FastQCFastQC Report
Thu 26 May 2016
SRR937445_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937445_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2409473
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78130.3242617784054853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57790.23984497854925121No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37350.15501315017848302No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35660.1479991682828569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39950.051.5481951
GTATCAA75100.043.5070041
ATCAACG94750.034.0677223
TCAACGC95200.033.858164
TATCAAC97550.033.0905572
CAACGCA97550.033.0370065
AACGCAG99600.032.3375176
ACGCAGA113600.028.1036917
CGCAGAG114450.027.6868428
GTACATG82400.025.8578951
GCAGAGT121650.025.6571049
TACATGG81200.025.35092
ACATGGG83250.024.0408823
GAGTACT70000.022.22424312-13
AGAGTAC112700.021.83467110-11
CAGAGTA120550.020.62957610-11
AGTACTT77000.020.57400512-13
GTACTTT77200.019.9035314-15
CATGGGG48650.019.6419144
TGGTATC20400.019.1093222
Produced by FastQC (version 0.11.5)