##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937445_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2409473 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.601387938358304 33.0 31.0 34.0 30.0 34.0 2 31.70680684116402 33.0 31.0 34.0 30.0 34.0 3 31.75601760218936 33.0 31.0 34.0 30.0 34.0 4 35.3728894243679 37.0 35.0 37.0 33.0 37.0 5 35.35085514550277 37.0 35.0 37.0 33.0 37.0 6 35.401847623941 37.0 35.0 37.0 33.0 37.0 7 35.33871846665225 37.0 35.0 37.0 33.0 37.0 8 35.326856951706866 37.0 35.0 37.0 33.0 37.0 9 36.93219056615285 39.0 37.0 39.0 33.0 39.0 10-11 36.84013246880127 39.0 37.0 39.0 33.0 39.0 12-13 36.7275889790008 39.0 37.0 39.0 33.0 39.0 14-15 37.89362341889699 40.0 38.0 41.0 33.0 41.0 16-17 37.83134507006304 40.0 38.0 41.0 33.0 41.0 18-19 37.76639746533786 40.0 38.0 41.0 32.0 41.0 20-21 37.68552625408129 40.0 37.5 41.0 32.0 41.0 22-23 37.546714987053186 40.0 37.0 41.0 32.0 41.0 24-25 37.39942136724504 40.0 37.0 41.0 32.0 41.0 26-27 37.24040402195833 39.5 37.0 41.0 31.5 41.0 28-29 37.04945438276337 39.0 36.0 41.0 31.0 41.0 30-31 37.060427529173396 39.0 36.5 41.0 31.0 41.0 32-33 36.938893069148314 39.0 36.0 41.0 31.0 41.0 34-35 36.75089449020595 39.0 36.0 41.0 30.0 41.0 36-37 36.79030850314571 39.0 36.0 41.0 30.0 41.0 38-39 36.78292161812978 39.0 36.0 41.0 30.0 41.0 40-41 36.63729682797857 39.5 36.0 41.0 30.0 41.0 42-43 36.42272833104998 39.0 36.0 41.0 30.0 41.0 44-45 36.230320696683464 39.0 35.5 41.0 30.0 41.0 46-47 35.95375939053892 39.0 35.0 41.0 28.5 41.0 48-49 35.68610667145886 39.0 35.0 41.0 27.5 41.0 50-51 34.78977456896176 38.0 34.0 39.5 26.0 40.5 52-53 34.93599015220341 38.0 34.5 39.5 26.5 40.5 54-55 35.29302403471631 39.0 35.0 40.0 27.0 41.0 56-57 35.09079060026819 38.5 35.0 40.0 26.0 41.0 58-59 34.84416633844828 38.0 34.0 40.0 26.0 41.0 60-61 34.57075821144292 38.0 34.0 40.0 25.5 41.0 62-63 34.25328318682135 38.0 34.0 40.0 24.5 41.0 64-65 33.89247129974065 37.0 33.5 40.0 23.0 41.0 66-67 33.50562156122936 37.0 33.0 39.5 22.5 41.0 68-69 33.019099819753116 36.0 32.5 39.0 21.5 41.0 70-71 32.596031165321214 35.5 32.0 39.0 20.0 40.0 72-73 32.18118733847609 35.0 31.5 38.0 18.5 40.0 74-75 31.711985774482635 35.0 31.0 37.5 18.0 39.5 76-77 31.259519612794996 35.0 31.0 37.0 14.5 39.0 78-79 30.778096081591286 35.0 30.5 36.0 9.5 39.0 80-81 30.318315249849242 34.0 30.0 36.0 7.0 37.5 82-83 29.873872419404577 34.0 30.0 35.5 4.5 37.0 84-85 29.40340813115565 34.0 29.0 35.0 2.0 37.0 86-87 28.96561364248531 34.0 29.0 35.0 2.0 36.0 88-89 28.582572413137648 34.0 29.0 35.0 2.0 36.0 90-91 28.141586562704788 34.0 28.0 35.0 2.0 35.5 92-93 27.689007098232686 33.5 27.0 35.0 2.0 35.0 94-95 27.1601464303605 33.0 26.0 35.0 2.0 35.0 96-97 26.54320965621943 33.0 25.0 35.0 2.0 35.0 98-99 25.82175127092107 32.5 24.0 35.0 2.0 35.0 100-101 23.989051132758078 30.5 10.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19956568819661058 1101 2 0.1379968802184912 1101 3 0.26848535801011764 1101 4 0.20953373682176135 1101 5 0.24237452935653891 1101 6 0.07081840935320827 1101 7 0.2529723673339319 1101 8 0.1683952606482677 1101 9 0.24658571295026377 1101 10-11 0.25099966200765067 1101 12-13 0.2396328522686204 1101 14-15 0.38752288111888333 1101 16-17 0.2980653345079105 1101 18-19 0.27416571196153683 1101 20-21 0.39300491359819034 1101 22-23 0.2440562449749919 1101 24-25 0.37057348256926304 1101 26-27 0.37779747372966455 1101 28-29 0.3237561866962295 1101 30-31 0.35404578481607274 1101 32-33 0.2664929781259957 1101 34-35 0.2128228754479764 1101 36-37 0.3082424741606289 1101 38-39 0.4196778225641893 1101 40-41 0.35771707989474777 1101 42-43 0.5413492653910836 1101 44-45 0.3070840703218707 1101 46-47 0.4361153379712519 1101 48-49 0.5246082660988804 1101 50-51 0.5103517202543557 1101 52-53 0.5821200950717582 1101 54-55 0.5623662527264344 1101 56-57 0.5185507784788257 1101 58-59 0.5571674491329546 1101 60-61 0.5650905786500289 1101 62-63 0.5685725526686625 1101 64-65 0.5076011502669644 1101 66-67 0.6250832249878826 1101 68-69 0.5743800177220706 1101 70-71 0.4965989996286524 1101 72-73 0.561301016739499 1101 74-75 0.5374842620570597 1101 76-77 0.5476390074955759 1101 78-79 0.6127162001212341 1101 80-81 0.6932352021521773 1101 82-83 0.7684398205712171 1101 84-85 0.5973609508573468 1101 86-87 0.7659042541705112 1101 88-89 0.7043030430343116 1101 90-91 0.7859189466055483 1101 92-93 0.8019445229741251 1101 94-95 0.7812408565509585 1101 96-97 0.5869248716921582 1101 98-99 0.6806856622817712 1101 100-101 1.0435268042058077 1102 1 0.0765306072476406 1102 2 -0.03751628662275053 1102 3 0.11974139932073768 1102 4 -0.14028709122829497 1102 5 -0.005849371262748093 1102 6 -0.007250943973879487 1102 7 0.06799524621369102 1102 8 -0.023726686537351327 1102 9 0.12863727771862443 1102 10-11 0.08489807610617817 1102 12-13 0.22806385728544143 1102 14-15 0.2279606119750497 1102 16-17 0.17534081827217562 1102 18-19 0.17075133777699847 1102 20-21 0.3122305232422349 1102 22-23 0.2444761505192119 1102 24-25 0.2323014352311148 1102 26-27 0.30852500372948555 1102 28-29 0.26238841559528225 1102 30-31 0.21373485257063862 1102 32-33 0.1260644355749534 1102 34-35 0.20803852496926822 1102 36-37 0.2816175223992232 1102 38-39 0.3679834990990898 1102 40-41 0.2932023142884148 1102 42-43 0.33006878318882826 1102 44-45 0.2517723111289669 1102 46-47 0.22529518985577823 1102 48-49 0.3651204222265605 1102 50-51 0.26568369371853606 1102 52-53 0.27433208764760053 1102 54-55 0.2671193629677191 1102 56-57 0.28401193951306425 1102 58-59 0.24643734640770276 1102 60-61 0.27899248430450996 1102 62-63 0.38749954239614226 1102 64-65 0.3588737305268168 1102 66-67 0.4851361496254043 1102 68-69 0.4033378801547727 1102 70-71 0.1315457993419713 1102 72-73 0.01889698858652622 1102 74-75 0.10148540140690088 1102 76-77 0.1380032319107407 1102 78-79 0.1109456137236009 1102 80-81 0.0994685483491331 1102 82-83 0.2440908119893379 1102 84-85 0.309410494198584 1102 86-87 0.2563971148574318 1102 88-89 0.36403070167040497 1102 90-91 0.3500165631591976 1102 92-93 0.16128351630341697 1102 94-95 0.023609050313936564 1102 96-97 0.24617290080070475 1102 98-99 0.5256348509544289 1102 100-101 0.8393369370686976 1103 1 0.1140431911790003 1103 2 0.10210670341169603 1103 3 0.18448699609925256 1103 4 0.05911442161423963 1103 5 0.05075015031695784 1103 6 0.09973602415298899 1103 7 0.12186647911570958 1103 8 0.1825130962339756 1103 9 0.12954424436774303 1103 10-11 0.06876847777603956 1103 12-13 0.15550006021691587 1103 14-15 0.19874557221444178 1103 16-17 0.3253911539976144 1103 18-19 0.25358454642550754 1103 20-21 0.13324314873520393 1103 22-23 0.19957945420526357 1103 24-25 0.1625619803347007 1103 26-27 0.1809650395648319 1103 28-29 -0.026213544265438316 1103 30-31 0.011382465548940957 1103 32-33 0.174393197131387 1103 34-35 0.21623170332398445 1103 36-37 0.31348881071671997 1103 38-39 0.3591299529703278 1103 40-41 0.4179951989410924 1103 42-43 0.27339260353185324 1103 44-45 0.15817464596775466 1103 46-47 0.220085211375455 1103 48-49 0.05091651174102907 1103 50-51 0.05159061418808619 1103 52-53 0.27768657122152973 1103 54-55 0.32381863240700426 1103 56-57 0.32257834110902195 1103 58-59 0.24823096069750505 1103 60-61 0.25349397590444767 1103 62-63 0.35216940468868074 1103 64-65 0.3226181543443829 1103 66-67 0.5289790282747404 1103 68-69 0.49412967496972726 1103 70-71 0.4376562793323657 1103 72-73 0.39024533951427287 1103 74-75 0.3596171609406724 1103 76-77 0.4557057062118126 1103 78-79 0.577558712095307 1103 80-81 0.5693262229309788 1103 82-83 0.5504137179769799 1103 84-85 0.3646098513790399 1103 86-87 0.6097400535402038 1103 88-89 0.5618177769338573 1103 90-91 0.673350173569915 1103 92-93 0.8364841665116884 1103 94-95 0.6527603622753695 1103 96-97 0.8291252527259338 1103 98-99 0.9178720661419639 1103 100-101 1.2328062230402708 1104 1 0.14554177868360085 1104 2 0.1579770390905999 1104 3 0.2263871110405411 1104 4 -0.01047527753681976 1104 5 0.1696455873036058 1104 6 0.07794452213762071 1104 7 0.12103983670776586 1104 8 0.28839044345135534 1104 9 0.22612812693825646 1104 10-11 0.20511347663141777 1104 12-13 0.31042156094982687 1104 14-15 0.34536954640238804 1104 16-17 0.23580604530677363 1104 18-19 0.2669991776485503 1104 20-21 0.26615345258052514 1104 22-23 0.3347851679499172 1104 24-25 0.38468608097004875 1104 26-27 0.29892314771991124 1104 28-29 0.29236487529783517 1104 30-31 0.21343073369893517 1104 32-33 0.2551904510054115 1104 34-35 0.275414121981143 1104 36-37 0.22385126530652144 1104 38-39 0.21569886560655505 1104 40-41 0.1787243510003904 1104 42-43 0.3495596051942442 1104 44-45 0.4120089387432344 1104 46-47 0.4050859125293087 1104 48-49 0.5681901858710816 1104 50-51 0.5477869444567176 1104 52-53 0.4204295689718407 1104 54-55 0.5579556558471168 1104 56-57 0.5715689192792865 1104 58-59 0.38060275441684155 1104 60-61 0.49213150813056217 1104 62-63 0.6204661641593461 1104 64-65 0.5595969483357877 1104 66-67 0.5629572433647709 1104 68-69 0.5425712556701328 1104 70-71 0.4787896795449953 1104 72-73 0.5667770011050735 1104 74-75 0.611173662554517 1104 76-77 0.500215611657918 1104 78-79 0.6735682477876352 1104 80-81 0.760652230704796 1104 82-83 0.7214540265326157 1104 84-85 0.9261649267141259 1104 86-87 0.9377023086906888 1104 88-89 0.8390160740289225 1104 90-91 1.0510745158336725 1104 92-93 0.875268221913629 1104 94-95 1.0033290160240256 1104 96-97 0.9866302357124646 1104 98-99 0.9229440452775428 1104 100-101 1.148754416760088 1105 1 0.11462129428146639 1105 2 0.14138815526139936 1105 3 0.1408280481553419 1105 4 0.2107001178786092 1105 5 0.2820957637634791 1105 6 0.16564958727576595 1105 7 0.19182467127482994 1105 8 0.23815002655882012 1105 9 0.2499271526579676 1105 10-11 0.20721710801828408 1105 12-13 0.26648297282329025 1105 14-15 0.31617936992675055 1105 16-17 0.29991106356763453 1105 18-19 0.28193586046563013 1105 20-21 0.21666329562832232 1105 22-23 0.4180680284944529 1105 24-25 0.33793439579202555 1105 26-27 0.216488821248511 1105 28-29 0.24793445666842473 1105 30-31 0.4320270243496793 1105 32-33 0.21708833472189326 1105 34-35 0.3520540190192847 1105 36-37 0.4151492222293385 1105 38-39 0.37488528979712754 1105 40-41 0.3767095473423794 1105 42-43 0.42733714356385377 1105 44-45 0.41646287221794864 1105 46-47 0.4065295437742762 1105 48-49 0.3643704227327689 1105 50-51 0.45933556085415006 1105 52-53 0.37445560268302813 1105 54-55 0.4128104768371088 1105 56-57 0.5813548813712259 1105 58-59 0.7095760051964035 1105 60-61 0.6819550902962774 1105 62-63 0.6956069208333844 1105 64-65 0.6426779396222386 1105 66-67 0.728626738522145 1105 68-69 0.736872617239861 1105 70-71 0.775080934644933 1105 72-73 0.6509018265962183 1105 74-75 0.5417277473556403 1105 76-77 0.6236198722882769 1105 78-79 0.7424632760361902 1105 80-81 0.7035409673067363 1105 82-83 0.6921955311411203 1105 84-85 0.6850024383184881 1105 86-87 0.5535566065154391 1105 88-89 0.7150789258549146 1105 90-91 0.815554315900993 1105 92-93 0.6328903794197345 1105 94-95 0.6790490776897293 1105 96-97 0.7882187687503084 1105 98-99 0.7901037599251985 1105 100-101 1.1542877234140114 1106 1 0.10540761259523634 1106 2 0.21278080190963067 1106 3 0.08740826404642732 1106 4 0.09963665604839633 1106 5 0.05954971766575312 1106 6 0.030038618315685994 1106 7 0.1350283220702977 1106 8 0.15981460876864872 1106 9 0.12035345914068785 1106 10-11 0.03803236012281985 1106 12-13 -0.01537296188667625 1106 14-15 0.10121521684729373 1106 16-17 -0.02518651387281068 1106 18-19 0.18644008802457535 1106 20-21 0.047858865488223046 1106 22-23 0.13468460763943568 1106 24-25 0.159806881854216 1106 26-27 0.3416677052545438 1106 28-29 0.3533098851932266 1106 30-31 0.41188795219432706 1106 32-33 0.4771042454382908 1106 34-35 0.3943130347731909 1106 36-37 0.13042174105638793 1106 38-39 0.2765025127651626 1106 40-41 0.4372976797073065 1106 42-43 0.43855066078835847 1106 44-45 0.3592292332917637 1106 46-47 0.49524963628081053 1106 48-49 0.7081858807331898 1106 50-51 0.6424068072063491 1106 52-53 0.5322346426089837 1106 54-55 0.747974482793424 1106 56-57 0.7610544231731922 1106 58-59 0.6454619057263784 1106 60-61 0.7793291846412131 1106 62-63 0.9252892037152165 1106 64-65 0.8113816528876754 1106 66-67 0.9311477360781666 1106 68-69 0.9212820275106779 1106 70-71 0.9933773501441081 1106 72-73 1.0346426808028113 1106 74-75 1.2221899963761977 1106 76-77 1.0642930912888353 1106 78-79 1.079340345353625 1106 80-81 1.0989139830886892 1106 82-83 1.0936476549285352 1106 84-85 1.2292659111128224 1106 86-87 1.1954097906348125 1106 88-89 1.446888693351923 1106 90-91 1.463712344502035 1106 92-93 1.3582259801576484 1106 94-95 1.4296597534234614 1106 96-97 1.5496587395806642 1106 98-99 1.6149050034598744 1106 100-101 1.8319171509724406 1107 1 0.17992302409825456 1107 2 0.12412734647829282 1107 3 0.32068398278449095 1107 4 0.23579045652535768 1107 5 0.24307065952796592 1107 6 0.3013171006284878 1107 7 0.21166777025407413 1107 8 0.3008820996931476 1107 9 0.36250367114314486 1107 10-11 0.28552034772043555 1107 12-13 0.3094034405231909 1107 14-15 0.38009132299937676 1107 16-17 0.40268550950684556 1107 18-19 0.44720450852445737 1107 20-21 0.5217030027618179 1107 22-23 0.38058719082752646 1107 24-25 0.4332535345906692 1107 26-27 0.48639689258381935 1107 28-29 0.31916942594552467 1107 30-31 0.4010787961277842 1107 32-33 0.4737653271804092 1107 34-35 0.5399206841939872 1107 36-37 0.4620801988468699 1107 38-39 0.5459155420089843 1107 40-41 0.5617903422719408 1107 42-43 0.7041728021254627 1107 44-45 0.6673846675196557 1107 46-47 0.6821223353079446 1107 48-49 0.6702182235448504 1107 50-51 0.6084266336885236 1107 52-53 0.6301321110974882 1107 54-55 0.6821809019259604 1107 56-57 0.6861628718159523 1107 58-59 0.7628757165386659 1107 60-61 0.7684875300912708 1107 62-63 0.7983934588499793 1107 64-65 0.8432564462615133 1107 66-67 0.8510768893843377 1107 68-69 0.7855274367989935 1107 70-71 0.791657458183515 1107 72-73 0.9143381098309362 1107 74-75 0.6429717333519314 1107 76-77 0.6477092756940586 1107 78-79 0.8819690313235888 1107 80-81 0.8431782013757854 1107 82-83 0.9321773352718488 1107 84-85 0.9926320157563318 1107 86-87 0.9732625957755126 1107 88-89 1.0379759240959707 1107 90-91 1.0896235056848838 1107 92-93 1.132854012948215 1107 94-95 1.1390502905070399 1107 96-97 1.2776183483791677 1107 98-99 1.5171828662954105 1107 100-101 1.6940098366471084 1108 1 -0.17215536338601822 1108 2 -0.20631791815569755 1108 3 -0.18802059987101316 1108 4 -0.2027721715361821 1108 5 -0.09866630311617541 1108 6 -0.07817814214185148 1108 7 -0.01359706478004341 1108 8 -0.1547067027649618 1108 9 -0.0654067498620492 1108 10-11 -0.07129332200602789 1108 12-13 -0.024202839455568892 1108 14-15 0.05329511068904935 1108 16-17 0.03929640849676019 1108 18-19 0.04082392344970742 1108 20-21 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-1.578441227816704 2316 62-63 -1.466401137193877 2316 64-65 -1.6092175684927952 2316 66-67 -1.8252443093763802 2316 68-69 -1.4607413310751767 2316 70-71 -1.3942883695528572 2316 72-73 -1.839844377278002 2316 74-75 -2.258502343691138 2316 76-77 -1.9930643371623447 2316 78-79 -1.6600325502692712 2316 80-81 -1.6426158990661968 2316 82-83 -1.5280905605596793 2316 84-85 -1.7514921907390288 2316 86-87 -1.831289493876973 2316 88-89 -1.79336541018343 2316 90-91 -1.8920720620636189 2316 92-93 -1.9317966418962271 2316 94-95 -1.9045284831085247 2316 96-97 -1.8762145769176506 2316 98-99 -2.110092366068745 2316 100-101 -2.3207817126639227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10610.0 3 2272.0 4 2001.0 5 2240.0 6 2477.0 7 2945.0 8 3506.0 9 4547.0 10 5418.0 11 6617.0 12 8282.0 13 10230.0 14 11644.0 15 13854.0 16 16700.0 17 21321.0 18 18606.0 19 14089.0 20 14318.0 21 15472.0 22 16708.0 23 18538.0 24 20597.0 25 24112.0 26 27511.0 27 32525.0 28 38889.0 29 46649.0 30 56582.0 31 70689.0 32 89259.0 33 116555.0 34 158418.0 35 223839.0 36 335067.0 37 484083.0 38 409051.0 39 53245.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.05425951811975 18.872970009797836 14.798478150162126 23.274292321920292 2 14.900967679633439 19.757014511864565 41.002344275505656 24.339673532996343 3 18.929577277181192 24.09420998693794 29.978088377432695 26.998124358448173 4 12.568520962867039 16.42567517785584 37.616858706434115 33.38894515284301 5 13.84616601198488 36.785458323032394 34.67320432814166 14.695171336841062 6 31.95530266196796 35.93916690131091 17.85306542436227 14.252465012358856 7 28.509857569670988 32.115807674342285 21.030175198081018 18.34415955790571 8 26.122367319911287 35.038499228850796 20.137267885753456 18.70186556548446 9 25.82694555239921 16.8672769544946 20.634313925293117 36.67146356781307 10-11 24.399631957012847 26.64149959214947 28.0088699690982 20.949998481739485 12-13 25.356493439372667 23.826904010750074 28.511509513112415 22.305093036764845 14-15 22.290115416350595 25.251111244194245 26.57557957706179 25.88319376239337 16-17 21.966769015249135 28.73720677380686 27.051699482822162 22.244324728121846 18-19 21.88977560113004 27.185608894607764 29.321426001855677 21.603189502406515 20-21 22.38688741405107 26.334533559723134 29.608500223027917 21.670078803197878 22-23 22.1818222651892 26.27615978087119 29.45518699750315 22.08683095643646 24-25 22.051801241228652 26.438270114759693 29.46550898504907 22.044419658962582 26-27 22.032154977416734 26.72765261095164 29.160706052781144 22.07948635885048 28-29 21.81103591955009 26.6997698085953 29.471903193586 22.017291078268613 30-31 21.95230810058492 26.453996467698072 29.44823796700467 22.145457464712337 32-33 21.684951820785383 26.65391038853266 29.399948836232266 22.261188954449686 34-35 22.14763621269989 26.52094113125904 29.209406321704982 22.122016334336088 36-37 22.07861451207274 26.42180158846015 29.150680795369617 22.3489031040975 38-39 21.843286275801383 26.61771701939023 29.37014183795508 22.168854866853298 40-41 22.15105373829298 26.42809364409414 29.283937854215107 22.13691476339777 42-43 21.965489429274164 26.50745929765809 29.125683275990376 22.401367997077372 44-45 21.98617164882302 26.73816742632411 28.948511675196997 22.32714924965587 46-47 22.042387599375175 26.875797455130414 28.608260928150436 22.473554017343975 48-49 22.2354509458133 26.943138902290915 28.381261749879382 22.440148402016405 50-51 22.11163320607139 26.97847195401682 28.42579322419046 22.484101615721332 52-53 22.354941387087337 27.078377337824154 28.20489674022808 22.36178453486043 54-55 22.192280965645736 26.922005776702797 28.306800448342358 22.578912809309116 56-57 22.128510783041374 27.34754605398028 28.278667903616082 22.245275259362263 58-59 22.344365901893717 26.990401584275986 28.231620118026562 22.43361239580373 60-61 22.223626743630746 26.872615706518044 28.57517979202318 22.32857775782803 62-63 22.21645394978124 26.956386870571052 28.5387277237727 22.288431455875003 64-65 22.11831944024308 26.871138859176146 28.499140842566806 22.511400858013968 66-67 21.980208889760345 27.18299073197499 28.274182001613113 22.562618376651557 68-69 21.899153409232664 27.02185917320183 28.473018037872045 22.605969379693462 70-71 21.8991305591704 26.95522307996664 28.47234191810078 22.673304442762177 72-73 22.093086606235328 26.878255365374475 28.31460865746278 22.714049370927412 74-75 21.928767082273108 27.037345530258953 28.283083668675324 22.75080371879261 76-77 22.074767004235607 26.993281129750034 28.19317890460643 22.73877296140793 78-79 21.994965851589313 27.078603205760682 28.157503761055736 22.76892718159427 80-81 21.89287232016553 27.25073246106085 28.216217182709013 22.64017803606461 82-83 21.951915847118162 27.168656761108817 28.234374792063132 22.645052599709885 84-85 22.029974629942906 26.939911239103694 28.131678143102256 22.898435987851144 86-87 22.028001397159112 26.848574565051063 28.354949103489574 22.768474934300258 88-89 22.118946199203464 26.74372376715778 28.230228967254096 22.907101066384662 90-91 22.024701225205888 26.766632865923885 28.435746395875384 22.77291951299485 92-93 22.007802049758705 26.576103670240403 28.42838871516114 22.98770556483975 94-95 21.85659377732311 26.506478532939514 28.477973611791214 23.158954077946163 96-97 21.84885970296223 26.617195023976926 28.41544370204407 23.11850157101678 98-99 21.70731418241385 26.600049529315555 28.3575500912508 23.3350861970198 100-101 21.65503835679092 26.590694580154256 28.68004823184549 23.074218831209333 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3200.0 1 1816.0 2 406.5 3 355.0 4 305.5 5 305.0 6 470.0 7 956.0 8 1656.0 9 2103.5 10 2088.5 11 1985.5 12 2135.5 13 2362.0 14 2492.0 15 2609.0 16 2746.5 17 2916.5 18 3137.0 19 3356.5 20 3697.0 21 4227.5 22 5013.5 23 6044.5 24 7348.5 25 9216.0 26 12043.5 27 15606.5 28 18749.5 29 22578.5 30 28018.5 31 32656.5 32 36859.0 33 41638.5 34 47481.0 35 53008.0 36 58319.5 37 64035.0 38 67952.5 39 70136.0 40 72705.0 41 78760.0 42 84707.5 43 89739.5 44 94356.0 45 96010.5 46 95781.5 47 96521.0 48 100407.0 49 100056.0 50 95268.0 51 90081.0 52 83784.5 53 79432.0 54 75518.5 55 69133.0 56 62305.5 57 55845.5 58 49632.0 59 44207.0 60 37668.5 61 29662.0 62 22204.0 63 16601.5 64 12836.5 65 10071.0 66 7426.5 67 5504.0 68 3906.5 69 2538.5 70 1796.0 71 1289.0 72 951.5 73 730.0 74 518.0 75 345.5 76 235.0 77 147.0 78 90.5 79 57.0 80 43.0 81 32.5 82 23.5 83 20.0 84 19.5 85 12.5 86 8.0 87 8.0 88 7.0 89 7.0 90 5.0 91 5.0 92 5.5 93 2.5 94 2.5 95 3.5 96 2.5 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32878558921390694 2 0.29155753146019897 3 0.2944627310619376 4 0.2949192624279251 5 0.28134783000266034 6 0.21266061084726828 7 0.21913505567400007 8 0.2186785243080126 9 0.21344916502488306 10-11 0.22436441495712964 12-13 0.22397013786832226 14-15 0.2058956460603626 16-17 0.21876153001091939 18-19 0.20585414320890916 20-21 0.21023269403724382 22-23 0.2092781284538154 24-25 0.20085304960877334 26-27 0.2136774307078768 28-29 0.21454899058839838 30-31 0.22307782656207395 32-33 0.2252774776891046 34-35 0.21124951389785235 36-37 0.207763274375766 38-39 0.19742906436386712 40-41 0.19807235856139493 42-43 0.19877790703610293 44-45 0.21620910464653473 46-47 0.20539761184292168 48-49 0.21552430759755348 50-51 0.22314008083925405 52-53 0.232768742376445 54-55 0.22853545152819724 56-57 0.192241207932191 58-59 0.17997711532770858 60-61 0.18848519987565746 62-63 0.18904548837027849 64-65 0.18678358296606767 66-67 0.1995249583622643 68-69 0.18813242563830349 70-71 0.19305051353553246 72-73 0.20672570308943078 74-75 0.19564444175137052 76-77 0.2065389402578904 78-79 0.20317720929016428 80-81 0.1895435225877194 82-83 0.20323946356734438 84-85 0.18562150312537223 86-87 0.19062259672550802 88-89 0.19946270408508415 90-91 0.19479363329657565 92-93 0.20122657527185403 94-95 0.1992344384020904 96-97 0.19344479062433984 98-99 0.20072854105441315 100-101 0.18356711197842848 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2409473.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.52425475142425 #Duplication Level Percentage of deduplicated Percentage of total 1 77.19447726730743 42.08971343925698 2 12.625266727018875 13.767665186573147 3 4.227591415774477 6.915188140158659 4 1.8712193110082198 4.08107353636787 5 1.0736906162133675 2.927109034131566 6 0.657256899745116 2.1501865583300406 7 0.4230023085890515 1.6144719943765011 8 0.2986673805900141 1.3027693076184403 9 0.22615236112930145 1.1097710055765113 >10 1.2325706306119415 12.52848492926406 >50 0.10885896662960716 4.105297412419249 >100 0.05805097418695312 5.42883995597424 >500 0.0025865428727114325 0.9068600229979219 >1k 4.564487422427546E-4 0.5087840205865577 >5k 1.521495807475849E-4 0.5637854563682123 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7813 0.3242617784054853 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5779 0.23984497854925121 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3735 0.15501315017848302 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3566 0.1479991682828569 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 8.300570290681821E-5 0.0 0.0 0.0 0.0 7 8.300570290681821E-5 0.0 0.0 0.0 0.0 8 8.300570290681821E-5 0.0 0.0 0.0 0.0 9 8.300570290681821E-5 0.0 0.0 0.0 0.0 10-11 8.300570290681821E-5 0.0 0.0 0.0 0.0 12-13 8.300570290681821E-5 0.0 0.0 0.0 0.0 14-15 2.0751425726704554E-4 0.0 0.0 8.300570290681821E-5 0.0 16-17 2.0751425726704554E-4 0.0 0.0 1.0375712863352277E-4 0.0 18-19 2.0751425726704554E-4 0.0 0.0 1.2450855436022733E-4 0.0 20-21 2.0751425726704554E-4 0.0 0.0 1.4525998008693187E-4 0.0 22-23 2.0751425726704554E-4 0.0 0.0 1.8676283154034096E-4 2.0751425726704552E-5 24-25 2.9051996017386375E-4 0.0 0.0 3.73525663080682E-4 4.1502851453409105E-5 26-27 2.9051996017386375E-4 0.0 0.0 6.847970489812503E-4 4.1502851453409105E-5 28-29 4.1502851453409107E-4 0.0 0.0 0.0013695940979625005 4.1502851453409105E-5 30-31 4.980342174409093E-4 0.0 0.0 0.002739188195925001 4.1502851453409105E-5 32-33 5.187856431676139E-4 0.0 0.0 0.005084099303042615 4.1502851453409105E-5 34-35 5.602884946210229E-4 0.0 0.0 0.006681959083998866 4.1502851453409105E-5 36-37 5.810399203477275E-4 0.0 0.0 0.00929663872556364 4.1502851453409105E-5 38-39 6.225427718011366E-4 0.0 0.0 0.011496289852594321 4.1502851453409105E-5 40-41 6.225427718011366E-4 2.0751425726704552E-5 0.0 0.015252297909127846 4.1502851453409105E-5 42-43 6.432941975278411E-4 4.1502851453409105E-5 0.0 0.02149847705286592 4.1502851453409105E-5 44-45 7.055484747079548E-4 4.1502851453409105E-5 0.0 0.030961127184243195 6.225427718011366E-5 46-47 7.262999004346594E-4 4.1502851453409105E-5 0.0 0.053185904137543774 8.300570290681821E-5 48-49 7.47051326161364E-4 4.1502851453409105E-5 0.0 0.09647337820344948 8.300570290681821E-5 50-51 7.47051326161364E-4 4.1502851453409105E-5 0.0 0.12789103675368016 8.300570290681821E-5 52-53 7.47051326161364E-4 4.1502851453409105E-5 0.0 0.156631761385166 8.300570290681821E-5 54-55 7.88554177614773E-4 4.1502851453409105E-5 0.0 0.19431635050486146 8.300570290681821E-5 56-57 7.88554177614773E-4 4.1502851453409105E-5 0.0 0.22496620630320407 8.300570290681821E-5 58-59 7.88554177614773E-4 4.1502851453409105E-5 0.0 0.26918749452681145 8.300570290681821E-5 60-61 7.88554177614773E-4 4.1502851453409105E-5 0.0 0.33828974219673763 8.300570290681821E-5 62-63 7.88554177614773E-4 4.1502851453409105E-5 0.0 0.3900437979591388 8.300570290681821E-5 64-65 7.88554177614773E-4 4.1502851453409105E-5 0.0 0.45142651525873084 8.300570290681821E-5 66-67 7.88554177614773E-4 4.1502851453409105E-5 0.0 0.5657253681614195 8.300570290681821E-5 68-69 7.88554177614773E-4 4.1502851453409105E-5 0.0 0.6860006316733991 8.300570290681821E-5 70-71 8.093056033414777E-4 4.1502851453409105E-5 0.0 0.79600393945066 8.300570290681821E-5 72-73 8.508084547948866E-4 4.1502851453409105E-5 0.0 0.9261568816085509 8.300570290681821E-5 74-75 8.715598805215912E-4 4.1502851453409105E-5 0.0 1.0500636446227039 8.300570290681821E-5 76-77 8.715598805215912E-4 4.1502851453409105E-5 0.0 1.1579088041243875 8.300570290681821E-5 78-79 8.715598805215912E-4 4.1502851453409105E-5 0.0 1.3031480327855927 8.300570290681821E-5 80-81 9.545655834284094E-4 4.1502851453409105E-5 0.0 1.4814650340551647 8.300570290681821E-5 82-83 9.545655834284094E-4 4.1502851453409105E-5 0.0 1.6703237595939031 1.2450855436022733E-4 84-85 9.545655834284094E-4 4.1502851453409105E-5 0.0 1.8920112406322875 1.2450855436022733E-4 86-87 9.545655834284094E-4 4.1502851453409105E-5 0.0 2.2436856524227498 1.2450855436022733E-4 88-89 9.960684348818186E-4 4.1502851453409105E-5 0.0 2.737569584718318 1.2450855436022733E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3995 0.0 51.548195 1 GTATCAA 7510 0.0 43.507004 1 ATCAACG 9475 0.0 34.067722 3 TCAACGC 9520 0.0 33.85816 4 TATCAAC 9755 0.0 33.090557 2 CAACGCA 9755 0.0 33.037006 5 AACGCAG 9960 0.0 32.337517 6 ACGCAGA 11360 0.0 28.103691 7 CGCAGAG 11445 0.0 27.686842 8 GTACATG 8240 0.0 25.857895 1 GCAGAGT 12165 0.0 25.657104 9 TACATGG 8120 0.0 25.3509 2 ACATGGG 8325 0.0 24.040882 3 GAGTACT 7000 0.0 22.224243 12-13 AGAGTAC 11270 0.0 21.834671 10-11 CAGAGTA 12055 0.0 20.629576 10-11 AGTACTT 7700 0.0 20.574005 12-13 GTACTTT 7720 0.0 19.90353 14-15 CATGGGG 4865 0.0 19.641914 4 TGGTATC 2040 0.0 19.109322 2 >>END_MODULE