5.0 94 3.0 95 1.5 96 0.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31356309306433455 2 0.26856850414879896 3 0.2672146047832362 4 0.26658278507930694 5 0.26861363412765105 6 0.23183270136319611 7 0.2336830304961319 8 0.23508205984054675 9 0.24582299480734462 10-11 0.2515093721427082 12-13 0.24478500529374653 14-15 0.23329942567588913 16-17 0.2300500671985385 18-19 0.21822601273929046 20-21 0.2244990797997312 22-23 0.220956376459842 24-25 0.22294209552933403 26-27 0.2192414372634625 28-29 0.23582670449160628 30-31 0.24907235328469526 32-33 0.24040739734509362 34-35 0.2372934288042993 36-37 0.22585297916529398 38-39 0.21310376013957802 40-41 0.21037339641902644 42-43 0.22352878525441125 44-45 0.22043738170304294 46-47 0.23140396656410128 48-49 0.23666160910036996 50-51 0.250403687660832 52-53 0.26410063624244184 54-55 0.23695495396290855 56-57 0.21235911548851846 58-59 0.20076071092353084 60-61 0.20360389959121267 62-63 0.20924514694772414 64-65 0.2011217507543476 66-67 0.20637939329061628 68-69 0.20836511236010835 70-71 0.20917745197944598 72-73 0.21084726119697342 74-75 0.214570484452271 76-77 0.22569502423931165 78-79 0.22589810914414604 80-81 0.21795523286617788 82-83 0.21089239117582548 84-85 0.19746622246732815 86-87 0.20035454111386203 88-89 0.21154677586918083 90-91 0.20980927168337526 92-93 0.21414174965317612 94-95 0.20926771193715016 96-97 0.2173234131622486 98-99 0.22111433138582431 100-101 0.2050931888933317 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2215822.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.748174056303846 #Duplication Level Percentage of deduplicated Percentage of total 1 77.90734210265899 43.43192067803043 2 12.497684000350711 13.934461259044706 3 4.055241995290664 6.7821700978169055 4 1.7857813211703437 3.982161916764023 5 0.9514779111791068 2.6521578101570635 6 0.5653708355433085 1.8911035047735785 7 0.39679819313373976 1.548454231523262 8 0.270214547775374 1.2051174113541587 9 0.19771737922711885 0.992014458399869 >10 1.210907566906428 12.54131836715219 >50 0.10352618809976644 3.9947554503023572 >100 0.05526625286221891 5.231551374583825 >500 0.00210498032899772 0.7566290180454736 >1k 4.048039094226276E-4 0.4842895110729988 >5k 1.6192156376905104E-4 0.5718949109791209 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7229 0.3262446171217724 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5442 0.24559734491308416 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3697 0.16684553181618378 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3417 0.15420913773759806 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.025995770418382E-5 2 0.0 0.0 0.0 0.0 9.025995770418382E-5 3 0.0 0.0 0.0 0.0 9.025995770418382E-5 4 0.0 0.0 0.0 0.0 9.025995770418382E-5 5 0.0 4.512997885209191E-5 0.0 0.0 9.025995770418382E-5 6 0.0 4.512997885209191E-5 0.0 0.0 9.025995770418382E-5 7 0.0 4.512997885209191E-5 0.0 0.0 9.025995770418382E-5 8 0.0 4.512997885209191E-5 0.0 0.0 9.025995770418382E-5 9 0.0 4.512997885209191E-5 0.0 0.0 9.025995770418382E-5 10-11 0.0 4.512997885209191E-5 0.0 0.0 9.025995770418382E-5 12-13 0.0 4.512997885209191E-5 0.0 0.0 9.025995770418382E-5 14-15 0.0 4.512997885209191E-5 0.0 0.0 9.025995770418382E-5 16-17 0.0 4.512997885209191E-5 0.0 0.0 9.025995770418382E-5 18-19 0.0 4.512997885209191E-5 0.0 0.0 9.025995770418382E-5 20-21 0.0 4.512997885209191E-5 0.0 2.2564989426045956E-5 1.1282494713022977E-4 22-23 0.0 4.512997885209191E-5 0.0 1.579549259823217E-4 1.579549259823217E-4 24-25 0.0 4.512997885209191E-5 0.0 2.7077987311255146E-4 1.8051991540836765E-4 26-27 0.0 4.512997885209191E-5 0.0 4.512997885209191E-4 1.8051991540836765E-4 28-29 6.769496827813786E-5 4.512997885209191E-5 0.0 0.001015424524172068 1.8051991540836765E-4 30-31 9.025995770418382E-5 4.512997885209191E-5 0.0 0.002527278815717147 1.8051991540836765E-4 32-33 9.025995770418382E-5 4.512997885209191E-5 0.0 0.004625822832339421 1.8051991540836765E-4 34-35 1.1282494713022977E-4 4.512997885209191E-5 0.0 0.0064535869758491436 1.8051991540836765E-4 36-37 1.3538993655627573E-4 4.512997885209191E-5 0.0 0.009522425537791392 1.8051991540836765E-4 38-39 1.3538993655627573E-4 4.512997885209191E-5 0.0 0.012185094290064816 1.8051991540836765E-4 40-41 1.3538993655627573E-4 4.512997885209191E-5 0.0 0.01617909741847495 1.8051991540836765E-4 42-43 1.579549259823217E-4 4.512997885209191E-5 0.0 0.021955734711542715 1.8051991540836765E-4 44-45 1.8051991540836765E-4 4.512997885209191E-5 0.0 0.031861765069576886 2.0308490483441358E-4 46-47 3.610398308167353E-4 4.512997885209191E-5 0.0 0.05666068844880139 2.2564989426045954E-4 48-49 3.610398308167353E-4 4.512997885209191E-5 0.0 0.1035507364761249 2.2564989426045954E-4 50-51 3.610398308167353E-4 4.512997885209191E-5 0.0 0.13728539566806358 2.2564989426045954E-4 52-53 3.610398308167353E-4 4.512997885209191E-5 0.0 0.16907946576936234 2.2564989426045954E-4 54-55 3.610398308167353E-4 4.512997885209191E-5 0.0 0.21190781569999756 2.2564989426045954E-4 56-57 3.610398308167353E-4 4.512997885209191E-5 0.0 0.2468384193315167 2.2564989426045954E-4 58-59 3.610398308167353E-4 4.512997885209191E-5 0.0 0.2940218122213788 2.2564989426045954E-4 60-61 3.610398308167353E-4 4.512997885209191E-5 0.0 0.36909553204183365 2.2564989426045954E-4 62-63 3.610398308167353E-4 4.512997885209191E-5 0.0 0.4283286292852043 2.2564989426045954E-4 64-65 3.610398308167353E-4 4.512997885209191E-5 0.0 0.4980093166328342 2.7077987311255146E-4 66-67 3.610398308167353E-4 4.512997885209191E-5 0.0 0.6158211264262201 2.7077987311255146E-4 68-69 3.610398308167353E-4 4.512997885209191E-5 0.0 0.7474201447589202 2.7077987311255146E-4 70-71 3.610398308167353E-4 4.512997885209191E-5 0.0 0.8650739996263238 2.7077987311255146E-4 72-73 3.610398308167353E-4 4.512997885209191E-5 0.0 1.0136193250179844 2.933448625385974E-4 74-75 3.836048202427812E-4 4.512997885209191E-5 0.0 1.1476553622086971 3.159098519646434E-4 76-77 4.0616980966882716E-4 4.512997885209191E-5 0.0 1.2658959068011781 3.159098519646434E-4 78-79 4.0616980966882716E-4 4.512997885209191E-5 0.0 1.4163818212834784 3.159098519646434E-4 80-81 4.0616980966882716E-4 4.512997885209191E-5 0.0 1.605115392842927 3.159098519646434E-4 82-83 4.0616980966882716E-4 4.512997885209191E-5 0.0 1.7998963815685556 3.159098519646434E-4 84-85 4.512997885209191E-4 4.512997885209191E-5 0.0 2.030375183566189 3.159098519646434E-4 86-87 5.18994756799057E-4 4.512997885209191E-5 0.0 2.390692934721291 3.159098519646434E-4 88-89 5.415597462251029E-4 4.512997885209191E-5 0.0 2.893756808985559 3.159098519646434E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3870 0.0 50.364594 1 GTATCAA 6910 0.0 39.83391 1 ATCAACG 8280 0.0 32.648922 3 TATCAAC 8420 0.0 32.163948 2 TCAACGC 8420 0.0 32.106792 4 CAACGCA 8710 0.0 31.039202 5 AACGCAG 8865 0.0 30.490973 6 ACGCAGA 10210 0.0 26.28937 7 GTACATG 7580 0.0 26.090149 1 CGCAGAG 10220 0.0 25.98474 8 ACATGGG 7570 0.0 24.727901 3 TACATGG 7830 0.0 24.636038 2 GCAGAGT 11110 0.0 23.475548 9 TGGTATC 2080 0.0 22.842482 2 GAGTACT 6100 0.0 22.270994 12-13 TAAACCG 135 3.7902282E-4 21.117083 5 GTGGTAT 2440 0.0 20.847136 1 CAGAGTA 10680 0.0 20.393702 10-11 AGAGTAC 10015 0.0 19.992846 10-11 GTACTTT 6890 0.0 19.85441 14-15 >>END_MODULE