FastQCFastQC Report
Thu 26 May 2016
SRR937446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937446_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214267
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77530.3501384431055514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63730.28781533572961165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40970.1850273702313226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41050.050.371531
GTATCAA74200.040.7438661
ATCAACG92550.032.028933
TCAACGC92950.031.89114
CAACGCA94850.031.2021855
TATCAAC95900.031.0586972
AACGCAG97650.030.303396
ACGCAGA114100.025.6882087
CGCAGAG114000.025.5857328
TAGATCG1000.001653226823.7522245
AACGCGA901.9544314E-723.74900676-77
GCAGAGT125350.022.9658539
GTACATG71050.022.0779551
GAGTACT73800.022.04644212-13
GACGCGT1302.9408376E-421.925138
TACATGG71850.020.9588182
AGAGTAC113100.020.77007110-11
GCCGCGC1150.00372233220.6541088
TGGTATC21300.020.0723042
GTACTTT81750.019.69642314-15
Produced by FastQC (version 0.11.5)