##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937447_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2215822 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.282467635035665 33.0 31.0 34.0 31.0 34.0 2 32.49476131205485 34.0 31.0 34.0 31.0 34.0 3 32.56580492476381 34.0 31.0 34.0 31.0 34.0 4 36.16959259362891 37.0 35.0 37.0 35.0 37.0 5 36.09627939428348 37.0 35.0 37.0 35.0 37.0 6 36.12922202234656 37.0 35.0 37.0 35.0 37.0 7 36.07181578664712 37.0 35.0 37.0 35.0 37.0 8 36.04759723479594 37.0 35.0 37.0 35.0 37.0 9 37.75519604011514 39.0 37.0 39.0 35.0 39.0 10-11 37.67592726311048 39.0 37.0 39.0 35.0 39.0 12-13 37.5672089184059 39.0 37.0 39.0 35.0 39.0 14-15 38.83441382024368 40.0 38.0 41.0 35.0 41.0 16-17 38.789550785216505 40.0 38.0 41.0 35.0 41.0 18-19 38.74495063231613 40.0 38.0 41.0 35.0 41.0 20-21 38.63047099451129 40.0 38.0 41.0 34.0 41.0 22-23 38.57273305346729 40.0 38.0 41.0 34.0 41.0 24-25 38.61436771545729 40.0 38.0 41.0 34.0 41.0 26-27 38.471207299142264 40.0 38.0 41.0 34.0 41.0 28-29 38.326538187634206 40.0 38.0 41.0 34.0 41.0 30-31 38.40690926437232 40.0 38.0 41.0 34.0 41.0 32-33 38.34069275420138 40.0 38.0 41.0 34.0 41.0 34-35 38.2357522400265 40.0 38.0 41.0 34.0 41.0 36-37 38.08384405426068 40.0 38.0 41.0 33.5 41.0 38-39 37.98766462288036 40.0 38.0 41.0 33.0 41.0 40-41 37.81354820017131 40.0 37.0 41.0 33.0 41.0 42-43 37.635758422833604 40.0 37.0 41.0 33.0 41.0 44-45 37.37872672082866 40.0 37.0 41.0 32.5 41.0 46-47 37.07919724598817 40.0 37.0 41.0 31.5 41.0 48-49 36.792171257438554 39.5 36.0 41.0 31.0 41.0 50-51 36.56147944194073 39.0 36.0 41.0 30.5 41.0 52-53 36.32305347631714 39.0 36.0 40.5 30.0 41.0 54-55 36.10751382556902 39.0 35.0 40.0 30.0 41.0 56-57 35.814029285745875 38.5 35.0 40.0 29.0 41.0 58-59 35.54106309080784 38.0 35.0 40.0 28.0 41.0 60-61 35.199543781043786 38.0 34.0 40.0 28.0 41.0 62-63 34.81672625328208 37.5 34.0 40.0 27.5 41.0 64-65 34.39394048799949 37.0 34.0 39.5 26.0 41.0 66-67 33.95208617840242 36.5 33.0 39.0 26.0 40.5 68-69 33.495645182690666 36.0 33.0 39.0 26.0 40.0 70-71 33.04650982795549 35.5 32.0 38.5 25.0 40.0 72-73 32.558806844593114 35.0 32.0 37.5 23.5 39.5 74-75 32.06179377224344 35.0 31.0 37.0 22.5 39.0 76-77 30.75733023681505 33.5 29.5 35.5 21.5 38.0 78-79 31.403064867123803 34.0 31.0 36.0 22.0 38.5 80-81 31.203106115924474 34.0 31.0 36.0 21.0 37.5 82-83 30.789490987994522 34.0 31.0 35.0 20.0 37.0 84-85 30.341759401251544 34.0 30.0 35.0 18.0 36.5 86-87 29.943827617922377 34.0 30.0 35.0 15.5 36.0 88-89 29.494238481249845 34.0 29.5 35.0 8.5 36.0 90-91 28.927009931303147 34.0 29.0 35.0 4.5 35.0 92-93 28.40379552148142 33.0 29.0 35.0 2.0 35.0 94-95 27.90074383231144 33.0 27.5 35.0 2.0 35.0 96-97 27.211281637243424 33.0 26.5 35.0 2.0 35.0 98-99 26.20489822738469 32.5 25.0 34.0 2.0 35.0 100-101 23.20947846893839 29.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24345381373095165 1101 2 0.2457578517199508 1101 3 0.20473267895368963 1101 4 0.16554708683194974 1101 5 0.14580274733391008 1101 6 0.1820786596188455 1101 7 0.1600965282257789 1101 8 0.1000614276668017 1101 9 0.1975052118985161 1101 10-11 0.15421788205897968 1101 12-13 0.2583335520447818 1101 14-15 0.3722993105995158 1101 16-17 0.2546514317946986 1101 18-19 0.3362242710898613 1101 20-21 0.3677330124109872 1101 22-23 0.36849705958149315 1101 24-25 0.2777657839245151 1101 26-27 0.22640447739382807 1101 28-29 0.07118731855732818 1101 30-31 0.2703262940899691 1101 32-33 0.2841303710394456 1101 34-35 0.3779104586496089 1101 36-37 0.34260692026085593 1101 38-39 0.13240472938888104 1101 40-41 0.215336356454074 1101 42-43 0.26102623415804516 1101 44-45 0.25185492453595515 1101 46-47 0.3811942064687628 1101 48-49 0.2596409119224248 1101 50-51 0.1980163983878782 1101 52-53 0.10456307511336149 1101 54-55 0.09367757012850575 1101 56-57 0.213322015910407 1101 58-59 0.2102744649265702 1101 60-61 0.23364696052750844 1101 62-63 0.3348865159964234 1101 64-65 0.26392867744626614 1101 66-67 0.2526922641253364 1101 68-69 0.34188702440589225 1101 70-71 0.37213441483996945 1101 72-73 0.3659309027539521 1101 74-75 0.35873903296834087 1101 76-77 0.31092077526187367 1101 78-79 0.2350115490380027 1101 80-81 0.2640347257986555 1101 82-83 0.3474037862823671 1101 84-85 0.5197328220812238 1101 86-87 0.709314565266272 1101 88-89 0.8922448088700783 1101 90-91 0.9695266423591384 1101 92-93 1.0742408857342092 1101 94-95 1.169145559686477 1101 96-97 1.351892680809609 1101 98-99 1.9473808688678034 1101 100-101 2.8276959057644184 1102 1 0.2765736050807348 1102 2 0.30114363238781294 1102 3 0.2608811360391172 1102 4 0.15123542652462163 1102 5 0.15030255408336757 1102 6 0.17440741525214776 1102 7 0.13925549359747436 1102 8 0.22257369427089913 1102 9 0.31686780440738005 1102 10-11 0.2047440430038847 1102 12-13 0.17821535546870848 1102 14-15 0.37256952775975094 1102 16-17 0.38439951346732215 1102 18-19 0.38979319481380514 1102 20-21 0.35261602236008827 1102 22-23 0.3985239685084636 1102 24-25 0.29251549468413174 1102 26-27 0.27860555581605695 1102 28-29 0.24898600025978368 1102 30-31 0.36338989424990586 1102 32-33 0.41480499097607293 1102 34-35 0.3968129512400864 1102 36-37 0.41384718843193014 1102 38-39 0.43330083049362145 1102 40-41 0.2712188061116336 1102 42-43 0.40620298493410445 1102 44-45 0.38461971630432856 1102 46-47 0.3449930498884797 1102 48-49 0.5360126214407259 1102 50-51 0.41778611173052127 1102 52-53 0.4021664506515137 1102 54-55 0.4778733928984522 1102 56-57 0.48825209066730935 1102 58-59 0.6100792604312772 1102 60-61 0.6647591384571641 1102 62-63 0.6386031377831074 1102 64-65 0.7571926290950302 1102 66-67 0.6332780428233562 1102 68-69 0.6025322063989833 1102 70-71 0.6981452232086625 1102 72-73 0.7409623764605158 1102 74-75 0.6736050911989402 1102 76-77 0.5857820645805738 1102 78-79 0.7065103900907381 1102 80-81 0.5599749393385203 1102 82-83 0.724101201999158 1102 84-85 0.7941333442786664 1102 86-87 0.7852625056507456 1102 88-89 0.8560725644139389 1102 90-91 0.9940671580148148 1102 92-93 1.0991204834172343 1102 94-95 1.0491766862886962 1102 96-97 1.2163566142850364 1102 98-99 1.5111845256796173 1102 100-101 2.334763331538497 1103 1 0.16959491081819067 1103 2 0.1815876278689572 1103 3 0.1523459471173183 1103 4 0.10851840917416666 1103 5 0.092667336385027 1103 6 0.019688874538466905 1103 7 0.09970116272878471 1103 8 0.09808400814008422 1103 9 0.1832997615619405 1103 10-11 0.22843310954257845 1103 12-13 0.2090495419936076 1103 14-15 0.22003834535152578 1103 16-17 0.20150020487270126 1103 18-19 0.26312622643469297 1103 20-21 0.3282253036041922 1103 22-23 0.250586358088114 1103 24-25 0.21362372434651178 1103 26-27 0.2919501834416991 1103 28-29 0.21449830372329615 1103 30-31 0.18764766028556323 1103 32-33 0.17865483039958718 1103 34-35 0.2187618485522549 1103 36-37 0.17935359996371858 1103 38-39 0.23445539294084483 1103 40-41 0.2762953457203565 1103 42-43 0.36069071562670985 1103 44-45 0.3224734136622658 1103 46-47 0.30718221846860416 1103 48-49 0.3003947840870751 1103 50-51 0.18683365961953768 1103 52-53 0.10400072000501837 1103 54-55 0.17461763097347927 1103 56-57 0.06371415900859034 1103 58-59 0.055530561053856786 1103 60-61 0.23097693670205643 1103 62-63 0.26095174715913316 1103 64-65 0.3993177207828822 1103 66-67 0.3885983290858448 1103 68-69 0.41918374474819586 1103 70-71 0.3952442580419344 1103 72-73 0.3350821432083322 1103 74-75 0.4281565230664377 1103 76-77 0.23767700375278267 1103 78-79 0.30108366480077464 1103 80-81 0.5438044046329082 1103 82-83 0.5407933861382439 1103 84-85 0.6711782074961867 1103 86-87 0.7115354831170961 1103 88-89 0.791735060902262 1103 90-91 1.0824063420078893 1103 92-93 1.0021351030930425 1103 94-95 1.0456387947634305 1103 96-97 1.0649647094576657 1103 98-99 1.4603147826276128 1103 100-101 2.0992299452062433 1104 1 0.18338739622169697 1104 2 0.23835139034129327 1104 3 0.17748574039067222 1104 4 0.1598759299423662 1104 5 0.07949895375980276 1104 6 0.1343842883722104 1104 7 0.17072037278477126 1104 8 0.09723772409638087 1104 9 0.13171667777390184 1104 10-11 0.10691475253414495 1104 12-13 0.15651381798534203 1104 14-15 0.22889997917854288 1104 16-17 0.2007262903735949 1104 18-19 0.1938643450632469 1104 20-21 0.2807195165769443 1104 22-23 0.15700027806565942 1104 24-25 0.18206908111314846 1104 26-27 0.19025846079291142 1104 28-29 0.09027826310842357 1104 30-31 0.2238869633736158 1104 32-33 0.16831959708390087 1104 34-35 0.1336400462124132 1104 36-37 0.12973210282721936 1104 38-39 0.07627636624358303 1104 40-41 0.11800886377638875 1104 42-43 -0.018051322455228558 1104 44-45 0.08113205768020038 1104 46-47 0.020607276254331452 1104 48-49 0.02491317998568121 1104 50-51 -0.010420440334982572 1104 52-53 -0.038124864827139504 1104 54-55 -0.033847492147735636 1104 56-57 -0.022188828602345723 1104 58-59 -0.04296886996596072 1104 60-61 0.0903663778502306 1104 62-63 0.1899390469804132 1104 64-65 -0.047963010487691804 1104 66-67 0.14638076053345372 1104 68-69 0.2666519097575275 1104 70-71 0.08591070038555415 1104 72-73 0.1362032793425456 1104 74-75 0.19305999934089257 1104 76-77 0.20406397114759756 1104 78-79 0.11880363524424098 1104 80-81 0.10566235742023622 1104 82-83 0.0898351660232315 1104 84-85 0.1938806858149178 1104 86-87 0.28223714643300823 1104 88-89 0.48291891167220413 1104 90-91 0.6083668870790646 1104 92-93 0.6268012816598194 1104 94-95 0.7974012806234683 1104 96-97 0.9821370893166126 1104 98-99 1.2992293070937606 1104 100-101 2.0255440527020703 1105 1 0.1368089871129783 1105 2 0.09358980219685265 1105 3 0.11561731926526875 1105 4 0.09380766004716179 1105 5 0.10353616708071911 1105 6 0.07936178358597346 1105 7 0.06501716312201467 1105 8 0.14450822182562462 1105 9 0.23077875558831806 1105 10-11 0.16219558074223528 1105 12-13 0.15803825647180503 1105 14-15 0.22751557149732093 1105 16-17 0.19810265231336643 1105 18-19 0.2951921105525912 1105 20-21 0.3048185646747257 1105 22-23 0.3161169824886656 1105 24-25 0.20886804296485906 1105 26-27 0.18374069124475056 1105 28-29 0.15810778716346618 1105 30-31 0.1689316703013546 1105 32-33 0.12566996230082594 1105 34-35 0.19406744819008992 1105 36-37 0.2675517578982394 1105 38-39 0.19929976575215846 1105 40-41 0.1965079209688696 1105 42-43 0.08899374027212303 1105 44-45 0.24535193630890006 1105 46-47 0.1857218004061565 1105 48-49 0.1947434316171197 1105 50-51 0.2591700459890163 1105 52-53 0.1326378245140205 1105 54-55 0.05295985465333786 1105 56-57 0.2631085359917833 1105 58-59 0.1859500031140442 1105 60-61 0.12623984284990541 1105 62-63 0.2988729509040695 1105 64-65 0.2786709408451884 1105 66-67 0.3317094189016174 1105 68-69 0.453054188841449 1105 70-71 0.46284635934149065 1105 72-73 0.310570974581438 1105 74-75 0.4238101803900527 1105 76-77 0.4687775755051895 1105 78-79 0.41049333312490077 1105 80-81 0.4922769640696316 1105 82-83 0.4894868480367016 1105 84-85 0.6177780526827199 1105 86-87 0.778142532003951 1105 88-89 0.9622972056222885 1105 90-91 1.1381844614290486 1105 92-93 1.2195098169483494 1105 94-95 1.3013252157057096 1105 96-97 1.3011696646451973 1105 98-99 1.6344036012785885 1105 100-101 2.0729038075874797 1106 1 0.017049241481480237 1106 2 0.1453302084025836 1106 3 0.11536675043662825 1106 4 0.09744539586234424 1106 5 0.04684384765108973 1106 6 0.08268518641538236 1106 7 0.0711931237729857 1106 8 0.010534304918735415 1106 9 0.06115805695754517 1106 10-11 -0.14427477958322044 1106 12-13 -0.22625775357488465 1106 14-15 -0.038053915352669776 1106 16-17 0.015579902907617793 1106 18-19 0.14691361190117647 1106 20-21 0.2285768278363136 1106 22-23 0.2494345399586777 1106 24-25 0.06702299248458132 1106 26-27 0.23096721219754102 1106 28-29 0.1724587428404547 1106 30-31 0.2560292081447244 1106 32-33 0.3087793045539158 1106 34-35 -0.08012955351079398 1106 36-37 0.38954708235868907 1106 38-39 0.42102615880265404 1106 40-41 0.39656777700025003 1106 42-43 0.31401146708179084 1106 44-45 0.30051251352971775 1106 46-47 0.5642091014283181 1106 48-49 0.6204129365679165 1106 50-51 0.5914963980509356 1106 52-53 0.47723877682516047 1106 54-55 0.5420728260406165 1106 56-57 0.43861115088319735 1106 58-59 0.571607031981074 1106 60-61 0.5766873325990218 1106 62-63 0.7180067232439598 1106 64-65 0.6750222099202077 1106 66-67 0.6157751861134457 1106 68-69 0.7709838657647339 1106 70-71 0.7992277191149455 1106 72-73 0.8512140800492247 1106 74-75 0.8994208577636051 1106 76-77 0.753871609872764 1106 78-79 0.9631896407127236 1106 80-81 0.8537479302480762 1106 82-83 0.8790346124391739 1106 84-85 0.9373440001545212 1106 86-87 1.1014831866842023 1106 88-89 1.232805048800607 1106 90-91 1.2643439032364938 1106 92-93 1.4754300664999427 1106 94-95 1.1983652685719939 1106 96-97 1.4363990435785645 1106 98-99 1.6764200670164797 1106 100-101 2.1776529844501766 1107 1 0.032654132086371135 1107 2 0.07502776310013815 1107 3 0.0512311863010666 1107 4 0.09497864339559214 1107 5 0.03102445683170174 1107 6 0.05989453862473226 1107 7 0.028507581087446 1107 8 0.012572056956486222 1107 9 0.13038800118749094 1107 10-11 -0.009997645306086156 1107 12-13 0.04296682814970154 1107 14-15 0.04458229228352906 1107 16-17 0.04888106120878177 1107 18-19 0.09899962648719196 1107 20-21 0.23029282955231878 1107 22-23 0.22908383210798178 1107 24-25 0.03964740260899191 1107 26-27 0.07194211567244224 1107 28-29 0.26767002555173036 1107 30-31 0.15247922959474636 1107 32-33 0.15508990086450325 1107 34-35 0.22046568458444682 1107 36-37 0.15979054010215066 1107 38-39 0.13029793057442873 1107 40-41 0.1054641733966406 1107 42-43 0.16616719423751647 1107 44-45 0.17312619864340917 1107 46-47 0.024499749218975353 1107 48-49 -0.05574536459038626 1107 50-51 -0.1180434989889676 1107 52-53 -0.025978726392338558 1107 54-55 -0.03525450128670826 1107 56-57 0.051625951398001746 1107 58-59 0.1148824502564878 1107 60-61 0.3272503956620838 1107 62-63 0.2842336644709036 1107 64-65 0.37193340683140974 1107 66-67 0.2927378630761268 1107 68-69 0.21175499403586429 1107 70-71 0.3361753810626098 1107 72-73 0.33070249703764176 1107 74-75 0.3173745257172733 1107 76-77 0.34637482737597836 1107 78-79 0.2369455394686213 1107 80-81 0.48019580669595285 1107 82-83 0.5937493271538905 1107 84-85 0.6949051327156504 1107 86-87 0.7080093557103702 1107 88-89 0.9603629638585254 1107 90-91 0.8951085965011885 1107 92-93 0.9477327263026041 1107 94-95 0.8722445049512295 1107 96-97 1.1152922224717479 1107 98-99 1.3473231129195256 1107 100-101 2.1374878192850133 1108 1 0.11363001480138024 1108 2 0.1405752982128945 1108 3 0.1447652172264 1108 4 0.09651259971215609 1108 5 0.08357738242810342 1108 6 0.1293381437205099 1108 7 0.12328757847613758 1108 8 0.18389841436979992 1108 9 0.18374968585352036 1108 10-11 0.14036290157620357 1108 12-13 0.15186280487176163 1108 14-15 0.24788111906061516 1108 16-17 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0.7871523453689839 2316 94-95 1.0119072362876373 2316 96-97 1.3388039716353042 2316 98-99 1.707796406109388 2316 100-101 2.047148050223626 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 4.0 4 9.0 5 43.0 6 76.0 7 165.0 8 413.0 9 843.0 10 1390.0 11 2303.0 12 3552.0 13 5034.0 14 6930.0 15 9309.0 16 12863.0 17 18113.0 18 16725.0 19 10808.0 20 10175.0 21 10332.0 22 10901.0 23 12378.0 24 14313.0 25 17254.0 26 20935.0 27 26106.0 28 32628.0 29 40653.0 30 51552.0 31 65497.0 32 84511.0 33 111737.0 34 153698.0 35 223296.0 36 343701.0 37 505362.0 38 365653.0 39 26554.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.329037775086846 19.885490909977488 15.01391067369921 22.77156064123646 2 15.489969632023525 20.795993900229305 39.61628669463824 24.09774977310893 3 19.336480998925005 24.566865027967047 29.977678712459756 26.11897526064819 4 13.324761646016691 17.282254621535483 36.809409781110574 32.58357395133725 5 13.998316631276811 37.4040017239862 34.17604065358032 14.421640991156673 6 32.34591948270213 36.64694185724305 17.082509335136127 13.924629324918698 7 28.744953340114865 32.50965104597752 20.57281676957806 18.172578844329554 8 26.459435821108375 35.68797493661495 19.554639316696015 18.297949925580664 9 26.05732770953624 17.064186563722174 20.346309405719413 36.53217632102218 10-11 24.689799090360147 27.181741132636105 27.394732067828553 20.733727709175195 12-13 25.64643514865103 24.203511335759494 28.167456067683965 21.982597447905512 14-15 22.4231233375244 25.839801211469155 26.165910438654365 25.571165012352076 16-17 22.11242599811718 29.413824756681716 26.55357695699384 21.920172288207265 18-19 22.091621077866364 27.678757589734193 28.93066320309122 21.29895812930822 20-21 22.53464968515056 26.901992534595532 29.301689983080763 21.261667797173146 22-23 22.46984679865304 26.835384540051844 29.04967687881111 21.64509178248401 24-25 22.35637371266619 27.04071179066892 29.08839616936394 21.514518327300955 26-27 22.31712705237462 27.32988324238747 28.904808991116926 21.448180714120987 28-29 22.034711053754048 27.267048915051088 29.10651677908536 21.591723252109503 30-31 22.179930350793622 27.012839724914294 29.1663554573626 21.64087446692949 32-33 22.010447145876665 27.206671298509967 29.085614109987702 21.697267445625673 34-35 22.42131813837032 27.05406842246354 28.860305566060813 21.664307873105333 36-37 22.387696304125512 26.993887595664273 28.698604851833764 21.91981124837645 38-39 22.15695172196858 27.22013032186934 28.932813405772983 21.6901045503891 40-41 22.42560548636127 27.110322941102673 28.825916522175515 21.638155050360545 42-43 22.290240918123764 27.227643006631464 28.63212990866333 21.849986166581438 44-45 22.33947492172205 27.38218593370767 28.452826987005274 21.825512157565 46-47 22.389569198247873 27.59156647059195 28.063287574543445 21.955576756616733 48-49 22.596377326337585 27.695636201824875 27.83768281026184 21.870303661575704 50-51 22.489960836204354 27.746497687991184 27.81448600113186 21.949055474672605 52-53 22.67560812096101 27.874876202798827 27.5533475959142 21.896168080325964 54-55 22.473804303775303 27.700803584403438 27.76624205373897 22.059150058082285 56-57 22.375578904803724 28.153118797448535 27.703624208081695 21.76767808966605 58-59 22.674339364804574 27.705970966982008 27.609573332153936 22.010116336059486 60-61 22.53364665573318 27.697847570788632 27.984242416584003 21.78426335689419 62-63 22.487207907494376 27.799141808322148 27.964317530920805 21.74933275326267 64-65 22.43894139511206 27.690626774172294 27.868303500912976 22.002128329802666 66-67 22.29215613889563 28.01077884414903 27.67828372495625 22.018781291999087 68-69 22.302851943883578 27.7393220213537 27.836735080705942 22.12109095405678 70-71 22.24165569256014 27.823444301934003 27.786889019063803 22.148010986442053 72-73 22.367205211805764 27.80962584655627 27.636393914565538 22.186775027072425 74-75 22.295653712256673 27.997081895567423 27.51859129478812 22.188673097387788 76-77 22.43187855342171 27.89113927021214 27.553115728609967 22.123866447756182 78-79 22.356308403833882 27.90503930369858 27.493747241430043 22.24490505103749 80-81 22.267131565622147 28.196917441924484 27.471768941729074 22.06418205072429 82-83 22.3533603557518 28.104473546860532 27.45006407350435 22.092102023883317 84-85 22.301909246751148 27.946068760502595 27.363418037057585 22.38860395568867 86-87 22.28983194498475 27.829243504216493 27.582472779853255 22.298451770945498 88-89 22.32162601508605 27.727001537127077 27.477184539191324 22.474187908595546 90-91 22.283478622331277 27.84672069014775 27.528280184316824 22.34152050320415 92-93 22.170505201851704 27.641876119094817 27.582145931656658 22.605472747396817 94-95 22.171117535614325 27.520825228741298 27.617786988305014 22.690270247339363 96-97 22.13769878627435 27.722691624146705 27.552619298842597 22.58699029073635 98-99 21.95853939939467 27.80571207562727 27.33838956284472 22.89735896213334 100-101 21.97781217552298 27.926107083706352 27.52659625484832 22.569484485922338 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 216.0 1 165.5 2 137.5 3 180.5 4 185.5 5 198.0 6 338.5 7 724.5 8 1287.5 9 1720.0 10 1813.5 11 1825.5 12 1974.0 13 2215.5 14 2448.0 15 2663.0 16 2820.5 17 2945.0 18 3108.5 19 3309.5 20 3655.0 21 4149.0 22 4864.5 23 5804.5 24 6938.0 25 8641.0 26 11128.0 27 14302.0 28 17462.5 29 21377.5 30 26377.5 31 30540.0 32 34572.5 33 39125.0 34 44764.5 35 49706.0 36 54490.5 37 59922.5 38 63060.5 39 65199.5 40 67671.5 41 72864.0 42 78674.0 43 83124.5 44 87938.5 45 89669.5 46 88503.0 47 89354.0 48 92646.0 49 91924.5 50 88134.0 51 83277.0 52 77220.5 53 72136.0 54 67869.5 55 62657.5 56 55691.0 57 49316.5 58 44001.0 59 39126.5 60 33563.5 61 26553.0 62 20072.0 63 15373.5 64 11408.5 65 8714.5 66 6657.5 67 5051.5 68 3448.0 69 2012.5 70 1421.5 71 1026.5 72 773.0 73 574.0 74 410.0 75 282.0 76 177.5 77 107.5 78 61.5 79 32.0 80 20.0 81 11.0 82 8.0 83 4.5 84 2.0 85 1.5 86 1.5 87 1.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044046859359641706 2 4.512997885209191E-5 3 0.0 4 0.0 5 0.0012185094290064816 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00507712262086034 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.512997885209191E-5 22-23 5.641247356511489E-4 24-25 9.025995770418382E-5 26-27 0.0015795492598232167 28-29 0.0032719234667766633 30-31 1.579549259823217E-4 32-33 6.769496827813786E-5 34-35 0.0 36-37 0.0 38-39 2.2564989426045956E-5 40-41 0.0 42-43 0.007807486341411901 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.2564989426045956E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.933448625385974E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.3847484139068934E-4 84-85 2.2564989426045956E-5 86-87 0.0 88-89 0.0 90-91 0.00814596118280259 92-93 0.0011056844818762518 94-95 0.0 96-97 0.0 98-99 3.3847484139068934E-4 100-101 0.010718369977371828 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2215822.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.10950456140202 #Duplication Level Percentage of deduplicated Percentage of total 1 76.08102540047679 40.40625565542765 2 13.273313272726314 14.098781836055524 3 4.403336772934048 7.015771032825903 4 1.9847673535818802 4.216400432735148 5 1.065440331893008 2.829250408328669 6 0.6648920024812779 2.118725090317149 7 0.45452621545378796 1.6897763479043826 8 0.3291615864053564 1.3985287019706882 9 0.22040576487363994 1.0535076877424294 >10 1.3422630997365408 13.207880178515238 >50 0.1158462633982976 4.275891065448889 >100 0.06169196540698115 5.712694215497486 >500 0.0027322835954533076 0.9429897477611375 >1k 4.269193117895616E-4 0.4210917430973616 >5k 1.7076772471582465E-4 0.6124558563722771 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7432 0.33540600282874705 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6074 0.2741194915476063 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3898 0.17591665756545427 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.2564989426045956E-5 0.0 22-23 0.0 0.0 0.0 1.579549259823217E-4 0.0 24-25 4.512997885209191E-5 0.0 0.0 2.7077987311255146E-4 0.0 26-27 4.512997885209191E-5 0.0 0.0 4.96429767373011E-4 0.0 28-29 1.1282494713022978E-4 0.0 0.0 0.0011056844818762518 0.0 30-31 1.8051991540836765E-4 0.0 0.0 0.002617538773421331 0.0 32-33 1.8051991540836765E-4 0.0 0.0 0.004648387821765467 0.0 34-35 1.8051991540836765E-4 0.0 0.0 0.0064535869758491436 0.0 36-37 2.2564989426045954E-4 0.0 0.0 0.009409600590661163 0.0 38-39 2.7077987311255146E-4 0.0 0.0 0.012027139364082494 0.0 40-41 2.933448625385974E-4 0.0 0.0 0.01599857750306658 0.0 42-43 3.836048202427812E-4 0.0 0.0 0.021910604732690624 0.0 44-45 4.512997885209191E-4 0.0 0.0 0.0318166350907248 0.0 46-47 5.866897250771948E-4 0.0 0.0 0.05668325343822744 0.0 48-49 6.318197039292868E-4 0.0 0.0 0.10341534653956862 0.0 50-51 6.318197039292868E-4 0.0 0.0 0.1372402656892115 0.0 52-53 6.318197039292868E-4 0.0 0.0 0.16907946576936234 0.0 54-55 6.318197039292868E-4 0.0 0.0 0.21184012073171943 0.0 56-57 6.769496827813787E-4 0.0 0.0 0.24692867928922088 0.0 58-59 6.769496827813787E-4 0.0 0.0 0.2944279820310476 0.0 60-61 6.769496827813787E-4 0.0 0.0 0.3699981316188755 0.0 62-63 6.769496827813787E-4 0.0 0.0 0.42927635884109827 4.512997885209191E-5 64-65 6.769496827813787E-4 0.0 0.0 0.499363215998397 4.512997885209191E-5 66-67 6.769496827813787E-4 0.0 0.0 0.6189802249458667 4.512997885209191E-5 68-69 6.769496827813787E-4 0.0 0.0 0.7516397977815907 4.512997885209191E-5 70-71 6.769496827813787E-4 0.0 0.0 0.870557292056853 4.512997885209191E-5 72-73 7.220796616334706E-4 0.0 0.0 1.0209078166025973 4.512997885209191E-5 74-75 7.672096404855625E-4 0.0 0.0 1.1560044082963343 4.512997885209191E-5 76-77 7.672096404855625E-4 0.0 0.0 1.2754408973283955 4.512997885209191E-5 78-79 7.672096404855625E-4 0.0 0.0 1.427912530880188 6.769496827813786E-5 80-81 8.349046087637003E-4 0.0 0.0 1.6188349064139627 9.025995770418382E-5 82-83 8.574695981897462E-4 0.0 0.0 1.81584982909277 1.3538993655627573E-4 84-85 8.800345876157922E-4 0.0 0.0 2.0497133795043103 1.579549259823217E-4 86-87 9.025995770418382E-4 0.0 0.0 2.417612967106564 1.8051991540836765E-4 88-89 9.025995770418382E-4 0.0 0.0 2.9355471694025965 1.8051991540836765E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4075 0.0 45.471195 1 GTATCAA 6940 0.0 38.611706 1 ATCAACG 8895 0.0 30.010422 3 TCAACGC 9030 0.0 29.561762 4 TATCAAC 9090 0.0 29.523397 2 CAACGCA 9350 0.0 28.550022 5 AACGCAG 9485 0.0 28.243187 6 ACGCAGA 10980 0.0 24.185219 7 GTACATG 7170 0.0 24.053974 1 TACCGTA 100 0.0016530883 23.752567 7 CGCAGAG 11190 0.0 23.688887 8 TACATGG 7435 0.0 22.807043 2 ACATGGG 7510 0.0 21.630568 3 GCAGAGT 12075 0.0 21.519924 9 TGGTATC 2315 0.0 20.928156 2 GAGTACT 6965 0.0 20.598063 12-13 AGAGTAC 10855 0.0 20.15303 10-11 TACACCG 240 1.4150646E-7 19.791128 5 AGTACTT 7460 0.0 19.64522 12-13 CATGGGG 4100 0.0 19.231182 4 >>END_MODULE