FastQCFastQC Report
Thu 26 May 2016
SRR937444_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937444_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658564
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80810.30396108575907893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61280.23050037539062443No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38460.14466456327551264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37780.14210679148593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42700.049.746741
GTATCAA80450.045.0695761
ATCAACG99200.036.3031433
TATCAAC101450.035.5903172
TCAACGC101750.035.442034
CAACGCA104200.034.5162055
AACGCAG106200.033.9550176
ACGCAGA122150.029.0528857
CGCAGAG122600.028.6346288
GTACATG90450.026.0590211
GCAGAGT133700.025.7584449
TACATGG92600.024.4724662
ACATGGG91950.024.0263583
GAGTACT75550.022.54092612-13
TGGTATC21950.021.6439132
AGAGTAC121800.020.8262510-11
CAGAGTA129850.020.65090610-11
GTACTTT82950.020.44318214-15
AGTACTT81050.020.01496312-13
GTGGTAT27200.019.7421531
Produced by FastQC (version 0.11.5)