##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937445_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2409473 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19010796136749 33.0 31.0 34.0 31.0 34.0 2 32.42045791756123 34.0 31.0 34.0 31.0 34.0 3 32.50757821316113 34.0 31.0 34.0 31.0 34.0 4 36.09950433144509 37.0 35.0 37.0 35.0 37.0 5 35.99550690130165 37.0 35.0 37.0 35.0 37.0 6 36.072573546165486 37.0 35.0 37.0 35.0 37.0 7 36.02558733797805 37.0 35.0 37.0 35.0 37.0 8 36.004234120905274 37.0 35.0 37.0 35.0 37.0 9 37.70592324545658 39.0 37.0 39.0 35.0 39.0 10-11 37.632355705998776 39.0 37.0 39.0 35.0 39.0 12-13 37.558461539100044 39.0 37.0 39.0 35.0 39.0 14-15 38.807211369457136 40.0 38.0 41.0 35.0 41.0 16-17 38.78219386562954 40.0 38.0 41.0 35.0 41.0 18-19 38.755031079410315 40.0 38.0 41.0 35.0 41.0 20-21 38.638727223753904 40.0 38.0 41.0 34.0 41.0 22-23 38.517640371981756 40.0 38.0 41.0 34.0 41.0 24-25 38.38160626825866 40.0 38.0 41.0 34.0 41.0 26-27 38.35785107365802 40.0 38.0 41.0 34.0 41.0 28-29 38.26481330149788 40.0 38.0 41.0 34.0 41.0 30-31 38.376383756946026 40.0 38.0 41.0 34.0 41.0 32-33 38.34068528678263 40.0 38.0 41.0 34.0 41.0 34-35 38.253303730732824 40.0 38.0 41.0 34.0 41.0 36-37 38.11763920990192 40.0 38.0 41.0 34.0 41.0 38-39 38.01300823873104 40.0 38.0 41.0 33.0 41.0 40-41 37.867134016442606 40.0 37.0 41.0 33.0 41.0 42-43 37.684851210202396 40.0 37.0 41.0 33.0 41.0 44-45 37.44204334308789 40.0 37.0 41.0 32.5 41.0 46-47 37.148470225646854 40.0 37.0 41.0 31.5 41.0 48-49 36.89160285257398 40.0 36.5 41.0 31.0 41.0 50-51 36.69594346979609 39.5 36.0 41.0 31.0 41.0 52-53 36.47300986564282 39.0 36.0 41.0 30.5 41.0 54-55 36.24223429770743 39.0 35.5 40.5 30.0 41.0 56-57 36.00620903409169 39.0 35.0 40.0 29.5 41.0 58-59 35.75677046391472 38.5 35.0 40.0 29.0 41.0 60-61 35.43926244452625 38.0 35.0 40.0 28.0 41.0 62-63 35.076087800112305 38.0 34.0 40.0 28.0 41.0 64-65 34.722997933573026 37.0 34.0 40.0 27.5 41.0 66-67 34.30185127619193 37.0 34.0 39.0 26.0 41.0 68-69 33.904303970204275 36.0 33.0 39.0 26.0 40.0 70-71 33.50559333929038 36.0 33.0 39.0 26.0 40.0 72-73 33.05438326140197 35.0 32.5 38.0 25.5 40.0 74-75 32.59220543247424 35.0 32.0 37.0 25.0 39.0 76-77 31.266059009584254 34.0 30.5 36.0 23.0 38.5 78-79 31.90548202864278 35.0 31.5 36.0 24.0 39.0 80-81 31.754568115102348 35.0 32.0 36.0 24.0 37.5 82-83 31.424012636788213 34.5 31.5 35.5 23.5 37.0 84-85 31.06303971864387 34.0 31.0 35.0 21.5 37.0 86-87 30.679988321097603 34.0 31.0 35.0 20.0 36.0 88-89 30.30401751752354 34.0 31.0 35.0 18.5 36.0 90-91 29.867320571759883 34.0 30.0 35.0 17.0 35.5 92-93 29.490547518067228 34.0 30.0 35.0 9.0 35.0 94-95 29.087163873593937 34.0 29.0 35.0 3.5 35.0 96-97 28.605473479055377 34.0 29.0 35.0 2.0 35.0 98-99 28.104168006862913 34.0 29.0 35.0 2.0 35.0 100-101 26.18575763247814 31.5 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1733962292578184 1101 2 0.2030433958223199 1101 3 0.19600321213254546 1101 4 0.0920459811524168 1101 5 0.04148730530918954 1101 6 0.10024372362982348 1101 7 0.12000614885831595 1101 8 0.12056702589956103 1101 9 0.2000159634400589 1101 10-11 0.11267578040259707 1101 12-13 0.11029978077367275 1101 14-15 0.12358301990123977 1101 16-17 0.05766721190427404 1101 18-19 0.062259140404549385 1101 20-21 0.11357446776315072 1101 22-23 0.05564087657630523 1101 24-25 0.1990190387258437 1101 26-27 0.1207517706018848 1101 28-29 0.15047273796838567 1101 30-31 0.2731218724181588 1101 32-33 0.18586822936465097 1101 34-35 0.07177049092418741 1101 36-37 0.019111030717496647 1101 38-39 -0.03753268196856396 1101 40-41 0.014553205545126957 1101 42-43 0.18292844437175404 1101 44-45 0.12661785682291793 1101 46-47 0.1398201552349505 1101 48-49 0.12816583067615284 1101 50-51 0.16836296713348275 1101 52-53 0.16382844092633064 1101 54-55 0.07693746774035759 1101 56-57 0.1381301898445031 1101 58-59 0.03169777251082451 1101 60-61 -0.0346367040455533 1101 62-63 0.03691118984468034 1101 64-65 0.03356974366991494 1101 66-67 0.1776533155075768 1101 68-69 0.058741625881289394 1101 70-71 -0.07706234282192526 1101 72-73 -0.1990219644997353 1101 74-75 -0.05048655986154671 1101 76-77 -0.05360478533016888 1101 78-79 -0.07355378102310439 1101 80-81 -0.05377006822858377 1101 82-83 0.06626097111011475 1101 84-85 0.027376970351845387 1101 86-87 0.05121863385816283 1101 88-89 0.08408407561369557 1101 90-91 0.15771992323485762 1101 92-93 0.17870551976051985 1101 94-95 0.09133256994015682 1101 96-97 0.13469414118680945 1101 98-99 0.23241946526117374 1101 100-101 0.5759686348749931 1102 1 0.08312296906339611 1102 2 0.10943294320640717 1102 3 0.0865851993791793 1102 4 0.04648301369739727 1102 5 -0.056329553393055676 1102 6 0.03952005271816006 1102 7 0.06390603308566511 1102 8 0.09924280736490942 1102 9 0.09847049051799672 1102 10-11 0.08118837756892106 1102 12-13 0.09843124704340056 1102 14-15 0.0706400057696257 1102 16-17 0.01030517430572786 1102 18-19 0.0723322793133434 1102 20-21 -0.0013023170651180749 1102 22-23 0.10394012129762586 1102 24-25 0.09354097121018157 1102 26-27 0.04574671703406352 1102 28-29 0.09630400393305649 1102 30-31 0.112584724100536 1102 32-33 0.02587870403249326 1102 34-35 0.1264519323854998 1102 36-37 0.10354787949636801 1102 38-39 -0.06824642951069393 1102 40-41 0.06383376125381801 1102 42-43 0.03543593284043567 1102 44-45 0.0826297648663683 1102 46-47 0.024083345046953752 1102 48-49 0.03079368594196552 1102 50-51 0.10224484228279351 1102 52-53 0.1417829404393487 1102 54-55 0.08468780311348922 1102 56-57 0.13237831083610274 1102 58-59 0.1267783539467615 1102 60-61 0.16282640490936728 1102 62-63 0.11658390241611727 1102 64-65 0.13224846539291235 1102 66-67 0.18044839792667489 1102 68-69 0.21870266746755362 1102 70-71 0.11968315950272057 1102 72-73 0.13977932921363134 1102 74-75 0.03308015642196693 1102 76-77 0.05665395734215295 1102 78-79 0.07341407828556612 1102 80-81 0.16869350014159679 1102 82-83 0.23840386763142263 1102 84-85 0.14271225160586454 1102 86-87 0.14328637004354405 1102 88-89 0.12998333503630022 1102 90-91 0.14340178735820075 1102 92-93 0.13113031348306947 1102 94-95 0.11225525954083437 1102 96-97 0.11639195620784903 1102 98-99 0.27681827445682927 1102 100-101 0.609509623904156 1103 1 0.14402457888301967 1103 2 0.13496654807558173 1103 3 0.0962293396542222 1103 4 0.14307957978496688 1103 5 -0.03028058458273364 1103 6 0.02956373858628325 1103 7 -0.010236181501532826 1103 8 0.003675121977046558 1103 9 0.012212605357333928 1103 10-11 -0.03508253022896213 1103 12-13 0.048183262781392955 1103 14-15 0.04393805791558947 1103 16-17 0.06927093677651897 1103 18-19 0.11435660670798597 1103 20-21 0.06039265767466162 1103 22-23 -0.02217904045091501 1103 24-25 0.0402543715962409 1103 26-27 0.08113227021907221 1103 28-29 -0.014019899533181501 1103 30-31 0.031828672268595426 1103 32-33 0.05701204290737394 1103 34-35 0.24079992311263965 1103 36-37 0.06775871691659319 1103 38-39 0.15991848695046684 1103 40-41 0.19788564861261904 1103 42-43 0.13611292525774843 1103 44-45 0.11732170099078587 1103 46-47 0.2701363776589645 1103 48-49 0.40008150469640924 1103 50-51 0.33063973297576155 1103 52-53 0.3968243635088129 1103 54-55 0.3935542795203517 1103 56-57 0.1770330262314488 1103 58-59 0.20866303433800226 1103 60-61 0.16074370654505543 1103 62-63 0.10979458282582755 1103 64-65 0.021000970634290184 1103 66-67 0.14700146286437388 1103 68-69 0.24550159240728675 1103 70-71 0.08207267489436987 1103 72-73 0.17782667250327933 1103 74-75 0.24602982993244638 1103 76-77 0.23572537865679877 1103 78-79 0.19438329498779083 1103 80-81 0.3276269369171594 1103 82-83 0.2878677228861939 1103 84-85 0.27011151088564844 1103 86-87 0.13817891019667528 1103 88-89 0.06870028201292655 1103 90-91 -0.01931745012483077 1103 92-93 0.24890298072236305 1103 94-95 0.2764393128135758 1103 96-97 0.06026346618725498 1103 98-99 0.1976323278219354 1103 100-101 0.4737768003932139 1104 1 0.1485092068340066 1104 2 0.11478734034347582 1104 3 0.07489125046026146 1104 4 0.02511985358255231 1104 5 -0.001388131994517039 1104 6 0.03655370309711259 1104 7 0.04354435614563812 1104 8 0.10841671981677337 1104 9 0.14305690245959113 1104 10-11 0.05457287362965957 1104 12-13 0.11465636304400562 1104 14-15 0.1940428714266389 1104 16-17 0.11962876494947494 1104 18-19 0.0468420832349139 1104 20-21 -0.10550759390174136 1104 22-23 0.07206274855811046 1104 24-25 0.060447122983866564 1104 26-27 0.03806482401314781 1104 28-29 0.033302411901388496 1104 30-31 0.023694928071087418 1104 32-33 0.051561440694122496 1104 34-35 0.05393625019312509 1104 36-37 0.02327206341004029 1104 38-39 -0.10784515588535726 1104 40-41 -0.00442833886728522 1104 42-43 -0.04806544828106496 1104 44-45 -1.7016981951911703E-4 1104 46-47 0.0294901295511778 1104 48-49 -0.06304433504019613 1104 50-51 -0.035681570475226465 1104 52-53 -0.004858825219024254 1104 54-55 0.05791037458482862 1104 56-57 0.05035466168178715 1104 58-59 0.10165623014928826 1104 60-61 0.15186655948611616 1104 62-63 0.2910270315067578 1104 64-65 0.03246345770488546 1104 66-67 0.1456237255178081 1104 68-69 0.1529531199039056 1104 70-71 0.08571777598637453 1104 72-73 0.08441200532362814 1104 74-75 0.07176924760319991 1104 76-77 0.17483849694564313 1104 78-79 0.25913137700241506 1104 80-81 0.26146336311801477 1104 82-83 0.2778257015974859 1104 84-85 0.3141005848711629 1104 86-87 0.3489748216906854 1104 88-89 0.35638316630815936 1104 90-91 0.26373080723818276 1104 92-93 0.16646185067810038 1104 94-95 0.2663904597694291 1104 96-97 0.3755488597742733 1104 98-99 0.4678498293411053 1104 100-101 0.8188400181020832 1105 1 0.07385515674415188 1105 2 0.07843944360698885 1105 3 0.05443052345765409 1105 4 0.09059880465596137 1105 5 -0.11824014875577404 1105 6 0.016073794245059503 1105 7 0.0241872423991083 1105 8 0.07765008435783471 1105 9 0.044650467448356324 1105 10-11 0.012372279307641065 1105 12-13 0.001046998517651332 1105 14-15 0.06027587939551893 1105 16-17 0.06007687397386263 1105 18-19 0.052797127811693656 1105 20-21 0.06452485232798466 1105 22-23 0.0831897978299736 1105 24-25 -0.004231708478883434 1105 26-27 0.14857244406853454 1105 28-29 0.03835702630547644 1105 30-31 0.11620438958401991 1105 32-33 0.09264850237901356 1105 34-35 0.016425683753105602 1105 36-37 -9.791152883238396E-4 1105 38-39 0.06517046208157495 1105 40-41 0.044505413068307575 1105 42-43 0.05593564902583381 1105 44-45 0.22394392434654975 1105 46-47 0.2674336802586623 1105 48-49 0.02594926426568378 1105 50-51 0.06572110883499249 1105 52-53 0.1376322952926472 1105 54-55 0.20837569209304974 1105 56-57 0.07853857935644015 1105 58-59 0.13143480328771773 1105 60-61 0.23719143301072876 1105 62-63 0.23376356144712673 1105 64-65 -0.03483855736153174 1105 66-67 0.06910141584445029 1105 68-69 0.09363390738977984 1105 70-71 0.11666959512202624 1105 72-73 0.2588667218382241 1105 74-75 0.2781672615197621 1105 76-77 0.26344208720977846 1105 78-79 0.2507258511582897 1105 80-81 0.21164264771911334 1105 82-83 0.22055520257247707 1105 84-85 0.21474885255308251 1105 86-87 0.2541026424407704 1105 88-89 0.3381619202289414 1105 90-91 0.3442467516907044 1105 92-93 0.23602279128242643 1105 94-95 0.313326803094057 1105 96-97 0.2866123461778862 1105 98-99 0.5614449047034356 1105 100-101 1.0233773926219136 1106 1 -0.06822878375894703 1106 2 0.030258849326429527 1106 3 0.027865650953742716 1106 4 0.046854374362872875 1106 5 -0.09855573679570995 1106 6 -0.11828347273242912 1106 7 -0.10795473142215428 1106 8 -0.09454804230829694 1106 9 -0.14728609494447653 1106 10-11 -0.28638440912151 1106 12-13 -0.3106398561453503 1106 14-15 -0.15544436737356904 1106 16-17 -0.03134663793431969 1106 18-19 -0.03733300094017267 1106 20-21 -0.03226884509047778 1106 22-23 0.01579395373969561 1106 24-25 0.021492050631849224 1106 26-27 -0.06627051410080753 1106 28-29 -0.06953576631847369 1106 30-31 0.03517583125046286 1106 32-33 -0.014205966199234865 1106 34-35 -0.2882836588206814 1106 36-37 -0.06926109257731383 1106 38-39 0.013272188037326771 1106 40-41 -0.14546015833421677 1106 42-43 -0.4450602050086445 1106 44-45 -0.26230487565651117 1106 46-47 -0.21874444263377768 1106 48-49 -0.16953063570588256 1106 50-51 -0.19692826130206953 1106 52-53 -0.050512563620863205 1106 54-55 -0.2378092260011826 1106 56-57 -0.1654034281221044 1106 58-59 -0.14136880390627482 1106 60-61 -0.0829128503887091 1106 62-63 -0.015063453495940848 1106 64-65 -0.03236581466254762 1106 66-67 -0.0986687539845974 1106 68-69 0.0036724634667635314 1106 70-71 -0.036964196409336125 1106 72-73 0.09680052152894092 1106 74-75 -0.25293935239165677 1106 76-77 -0.21124562673390201 1106 78-79 -0.03705086526761292 1106 80-81 0.0050843696207003575 1106 82-83 -0.1141956570269258 1106 84-85 -0.28743480721766446 1106 86-87 -0.09907597893645104 1106 88-89 -0.18665857425266807 1106 90-91 0.020882476603592437 1106 92-93 -0.14124788021865342 1106 94-95 -0.4494624073238924 1106 96-97 -0.2354128566263789 1106 98-99 -0.020624288426908777 1106 100-101 0.3407014440665641 1107 1 0.10618576208077002 1107 2 0.11855121049772777 1107 3 0.09815024137656536 1107 4 0.07414708218465904 1107 5 0.059865141834897884 1107 6 0.05539885419108259 1107 7 0.042063483777184274 1107 8 0.033103593207975734 1107 9 0.04723979631640418 1107 10-11 0.10022356769141538 1107 12-13 0.10749445808048108 1107 14-15 0.04607752456777092 1107 16-17 0.05558078693400148 1107 18-19 0.069396030717364 1107 20-21 0.20988497785457838 1107 22-23 0.1850532771934894 1107 24-25 0.13845489483492202 1107 26-27 0.06555176011897856 1107 28-29 0.1438657255625202 1107 30-31 0.07788585972074458 1107 32-33 0.1005046528607707 1107 34-35 0.07246154344024802 1107 36-37 -0.051779917624735106 1107 38-39 0.00437582850941709 1107 40-41 0.05628842055926242 1107 42-43 0.20723370646066996 1107 44-45 0.13653697468676285 1107 46-47 0.017006577256907462 1107 48-49 -0.028156014052427736 1107 50-51 0.08202628254760924 1107 52-53 0.1440265677628858 1107 54-55 0.008659873959338427 1107 56-57 0.03107437822223602 1107 58-59 0.08102473748154893 1107 60-61 0.2630609887333648 1107 62-63 0.08671758375935923 1107 64-65 0.25069978733775145 1107 66-67 0.2815765413584117 1107 68-69 0.4083642414307036 1107 70-71 0.18501428718735013 1107 72-73 0.21177132906409213 1107 74-75 0.24736657468977086 1107 76-77 0.17343651402545746 1107 78-79 0.15507354855603595 1107 80-81 0.2949333279038804 1107 82-83 0.32467710298501373 1107 84-85 0.21840950338117437 1107 86-87 0.3876660440901283 1107 88-89 0.4354727324591927 1107 90-91 0.5517512212473612 1107 92-93 0.7350442354085409 1107 94-95 0.6528141314993583 1107 96-97 0.5570752994818946 1107 98-99 0.7511510997092969 1107 100-101 1.098468380992223 1108 1 -0.1476819267885361 1108 2 -0.08249522924486996 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-0.1214886458014881 2316 90-91 -0.21365035167923452 2316 92-93 -0.009579635269158615 2316 94-95 0.02539028627879958 2316 96-97 0.054951726706956805 2316 98-99 -0.013535168056534985 2316 100-101 -0.3776408328694636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 16.0 5 52.0 6 90.0 7 177.0 8 443.0 9 925.0 10 1534.0 11 2563.0 12 3856.0 13 5466.0 14 7214.0 15 9847.0 16 13488.0 17 18981.0 18 17182.0 19 10697.0 20 9540.0 21 9803.0 22 10153.0 23 11357.0 24 13143.0 25 15910.0 26 19604.0 27 24241.0 28 31290.0 29 39235.0 30 50279.0 31 63593.0 32 82574.0 33 109255.0 34 150707.0 35 220955.0 36 349187.0 37 565811.0 38 492478.0 39 47808.0 40 5.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.32995210749051 19.8941406527384 15.016728949491737 22.75917829027935 2 15.486830522691061 20.798655971658533 39.6018133425857 24.112700163064705 3 19.40540524836759 24.49344732229828 29.931067914021032 26.1700795153131 4 13.409654310299391 17.300588136907948 36.77970244945679 32.51005510333587 5 14.056682017319922 37.30868760631926 34.178657029544816 14.455973346816005 6 32.32885365389029 36.58210737368711 17.100917918565596 13.988121053857006 7 28.77122922730406 32.53284016878379 20.570390288664782 18.12554031524736 8 26.471390216864847 35.639785131437456 19.501484349482233 18.387340302215463 9 26.0093804744855 17.129264366108274 20.304896547917327 36.5564586114889 10-11 24.735346691994472 27.12030805076463 27.363639268835964 20.780705988404932 12-13 25.674591242189198 24.205078144860337 28.10710202753503 22.013228585415437 14-15 22.525776383466425 25.80697106794722 26.124135858754176 25.543116689832175 16-17 22.186283058577537 29.29964353200887 26.559729036183434 21.954344373230164 18-19 22.106224058123914 27.642538430602876 28.90391384340061 21.3473236678726 20-21 22.6021416301407 26.808808399181068 29.315310858432532 21.273739112245707 22-23 22.44951235779437 26.779814156834895 29.053879908742697 21.71679357662804 24-25 22.395008030811756 26.936297193988718 29.10623498113693 21.562459794062594 26-27 22.3747907195264 27.230408981981753 28.889006020471097 21.50579427802076 28-29 22.046391786807625 27.203107671976994 29.12892547165426 21.621575069561118 30-31 22.229259261257546 26.910428130964736 29.134607443204384 21.72570516457333 32-33 22.044696080844233 27.130372492242078 29.071647617549562 21.75328380936412 34-35 22.47852538708672 26.97533859063787 28.864299371688336 21.681836650587076 36-37 22.38149172038865 26.88272497761959 28.70461715072134 22.031166151270423 38-39 22.17582434001128 27.143736410410078 28.966977426184066 21.713461823394574 40-41 22.46839869133209 26.989511814409212 28.84946210229374 21.692627391964965 42-43 22.334869986368535 27.081662607113266 28.664058258327064 21.919409148191132 44-45 22.357980355040294 27.2995588661919 28.458878767265706 21.8835820115021 46-47 22.44262542057952 27.459988138485052 28.09902829373892 21.9983581471965 48-49 22.592077188663247 27.597632345330286 27.921873372310046 21.88841709369642 50-51 22.50452692352228 27.655798591642238 27.812471855878858 22.027202628956623 52-53 22.749186232840128 27.745797525018958 27.543865401272395 21.96115084086852 54-55 22.514964890662814 27.67607273457723 27.718592405891247 22.09036996886871 56-57 22.475225910396173 28.00828230903604 27.673333546381308 21.84315823418648 58-59 22.696954894286012 27.623301858954218 27.614108977357287 22.06563426940248 60-61 22.572550097054418 27.58061617623439 27.977964476049326 21.868869250661866 62-63 22.518658644442166 27.725274364975245 27.957337558876983 21.798729431705606 64-65 22.483671740666942 27.604169044434197 27.869787293736014 22.042371921162843 66-67 22.368210807923557 27.931896310936043 27.66210702506316 22.037785856077242 68-69 22.349970304709785 27.72790979604254 27.825752768343946 22.09636713090373 70-71 22.26845870445529 27.720563791335284 27.868251688232238 22.142725815977187 72-73 22.461606207004316 27.737368962446745 27.61001738348015 22.191007447068785 74-75 22.33185431005037 27.973959450884074 27.501636249918548 22.192549989147004 76-77 22.493238147926952 27.81228509304732 27.52892437474917 22.165552384276562 78-79 22.431440402112827 27.798900423453592 27.497133190535855 22.272525983897722 80-81 22.34206401150791 28.16634591879635 27.39337606190233 22.098214007793406 82-83 22.38852459662751 28.044859723096376 27.440742587622612 22.125873092653496 84-85 22.40933327937894 27.846690676370155 27.34495635401135 22.399019690239548 86-87 22.372589358751892 27.762585428431862 27.57671490819777 22.288110304618478 88-89 22.43401357890294 27.674952157587988 27.441291103905296 22.44974315960378 90-91 22.356873609381935 27.73100732994898 27.538476576808606 22.373642483860475 92-93 22.26361938881513 27.60031227030556 27.507199772894324 22.628868567984988 94-95 22.189769298099627 27.432015216605453 27.615520074306705 22.76269541098821 96-97 22.162501924694737 27.63324179187731 27.57337392865577 22.630882354772183 98-99 22.047040664777313 27.712377231742185 27.315837496941754 22.924744606538745 100-101 21.849423856757095 28.04422193318727 27.381482350846237 22.7248718592094 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 232.0 1 188.5 2 172.0 3 224.5 4 218.5 5 221.5 6 422.5 7 856.5 8 1422.5 9 1867.5 10 1984.0 11 2023.5 12 2207.0 13 2449.5 14 2782.5 15 2961.5 16 3073.5 17 3285.5 18 3454.5 19 3629.5 20 3923.5 21 4456.5 22 5192.0 23 6230.0 24 7527.0 25 9217.0 26 11749.5 27 15263.0 28 18781.5 29 23043.5 30 28496.0 31 32843.0 32 37179.5 33 42073.0 34 47923.0 35 53407.5 36 58853.0 37 64441.0 38 67840.5 39 70202.5 40 72928.0 41 78881.5 42 85016.0 43 89730.5 44 94650.0 45 96531.5 46 95861.0 47 96809.0 48 100634.5 49 100489.5 50 96313.5 51 90947.5 52 84523.0 53 79359.5 54 74895.5 55 68911.0 56 61375.5 57 54531.0 58 48731.5 59 43359.0 60 37048.5 61 29320.0 62 22124.0 63 16982.0 64 12724.5 65 9798.0 66 7427.5 67 5619.0 68 3919.5 69 2276.5 70 1602.5 71 1139.0 72 862.5 73 638.5 74 472.5 75 343.5 76 197.5 77 107.0 78 58.5 79 43.5 80 32.5 81 23.0 82 13.0 83 9.0 84 4.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05694191219407729 2 0.0 3 0.0 4 0.0 5 0.018883797411301143 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002884448176011933 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.130627319750003E-4 24-25 1.2450855436022733E-4 26-27 0.001120576989242046 28-29 0.0033617309677261373 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.014152472345612505 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 6.225427718011366E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.697685344471592E-4 84-85 0.0012658369693289778 86-87 0.0 88-89 0.0 90-91 0.010271955734718754 92-93 9.130627319750003E-4 94-95 0.0 96-97 0.0 98-99 1.86762831540341E-4 100-101 0.012720623970469892 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2409473.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.21046280060847 #Duplication Level Percentage of deduplicated Percentage of total 1 75.48313670890482 39.41009501213519 2 13.517783622512558 14.115394779397326 3 4.531857760657971 7.098311730914454 4 2.027775793055931 4.23484450445284 5 1.1516615960669134 3.0064392460170475 6 0.6861835633542387 2.1495576845337268 7 0.46539916917619634 1.7009094206795523 8 0.32921088619857775 1.3750602181940954 9 0.2515846740457296 1.1821817038920994 >10 1.3684032438452038 13.219042503994386 >50 0.11549391209584188 4.173104660432343 >100 0.06776282773395478 6.131640438038188 >500 0.0031085840793039234 1.0812550098126419 >1k 4.7824370450806934E-4 0.4879422288526131 >5k 1.5941456816935643E-4 0.634220858653631 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8300 0.3444736670632956 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6940 0.28802978908665916 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4382 0.1818654950688387 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 8.300570290681821E-5 0.0 16-17 2.0751425726704552E-5 0.0 0.0 1.0375712863352277E-4 0.0 18-19 4.1502851453409105E-5 0.0 0.0 1.2450855436022733E-4 0.0 20-21 4.1502851453409105E-5 0.0 0.0 1.4525998008693187E-4 0.0 22-23 4.1502851453409105E-5 0.0 0.0 1.8676283154034096E-4 2.0751425726704552E-5 24-25 8.300570290681821E-5 0.0 0.0 3.73525663080682E-4 6.225427718011366E-5 26-27 8.300570290681821E-5 0.0 0.0 7.262999004346594E-4 1.0375712863352277E-4 28-29 1.2450855436022733E-4 0.0 0.0 0.0014110969494159097 1.2450855436022733E-4 30-31 1.2450855436022733E-4 0.0 0.0 0.0028221938988318195 1.2450855436022733E-4 32-33 1.2450855436022733E-4 0.0 0.0 0.005187856431676139 1.2450855436022733E-4 34-35 1.6601140581363642E-4 0.0 0.0 0.006764964786905685 1.2450855436022733E-4 36-37 2.9051996017386375E-4 0.0 0.0 0.009441898705650572 1.2450855436022733E-4 38-39 3.3202281162727284E-4 4.1502851453409105E-5 0.0 0.011786809812768187 1.2450855436022733E-4 40-41 3.3202281162727284E-4 4.1502851453409105E-5 0.0 0.015688077849388644 1.2450855436022733E-4 42-43 4.357799402607956E-4 4.1502851453409105E-5 0.0 0.021851251290219895 1.2450855436022733E-4 44-45 4.980342174409093E-4 4.1502851453409105E-5 0.0 0.031106387164330125 1.2450855436022733E-4 46-47 5.602884946210229E-4 4.1502851453409105E-5 0.0 0.05339341839481082 1.2450855436022733E-4 48-49 6.225427718011366E-4 4.1502851453409105E-5 0.0 0.09690915814371026 1.2450855436022733E-4 50-51 6.225427718011366E-4 4.1502851453409105E-5 0.0 0.12830606526821425 1.6601140581363642E-4 52-53 6.432941975278411E-4 4.1502851453409105E-5 0.0 0.1572335527312404 1.6601140581363642E-4 54-55 6.640456232545457E-4 4.1502851453409105E-5 0.0 0.19512565610820293 1.6601140581363642E-4 56-57 7.055484747079548E-4 4.1502851453409105E-5 0.0 0.22585851760945236 1.6601140581363642E-4 58-59 7.055484747079548E-4 4.1502851453409105E-5 0.0 0.27010055725878646 1.6601140581363642E-4 60-61 7.055484747079548E-4 4.1502851453409105E-5 0.0 0.33974234199760694 1.6601140581363642E-4 62-63 7.055484747079548E-4 4.1502851453409105E-5 0.0 0.3912681320770144 1.6601140581363642E-4 64-65 7.055484747079548E-4 4.1502851453409105E-5 0.0 0.4531281321683206 1.6601140581363642E-4 66-67 7.88554177614773E-4 4.1502851453409105E-5 0.0 0.5685060592087979 1.6601140581363642E-4 68-69 7.88554177614773E-4 4.1502851453409105E-5 0.0 0.6903584310760071 1.6601140581363642E-4 70-71 7.88554177614773E-4 4.1502851453409105E-5 0.0 0.8002579817246345 1.6601140581363642E-4 72-73 7.88554177614773E-4 4.1502851453409105E-5 0.0 0.9312409809115936 1.6601140581363642E-4 74-75 7.88554177614773E-4 4.1502851453409105E-5 0.0 1.055749535271821 1.6601140581363642E-4 76-77 7.88554177614773E-4 4.1502851453409105E-5 0.0 1.1643624975253926 1.6601140581363642E-4 78-79 7.88554177614773E-4 4.1502851453409105E-5 0.0 1.3105147889185726 1.6601140581363642E-4 80-81 9.130627319750003E-4 4.1502851453409105E-5 0.0 1.4904088985433743 1.6601140581363642E-4 82-83 9.130627319750003E-4 4.1502851453409105E-5 0.0 1.6806164667543484 1.6601140581363642E-4 84-85 9.130627319750003E-4 4.1502851453409105E-5 0.0 1.9038395532965091 1.6601140581363642E-4 86-87 9.130627319750003E-4 4.1502851453409105E-5 0.0 2.2592907245692313 1.6601140581363642E-4 88-89 9.130627319750003E-4 4.1502851453409105E-5 0.0 2.7609149386608607 1.6601140581363642E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4160 0.0 52.558186 1 GTATCAA 7485 0.0 41.085476 1 ATCAACG 9475 0.0 32.136105 3 TCAACGC 9620 0.0 31.7011 4 TATCAAC 9725 0.0 31.309982 2 CAACGCA 9990 0.0 30.574535 5 AACGCAG 10140 0.0 30.165339 6 ACGCAGA 11885 0.0 25.57703 7 CGCAGAG 11940 0.0 25.260313 8 GTACATG 7350 0.0 23.28043 1 GCAGAGT 13155 0.0 22.566202 9 TACATGG 7685 0.0 22.252201 2 TAGACCG 150 3.033874E-5 22.167727 5 GAGTACT 7805 0.0 21.633959 12-13 ACATGGG 7745 0.0 21.159824 3 TGGTATC 2180 0.0 21.136333 2 GTGGTAT 2460 0.0 20.867216 1 AGAGTAC 11780 0.0 20.281124 10-11 GTACTTT 8340 0.0 19.932526 14-15 AGTACTT 8325 0.0 19.598003 12-13 >>END_MODULE