FastQCFastQC Report
Thu 26 May 2016
SRR937443_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937443_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227686
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65550.5339313146846995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45800.37305956083233005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32850.26757656273672586No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18970.1545183377508581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21900.059.679971
GTATCAA46400.046.4002461
ATCAACG58100.036.2978443
TATCAAC58950.036.0161862
TCAACGC58750.035.900654
CAACGCA59250.035.524775
TGGTATC9450.034.6809772
GTGGTAT9900.034.565061
AACGCAG61800.034.2099086
ACGCAGA72000.028.901617
CGCAGAG72450.028.4597938
GCAGAGT77350.026.2269619
GTACATG46550.025.8309781
TACATGG46850.025.2443262
CCGTCTA950.001225172725.0049959
ACATGGG48250.023.2321133
GAGTACT51550.022.80543112-13
AGTACTT52200.021.88448312-13
GTACTTT52500.021.80466814-15
GACCGTC1754.042591E-621.7186267
Produced by FastQC (version 0.11.5)