FastQCFastQC Report
Thu 26 May 2016
SRR937445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937445_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2409473
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83000.3444736670632956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69400.28802978908665916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43820.1818654950688387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41600.052.5581861
GTATCAA74850.041.0854761
ATCAACG94750.032.1361053
TCAACGC96200.031.70114
TATCAAC97250.031.3099822
CAACGCA99900.030.5745355
AACGCAG101400.030.1653396
ACGCAGA118850.025.577037
CGCAGAG119400.025.2603138
GTACATG73500.023.280431
GCAGAGT131550.022.5662029
TACATGG76850.022.2522012
TAGACCG1503.033874E-522.1677275
GAGTACT78050.021.63395912-13
ACATGGG77450.021.1598243
TGGTATC21800.021.1363332
GTGGTAT24600.020.8672161
AGAGTAC117800.020.28112410-11
GTACTTT83400.019.93252614-15
AGTACTT83250.019.59800312-13
Produced by FastQC (version 0.11.5)