##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937443_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1227686 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.200358234923264 33.0 31.0 34.0 31.0 34.0 2 32.350702052479214 34.0 31.0 34.0 31.0 34.0 3 32.395087994813004 34.0 31.0 34.0 31.0 34.0 4 35.97686541998524 37.0 35.0 37.0 35.0 37.0 5 35.982177853294736 37.0 35.0 37.0 35.0 37.0 6 36.043588507158994 37.0 37.0 37.0 35.0 37.0 7 35.99948765400925 37.0 37.0 37.0 35.0 37.0 8 35.99149212420765 37.0 37.0 37.0 35.0 37.0 9 37.68444781483213 39.0 38.0 39.0 35.0 39.0 10-11 37.5838097037842 39.0 38.0 39.0 35.0 39.0 12-13 37.47213090317882 39.0 37.0 39.0 35.0 39.0 14-15 38.703362667652804 40.0 38.0 41.0 35.5 41.0 16-17 38.62317033834384 40.0 38.0 41.0 35.0 41.0 18-19 38.54195168797233 40.0 38.0 41.0 35.0 41.0 20-21 38.42208105329864 40.0 38.0 41.0 34.0 41.0 22-23 38.495022342846624 40.0 38.0 41.0 35.0 41.0 24-25 38.471131461953625 40.0 38.0 41.0 34.5 41.0 26-27 38.385153044019404 40.0 38.0 41.0 34.0 41.0 28-29 38.24417440615923 40.0 38.0 41.0 34.0 41.0 30-31 37.99007278734139 40.0 38.0 41.0 34.0 41.0 32-33 37.752143870664 40.0 37.0 41.0 33.5 41.0 34-35 37.75819183406832 40.0 38.0 41.0 33.5 41.0 36-37 37.6403270054395 40.0 37.0 41.0 33.0 41.0 38-39 37.511653224032855 40.0 37.0 41.0 33.0 41.0 40-41 37.2776096656637 40.0 37.0 41.0 32.5 41.0 42-43 36.99825851235576 40.0 37.0 41.0 32.0 41.0 44-45 36.742804348994774 39.0 36.0 41.0 31.0 41.0 46-47 36.308438395485496 39.0 36.0 41.0 30.5 41.0 48-49 35.949174300268965 39.0 36.0 40.0 29.5 41.0 50-51 35.0048917231279 38.0 34.0 39.5 27.5 40.5 52-53 35.1247326270724 38.0 35.0 39.5 28.0 40.5 54-55 35.42557135945185 39.0 35.0 40.0 28.0 41.0 56-57 35.158715665080486 38.0 35.0 40.0 27.0 41.0 58-59 34.88637363299736 38.0 34.5 40.0 26.0 41.0 60-61 34.5583504251087 38.0 34.0 40.0 26.0 41.0 62-63 34.16716285760366 37.5 34.0 40.0 25.5 41.0 64-65 33.7444525717488 37.0 33.5 39.5 23.5 41.0 66-67 33.29573685779589 36.5 33.0 39.0 22.5 40.5 68-69 32.78683067168641 36.0 32.0 39.0 21.0 40.0 70-71 32.31797096325934 35.0 32.0 38.5 19.5 40.0 72-73 31.810607109635527 35.0 31.0 38.0 17.5 40.0 74-75 31.243047082071474 35.0 31.0 37.0 12.0 39.0 76-77 30.70608445481988 34.5 30.0 36.5 7.5 39.0 78-79 30.140351441655277 34.0 29.5 36.0 4.5 38.5 80-81 29.541414905765805 34.0 29.0 35.5 2.0 37.0 82-83 28.959892431778158 34.0 29.0 35.0 2.0 37.0 84-85 28.348136249822836 33.5 27.5 35.0 2.0 36.0 86-87 27.69886844030151 33.0 26.0 35.0 2.0 36.0 88-89 27.096123927453764 33.0 25.5 35.0 2.0 35.5 90-91 26.50080558058005 32.0 25.0 35.0 2.0 35.0 92-93 25.787803233074257 32.0 23.5 34.0 2.0 35.0 94-95 24.939486155254684 31.5 19.0 34.0 2.0 35.0 96-97 24.012105701294956 31.0 8.5 34.0 2.0 35.0 98-99 22.74931334233672 31.0 2.0 34.0 2.0 35.0 100-101 19.557764770470627 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15638597629330775 1101 2 0.12343044416913784 1101 3 0.06452036700203223 1101 4 0.060237812158305815 1101 5 0.06598975792500994 1101 6 0.07489270857839614 1101 7 0.073985955848066 1101 8 0.036197138777801285 1101 9 0.09716517681331283 1101 10-11 0.12686792953712 1101 12-13 0.22289934586859061 1101 14-15 0.28725909658609083 1101 16-17 0.31152202962928754 1101 18-19 0.3294557583219202 1101 20-21 0.2851114707496265 1101 22-23 0.32159285625156286 1101 24-25 0.2654368612518212 1101 26-27 0.24970715390866616 1101 28-29 0.2403778963619274 1101 30-31 0.2835811443540379 1101 32-33 0.11948073008625926 1101 34-35 0.2856668203426054 1101 36-37 0.3764961652734655 1101 38-39 0.5127886665665358 1101 40-41 0.6457310335265305 1101 42-43 0.6366940388623519 1101 44-45 0.6501896023097302 1101 46-47 0.7175099672404954 1101 48-49 0.8004292347783988 1101 50-51 0.8001879801145222 1101 52-53 0.7075105699461446 1101 54-55 0.9043063023277327 1101 56-57 0.9721269241102277 1101 58-59 0.9114201516703204 1101 60-61 0.9125573074141755 1101 62-63 1.0002279678175299 1101 64-65 0.8102637137428346 1101 66-67 1.0004598776474936 1101 68-69 0.9990628668461881 1101 70-71 1.143333189255543 1101 72-73 1.1029698336333347 1101 74-75 1.1595566871115714 1101 76-77 1.2004523927225144 1101 78-79 1.3441714163418261 1101 80-81 1.2060243570378084 1101 82-83 0.9490235837253316 1101 84-85 1.3032466225534307 1101 86-87 1.564939436450814 1101 88-89 1.6862507994426537 1101 90-91 1.58689093614856 1101 92-93 1.9344949949596852 1101 94-95 1.948036433595071 1101 96-97 1.9714689296441108 1101 98-99 2.1060671598726017 1101 100-101 2.1161883584192402 1102 1 0.24618382651021875 1102 2 0.2444535099308638 1102 3 0.16553312608525061 1102 4 0.13702439447102677 1102 5 0.1683420197292662 1102 6 0.24471899353247295 1102 7 0.13491231426463912 1102 8 0.1787728581437591 1102 9 0.21412222135497672 1102 10-11 0.21270849033739125 1102 12-13 0.30366149185062596 1102 14-15 0.48573575488314447 1102 16-17 0.4941201897775329 1102 18-19 0.5456173246446596 1102 20-21 0.5400711029254737 1102 22-23 0.4986402121499438 1102 24-25 0.47766672211165684 1102 26-27 0.4794711602096342 1102 28-29 0.538831163615562 1102 30-31 0.6459266812333126 1102 32-33 0.5450405618169398 1102 34-35 0.582062406745095 1102 36-37 0.7520055600667632 1102 38-39 0.7000495022846565 1102 40-41 0.667163990775741 1102 42-43 0.8443452857146099 1102 44-45 0.8431035780926877 1102 46-47 0.9716454306207254 1102 48-49 0.891729228565147 1102 50-51 0.7548066147354575 1102 52-53 0.7496308949354571 1102 54-55 0.9690190858984451 1102 56-57 0.9199119019435571 1102 58-59 1.014366326686492 1102 60-61 1.0225609988132192 1102 62-63 1.001095081106925 1102 64-65 0.9375731224245669 1102 66-67 1.2140712720095195 1102 68-69 1.1220293993798407 1102 70-71 1.232082097368945 1102 72-73 1.3504333673621183 1102 74-75 1.207010352743918 1102 76-77 1.1926693885084703 1102 78-79 1.3474443349958172 1102 80-81 1.637482203819161 1102 82-83 1.5778077245978928 1102 84-85 1.678462715591305 1102 86-87 1.729132683017994 1102 88-89 1.7999997989273169 1102 90-91 1.762752293048372 1102 92-93 1.9615143912514732 1102 94-95 2.092096022278195 1102 96-97 1.9016283907729488 1102 98-99 2.1464459485820377 1102 100-101 1.8847330700332563 1103 1 0.2864103227830981 1103 2 0.2901789398113479 1103 3 0.308299627179629 1103 4 0.13658740576212836 1103 5 0.24176566406243438 1103 6 0.3382654272402732 1103 7 0.28777789793716124 1103 8 0.21123374065740563 1103 9 0.3681435670130426 1103 10-11 0.21406207399464705 1103 12-13 0.22531393253115795 1103 14-15 0.3918235737115694 1103 16-17 0.355293106909329 1103 18-19 0.30678486687894946 1103 20-21 0.3127630297021966 1103 22-23 0.31752715674651455 1103 24-25 0.36409227532855226 1103 26-27 0.4370727600844475 1103 28-29 0.2772584367996629 1103 30-31 0.399869438892857 1103 32-33 0.37073486181764537 1103 34-35 0.5814912046190486 1103 36-37 0.5451055592398575 1103 38-39 0.7191052631888653 1103 40-41 0.6817261465358229 1103 42-43 0.7550119447482828 1103 44-45 0.8931535741807792 1103 46-47 0.8193712446950698 1103 48-49 0.9192479087193064 1103 50-51 0.8039538506705597 1103 52-53 0.7453880653349714 1103 54-55 0.7335274926834146 1103 56-57 0.7152283307471592 1103 58-59 0.6756313803750373 1103 60-61 0.7730940359865173 1103 62-63 0.8417502016032259 1103 64-65 0.7084496696158453 1103 66-67 0.8247575859938578 1103 68-69 1.0227787364228078 1103 70-71 1.1618379116404753 1103 72-73 1.105376205804145 1103 74-75 0.9681677176574759 1103 76-77 1.058085930343939 1103 78-79 1.1180627916941788 1103 80-81 1.1602600017395552 1103 82-83 1.162884401168366 1103 84-85 1.0697266306920952 1103 86-87 1.4376438809331695 1103 88-89 1.5766712171306025 1103 90-91 1.6015918749385918 1103 92-93 1.7522659736658248 1103 94-95 1.8060306189986797 1103 96-97 1.6556942304050892 1103 98-99 1.9238517240843365 1103 100-101 1.7701642672105784 1104 1 0.23354621332541114 1104 2 0.3450332767213027 1104 3 0.17274438268091075 1104 4 0.23004765028498042 1104 5 0.29544774574060284 1104 6 0.2614149742503429 1104 7 0.196725876898995 1104 8 0.2454981529128304 1104 9 0.38938767637419147 1104 10-11 0.3381856579486211 1104 12-13 0.3689666836948078 1104 14-15 0.4887909304417448 1104 16-17 0.4957650337616428 1104 18-19 0.5532324637235533 1104 20-21 0.4661378086775443 1104 22-23 0.4611898831811416 1104 24-25 0.4592965833584799 1104 26-27 0.4325491355858233 1104 28-29 0.43058414387743227 1104 30-31 0.4638831661528613 1104 32-33 0.6211854383722155 1104 34-35 0.7331730835739663 1104 36-37 0.7723462544840629 1104 38-39 0.8117555800646059 1104 40-41 0.8866676061753935 1104 42-43 0.842534328773688 1104 44-45 0.9010900284867986 1104 46-47 0.8941423363597565 1104 48-49 0.8278473967626567 1104 50-51 0.8863159626606745 1104 52-53 0.9345081016320833 1104 54-55 1.1298783133103356 1104 56-57 1.1500956033310885 1104 58-59 1.3297855176186744 1104 60-61 1.2963574941555436 1104 62-63 1.2644753169221872 1104 64-65 1.1870324410357327 1104 66-67 1.3348581914284523 1104 68-69 1.437067507646411 1104 70-71 1.3262583725301766 1104 72-73 1.4030553463862923 1104 74-75 1.1873178245698561 1104 76-77 1.3597497090087636 1104 78-79 1.4150425370673432 1104 80-81 1.5540413856314252 1104 82-83 1.690399738147498 1104 84-85 1.806473920073099 1104 86-87 1.617651346297258 1104 88-89 1.9542112509499887 1104 90-91 1.9521796919148926 1104 92-93 2.0674456009446516 1104 94-95 1.8959198774016386 1104 96-97 1.7041787132275132 1104 98-99 1.885642092155745 1104 100-101 1.7053213053273772 1105 1 0.15735280743312785 1105 2 0.015308359444688335 1105 3 0.17538697370918754 1105 4 0.11856761376136404 1105 5 0.16405647918168142 1105 6 0.07414257545933367 1105 7 0.09854538448122696 1105 8 0.19708710923911354 1105 9 0.22295294443377145 1105 10-11 0.19302235148716562 1105 12-13 0.23449988128529498 1105 14-15 0.31280325472067005 1105 16-17 0.17613131159176731 1105 18-19 0.22332151038231274 1105 20-21 0.20883984010022516 1105 22-23 0.24034212513544873 1105 24-25 0.23167991205311722 1105 26-27 0.41160518566419313 1105 28-29 0.4102713819336117 1105 30-31 0.43394991452564824 1105 32-33 0.5431889637800538 1105 34-35 0.5367888238851961 1105 36-37 0.5747329848880156 1105 38-39 0.6294954127786312 1105 40-41 0.6238526835764873 1105 42-43 0.6451319977076224 1105 44-45 0.5518967544310911 1105 46-47 0.7299764957062465 1105 48-49 0.8108938634608975 1105 50-51 0.7942564311199973 1105 52-53 0.7979000127253997 1105 54-55 0.9086407421633496 1105 56-57 0.8846229529112506 1105 58-59 0.7274339436363846 1105 60-61 0.9584447113782844 1105 62-63 0.9539565481130978 1105 64-65 0.967503537086003 1105 66-67 0.860854574874331 1105 68-69 0.7059688857455839 1105 70-71 0.8579473293447393 1105 72-73 0.9797205970304184 1105 74-75 1.0416760001468894 1105 76-77 1.214134251902042 1105 78-79 0.9765729481858898 1105 80-81 1.177559729633753 1105 82-83 1.3814109247255573 1105 84-85 1.396705154926135 1105 86-87 1.220139965219552 1105 88-89 1.745096297589214 1105 90-91 1.6946405112222784 1105 92-93 1.7227388396811705 1105 94-95 1.7764621001012948 1105 96-97 1.9405854665156923 1105 98-99 2.0808704217211584 1105 100-101 1.8567800526845382 1106 1 0.07551436107644349 1106 2 -0.5047095412360108 1106 3 -0.08089244484025926 1106 4 0.08334730988747907 1106 5 0.07479782551116898 1106 6 0.056006077409513466 1106 7 0.05663153016722333 1106 8 0.1554153250084198 1106 9 0.2180994839258048 1106 10-11 -0.14266811322933393 1106 12-13 0.07412130511293213 1106 14-15 0.10214846407779277 1106 16-17 0.06672746946613017 1106 18-19 0.22946772339236787 1106 20-21 0.07737501685826942 1106 22-23 0.18233909613304178 1106 24-25 0.11897552026760394 1106 26-27 0.1836113437127338 1106 28-29 0.344619027623402 1106 30-31 0.4243544261403329 1106 32-33 0.37608244713728567 1106 34-35 0.4727121582353462 1106 36-37 0.5956463662274558 1106 38-39 0.537853797983594 1106 40-41 0.5328367886831415 1106 42-43 0.3631790816787017 1106 44-45 0.5375304309352487 1106 46-47 0.618245967051088 1106 48-49 0.6106384839892485 1106 50-51 0.5847657653334792 1106 52-53 0.6686834935413444 1106 54-55 0.8600746501079328 1106 56-57 0.6134752057485358 1106 58-59 0.24968875946883884 1106 60-61 0.3220927075337414 1106 62-63 0.6025931620617797 1106 64-65 0.7448044199272061 1106 66-67 0.8718015467875091 1106 68-69 0.9535592388508007 1106 70-71 0.7948853929001984 1106 72-73 0.7813388042736698 1106 74-75 0.5862850056006117 1106 76-77 0.6532446934744947 1106 78-79 0.7125374612476207 1106 80-81 0.8339742965359846 1106 82-83 0.7496263188643333 1106 84-85 0.6381823451802653 1106 86-87 0.7776644368524686 1106 88-89 0.9028898153811404 1106 90-91 0.9378773991299845 1106 92-93 1.3791250459740105 1106 94-95 1.5039374118558513 1106 96-97 1.507771080228114 1106 98-99 1.4327233074243289 1106 100-101 1.044527733478958 1107 1 0.2116553760656501 1107 2 0.32146932320154775 1107 3 0.1398779027556074 1107 4 0.2361950032531368 1107 5 0.21608482523521388 1107 6 0.24339713127500318 1107 7 0.24849964999204843 1107 8 0.19480057137539575 1107 9 0.3533509109936688 1107 10-11 0.32033944431865535 1107 12-13 0.3552066517436856 1107 14-15 0.24696518280164526 1107 16-17 0.2646671996376213 1107 18-19 0.34988569148864457 1107 20-21 0.387113289428612 1107 22-23 0.4445110729287194 1107 24-25 0.36594749034940577 1107 26-27 0.32452735269155397 1107 28-29 0.3573615600462219 1107 30-31 0.27964014331751486 1107 32-33 0.31453722995590283 1107 34-35 0.44772001988220467 1107 36-37 0.4807490761136819 1107 38-39 0.6514041177070169 1107 40-41 0.54458927195153 1107 42-43 0.49332204270319124 1107 44-45 0.4918770947583937 1107 46-47 0.5108260040464927 1107 48-49 0.47724966248590306 1107 50-51 0.44856452297111815 1107 52-53 0.5045609397230066 1107 54-55 0.5752764340229746 1107 56-57 0.5947780620563918 1107 58-59 0.46362132554493485 1107 60-61 0.5366741988721131 1107 62-63 0.5942778352320204 1107 64-65 0.448046133603313 1107 66-67 0.5259976915444895 1107 68-69 0.8130075561128081 1107 70-71 0.7530643935898524 1107 72-73 0.8435712059092815 1107 74-75 0.8910137923596722 1107 76-77 0.7979345397811493 1107 78-79 0.7820724318769052 1107 80-81 0.7151797523461241 1107 82-83 0.7206712687250487 1107 84-85 1.053359495112364 1107 86-87 0.8171666822967829 1107 88-89 0.919707815664502 1107 90-91 1.043568540325161 1107 92-93 0.9752531170146632 1107 94-95 0.6415063586222551 1107 96-97 0.7337545512698576 1107 98-99 1.0557132805540341 1107 100-101 0.7757551821536346 1108 1 0.11853460079431244 1108 2 0.05714068266286887 1108 3 -0.030743978709644182 1108 4 0.012710770297495344 1108 5 0.033734122722940185 1108 6 -0.014631304063826178 1108 7 -0.01967341008852941 1108 8 0.034739764124672945 1108 9 -0.0547970073569104 1108 10-11 0.1265838649860953 1108 12-13 0.06769436753943125 1108 14-15 0.18322519922044478 1108 16-17 0.19815135521245253 1108 18-19 0.10140253131246624 1108 20-21 -0.12323358062297984 1108 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sequence quality scores pass #Quality Count 2 5123.0 3 749.0 4 615.0 5 673.0 6 776.0 7 832.0 8 1007.0 9 1294.0 10 1585.0 11 2138.0 12 3148.0 13 4335.0 14 4983.0 15 6876.0 16 9268.0 17 13399.0 18 11233.0 19 6891.0 20 6853.0 21 7199.0 22 7911.0 23 8913.0 24 10075.0 25 11857.0 26 14046.0 27 16628.0 28 20547.0 29 24417.0 30 30765.0 31 39424.0 32 51729.0 33 69614.0 34 98817.0 35 139803.0 36 198515.0 37 241221.0 38 143863.0 39 10564.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.11913616600354 19.100389377119132 15.110887121476349 23.669587335400973 2 14.570377821906355 19.912158366429765 41.30785887855351 24.209604933110366 3 18.0413819007259 24.54564012117352 30.547975406021116 26.865002572079465 4 12.016719169234639 16.51871230715717 38.06770953260467 33.396858991003526 5 13.120228890763288 37.07880123035318 34.86509636323997 14.935873515643564 6 30.921646082875576 36.57629064002815 17.98206193831312 14.520001338783162 7 27.52579137354268 33.037202597177846 21.36288164620717 18.074124383072306 8 25.78345985209591 34.474845773369914 20.36060908600364 19.381085288530535 9 25.672581030982037 16.463529628834383 20.863094970817336 37.00079436936624 10-11 23.930409351473028 26.659568857053976 28.22024329048793 21.189778500985064 12-13 24.829576533403 24.192988758174202 28.791564956853264 22.185869751569527 14-15 21.922032482859986 25.37187205225862 27.24307940222917 25.463016062652223 16-17 21.201843212642903 28.771504487626885 27.712065369560868 22.31458693016934 18-19 20.935659436093662 26.93981560079924 30.716154845702793 21.408370117404306 20-21 21.511543908481315 26.08641708261741 30.912942155978467 21.48909685292281 22-23 21.27057821005385 26.030675602766372 30.862601345312342 21.836144841867437 24-25 21.13179717634325 26.248384333369927 30.85346786236541 21.766350627921412 26-27 20.907735240895263 26.5552879258937 30.694954695676234 21.8420221375348 28-29 20.803630245844833 26.47384863037237 30.951657944509613 21.770863179273185 30-31 20.92664042230455 26.356766032763545 30.869917331892125 21.84667621303978 32-33 20.746773138240666 26.413755861780153 30.851504562688447 21.987966437290737 34-35 21.23937331324052 26.57422721313456 30.41991637428142 21.7664830993435 36-37 21.07328122767994 26.436544701344815 30.470379364528704 22.019794706446543 38-39 20.90040670479337 26.648182805765202 30.58778351192813 21.8636269775133 40-41 21.129853356158655 26.488240131075557 30.593777991793498 21.788128520972293 42-43 20.983002660591534 26.52808517810944 30.383922664493245 22.10498949680578 44-45 21.040592035372587 26.777414142749805 30.126488669157013 22.05550515272059 46-47 21.083159357647073 26.884416880437378 29.744271046976493 22.288152714939052 48-49 21.317595159854516 27.00259468434606 29.505279368361503 22.174530787437924 50-51 21.31271808151942 27.15420047253377 29.23631260378517 22.296768842161637 52-53 21.399295224969546 27.25696202118244 29.14443152610901 22.199311227739006 54-55 21.26337882262279 27.1336114882157 29.24185515319879 22.361154535962715 56-57 21.305550091697594 27.431088054990806 29.124004364919692 22.13935748839191 58-59 21.384910932588195 27.057959920480773 29.248778322196507 22.30835082473453 60-61 21.373033156770887 27.054165539433573 29.506983948608777 22.065817355186763 62-63 21.199082475585666 27.13205879436378 29.53625515046478 22.132603579585776 64-65 21.179464622345105 27.04977566106132 29.40374317149484 22.367016545098735 66-67 21.044163301548334 27.31883040782949 29.20193648005902 22.435069810563153 68-69 21.07968197072381 27.1493173894308 29.37339222522558 22.397608414619814 70-71 21.16442534680778 27.197360608675957 29.281614648050553 22.35659939646571 72-73 21.320987014492136 27.131145835076175 29.118494544584966 22.429372605846723 74-75 21.0692324392796 27.33462780952754 28.993654944556262 22.602484806636596 76-77 21.143095025283753 27.19952330227452 29.12198645819304 22.53539521424869 78-79 21.25957675360909 27.209611934353223 29.08325184351997 22.44755946851772 80-81 21.106806925440186 27.375845192970083 29.02799135462681 22.48935652696292 82-83 21.129283752885623 27.359581213535638 28.978469371057198 22.53266566252154 84-85 21.192668261776447 27.12410193469625 28.899912845638852 22.78331695788845 86-87 21.146688983708206 27.05616215164136 29.061268358208103 22.73588050644233 88-89 21.262373328458004 27.054127598725447 28.947303985582952 22.7361950872336 90-91 21.094811963094923 27.15197655338516 29.12924407764447 22.623967405875447 92-93 21.190434117948087 26.934272819687425 29.15915944102575 22.716133621338734 94-95 21.11961457724615 26.857046550423913 28.968530555346984 23.054808316982957 96-97 21.268182511976114 26.72507870484113 28.99672447469569 23.010014308487065 98-99 21.176935361238456 26.809640861306 28.78937459774989 23.22404917970566 100-101 21.487468747870448 26.023684492945293 29.497521660730104 22.991325098454155 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1925.0 1 1095.5 2 299.5 3 337.0 4 282.5 5 264.0 6 459.5 7 976.5 8 1657.5 9 1992.0 10 1751.0 11 1475.5 12 1481.0 13 1609.5 14 1767.5 15 1883.5 16 1960.0 17 2056.5 18 2216.0 19 2367.5 20 2571.5 21 2900.5 22 3408.5 23 4159.0 24 5064.0 25 6229.5 26 7858.0 27 9988.5 28 11817.0 29 13946.0 30 16612.0 31 19063.0 32 21771.0 33 24836.0 34 28257.0 35 30953.0 36 33538.0 37 36248.5 38 37662.0 39 38218.0 40 38727.5 41 40156.0 42 42051.5 43 44078.0 44 45766.0 45 46695.0 46 45988.0 47 44912.0 48 45378.0 49 45071.5 50 43497.5 51 41364.5 52 38862.5 53 37385.5 54 36056.0 55 33229.0 56 29655.5 57 25877.0 58 22830.5 59 20260.0 60 17282.0 61 14155.5 62 11273.0 63 8721.0 64 6708.5 65 5338.5 66 3989.0 67 2886.5 68 2088.5 69 1377.0 70 1002.0 71 769.0 72 595.5 73 468.5 74 359.0 75 279.5 76 197.0 77 116.0 78 62.5 79 44.5 80 35.0 81 27.0 82 15.5 83 12.5 84 9.5 85 4.5 86 4.5 87 4.5 88 4.0 89 4.5 90 4.5 91 2.5 92 1.0 93 1.0 94 1.5 95 2.0 96 1.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2788172219932458 2 0.24289598480393193 3 0.24403634154010068 4 0.2430588929090989 5 0.24411779559268412 6 0.21935576360730674 7 0.2169935960823859 8 0.22017030413314154 9 0.2292931580224911 10-11 0.23580948222916934 12-13 0.227745531023405 14-15 0.22004812305426633 16-17 0.21593469339880067 18-19 0.20510130440519808 20-21 0.21023290971795722 22-23 0.207137655719785 24-25 0.20954055027099763 26-27 0.20583439087844937 28-29 0.2154459690832998 30-31 0.22709389860273718 32-33 0.21955939873876543 34-35 0.220251758185725 36-37 0.21267653129546155 38-39 0.20143587203894156 40-41 0.19862570722481154 42-43 0.21035509079683243 44-45 0.208237285429662 46-47 0.21654559879317672 48-49 0.21841904200259674 50-51 0.2280713472337389 52-53 0.23605384438691981 54-55 0.2206590284486424 56-57 0.20062133151310677 58-59 0.18913631009883636 60-61 0.1906432100716307 62-63 0.1981369829093107 64-65 0.18799595336266767 66-67 0.1930461046228433 68-69 0.19467518567451286 70-71 0.19577481538438982 72-73 0.19838134506706112 74-75 0.20200605040702588 76-77 0.21104745024379196 78-79 0.21047727187570764 80-81 0.20542712061553198 82-83 0.19805552885672723 84-85 0.1852265155748294 86-87 0.18852540470446028 88-89 0.20119150988119114 90-91 0.19829989101447765 92-93 0.2018024152755672 94-95 0.1982591639881859 96-97 0.20681183950945112 98-99 0.2074227449038272 100-101 0.19300537759655156 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1227686.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.55887129305456 #Duplication Level Percentage of deduplicated Percentage of total 1 79.16999234663305 47.15275384445232 2 12.078568319830566 14.3877179193031 3 3.7344205634777756 6.672536210829273 4 1.6703979173259036 3.9794805826479958 5 0.8562805882507725 2.54995526731844 6 0.538721996818525 1.9251404442751128 7 0.3706828740434368 1.5454217509984827 8 0.24228367438979015 1.1544113743511881 9 0.1872177286237002 1.0035428942589277 >10 1.0378774667354511 11.374367028125059 >50 0.07943117820578753 3.2523742936030366 >100 0.03303333837001452 3.423564712930853 >500 4.0950273571874424E-4 0.1609708368234544 >1k 5.460036476249922E-4 0.8848518121143826 >5k 1.3650091190624806E-4 0.5329110279685573 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6555 0.5339313146846995 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4580 0.37305956083233005 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3285 0.26757656273672586 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1897 0.1545183377508581 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.145405258347819E-5 2 0.0 0.0 0.0 0.0 8.145405258347819E-5 3 0.0 0.0 0.0 0.0 8.145405258347819E-5 4 0.0 0.0 0.0 0.0 8.145405258347819E-5 5 0.0 0.0 0.0 0.0 8.145405258347819E-5 6 0.0 0.0 0.0 0.0 1.6290810516695638E-4 7 0.0 0.0 0.0 0.0 1.6290810516695638E-4 8 0.0 0.0 0.0 8.145405258347819E-5 2.4436215775043454E-4 9 0.0 0.0 0.0 8.145405258347819E-5 2.4436215775043454E-4 10-11 0.0 0.0 0.0 8.145405258347819E-5 2.4436215775043454E-4 12-13 0.0 0.0 0.0 8.145405258347819E-5 2.4436215775043454E-4 14-15 0.0 0.0 0.0 8.145405258347819E-5 2.4436215775043454E-4 16-17 0.0 0.0 0.0 2.0363513145869546E-4 2.4436215775043454E-4 18-19 0.0 0.0 0.0 2.4436215775043454E-4 2.4436215775043454E-4 20-21 0.0 0.0 0.0 3.2581621033391276E-4 2.4436215775043454E-4 22-23 0.0 0.0 0.0 3.6654323662565184E-4 2.850891840421736E-4 24-25 0.0 0.0 0.0 6.516324206678255E-4 4.072702629173909E-4 26-27 0.0 0.0 0.0 0.0011810837624604336 4.072702629173909E-4 28-29 0.0 0.0 0.0 0.0020363513145869546 4.072702629173909E-4 30-31 0.0 0.0 0.0 0.004357791813216083 4.072702629173909E-4 32-33 0.0 0.0 0.0 0.00851194849497347 4.887243155008691E-4 34-35 0.0 0.0 0.0 0.013399191649982161 4.887243155008691E-4 36-37 0.0 0.0 0.0 0.0197933347777852 4.887243155008691E-4 38-39 0.0 0.0 0.0 0.02924200487746867 4.887243155008691E-4 40-41 0.0 0.0 0.0 0.046836080235499955 4.887243155008691E-4 42-43 0.0 0.0 0.0 0.06504106098790732 5.294513417926081E-4 44-45 0.0 0.0 0.0 0.0881332848953234 5.701783680843473E-4 46-47 0.0 0.0 0.0 0.1243803382949712 5.701783680843473E-4 48-49 0.0 0.0 0.0 0.15989430522136766 5.701783680843473E-4 50-51 0.0 0.0 0.0 0.19752607751493462 5.701783680843473E-4 52-53 0.0 0.0 0.0 0.2343840363089585 6.923594469595646E-4 54-55 0.0 0.0 0.0 0.27559978691619846 8.145405258347818E-4 56-57 0.0 0.0 0.0 0.33954121819422883 8.145405258347818E-4 58-59 0.0 0.0 0.0 0.4038899197351766 8.145405258347818E-4 60-61 0.0 0.0 0.0 0.4657135456460365 8.145405258347818E-4 62-63 0.0 0.0 0.0 0.5369858416565799 8.145405258347818E-4 64-65 0.0 0.0 0.0 0.6165664510306381 8.145405258347818E-4 66-67 0.0 0.0 0.0 0.7111346060800563 8.145405258347818E-4 68-69 0.0 0.0 0.0 0.8242742851185075 8.145405258347818E-4 70-71 0.0 0.0 0.0 0.9566371205666595 8.145405258347818E-4 72-73 0.0 0.0 0.0 1.1002406152713315 8.145405258347818E-4 74-75 0.0 0.0 0.0 1.263474536648622 8.145405258347818E-4 76-77 0.0 0.0 0.0 1.4492305035652437 8.145405258347818E-4 78-79 0.0 0.0 0.0 1.6545354431019006 8.145405258347818E-4 80-81 0.0 0.0 0.0 1.889896928041861 8.55267552126521E-4 82-83 0.0 0.0 0.0 2.136051074949132 9.774486310017382E-4 84-85 0.0 0.0 0.0 2.4064785295262796 9.774486310017382E-4 86-87 0.0 0.0 0.0 2.6849699353091916 9.774486310017382E-4 88-89 0.0 0.0 0.0 2.978530340820047 9.774486310017382E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2190 0.0 59.67997 1 GTATCAA 4640 0.0 46.400246 1 ATCAACG 5810 0.0 36.297844 3 TATCAAC 5895 0.0 36.016186 2 TCAACGC 5875 0.0 35.90065 4 CAACGCA 5925 0.0 35.52477 5 TGGTATC 945 0.0 34.680977 2 GTGGTAT 990 0.0 34.56506 1 AACGCAG 6180 0.0 34.209908 6 ACGCAGA 7200 0.0 28.90161 7 CGCAGAG 7245 0.0 28.459793 8 GCAGAGT 7735 0.0 26.226961 9 GTACATG 4655 0.0 25.830978 1 TACATGG 4685 0.0 25.244326 2 CCGTCTA 95 0.0012251727 25.004995 9 ACATGGG 4825 0.0 23.232113 3 GAGTACT 5155 0.0 22.805431 12-13 AGTACTT 5220 0.0 21.884483 12-13 GTACTTT 5250 0.0 21.804668 14-15 GACCGTC 175 4.042591E-6 21.718626 7 >>END_MODULE