##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937444_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2658564 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.698645208465923 33.0 31.0 34.0 30.0 34.0 2 31.801536468559718 33.0 31.0 34.0 30.0 34.0 3 31.850566696908558 33.0 31.0 34.0 30.0 34.0 4 35.44386405593396 37.0 35.0 37.0 33.0 37.0 5 35.44899953508736 37.0 35.0 37.0 33.0 37.0 6 35.466116670503325 37.0 35.0 37.0 33.0 37.0 7 35.35255611676078 37.0 35.0 37.0 33.0 37.0 8 35.35686257694003 37.0 35.0 37.0 33.0 37.0 9 36.98398270645356 39.0 37.0 39.0 33.0 39.0 10-11 36.87083290076899 39.0 37.0 39.0 33.0 39.0 12-13 36.72404350619357 39.0 37.0 39.0 32.5 39.0 14-15 37.91348675450356 40.0 38.0 41.0 33.0 41.0 16-17 37.83734207639914 40.0 38.0 41.0 33.0 41.0 18-19 37.773801571073705 40.0 38.0 41.0 33.0 41.0 20-21 37.649337386649336 40.0 37.5 41.0 32.0 41.0 22-23 37.49347636543638 40.0 37.0 41.0 32.0 41.0 24-25 37.43138118924352 40.0 37.0 41.0 32.0 41.0 26-27 37.468695694367334 40.0 37.0 41.0 32.0 41.0 28-29 37.38313521886251 40.0 37.0 41.0 32.0 41.0 30-31 37.19000031596005 39.5 37.0 41.0 31.0 41.0 32-33 36.98842721108087 39.0 36.0 41.0 31.0 41.0 34-35 37.041384559483994 40.0 37.0 41.0 31.0 41.0 36-37 36.931475977256895 40.0 36.0 41.0 30.5 41.0 38-39 36.832152056523746 40.0 36.0 41.0 30.0 41.0 40-41 36.63446864547929 39.5 36.0 41.0 30.0 41.0 42-43 36.38828442723215 39.0 36.0 41.0 30.0 41.0 44-45 36.1974498262972 39.0 35.5 41.0 29.5 41.0 46-47 35.90990380521214 39.0 35.0 41.0 28.5 41.0 48-49 35.62451985357509 39.0 35.0 40.0 27.0 41.0 50-51 34.681931674392644 38.0 34.0 39.5 25.5 40.5 52-53 34.81431742850651 38.0 34.5 39.5 26.0 40.5 54-55 35.19020268084574 39.0 35.0 40.0 26.0 41.0 56-57 34.979376648446305 38.5 34.5 40.0 26.0 41.0 58-59 34.70382131105364 38.0 34.0 40.0 25.5 41.0 60-61 34.39435951137531 38.0 34.0 40.0 24.5 41.0 62-63 34.07639857456883 37.5 34.0 40.0 23.0 41.0 64-65 33.64484435958661 37.0 33.0 40.0 22.0 41.0 66-67 33.285126857957906 36.5 33.0 39.0 22.0 41.0 68-69 32.81121782285474 36.0 32.0 39.0 20.5 40.5 70-71 32.337798149677795 35.5 31.5 39.0 18.5 40.0 72-73 31.86632012620347 35.0 31.0 38.0 17.5 40.0 74-75 31.359130906760193 35.0 31.0 37.0 13.0 39.0 76-77 30.86561579860406 35.0 30.5 37.0 9.0 39.0 78-79 30.34742834853703 34.0 30.0 36.0 7.0 38.5 80-81 29.843464178406087 34.0 29.5 36.0 3.5 37.5 82-83 29.356124772621612 34.0 29.0 35.0 2.0 37.0 84-85 28.818739364559214 34.0 29.0 35.0 2.0 36.5 86-87 28.339474618628703 34.0 27.5 35.0 2.0 36.0 88-89 27.872433388852027 33.5 27.0 35.0 2.0 36.0 90-91 27.35389180023501 33.0 26.0 35.0 2.0 35.0 92-93 26.81416358605623 33.0 25.0 35.0 2.0 35.0 94-95 26.251132002088347 32.5 24.5 35.0 2.0 35.0 96-97 25.501736651816543 32.0 21.5 34.0 2.0 35.0 98-99 24.554704156078245 32.0 10.0 34.0 2.0 35.0 100-101 21.997087713517523 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04876641195110665 1101 2 -0.0048256786119722506 1101 3 -0.03152892402265195 1101 4 -0.10890413883721806 1101 5 -0.1508657649322629 1101 6 -0.11478297895426692 1101 7 -0.027927029801702474 1101 8 -0.2275861499522236 1101 9 -0.18315787644721127 1101 10-11 -0.17292237736661775 1101 12-13 -0.09907477248757601 1101 14-15 -0.18409125985169084 1101 16-17 -0.2526789161978229 1101 18-19 -0.003757968265489353 1101 20-21 0.10390921395318742 1101 22-23 -0.014233479297779184 1101 24-25 -7.866070827304839E-4 1101 26-27 -0.1678414961103485 1101 28-29 -0.2063027306431664 1101 30-31 -0.14586472897094183 1101 32-33 -0.3424568111424975 1101 34-35 -0.30158463750384357 1101 36-37 -0.1447703944480523 1101 38-39 -0.15823806861113354 1101 40-41 -0.21976212188317135 1101 42-43 -0.21171290336774717 1101 44-45 -0.10084360457254604 1101 46-47 -0.1884688562224568 1101 48-49 -0.26979071706951885 1101 50-51 -0.21297516776304093 1101 52-53 -0.26688328047330856 1101 54-55 -0.2432626100330495 1101 56-57 -0.3323140445084434 1101 58-59 -0.46924036135038705 1101 60-61 -0.30730069945246896 1101 62-63 -0.4818414792822594 1101 64-65 -0.53887278889421 1101 66-67 -0.565111903830406 1101 68-69 -0.5744686187530377 1101 70-71 -0.5593324842934138 1101 72-73 -0.5597721630118642 1101 74-75 -0.5425783578826433 1101 76-77 -0.3600583952662042 1101 78-79 -0.4777250538920761 1101 80-81 -0.4586070730752603 1101 82-83 -0.5385027401076812 1101 84-85 -0.5457075975236982 1101 86-87 -0.4980463210921471 1101 88-89 -0.4072212090085898 1101 90-91 -0.3524541002234507 1101 92-93 -0.3207823105305785 1101 94-95 -0.2960998835248638 1101 96-97 -0.27977571441712357 1101 98-99 -0.22473425034240435 1101 100-101 0.14495698312608596 1102 1 -0.05219800840473354 1102 2 -0.055802166012611565 1102 3 0.007923616543813239 1102 4 -0.1189896022039747 1102 5 0.020463045212729014 1102 6 -0.018140869631110945 1102 7 0.003475243941117867 1102 8 -0.10935527519189492 1102 9 -0.14490044934456847 1102 10-11 -0.14531531590457547 1102 12-13 -0.07204645918660901 1102 14-15 -0.016025662305871435 1102 16-17 -0.0029167279957889036 1102 18-19 -0.06305935740002155 1102 20-21 -0.09463242206889788 1102 22-23 -0.1752803038055788 1102 24-25 -0.05787012329095376 1102 26-27 -0.12406189246202359 1102 28-29 -0.27341597928135286 1102 30-31 -0.14482043146163193 1102 32-33 -0.26899921816711725 1102 34-35 -0.09709577108599632 1102 36-37 -0.17108492496726768 1102 38-39 0.004734326759120222 1102 40-41 -0.10486885034914906 1102 42-43 -0.14564792238574142 1102 44-45 -0.015795664056462044 1102 46-47 -0.07148961989211955 1102 48-49 -0.10242436164251245 1102 50-51 -0.2173961744453905 1102 52-53 -0.21267166926264736 1102 54-55 -0.2051657753716043 1102 56-57 -0.07748852948609652 1102 58-59 -0.08347587880047769 1102 60-61 0.03956964368930471 1102 62-63 -0.024791024472158085 1102 64-65 -0.20497102978167447 1102 66-67 -0.1583965095660318 1102 68-69 -0.12538937521688354 1102 70-71 -0.22927913742982042 1102 72-73 -0.08282313354404636 1102 74-75 -0.07919507216129418 1102 76-77 -0.08931432820407181 1102 78-79 -0.2384247486076312 1102 80-81 -0.13879769769251382 1102 82-83 -0.1745416094336676 1102 84-85 0.017513467996231213 1102 86-87 -0.07860541504110685 1102 88-89 -0.2380110181179731 1102 90-91 -0.22049728629013998 1102 92-93 -0.11484081699705584 1102 94-95 -0.013959509850668184 1102 96-97 0.1369751193633455 1102 98-99 0.17357227358160188 1102 100-101 0.41394778370025165 1103 1 -0.06841548374170259 1103 2 -0.1454076013168013 1103 3 -0.11486551946353174 1103 4 -0.17200541516009338 1103 5 -0.18266012912886254 1103 6 -0.10089199299624596 1103 7 -0.09269757315697547 1103 8 -0.06138736271060452 1103 9 -0.0768868009023933 1103 10-11 -0.1526437188198031 1103 12-13 -0.17020223141764035 1103 14-15 -0.2400182296520299 1103 16-17 -0.2878318531198545 1103 18-19 -0.2981417375095887 1103 20-21 -0.2681784230320261 1103 22-23 -0.23894422356160305 1103 24-25 -0.3904131982921797 1103 26-27 -0.38222347585546146 1103 28-29 -0.34827524319295833 1103 30-31 -0.30357597839540773 1103 32-33 -0.2891124984871922 1103 34-35 -0.18715176888103713 1103 36-37 -0.21118504000609306 1103 38-39 -0.37300264696975205 1103 40-41 -0.06155643015792123 1103 42-43 -0.28035463634839886 1103 44-45 -0.3011663668133693 1103 46-47 0.05966055810807802 1103 48-49 0.01712702003443667 1103 50-51 -0.062317526881933816 1103 52-53 0.04435579845664961 1103 54-55 0.06539334573649569 1103 56-57 -0.053293403755951374 1103 58-59 -0.1906135035801384 1103 60-61 -0.10049985764231906 1103 62-63 -0.04793643830087291 1103 64-65 -0.067041553562035 1103 66-67 0.06414817561589814 1103 68-69 0.11689266804586396 1103 70-71 0.10579679768876105 1103 72-73 0.12816810500289222 1103 74-75 0.2361561494365958 1103 76-77 0.20687259531469238 1103 78-79 0.21376718322399668 1103 80-81 0.09951203299542755 1103 82-83 0.03779795537364805 1103 84-85 0.13273667542709333 1103 86-87 0.30280061522964985 1103 88-89 0.33133158960007236 1103 90-91 0.33548476974521435 1103 92-93 0.25780605437063286 1103 94-95 -5.1507402002215485E-5 1103 96-97 0.013159213061566533 1103 98-99 0.2924882794499588 1103 100-101 0.8699897440061477 1104 1 -0.02468149022678645 1104 2 0.010756651915198034 1104 3 0.015546321430278454 1104 4 0.019554530360082367 1104 5 0.09567078014239172 1104 6 0.0814136695925356 1104 7 0.04107657848033597 1104 8 0.027928107123905477 1104 9 0.0806418096090269 1104 10-11 0.1448661267183553 1104 12-13 0.03066631491770977 1104 14-15 0.0477945241196025 1104 16-17 0.18659741268116647 1104 18-19 0.20266695794015988 1104 20-21 0.20170737108701076 1104 22-23 0.05936976282114159 1104 24-25 0.12017541526238773 1104 26-27 0.04587499673240103 1104 28-29 0.04553734256435149 1104 30-31 -0.016165379390606915 1104 32-33 -0.011486372192386796 1104 34-35 0.09642098486360595 1104 36-37 -0.00302811327821928 1104 38-39 0.07940847665808803 1104 40-41 0.06039565337150776 1104 42-43 -0.07975531778480871 1104 44-45 -0.07270619110007459 1104 46-47 -0.14136301349183356 1104 48-49 0.11864140938165946 1104 50-51 -0.09837329954033436 1104 52-53 -0.037385025616501366 1104 54-55 -0.2217883421866773 1104 56-57 -0.21717366419840545 1104 58-59 -0.16625733075750304 1104 60-61 -0.1585354800527341 1104 62-63 0.06433373547924504 1104 64-65 0.09390621591286674 1104 66-67 -0.032033108465626015 1104 68-69 0.006222678879076682 1104 70-71 -0.025523433901106785 1104 72-73 0.13158540838648847 1104 74-75 0.09095145704619512 1104 76-77 0.08887019790548933 1104 78-79 0.029146937767887238 1104 80-81 0.11466807208136132 1104 82-83 0.084684410416493 1104 84-85 0.23468915031519444 1104 86-87 0.34268731689592613 1104 88-89 0.3045758146137665 1104 90-91 0.6358572689717761 1104 92-93 0.5778470255743962 1104 94-95 0.6264676642806357 1104 96-97 0.7192073575997675 1104 98-99 0.7658504181306007 1104 100-101 0.9927537280778509 1105 1 0.15724925451115013 1105 2 0.13408337001707338 1105 3 0.10942422849187494 1105 4 0.0468650161977493 1105 5 0.0898087143369608 1105 6 0.045311612421862435 1105 7 0.032090860795605636 1105 8 -0.017278802992777287 1105 9 0.0702875637946292 1105 10-11 -0.0037591847388966926 1105 12-13 0.14456318858443495 1105 14-15 0.10649545422318596 1105 16-17 0.21316813982375038 1105 18-19 0.18058925934829517 1105 20-21 0.05212728045596293 1105 22-23 0.19998110660240087 1105 24-25 0.10013727161377517 1105 26-27 0.09709874666918239 1105 28-29 0.09504975350565559 1105 30-31 0.02735666623310351 1105 32-33 0.030803590814592496 1105 34-35 0.18267576257791518 1105 36-37 0.10602239905431787 1105 38-39 0.16502430243685495 1105 40-41 0.015012585988131377 1105 42-43 0.1148809819486516 1105 44-45 0.14673722660884891 1105 46-47 0.05306030226310554 1105 48-49 0.05838781758929912 1105 50-51 0.10200389423707179 1105 52-53 0.15781165108671757 1105 54-55 0.06276130785773404 1105 56-57 0.0057399498393309045 1105 58-59 0.05002391127680994 1105 60-61 0.2173870121075936 1105 62-63 0.22761353261666528 1105 64-65 0.21164902019398824 1105 66-67 0.05376820853904718 1105 68-69 0.0805668155065149 1105 70-71 0.02329385164099307 1105 72-73 0.14360398398358143 1105 74-75 0.24139502375905408 1105 76-77 0.09865914520798924 1105 78-79 0.022944437013535435 1105 80-81 0.36127127461368147 1105 82-83 0.42879066006222644 1105 84-85 0.3134334850262164 1105 86-87 0.472926934902798 1105 88-89 0.4242719619966415 1105 90-91 0.5189769225223664 1105 92-93 0.6909839452524515 1105 94-95 0.9025766998586349 1105 96-97 0.993994025719136 1105 98-99 1.0594501053241707 1105 100-101 1.1173695508018646 1106 1 -0.07879389769840728 1106 2 -0.017717952013317273 1106 3 0.008536941595028935 1106 4 0.1387198153538023 1106 5 0.1348894088817687 1106 6 -0.0921372007400123 1106 7 -0.2575913333917512 1106 8 -0.17871710840250188 1106 9 -0.43972766770579597 1106 10-11 -0.6859855981631213 1106 12-13 -0.20872394236970848 1106 14-15 -0.30609246911723886 1106 16-17 -0.2716143868543526 1106 18-19 -0.31414477224461024 1106 20-21 -0.19878115592822354 1106 22-23 -0.20563407623848207 1106 24-25 -0.28398875030914184 1106 26-27 -0.3576057385215847 1106 28-29 -0.04449079111359566 1106 30-31 -0.3816662564736859 1106 32-33 -0.214732162339331 1106 34-35 0.047113836782266105 1106 36-37 -0.15278021346966142 1106 38-39 -0.2518974519572552 1106 40-41 -0.11145034424492906 1106 42-43 -0.03110658906961561 1106 44-45 0.15328171719762906 1106 46-47 0.13737353765823457 1106 48-49 0.2856872469279992 1106 50-51 0.09585343226164866 1106 52-53 0.20915490351751487 1106 54-55 0.058212233308026384 1106 56-57 0.14478615995761146 1106 58-59 0.1499005815905221 1106 60-61 -0.03162214227084803 1106 62-63 0.006084200974328269 1106 64-65 0.0260669848514965 1106 66-67 0.1908143470707344 1106 68-69 0.1473130980820514 1106 70-71 0.20605982488502406 1106 72-73 0.04753349585003264 1106 74-75 0.2526278925533916 1106 76-77 0.3033783830428618 1106 78-79 0.20629495354955552 1106 80-81 0.08205190956748609 1106 82-83 0.02040814721775064 1106 84-85 -0.11088182299448235 1106 86-87 0.03562349574705337 1106 88-89 0.3752322986664929 1106 90-91 0.5187862869204132 1106 92-93 0.6459557314252073 1106 94-95 0.638595304635782 1106 96-97 0.8555068440739397 1106 98-99 0.8800587389584607 1106 100-101 1.1348036693628174 1107 1 0.009294702021854562 1107 2 -0.004133732737713558 1107 3 -0.03642312662990577 1107 4 -0.08035288581397282 1107 5 0.010260454070490255 1107 6 -0.03716457723165689 1107 7 0.11833022853082298 1107 8 0.03714493179500522 1107 9 0.045582702196128366 1107 10-11 0.20779981978671458 1107 12-13 0.20689353244248565 1107 14-15 0.12277242614553785 1107 16-17 0.1547425416227597 1107 18-19 0.07713203519091394 1107 20-21 0.21626378663057721 1107 22-23 0.3267803645003724 1107 24-25 0.2621584822756873 1107 26-27 0.1953374660728926 1107 28-29 0.16365526010041975 1107 30-31 0.17881861538827337 1107 32-33 0.24396620804873947 1107 34-35 0.32382883287869646 1107 36-37 0.1986757977198863 1107 38-39 0.17830730009907114 1107 40-41 0.23335105774455656 1107 42-43 0.22217642699991558 1107 44-45 0.21767955797727723 1107 46-47 0.379228696654053 1107 48-49 0.28845708252485025 1107 50-51 0.21957408596036032 1107 52-53 0.2390188913804039 1107 54-55 0.23586576001401482 1107 56-57 0.2600993313665825 1107 58-59 0.22555504762534184 1107 60-61 0.426523651451717 1107 62-63 0.44384954592018033 1107 64-65 0.5046093205668001 1107 66-67 0.4234035472610742 1107 68-69 0.2713039890642861 1107 70-71 0.5521477829365722 1107 72-73 0.5428534598836166 1107 74-75 0.6302812132662119 1107 76-77 0.5944767231499384 1107 78-79 0.6004673811584098 1107 80-81 0.7610634462147026 1107 82-83 0.790601270959904 1107 84-85 0.7816597824750495 1107 86-87 0.7579462540779325 1107 88-89 0.7397484052720706 1107 90-91 0.7166598452257489 1107 92-93 0.7602572189018559 1107 94-95 0.9214888082293307 1107 96-97 1.0917763696510825 1107 98-99 1.3748402883566513 1107 100-101 1.5555676259147404 1108 1 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0.8424017523884828 2310 70-71 0.9730917767269034 2310 72-73 1.1048822979456645 2310 74-75 0.9827181946778616 2310 76-77 1.0294216384077401 2310 78-79 1.01069167408075 2310 80-81 1.0695610672526463 2310 82-83 0.9573249100777979 2310 84-85 1.3820613891823008 2310 86-87 1.4345783325310997 2310 88-89 1.2877301223030422 2310 90-91 1.4420877765705313 2310 92-93 1.6296457245626002 2310 94-95 1.7134610767134326 2310 96-97 1.7229210868747025 2310 98-99 1.7339493560620767 2310 100-101 1.6775201723028914 2311 1 0.4637332477839742 2311 2 0.4903215858142538 2311 3 0.47614053571697923 2311 4 0.4020066510638074 2311 5 0.38669858976256677 2311 6 0.36176908648726425 2311 7 0.4503194433802946 2311 8 0.48408094288231496 2311 9 0.519186626946734 2311 10-11 0.7142148723378554 2311 12-13 0.5845311744061163 2311 14-15 0.5998415198650449 2311 16-17 0.7889810009515656 2311 18-19 0.7075001372911629 2311 20-21 0.6476794429816124 2311 22-23 0.7946465203095201 2311 24-25 0.7845152647982729 2311 26-27 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1.7420588189972293 2311 94-95 1.8692536952365337 2311 96-97 1.7742620364696826 2311 98-99 1.5493187979627194 2311 100-101 1.5586940246107375 2312 1 0.365397988942906 2312 2 0.47512347288839507 2312 3 0.4647729582047617 2312 4 0.4319704989862174 2312 5 0.3230540515054443 2312 6 0.3256807458768165 2312 7 0.4192646020107418 2312 8 0.47011565255172627 2312 9 0.5485389443316535 2312 10-11 0.6104536437184578 2312 12-13 0.6392978970388228 2312 14-15 0.6644963754749327 2312 16-17 0.6497932053784794 2312 18-19 0.8053447361845514 2312 20-21 0.752289062557935 2312 22-23 0.7222593921947293 2312 24-25 0.7209192470391912 2312 26-27 0.8304616533632156 2312 28-29 0.7619134865378427 2312 30-31 0.7658182294439513 2312 32-33 0.7641534938096797 2312 34-35 0.14042620845732046 2312 36-37 0.2496721164048452 2312 38-39 0.535480783212293 2312 40-41 0.6976700487171144 2312 42-43 0.6947763785856012 2312 44-45 0.8335684745242418 2312 46-47 0.5367650587748756 2312 48-49 0.45821120858933995 2312 50-51 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58-59 0.15765320284259587 2315 60-61 0.16776929803818064 2315 62-63 0.1464157152711394 2315 64-65 0.3838410666468235 2315 66-67 0.4747041216328327 2315 68-69 0.4342290647262317 2315 70-71 0.5499279856142678 2315 72-73 0.5722003464642142 2315 74-75 0.5910491630175514 2315 76-77 0.6652529614964777 2315 78-79 0.7123371184074081 2315 80-81 0.7851542354816026 2315 82-83 0.8215608849699123 2315 84-85 0.7426333628947006 2315 86-87 0.9166937636972641 2315 88-89 0.7030983031221076 2315 90-91 1.1393715465929866 2315 92-93 0.8462681867157436 2315 94-95 1.0539577323341902 2315 96-97 1.0196608388007782 2315 98-99 1.6045436859202589 2315 100-101 1.532355123570106 2316 1 -0.036031093819278937 2316 2 0.047084683499136304 2316 3 0.017140622224491864 2316 4 0.03797185117954882 2316 5 -0.05702827320654791 2316 6 -0.153052036857531 2316 7 0.1281453306043261 2316 8 -0.028366444143351544 2316 9 -0.05100303331738587 2316 10-11 -0.36443798196260957 2316 12-13 -0.11213880838187862 2316 14-15 0.0827831332599871 2316 16-17 0.14792565404152924 2316 18-19 0.11658782607889862 2316 20-21 0.25897545975809777 2316 22-23 0.13383986758348954 2316 24-25 0.3232650373431767 2316 26-27 0.21237838741860315 2316 28-29 0.2483004126842232 2316 30-31 0.0786388782316152 2316 32-33 0.19394715175778998 2316 34-35 0.12725392311801187 2316 36-37 0.04801468530704511 2316 38-39 0.09996184587717494 2316 40-41 0.2565318863743755 2316 42-43 0.12148617403321538 2316 44-45 0.137178963302361 2316 46-47 0.1050342513033442 2316 48-49 0.12126815979107164 2316 50-51 -0.21745970225232014 2316 52-53 -0.05214751675344331 2316 54-55 -0.10083702709830078 2316 56-57 -0.08823965706320536 2316 58-59 0.1009413638737513 2316 60-61 0.05564781838778998 2316 62-63 -0.10339300136462981 2316 64-65 0.06906221964408132 2316 66-67 -0.29582573328608675 2316 68-69 0.11931227298967428 2316 70-71 0.15595414729285295 2316 72-73 -0.16523196865306033 2316 74-75 0.009452094067256667 2316 76-77 -0.15374147657185233 2316 78-79 0.19805524029905186 2316 80-81 0.044839795059015586 2316 82-83 0.22949656961721487 2316 84-85 0.33708504177407406 2316 86-87 0.24780526718450702 2316 88-89 0.20379170531745316 2316 90-91 0.3227663113144459 2316 92-93 -0.048914047473950006 2316 94-95 0.12523837445772656 2316 96-97 0.415115283931879 2316 98-99 0.662519098650904 2316 100-101 0.57368704434937 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11921.0 3 3071.0 4 2313.0 5 2568.0 6 2807.0 7 3318.0 8 3980.0 9 5034.0 10 6128.0 11 7569.0 12 9389.0 13 11849.0 14 12945.0 15 15551.0 16 18802.0 17 23803.0 18 21507.0 19 16720.0 20 17223.0 21 17976.0 22 20093.0 23 22033.0 24 25157.0 25 28519.0 26 32855.0 27 38571.0 28 45522.0 29 54857.0 30 66650.0 31 82694.0 32 104350.0 33 135238.0 34 182721.0 35 255638.0 36 378190.0 37 523921.0 38 403888.0 39 43188.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15068518978707 18.856454615627403 14.701724390840806 23.291135803744723 2 14.994430213687536 19.840954798969353 40.7673676492486 24.39724733809451 3 19.034343629285416 24.025425262123097 29.88165635865255 27.058574749938934 4 12.661081320504 16.427929243904966 37.499938743391354 33.41105069219968 5 13.91629153212461 36.76833686038964 34.56823025709187 14.747141350393884 6 32.09396784984933 35.88691189024666 17.75362209546103 14.265498164442983 7 28.68451218316604 32.14704263165436 20.871524347478527 18.296920837701073 8 26.194785956686502 35.06051455753926 20.082666719953007 18.662032765821234 9 25.908135366760316 16.907084270196663 20.59029552854172 36.5944848345013 10-11 24.506821862386342 26.67637582551416 27.881763183246704 20.935039128852793 12-13 25.50622514828313 23.786817681272485 28.37590736869253 22.331049801751853 14-15 22.37209112070917 25.270673535397787 26.453650296785657 25.903585047107384 16-17 22.138141845244512 28.702946807844782 26.89570153814366 22.263209808767048 18-19 21.96719809591816 27.24259512209099 29.15572534682649 21.634481435164364 20-21 22.542220328903404 26.3800739085483 29.375094836109067 21.702610926439224 22-23 22.33161840630538 26.36599460678258 29.20103400788135 22.101352979030693 24-25 22.186608934815684 26.515894604941938 29.240999063604058 22.05649739663832 26-27 22.16794091700785 26.749606037986233 28.990552451612416 22.0919005933935 28-29 21.89489408703596 26.790909675318886 29.246354555389516 22.06784168225564 30-31 22.084749430858874 26.462705891845467 29.264241347509014 22.18830332978665 32-33 21.847206776633186 26.644450154520825 29.210308376173046 22.298034692672942 34-35 22.228186234030087 26.606399353247223 29.00858893114602 22.15682548157667 36-37 22.207808902756838 26.48792391275255 28.90768400826498 22.396583176225636 38-39 21.975472329747912 26.710211430613633 29.10665627181825 22.207659967820202 40-41 22.27489822141223 26.494814989061123 29.023131008866642 22.207155780660003 42-43 22.086521669058996 26.570847733662323 28.91051988211444 22.432110715164242 44-45 22.11587296859205 26.803537339145443 28.64991706769154 22.430672624570963 46-47 22.192854598967916 26.905292902920742 28.41120529740893 22.49064720070241 48-49 22.33597995725381 27.009874310502685 28.155435358071024 22.49871037417249 50-51 22.224304566625268 27.00302101246811 28.220419465026445 22.552254955880173 52-53 22.484713666954818 27.111422394609917 27.946701878412362 22.4571620600229 54-55 22.312336205433738 26.91681592790211 28.114576066667922 22.65627179999623 56-57 22.274482291683945 27.365934167014743 28.079107248142616 22.280476293158696 58-59 22.47195669794912 26.99344856901467 28.04013592480752 22.494458808228686 60-61 22.32914328488215 26.803784103546292 28.41484928395533 22.452223327616235 62-63 22.34294438741192 27.003608597837253 28.328180930839952 22.325266083910876 64-65 22.255352533460577 26.927612154489772 28.263151913784302 22.553883398265352 66-67 22.119833819079897 27.216431897012306 28.06804786417565 22.595686419732147 68-69 22.01543366231542 27.064392740080027 28.269202934604632 22.65097066299992 70-71 22.02793868430892 26.96664259350839 28.305416762155183 22.7000019600275 72-73 22.204805555173383 26.92632222124009 28.122443294739195 22.746428928847333 74-75 22.09299191654072 27.069684285400076 28.0328238693966 22.804499928662608 76-77 22.16719370390309 27.046774366235837 28.00686430186039 22.779167628000682 78-79 22.109154926259027 27.101337412200223 27.990884750334327 22.798622911206422 80-81 22.045065767401955 27.29211477235289 27.986033049938293 22.676786410306864 82-83 22.07211214261402 27.20611433411271 28.016203671419937 22.705569851853333 84-85 22.14824336065527 26.995667063082962 27.905074945296438 22.95101463096533 86-87 22.176709883911492 26.907065300467192 28.141439832204977 22.774784983416332 88-89 22.24019466022574 26.764499387917233 28.036708003064653 22.958597948792374 90-91 22.13920088909062 26.836840712388383 28.23313219718932 22.790826201331676 92-93 22.148251600634033 26.610821171907244 28.22385693903361 23.017070288425113 94-95 22.0169681115225 26.585542143696518 28.20792580244203 23.189563942338953 96-97 22.055083140415302 26.651734603595383 28.16531652317033 23.127865732818986 98-99 21.879176388403508 26.564365532027352 28.135639481994946 23.420818597574193 100-101 22.036111252978348 26.355783305344133 28.517306033512035 23.090799408165484 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3568.0 1 2100.0 2 563.5 3 468.5 4 374.5 5 325.0 6 493.0 7 942.5 8 1569.5 9 2014.0 10 2054.0 11 1986.5 12 2100.5 13 2301.0 14 2508.0 15 2703.5 16 2820.0 17 2979.0 18 3272.0 19 3539.0 20 3812.0 21 4464.5 22 5351.0 23 6398.0 24 7792.0 25 9770.5 26 12784.5 27 16571.0 28 20194.5 29 24493.0 30 30502.0 31 35392.0 32 39882.5 33 45171.5 34 51443.0 35 57661.5 36 63748.0 37 70006.0 38 73843.0 39 76459.0 40 79650.0 41 85935.5 42 92683.5 43 98228.0 44 103843.5 45 106771.5 46 106324.0 47 106844.0 48 111001.5 49 110957.5 50 106057.0 51 100404.0 52 93964.5 53 88969.0 54 84242.0 55 77402.5 56 69619.0 57 62241.0 58 55600.5 59 49722.0 60 42467.5 61 33481.0 62 25202.5 63 18973.0 64 14666.5 65 11427.5 66 8394.5 67 6285.5 68 4462.5 69 2861.0 70 2040.0 71 1511.0 72 1111.5 73 790.0 74 568.0 75 397.0 76 259.0 77 166.0 78 107.5 79 73.5 80 52.5 81 37.0 82 27.0 83 20.0 84 15.0 85 14.0 86 10.0 87 7.0 88 6.5 89 6.0 90 5.5 91 5.0 92 4.0 93 1.5 94 1.0 95 2.5 96 3.0 97 2.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2509249354162623 2 0.22038213110536364 3 0.2110914012226149 4 0.21774913073373445 5 0.21583080189154746 6 0.24479380597946862 7 0.24614792045630648 8 0.23422419020192858 9 0.22929671807787963 10-11 0.24182227698862996 12-13 0.24293189857381653 14-15 0.2279614107465534 16-17 0.24095714829509463 18-19 0.23159118983029936 20-21 0.22423759593524925 22-23 0.22467016028201692 24-25 0.21966745957592146 26-27 0.2265884891242039 28-29 0.23755305495748832 30-31 0.23779754784913962 32-33 0.238192497904884 34-35 0.24622314903835302 36-37 0.22286467431289975 38-39 0.22448208882690054 40-41 0.22077708116110803 42-43 0.22536602466594746 44-45 0.24076907683997828 46-47 0.24639241334795778 48-49 0.2494579780663546 50-51 0.24970247095800588 52-53 0.2699201523830158 54-55 0.2487809208279357 56-57 0.2223756885295972 58-59 0.20610750766203106 60-61 0.20358735016347168 62-63 0.21043315112970765 64-65 0.20328643583528552 66-67 0.20430202169291392 68-69 0.20808225794075297 70-71 0.20838317226893918 72-73 0.2111478226591498 74-75 0.21355513728463937 76-77 0.22568574613964532 78-79 0.2206642382880382 80-81 0.21552988756336128 82-83 0.21007581536498654 84-85 0.2117308441700106 86-87 0.21829453795357193 88-89 0.21577438045501254 90-91 0.22440686024485398 92-93 0.2147399874518725 94-95 0.21238909426291788 96-97 0.2212848740899222 98-99 0.22579858901271513 100-101 0.20527999325951904 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2658564.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.79160751679359 #Duplication Level Percentage of deduplicated Percentage of total 1 76.78221113902481 41.30238565862 2 12.983274464518125 13.967824085563349 3 4.229558514278161 6.825442547080905 4 1.8992725284226104 4.086596896653489 5 1.0636992738400715 2.860904692715174 6 0.6251603092720922 2.0177026794865003 7 0.43639351411525024 1.6432016043913305 8 0.2965810120201975 1.2762855516419123 9 0.2327101564788487 1.1266068062233665 >10 1.2720444190221118 12.771686193467884 >50 0.11371962070264047 4.236608712757834 >100 0.061949648345695274 5.807981483045264 >500 0.0027962448648577174 1.017328513299088 >1k 4.89342851346031E-4 0.5251346450850496 >5k 1.3981224324172313E-4 0.5343099299687412 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8081 0.30396108575907893 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6128 0.23050037539062443 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3846 0.14466456327551264 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3778 0.14210679148593 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 3.761429102327422E-5 0.0 0.0 0.0 3.761429102327422E-5 14-15 3.761429102327422E-5 0.0 0.0 0.0 3.761429102327422E-5 16-17 3.761429102327422E-5 0.0 0.0 0.0 3.761429102327422E-5 18-19 5.6421436534911335E-5 0.0 0.0 0.0 3.761429102327422E-5 20-21 7.522858204654844E-5 0.0 0.0 0.0 3.761429102327422E-5 22-23 1.1284287306982266E-4 0.0 0.0 3.761429102327422E-5 9.403572755818555E-5 24-25 1.5045716409309688E-4 0.0 0.0 1.5045716409309688E-4 1.5045716409309688E-4 26-27 1.5045716409309688E-4 0.0 0.0 2.6330003716291955E-4 1.5045716409309688E-4 28-29 1.69264309604734E-4 0.0 0.0 7.899001114887586E-4 1.880714551163711E-4 30-31 2.256857461396453E-4 0.0 0.0 0.002106400297303356 1.880714551163711E-4 32-33 2.256857461396453E-4 0.0 0.0 0.004250414885629987 1.880714551163711E-4 34-35 2.8210718267455666E-4 0.0 0.0 0.006526079492538077 1.880714551163711E-4 36-37 3.38528619209468E-4 0.0 0.0 0.008895779827004353 1.880714551163711E-4 38-39 3.761429102327422E-4 0.0 0.0 0.011472358762098636 1.880714551163711E-4 40-41 3.949500557443793E-4 0.0 0.0 0.015403052174030792 1.880714551163711E-4 42-43 4.889857833025649E-4 0.0 0.0 0.021496567319801214 1.880714551163711E-4 44-45 5.642143653491133E-4 0.0 0.0 0.03174646162364344 1.880714551163711E-4 46-47 5.830215108607504E-4 0.0 0.0 0.05572557215098076 1.880714551163711E-4 48-49 6.018286563723875E-4 0.0 0.0 0.0966499207843031 1.880714551163711E-4 50-51 6.394429473956618E-4 0.0 0.0 0.13012663979501715 1.880714551163711E-4 52-53 6.394429473956618E-4 0.0 0.0 0.16174145140007914 1.880714551163711E-4 54-55 6.394429473956618E-4 0.0 0.0 0.20163140703026144 1.880714551163711E-4 56-57 6.394429473956618E-4 0.0 0.0 0.23725214062930214 1.880714551163711E-4 58-59 6.394429473956618E-4 0.0 0.0 0.28650805472427976 1.880714551163711E-4 60-61 6.394429473956618E-4 0.0 0.0 0.3574486075941749 1.880714551163711E-4 62-63 6.394429473956618E-4 0.0 0.0 0.4158824086988314 1.880714551163711E-4 64-65 6.77057238418936E-4 0.0 0.0 0.4818202608626311 1.880714551163711E-4 66-67 6.77057238418936E-4 0.0 0.0 0.5953213840253611 1.880714551163711E-4 68-69 7.146715294422102E-4 0.0 0.0 0.7191100157829565 1.880714551163711E-4 70-71 7.710929659771216E-4 0.0 0.0 0.8310877601592439 1.880714551163711E-4 72-73 7.899001114887586E-4 0.0 0.0 0.9630763073599131 1.880714551163711E-4 74-75 8.087072570003957E-4 0.0 0.0 1.0926575399350926 1.880714551163711E-4 76-77 8.275144025120328E-4 0.0 0.0 1.2118572281878488 1.880714551163711E-4 78-79 8.275144025120328E-4 0.0 0.0 1.357161234410757 1.880714551163711E-4 80-81 8.275144025120328E-4 0.0 0.0 1.5442359108150114 1.880714551163711E-4 82-83 8.65128693535307E-4 0.0 0.0 1.7429710174364808 1.880714551163711E-4 84-85 9.403572755818555E-4 3.761429102327422E-5 0.0 1.975803478870548 1.880714551163711E-4 86-87 0.001015585857628404 3.761429102327422E-5 0.0 2.314087605188365 1.880714551163711E-4 88-89 0.001015585857628404 3.761429102327422E-5 0.0 2.7630893971331894 1.880714551163711E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4270 0.0 49.74674 1 GTATCAA 8045 0.0 45.069576 1 ATCAACG 9920 0.0 36.303143 3 TATCAAC 10145 0.0 35.590317 2 TCAACGC 10175 0.0 35.44203 4 CAACGCA 10420 0.0 34.516205 5 AACGCAG 10620 0.0 33.955017 6 ACGCAGA 12215 0.0 29.052885 7 CGCAGAG 12260 0.0 28.634628 8 GTACATG 9045 0.0 26.059021 1 GCAGAGT 13370 0.0 25.758444 9 TACATGG 9260 0.0 24.472466 2 ACATGGG 9195 0.0 24.026358 3 GAGTACT 7555 0.0 22.540926 12-13 TGGTATC 2195 0.0 21.643913 2 AGAGTAC 12180 0.0 20.82625 10-11 CAGAGTA 12985 0.0 20.650906 10-11 GTACTTT 8295 0.0 20.443182 14-15 AGTACTT 8105 0.0 20.014963 12-13 GTGGTAT 2720 0.0 19.742153 1 >>END_MODULE