##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937446_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2214267 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25637739260893 33.0 31.0 34.0 31.0 34.0 2 32.491021182179026 34.0 31.0 34.0 31.0 34.0 3 32.57611254649959 34.0 31.0 34.0 31.0 34.0 4 36.16305666841442 37.0 35.0 37.0 35.0 37.0 5 36.0287038554971 37.0 35.0 37.0 35.0 37.0 6 36.10844852946822 37.0 35.0 37.0 35.0 37.0 7 36.071037503607286 37.0 35.0 37.0 35.0 37.0 8 36.046554909593105 37.0 35.0 37.0 35.0 37.0 9 37.763317160938584 39.0 37.0 39.0 35.0 39.0 10-11 37.68787458784329 39.0 37.0 39.0 35.0 39.0 12-13 37.602278541838004 39.0 37.0 39.0 35.0 39.0 14-15 38.87160243096248 40.0 38.0 41.0 35.0 41.0 16-17 38.85067089921857 40.0 38.0 41.0 35.0 41.0 18-19 38.8286841650081 40.0 38.0 41.0 35.0 41.0 20-21 38.73231073759398 40.0 38.0 41.0 34.5 41.0 22-23 38.6036087788871 40.0 38.0 41.0 34.0 41.0 24-25 38.462845944052816 40.0 38.0 41.0 34.0 41.0 26-27 38.26904298352457 40.0 38.0 41.0 34.0 41.0 28-29 38.26328735423506 40.0 38.0 41.0 34.0 41.0 30-31 38.41992880714024 40.0 38.0 41.0 34.0 41.0 32-33 38.4164244872005 40.0 38.0 41.0 34.0 41.0 34-35 38.32618785358767 40.0 38.0 41.0 34.0 41.0 36-37 38.19603620520922 40.0 38.0 41.0 34.0 41.0 38-39 38.079365541734575 40.0 38.0 41.0 33.5 41.0 40-41 37.93527113035601 40.0 38.0 41.0 33.0 41.0 42-43 37.75606216413829 40.0 37.0 41.0 33.0 41.0 44-45 37.52072265901086 40.0 37.0 41.0 33.0 41.0 46-47 37.24029532120562 40.0 37.0 41.0 32.0 41.0 48-49 36.974713528224015 40.0 37.0 41.0 31.0 41.0 50-51 36.7822338046857 40.0 36.0 41.0 31.0 41.0 52-53 36.55757638983917 39.0 36.0 41.0 30.5 41.0 54-55 36.36231109437118 39.0 36.0 41.0 30.0 41.0 56-57 36.1296476441188 39.0 35.0 40.0 30.0 41.0 58-59 35.88264807270306 39.0 35.0 40.0 29.0 41.0 60-61 35.558798013067076 38.0 35.0 40.0 28.5 41.0 62-63 35.197577347266616 38.0 34.0 40.0 28.0 41.0 64-65 34.853120468308475 37.5 34.0 40.0 28.0 41.0 66-67 34.482582046338585 37.0 34.0 39.5 27.5 41.0 68-69 34.05397135937084 36.0 33.5 39.0 26.0 40.5 70-71 33.66692950759777 36.0 33.0 39.0 26.0 40.0 72-73 33.23068333674304 35.0 33.0 38.0 26.0 40.0 74-75 32.78702771616973 35.0 32.0 37.0 25.5 39.0 76-77 31.478328494260175 34.0 30.5 36.0 24.0 38.5 78-79 32.085659949771184 35.0 32.0 36.0 25.0 39.0 80-81 31.90241059456696 35.0 32.0 36.0 25.0 37.5 82-83 31.57511040899765 35.0 32.0 35.5 24.0 37.0 84-85 31.24379286689455 34.0 31.5 35.0 23.0 37.0 86-87 30.90658375886919 34.0 31.0 35.0 21.5 36.0 88-89 30.568727032467176 34.0 31.0 35.0 20.0 36.0 90-91 30.144177283046716 34.0 30.5 35.0 18.0 35.5 92-93 29.743640897868232 34.0 30.0 35.0 14.0 35.0 94-95 29.3707441333859 34.0 30.0 35.0 4.5 35.0 96-97 28.96298346134409 34.0 29.5 35.0 2.0 35.0 98-99 28.344645654747147 34.0 29.0 35.0 2.0 35.0 100-101 26.751991291023167 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13996609449056052 1101 2 0.15286534969685306 1101 3 0.14703130034433087 1101 4 0.1212784383989387 1101 5 0.010633676772989986 1101 6 0.051720182363645506 1101 7 0.08892705115430033 1101 8 0.04608963525691934 1101 9 0.05315972358270926 1101 10-11 0.07906974937625222 1101 12-13 0.16548364555985984 1101 14-15 0.15270535393239015 1101 16-17 0.1493319264575277 1101 18-19 0.252710660776593 1101 20-21 0.07848612091878948 1101 22-23 0.0856500385839638 1101 24-25 0.08855607992859404 1101 26-27 0.05943706522711523 1101 28-29 0.10213180492500129 1101 30-31 0.12579482656715157 1101 32-33 0.10569495885017233 1101 34-35 0.12176584376581445 1101 36-37 0.1321647237690584 1101 38-39 0.10527632168962242 1101 40-41 0.03327677400357487 1101 42-43 0.07176583430317862 1101 44-45 0.09386077398833237 1101 46-47 0.12038070375872678 1101 48-49 0.15397620097285625 1101 50-51 0.15117280958363466 1101 52-53 0.31566768883981666 1101 54-55 0.2746602232979427 1101 56-57 0.20153886241654106 1101 58-59 0.35450017919783505 1101 60-61 0.27354114362304216 1101 62-63 0.27318621794948683 1101 64-65 0.26288532222698535 1101 66-67 0.19470705413483103 1101 68-69 0.13616436633719786 1101 70-71 0.08645124086965694 1101 72-73 0.023473858611517073 1101 74-75 0.12122226292901672 1101 76-77 0.1962658587379451 1101 78-79 0.2184835659945037 1101 80-81 0.049736349346794384 1101 82-83 0.016019119919704394 1101 84-85 0.10020102340683579 1101 86-87 -0.10725652478266667 1101 88-89 0.08227064249098959 1101 90-91 0.11655102482733426 1101 92-93 0.2778585996173426 1101 94-95 0.17220462718223217 1101 96-97 0.16517039118805954 1101 98-99 0.025617868169440783 1101 100-101 0.2212959002772834 1102 1 0.14402403116304896 1102 2 0.14458656648545798 1102 3 0.02301986703370318 1102 4 0.08107672636434415 1102 5 -0.016224236203520093 1102 6 0.07556426281149697 1102 7 0.06983697680816192 1102 8 0.1190362304407131 1102 9 0.13970929261206066 1102 10-11 0.08636366839524356 1102 12-13 0.1074692206316854 1102 14-15 0.11141510779039265 1102 16-17 0.11298451376240592 1102 18-19 0.16422098333813295 1102 20-21 0.12722949344922796 1102 22-23 0.19959438264960738 1102 24-25 0.18291421505492877 1102 26-27 0.18308195133830907 1102 28-29 0.09834044803391606 1102 30-31 0.00968822427456928 1102 32-33 0.029623456228804912 1102 34-35 0.09993592166635779 1102 36-37 0.1308503373468568 1102 38-39 0.13099016310427203 1102 40-41 -0.009805480668973132 1102 42-43 0.0955617822904955 1102 44-45 0.01187268121856988 1102 46-47 -0.019199729285311662 1102 48-49 0.09130403486403793 1102 50-51 0.028720127494828773 1102 52-53 0.028153989601044316 1102 54-55 -0.04115906523023938 1102 56-57 -0.015608882022306148 1102 58-59 -0.01091035366552262 1102 60-61 -0.03352914457932599 1102 62-63 0.11171292035758995 1102 64-65 -0.007130376360144908 1102 66-67 -0.0661856919331143 1102 68-69 -0.18120997091585167 1102 70-71 -0.1366056887236553 1102 72-73 -0.07417579631576388 1102 74-75 -0.22697165905196215 1102 76-77 -0.32685253230497224 1102 78-79 -0.2680282375658223 1102 80-81 -0.14140135392029052 1102 82-83 -0.17150864880752792 1102 84-85 -0.1264140057688401 1102 86-87 -0.06880609381201808 1102 88-89 -0.12868282452716784 1102 90-91 -0.2514586609047278 1102 92-93 -0.39613284020967754 1102 94-95 -0.5641479690086371 1102 96-97 -0.3394318125381304 1102 98-99 -0.11953852530231757 1102 100-101 -0.06540695212637715 1103 1 0.16404407838927426 1103 2 0.12299448622697184 1103 3 0.08547937033215192 1103 4 0.11378936394441297 1103 5 -5.158646384018084E-4 1103 6 0.12321121419805792 1103 7 0.11794270961907927 1103 8 0.1095518485780147 1103 9 0.07300116891936881 1103 10-11 0.13292454716822988 1103 12-13 0.13334905903625582 1103 14-15 0.14592753837523986 1103 16-17 0.08750746805386456 1103 18-19 0.20189596170364865 1103 20-21 0.17584401703406627 1103 22-23 0.25547385141985046 1103 24-25 0.24558843240860284 1103 26-27 0.2161299777084622 1103 28-29 0.19664904485853896 1103 30-31 0.21741727796721477 1103 32-33 0.23821734552115004 1103 34-35 0.22303067416356015 1103 36-37 0.1833019913517404 1103 38-39 0.09932011174684163 1103 40-41 0.20930994090984711 1103 42-43 0.1313249901945852 1103 44-45 0.02354823360445124 1103 46-47 0.007201945810457744 1103 48-49 0.01650472455819596 1103 50-51 0.059212784817724184 1103 52-53 0.15030182593566366 1103 54-55 0.06673936795509405 1103 56-57 0.12389002429496543 1103 58-59 0.006779366586521007 1103 60-61 0.0019530325233532153 1103 62-63 0.04991984600021482 1103 64-65 0.03879016530634516 1103 66-67 -0.016810156506927854 1103 68-69 0.004760667072993385 1103 70-71 0.018467324926717765 1103 72-73 0.03413793190298975 1103 74-75 -0.03318869093184418 1103 76-77 -0.14104007606314894 1103 78-79 0.08749964373372165 1103 80-81 -0.04917106738846044 1103 82-83 -0.06626995200769059 1103 84-85 -0.1200986946454563 1103 86-87 0.11453470468509508 1103 88-89 -0.01679723287307766 1103 90-91 -0.011849190438507406 1103 92-93 0.02969242277427142 1103 94-95 -0.037431017578587245 1103 96-97 -0.033457217287715224 1103 98-99 0.07800003625484209 1103 100-101 0.36682929204100745 1104 1 0.026134410517869355 1104 2 0.09433921280174928 1104 3 0.13417583879049744 1104 4 0.1040249257767698 1104 5 -0.02553057715023499 1104 6 0.031857591268213525 1104 7 0.03893023496560488 1104 8 0.07407972811442676 1104 9 0.11389806040359218 1104 10-11 0.1351210219127026 1104 12-13 0.12474568201340475 1104 14-15 0.08459896413288703 1104 16-17 0.10875260454346858 1104 18-19 0.11378066290645705 1104 20-21 0.0675436908144249 1104 22-23 0.07796387894107681 1104 24-25 0.04888633996912972 1104 26-27 0.06670548686913236 1104 28-29 -0.06805388812485091 1104 30-31 0.08928611719642987 1104 32-33 -0.014964628044822348 1104 34-35 -0.026330237072244245 1104 36-37 -0.027294818772148233 1104 38-39 -0.011166362021782561 1104 40-41 -0.0406326608369838 1104 42-43 0.042451989554599834 1104 44-45 0.16390791635300417 1104 46-47 -0.024113553304395907 1104 48-49 -0.03963548163847719 1104 50-51 -0.01000681446314644 1104 52-53 0.04061548531303316 1104 54-55 0.09931538359735015 1104 56-57 -0.021711084336956787 1104 58-59 -0.05340341280003713 1104 60-61 -0.32122801907849663 1104 62-63 -0.11236125487284454 1104 64-65 -0.09170562389194714 1104 66-67 -0.18249773519566048 1104 68-69 -0.12703500173115145 1104 70-71 -0.2620284343376724 1104 72-73 -0.16479505926591287 1104 74-75 -0.2176549275880575 1104 76-77 -0.14317728168569488 1104 78-79 -0.12100568479980467 1104 80-81 -0.07005976596426677 1104 82-83 -0.09643419333000125 1104 84-85 -0.2529906925357146 1104 86-87 -0.14899683995802704 1104 88-89 -0.06830441970311796 1104 90-91 0.04696471170278471 1104 92-93 -0.05176467865435441 1104 94-95 -0.11849957698570535 1104 96-97 -8.286453346784128E-5 1104 98-99 0.05337274324326913 1104 100-101 0.5151067906685434 1105 1 0.03495046958580872 1105 2 0.08882166646429823 1105 3 0.10243409814160742 1105 4 0.06054538403623866 1105 5 -0.021939774687837144 1105 6 0.009314456870967547 1105 7 0.07645552871390038 1105 8 -0.012026891785886562 1105 9 -0.044829127567005855 1105 10-11 0.05698471265399263 1105 12-13 -0.029674781387136306 1105 14-15 0.08799437726361958 1105 16-17 0.09052074369186158 1105 18-19 0.03199073199889568 1105 20-21 0.043168474557568004 1105 22-23 -0.018159151930149164 1105 24-25 -0.006485956856295161 1105 26-27 0.01814481162207926 1105 28-29 -0.09223089901755088 1105 30-31 -0.14590776158651408 1105 32-33 -0.0057460929478168055 1105 34-35 0.02901115560167966 1105 36-37 -0.024338273475038363 1105 38-39 -0.04760134575233366 1105 40-41 -0.003405938739241776 1105 42-43 -0.1943348971496235 1105 44-45 -0.15327200472876967 1105 46-47 -0.2110924789836659 1105 48-49 -0.18671433170366925 1105 50-51 -0.21136425765583056 1105 52-53 -0.13441075433335925 1105 54-55 -0.15282133521356656 1105 56-57 -0.1484563024249539 1105 58-59 -0.1418643080730888 1105 60-61 -0.2080887869224668 1105 62-63 -0.197215823401784 1105 64-65 -0.1604045417845299 1105 66-67 -0.16938412171474937 1105 68-69 -0.06494047430761896 1105 70-71 -0.17091967425266574 1105 72-73 -0.11540062178212196 1105 74-75 -0.121641721984183 1105 76-77 -0.23652204377860642 1105 78-79 -0.11866635606108034 1105 80-81 -0.16848696127099316 1105 82-83 -0.14483745958110816 1105 84-85 -0.3182552060783763 1105 86-87 -0.18874383893385627 1105 88-89 -0.03805712583814014 1105 90-91 -0.2826321797439313 1105 92-93 -0.18333426584998236 1105 94-95 0.005290106688640606 1105 96-97 -0.1631203151662497 1105 98-99 -0.11856170934023069 1105 100-101 -0.10448093928578572 1106 1 -0.12361075700673751 1106 2 -0.002574469293975312 1106 3 -0.08346237795237954 1106 4 0.02413819004672746 1106 5 -0.10074073356799573 1106 6 -0.026780265154521032 1106 7 -0.00330213634126153 1106 8 -0.0346129042486325 1106 9 -0.042172120739166985 1106 10-11 -0.06769933241423587 1106 12-13 -0.2936838135262718 1106 14-15 -0.04216305594071201 1106 16-17 0.012122507742311939 1106 18-19 0.012639133523379087 1106 20-21 -0.22296639269007557 1106 22-23 -0.18084819287545884 1106 24-25 -0.08167511645215342 1106 26-27 -0.037778254442848436 1106 28-29 -0.038149136387851 1106 30-31 -0.10331075978639603 1106 32-33 -0.07396219800698134 1106 34-35 -0.04953206366484153 1106 36-37 -0.020294356543281822 1106 38-39 -0.10600971981725849 1106 40-41 -0.045251461541120364 1106 42-43 -0.13873050349680227 1106 44-45 0.0325264889757122 1106 46-47 0.014704875675541018 1106 48-49 -0.15438971176051552 1106 50-51 -0.11370804234197607 1106 52-53 0.024030750027016268 1106 54-55 -0.04726697381838818 1106 56-57 0.025532557589748706 1106 58-59 0.00530769051963631 1106 60-61 -0.014343538685615442 1106 62-63 -0.09448021632587 1106 64-65 -0.06846376172834567 1106 66-67 0.002773845493607041 1106 68-69 0.24756540080831968 1106 70-71 0.02012194897259434 1106 72-73 0.0746532930277013 1106 74-75 0.06973541437637465 1106 76-77 -0.06231103931520465 1106 78-79 0.041889347999031656 1106 80-81 -0.0364530887589396 1106 82-83 -0.07462542262827654 1106 84-85 -0.10786902686185229 1106 86-87 -0.03134822003268667 1106 88-89 0.016421312371562635 1106 90-91 0.042239149475982174 1106 92-93 -0.09305757889177713 1106 94-95 -0.24631389564701323 1106 96-97 0.1442753955934073 1106 98-99 0.051657431335843995 1106 100-101 -0.04912800824977026 1107 1 0.03822887658331098 1107 2 -0.004718306251284332 1107 3 0.05798697651373175 1107 4 0.030382505176014263 1107 5 0.005137487030182797 1107 6 0.029337829988229203 1107 7 -0.015451688597160285 1107 8 -0.027955969932051516 1107 9 -0.0012021382279101545 1107 10-11 0.0037846704067305836 1107 12-13 -0.046101587110619846 1107 14-15 0.030255039922025162 1107 16-17 -0.03012758851264863 1107 18-19 -0.03882109612396789 1107 20-21 0.05568732707104829 1107 22-23 -0.005064704979922396 1107 24-25 0.03805070349776685 1107 26-27 0.007876507237426722 1107 28-29 0.0014634198522998076 1107 30-31 0.029744528441902673 1107 32-33 0.03422254156294002 1107 34-35 0.013027227843835476 1107 36-37 0.09116048008386457 1107 38-39 0.2013332903245768 1107 40-41 0.04519922420900002 1107 42-43 -0.0034510658520119364 1107 44-45 -0.004142277071785827 1107 46-47 0.030391261779442402 1107 48-49 -0.06889328476497525 1107 50-51 -0.030585161365216607 1107 52-53 0.13787781796226284 1107 54-55 0.13000285170215164 1107 56-57 0.18023938197168832 1107 58-59 0.08131876181070652 1107 60-61 -0.08753750261718096 1107 62-63 0.00920513985860083 1107 64-65 0.008955306112277128 1107 66-67 -0.056630460625605394 1107 68-69 -0.09670925511665729 1107 70-71 -0.1591687163025668 1107 72-73 0.040395700714661587 1107 74-75 -0.0185977481036943 1107 76-77 -0.005183767124020022 1107 78-79 0.10047604414160105 1107 80-81 0.1747345653732637 1107 82-83 0.09122229123514458 1107 84-85 0.05037883127257459 1107 86-87 0.062337849278424784 1107 88-89 0.2622879206624127 1107 90-91 0.18081936919441333 1107 92-93 0.16128143671232564 1107 94-95 0.1350289458129339 1107 96-97 0.19387181048637459 1107 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-0.3492376859738826 2316 58-59 -0.3618885595600503 2316 60-61 -0.3062462809993889 2316 62-63 -0.22584184406508 2316 64-65 -0.22618773860339303 2316 66-67 -0.1366982934977159 2316 68-69 -0.26143007955795383 2316 70-71 -0.19444824962958052 2316 72-73 -0.20236888236897954 2316 74-75 -0.21424102447435445 2316 76-77 -0.08797833119227505 2316 78-79 0.05216891435578219 2316 80-81 0.11774225202546518 2316 82-83 0.07219992892675364 2316 84-85 -0.05654970987346175 2316 86-87 -0.19823930256381672 2316 88-89 -0.2154209668391296 2316 90-91 -0.044715986364682436 2316 92-93 -0.18635899182255855 2316 94-95 -0.18361060440074084 2316 96-97 -0.27927535214258015 2316 98-99 -0.5295020375820307 2316 100-101 -0.7521127448393408 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 11.0 5 47.0 6 64.0 7 160.0 8 407.0 9 876.0 10 1385.0 11 2293.0 12 3390.0 13 4909.0 14 6550.0 15 8778.0 16 12125.0 17 17262.0 18 15674.0 19 9611.0 20 8204.0 21 8652.0 22 8999.0 23 9643.0 24 11130.0 25 13593.0 26 16959.0 27 21173.0 28 26567.0 29 33965.0 30 43568.0 31 55173.0 32 72127.0 33 96203.0 34 134315.0 35 196780.0 36 317751.0 37 526731.0 38 479777.0 39 49407.0 40 3.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.35807781337784 19.86840577326519 15.05051358528299 22.723002828073977 2 15.480201800415216 20.862705355767847 39.58605714667653 24.07103569714041 3 19.343195739267216 24.575401250165406 29.943091777098246 26.138311233469135 4 13.329964272601272 17.323656090254698 36.82049183770521 32.52588779943882 5 14.046341463414635 37.331842818428186 34.19241192411924 14.42940379403794 6 32.300079439381065 36.63352251557739 17.101912280677986 13.964485764363557 7 28.758591443579295 32.46076466839817 20.559218919850224 18.22142496817231 8 26.503759483386602 35.67790153581298 19.520816595288647 18.297522385511776 9 26.047762081085974 17.064879709628514 20.3440687143872 36.54328949489831 10-11 24.707115266587092 27.137377741708658 27.393670230374205 20.761836761330045 12-13 25.67012197338865 24.221765609569985 28.139691856464648 21.968420560576714 14-15 22.485702943682945 25.849886215167366 26.15389201031312 25.51051883083657 16-17 22.122783747398124 29.36285461509384 26.57287942240028 21.941482215107754 18-19 22.096906109335503 27.631559337695048 28.95777699798624 21.313757554983205 20-21 22.566858468287702 26.8419526642451 29.359151357988893 21.232037509478303 22-23 22.504782113776713 26.806640922700133 29.029186305642952 21.659390657880206 24-25 22.369610413598952 26.983321956311784 29.139280711804876 21.50778691828438 26-27 22.33649831905296 27.299672843085716 28.90657601656198 21.457252821299342 28-29 22.047347932719592 27.250724914874297 29.140618243263837 21.56130890914227 30-31 22.183126976825793 26.947605973217577 29.211735528568244 21.65753152138839 32-33 21.970498589375175 27.199316974872495 29.13738496757618 21.692799468176148 34-35 22.462873718481106 27.03723173402304 28.92442510320571 21.575469444290142 36-37 22.37526543832663 26.946020841255464 28.744204615352654 21.934509105065253 38-39 22.187818955316903 27.197619069124674 28.95418785508477 21.66037412047366 40-41 22.420331423446225 27.076138514460997 28.89306032199369 21.610469740099095 42-43 22.304644238603945 27.165232166763325 28.684510291723804 21.84561330290893 44-45 22.311158119404325 27.39051509834069 28.48062778351206 21.817698998742923 46-47 22.413466849300466 27.54778443611362 28.090718057036483 21.948030657549428 48-49 22.56704421080299 27.668552994863084 27.93292948251712 21.8314733118168 50-51 22.45808658124788 27.760179779583943 27.83765462791976 21.94407901124842 52-53 22.712587989109707 27.843950962520076 27.552319267946867 21.89114178042335 54-55 22.44948328272968 27.723463340238553 27.76379271334487 22.063260663686897 56-57 22.418344309877718 28.11585052751091 27.732631159656897 21.733174002954478 58-59 22.687846587606643 27.711066461271383 27.635804534864132 21.965282416257843 60-61 22.546693781734543 27.659424089326173 28.033453056925833 21.760429072013448 62-63 22.45871884465604 27.81873188734692 28.009359304907672 21.713189963089366 64-65 22.433857344213685 27.679159739995224 27.93317156422419 21.953811351566905 66-67 22.3146756917752 27.955707238557952 27.755234576498676 21.974382493168168 68-69 22.34649208970734 27.714363263328224 27.89329380783799 22.045850839126444 70-71 22.273330181048625 27.806989852623914 27.833951370814813 22.085728595512645 72-73 22.3846537025571 27.826725503292966 27.641088450489487 22.147532343660455 74-75 22.36107930976707 27.99863340780493 27.497158201788675 22.143129080639326 76-77 22.43993158909924 27.91194557837876 27.585765402275335 22.062357430246667 78-79 22.40506677830632 27.92883604371108 27.457325607074484 22.208771570908116 80-81 22.290694843937068 28.233632168117033 27.470512815301856 22.00516017264404 82-83 22.345699864875606 28.10646104154172 27.47624935870107 22.071589734881602 84-85 22.35800592973289 27.963500303712518 27.34903003931327 22.32946372724132 86-87 22.324069319553605 27.84840310585851 27.592765461437125 22.234762113150765 88-89 22.388650510530123 27.736989261006013 27.483316149317133 22.39104407914673 90-91 22.27324410901581 27.82500200421619 27.58670916734133 22.31504471942667 92-93 22.17418902773202 27.728067285412695 27.557554964875724 22.54018872197956 94-95 22.1363774106736 27.550923172318427 27.636210989912236 22.67648842709574 96-97 22.11124494019917 27.774496029611605 27.530826228273288 22.58343280191594 98-99 21.974499064574292 27.857192051873504 27.310433750746594 22.85787513280561 100-101 21.78141060370337 28.258528249314196 27.331996542923598 22.628064604058835 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 226.0 1 170.5 2 151.0 3 212.0 4 200.5 5 206.0 6 365.0 7 782.0 8 1358.0 9 1783.0 10 1856.5 11 1867.5 12 2087.5 13 2322.0 14 2570.5 15 2785.0 16 2871.5 17 3018.5 18 3200.5 19 3338.0 20 3677.5 21 4175.5 22 4784.5 23 5816.0 24 7112.0 25 8569.5 26 10903.5 27 14282.0 28 17529.0 29 21233.0 30 26311.0 31 30513.0 32 34692.5 33 39296.5 34 44560.5 35 49728.5 36 54602.0 37 59746.0 38 63066.0 39 65080.5 40 67669.5 41 72885.0 42 78539.5 43 82775.5 44 87131.5 45 89121.5 46 87933.5 47 88502.5 48 92414.5 49 92247.5 50 88335.0 51 83519.5 52 77422.0 53 72177.0 54 67746.5 55 62770.5 56 55816.5 57 49371.5 58 44210.0 59 39399.0 60 33639.0 61 26533.0 62 20033.0 63 15193.0 64 11399.0 65 8683.0 66 6526.0 67 4992.5 68 3477.0 69 2036.5 70 1399.5 71 1027.0 72 783.0 73 551.0 74 393.5 75 278.0 76 165.0 77 89.0 78 49.5 79 29.5 80 21.0 81 16.0 82 11.0 83 8.5 84 4.5 85 1.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0496778392127056 2 0.0 3 0.0 4 0.0 5 0.012058166427083997 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00616456822957665 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011967843083060896 24-25 3.8387421209817965E-4 26-27 0.0017613052084504714 28-29 0.0018742093884793478 30-31 7.45167588190584E-4 32-33 0.0 34-35 0.0 36-37 2.2580836005775277E-4 38-39 3.161317040808538E-4 40-41 0.0 42-43 0.01151622636294539 44-45 2.2580836005775275E-5 46-47 0.0 48-49 2.2580836005775275E-5 50-51 0.0 52-53 6.774250801732583E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.967783921270561E-4 84-85 9.03233440231011E-5 86-87 0.0 88-89 0.0 90-91 0.008332328486131076 92-93 0.0025967961406641563 94-95 0.0 96-97 0.0 98-99 0.001558077684398494 100-101 0.011629130542974268 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2214267.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.11633622378971 #Duplication Level Percentage of deduplicated Percentage of total 1 76.0827656283371 40.41237759950544 2 13.38698641941619 14.221353433540337 3 4.3369802747088935 6.9109350740214435 4 1.9659713364487357 4.177007780525769 5 1.0591209986761096 2.8128313533678084 6 0.6563168351627789 2.0916687411503836 7 0.44353812177396124 1.6491384002949732 8 0.315907315784161 1.342387136059712 9 0.24163400429384582 1.1551241713655303 >10 1.32680942239841 13.06330195483233 >50 0.1185197007957605 4.35819456207407 >100 0.06194919120479143 5.723662229873423 >500 0.002903061804251863 1.0050665742101106 >1k 4.2692085356643353E-4 0.43629531356441315 >5k 1.7076834142657342E-4 0.6406556756144387 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7753 0.3501384431055514 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6373 0.28781533572961165 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4097 0.1850273702313226 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.516167201155055E-5 2 4.516167201155055E-5 0.0 0.0 0.0 4.516167201155055E-5 3 4.516167201155055E-5 0.0 0.0 0.0 4.516167201155055E-5 4 4.516167201155055E-5 0.0 0.0 0.0 4.516167201155055E-5 5 4.516167201155055E-5 0.0 0.0 0.0 4.516167201155055E-5 6 4.516167201155055E-5 0.0 0.0 0.0 4.516167201155055E-5 7 4.516167201155055E-5 0.0 0.0 0.0 4.516167201155055E-5 8 4.516167201155055E-5 0.0 0.0 0.0 4.516167201155055E-5 9 4.516167201155055E-5 0.0 0.0 0.0 4.516167201155055E-5 10-11 4.516167201155055E-5 0.0 0.0 0.0 4.516167201155055E-5 12-13 4.516167201155055E-5 0.0 0.0 0.0 4.516167201155055E-5 14-15 4.516167201155055E-5 0.0 0.0 0.0 4.516167201155055E-5 16-17 6.774250801732583E-5 0.0 0.0 0.0 4.516167201155055E-5 18-19 1.3548501603465166E-4 0.0 0.0 0.0 4.516167201155055E-5 20-21 1.806466880462022E-4 0.0 0.0 2.2580836005775275E-5 6.774250801732583E-5 22-23 1.806466880462022E-4 0.0 0.0 1.1290418002887637E-4 9.03233440231011E-5 24-25 1.806466880462022E-4 0.0 0.0 1.806466880462022E-4 9.03233440231011E-5 26-27 1.806466880462022E-4 0.0 0.0 3.161317040808538E-4 9.03233440231011E-5 28-29 2.2580836005775274E-4 0.0 0.0 9.709759482483368E-4 9.03233440231011E-5 30-31 2.2580836005775274E-4 0.0 0.0 0.0021677602565544265 9.03233440231011E-5 32-33 2.2580836005775274E-4 0.0 0.0 0.004087131317045325 9.03233440231011E-5 34-35 2.2580836005775274E-4 0.0 0.0 0.006661346621703706 9.03233440231011E-5 36-37 2.2580836005775274E-4 0.0 0.0 0.00898717273029856 9.03233440231011E-5 38-39 3.161317040808538E-4 0.0 0.0 0.011719453886997367 9.03233440231011E-5 40-41 3.161317040808538E-4 0.0 0.0 0.01519690263188676 1.3548501603465166E-4 42-43 3.387125400866291E-4 0.0 0.0 0.021338890025457635 1.3548501603465166E-4 44-45 4.0645504810395497E-4 0.0 0.0 0.030551871115813947 1.3548501603465166E-4 46-47 4.290358841097302E-4 0.0 0.0 0.051890761141271585 1.3548501603465166E-4 48-49 4.516167201155055E-4 0.0 0.0 0.09267175096770172 1.3548501603465166E-4 50-51 4.516167201155055E-4 0.0 0.0 0.12299781372345792 1.3548501603465166E-4 52-53 4.516167201155055E-4 0.0 0.0 0.15158515210676943 1.3548501603465166E-4 54-55 4.516167201155055E-4 0.0 0.0 0.18780481306003297 1.3548501603465166E-4 56-57 4.516167201155055E-4 0.0 0.0 0.2163695706073387 1.3548501603465166E-4 58-59 4.516167201155055E-4 0.0 0.0 0.2578279855139421 1.3548501603465166E-4 60-61 4.516167201155055E-4 0.0 0.0 0.3253446851712102 1.3548501603465166E-4 62-63 4.516167201155055E-4 0.0 0.0 0.3740063867636559 1.3548501603465166E-4 64-65 4.516167201155055E-4 0.0 0.0 0.4344778655871221 1.806466880462022E-4 66-67 5.419400641386066E-4 0.0 0.0 0.5468626863878656 1.806466880462022E-4 68-69 5.419400641386066E-4 0.0 0.0 0.6619572075093022 1.806466880462022E-4 70-71 5.419400641386066E-4 0.0 0.0 0.7715645854813353 1.806466880462022E-4 72-73 5.419400641386066E-4 0.0 0.0 0.906959278171964 1.806466880462022E-4 74-75 5.419400641386066E-4 0.0 0.0 1.031740977939878 1.806466880462022E-4 76-77 5.419400641386066E-4 0.0 0.0 1.1403322182916513 1.806466880462022E-4 78-79 5.419400641386066E-4 0.0 0.0 1.2851431195966883 1.806466880462022E-4 80-81 5.871017361501571E-4 0.0 0.0 1.4630123648141802 1.806466880462022E-4 82-83 6.096825721559324E-4 0.0 0.0 1.6537526865549639 1.806466880462022E-4 84-85 7.000059161790335E-4 0.0 0.0 1.883038495357606 1.806466880462022E-4 86-87 7.677484241963593E-4 0.0 0.0 2.2481480327349863 1.806466880462022E-4 88-89 7.677484241963593E-4 0.0 0.0 2.765407243119281 1.806466880462022E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4105 0.0 50.37153 1 GTATCAA 7420 0.0 40.743866 1 ATCAACG 9255 0.0 32.02893 3 TCAACGC 9295 0.0 31.8911 4 CAACGCA 9485 0.0 31.202185 5 TATCAAC 9590 0.0 31.058697 2 AACGCAG 9765 0.0 30.30339 6 ACGCAGA 11410 0.0 25.688208 7 CGCAGAG 11400 0.0 25.585732 8 TAGATCG 100 0.0016532268 23.752224 5 AACGCGA 90 1.9544314E-7 23.749006 76-77 GCAGAGT 12535 0.0 22.965853 9 GTACATG 7105 0.0 22.077955 1 GAGTACT 7380 0.0 22.046442 12-13 GACGCGT 130 2.9408376E-4 21.92513 8 TACATGG 7185 0.0 20.958818 2 AGAGTAC 11310 0.0 20.770071 10-11 GCCGCGC 115 0.003722332 20.654108 8 TGGTATC 2130 0.0 20.072304 2 GTACTTT 8175 0.0 19.696423 14-15 >>END_MODULE