FastQCFastQC Report
Thu 26 May 2016
SRR937447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937447_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215822
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74320.33540600282874705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60740.2741194915476063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38980.17591665756545427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40750.045.4711951
GTATCAA69400.038.6117061
ATCAACG88950.030.0104223
TCAACGC90300.029.5617624
TATCAAC90900.029.5233972
CAACGCA93500.028.5500225
AACGCAG94850.028.2431876
ACGCAGA109800.024.1852197
GTACATG71700.024.0539741
TACCGTA1000.001653088323.7525677
CGCAGAG111900.023.6888878
TACATGG74350.022.8070432
ACATGGG75100.021.6305683
GCAGAGT120750.021.5199249
TGGTATC23150.020.9281562
GAGTACT69650.020.59806312-13
AGAGTAC108550.020.1530310-11
TACACCG2401.4150646E-719.7911285
AGTACTT74600.019.6452212-13
CATGGGG41000.019.2311824
Produced by FastQC (version 0.11.5)