FastQCFastQC Report
Thu 26 May 2016
SRR937444_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937444_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658564
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90690.34112400529007386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72430.2724403098815752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47470.17855503948748272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45850.052.662151
GTATCAA84150.042.3059961
ATCAACG104900.033.5076943
TCAACGC105700.033.2540864
TATCAAC107150.032.98142
CAACGCA108400.032.42585
AACGCAG110550.031.8375436
ACGCAGA128400.027.3036357
CGCAGAG129500.026.924988
GTACATG87350.025.4113791
GCAGAGT142200.023.8855679
TACATGG92950.023.5580732
ACATGGG88850.023.0948223
GAGTACT85900.022.4247112-13
GTACTTT90350.021.21267714-15
AGAGTAC129850.020.74293710-11
CAGAGTA138850.020.44189510-11
AGTACTT92750.019.92346212-13
CATGGGG50650.019.8812964
CTACGGT1759.943952E-518.9997674
Produced by FastQC (version 0.11.5)