FastQCFastQC Report
Thu 26 May 2016
SRR937446_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937446_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214267
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74460.33627380979800536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54890.24789241767140097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35800.16167878580135095No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30950.13977537487574895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36600.052.097381
GTATCAA67150.042.1331181
ATCAACG86300.032.4964943
TCAACGC86650.032.480094
TATCAAC87800.031.8337552
CAACGCA89850.031.1638775
AACGCAG91250.030.5045836
ACGCAGA105800.026.0855887
CGCAGAG106600.025.622468
GTACATG73000.024.8173121
TACATGG73900.023.22032
GCAGAGT115200.023.174159
TGGTATC19400.022.2969112
ACATGGG74500.021.9481243
GAGTACT64600.021.029512-13
AGAGTAC105050.020.89108510-11
GTGGTAT24200.020.8277871
AGTACTT69700.020.68337212-13
CAGAGTA110800.019.46395910-11
GTACTTT71350.019.105314-15
Produced by FastQC (version 0.11.5)