FastQCFastQC Report
Thu 26 May 2016
SRR937443_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937443_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227686
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57720.4701527915118361No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49640.4043379170243857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36490.2972258378771119No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13590.11069605746094685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28750.058.1852381
GTATCAA52150.041.09891
GTGGTAT12050.033.1283841
TGGTATC12450.030.520992
TATCAAC70950.029.9918732
ATCAACG72450.029.1742423
TCAACGC72700.029.0085834
CAACGCA74100.028.4605125
AACGCAG77550.027.194386
TATACCG1056.907906E-527.141885
GGACCGT950.0012268624.99916
GTGTAGG6150.023.1821731
ACGCAGA91000.022.9158767
CGCAGAG90950.022.7717868
TGCACCG2755.2750693E-1122.4537375
GCACCGT2806.548362E-1122.0527766
AGTACTT62900.021.56099512-13
GCAGAGT95250.021.1453199
GAGTACT61300.020.92264712-13
AGGCCCG3950.020.4439688
Produced by FastQC (version 0.11.5)