##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937443_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1227686 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.124692307316366 31.0 31.0 33.0 30.0 34.0 2 31.386666460316402 31.0 31.0 34.0 30.0 34.0 3 31.384412626681414 31.0 31.0 34.0 28.0 34.0 4 35.221883282858975 37.0 35.0 37.0 33.0 37.0 5 35.067729859263686 35.0 35.0 37.0 32.0 37.0 6 35.18353064220004 37.0 35.0 37.0 32.0 37.0 7 35.11092657242976 37.0 35.0 37.0 32.0 37.0 8 35.07097417417809 37.0 35.0 37.0 32.0 37.0 9 36.551858537117795 38.0 35.0 39.0 32.0 39.0 10-11 36.3942885232869 38.0 35.0 39.0 32.0 39.0 12-13 36.21357456222519 38.0 35.0 39.0 32.0 39.0 14-15 37.063270657155 38.0 36.0 40.0 31.5 41.0 16-17 36.95357729908136 38.0 36.0 40.0 31.0 41.0 18-19 36.89696754707637 38.5 36.0 40.0 31.0 41.0 20-21 36.73119918285295 38.0 35.5 40.0 31.0 41.0 22-23 36.620059200805414 38.0 35.0 40.0 30.5 41.0 24-25 36.63014443432604 38.0 35.0 40.0 30.0 41.0 26-27 36.31543448406188 38.0 35.0 40.0 30.0 41.0 28-29 36.04686010918101 38.0 34.0 40.0 29.0 41.0 30-31 36.05355400322232 38.0 35.0 40.0 30.0 41.0 32-33 35.870340624557095 38.0 34.5 40.0 29.5 41.0 34-35 35.62212121014657 38.0 34.0 40.0 29.0 41.0 36-37 35.33638813181872 38.0 34.0 40.0 27.0 41.0 38-39 35.10881283976522 38.0 34.0 40.0 27.0 41.0 40-41 34.74905920569266 38.0 33.0 40.0 26.0 41.0 42-43 34.40940761725718 37.0 33.0 40.0 25.0 41.0 44-45 33.879263916017614 37.0 32.5 39.5 24.0 40.5 46-47 33.33051529462745 37.0 31.5 39.0 22.5 40.0 48-49 32.82560809522956 36.5 31.0 39.0 19.5 40.0 50-51 32.394679910009565 36.0 31.0 39.0 18.0 40.0 52-53 31.949703751610755 36.0 30.0 39.0 14.5 40.0 54-55 31.559796234542056 35.0 30.0 39.0 12.5 40.0 56-57 31.023980073080576 35.0 28.5 38.0 8.5 40.0 58-59 30.56598552072761 34.5 28.0 38.0 8.0 40.0 60-61 29.96862023351248 34.0 27.5 38.0 6.5 40.0 62-63 29.344909854800008 34.0 26.5 38.0 2.0 39.5 64-65 28.66034148797005 33.0 26.0 37.0 2.0 39.0 66-67 27.95174580470902 32.5 25.0 36.5 2.0 39.0 68-69 27.264280117228672 32.0 23.0 36.0 2.0 39.0 70-71 26.61126053404535 31.0 22.0 35.5 2.0 38.0 72-73 25.892089263867145 31.0 20.5 35.0 2.0 38.0 74-75 25.198676208737414 30.5 19.0 34.5 2.0 37.0 76-77 24.417749326782257 29.5 19.5 33.5 2.0 36.0 78-79 24.91668757320683 30.5 19.0 34.0 2.0 36.0 80-81 24.53882018692076 30.0 17.5 34.0 2.0 36.0 82-83 23.841125499516977 30.0 10.5 34.0 2.0 35.5 84-85 23.046243502002955 29.0 7.0 34.0 2.0 35.0 86-87 22.238426193668413 29.0 2.0 34.0 2.0 35.0 88-89 21.323343265297478 28.5 2.0 33.0 2.0 35.0 90-91 20.252448916090923 26.5 2.0 33.0 2.0 34.5 92-93 19.210023980073082 25.5 2.0 32.0 2.0 34.0 94-95 18.15776428174631 24.5 2.0 32.0 2.0 34.0 96-97 16.923907253157566 19.0 2.0 32.0 2.0 34.0 98-99 15.439348905176079 2.0 2.0 31.0 2.0 34.0 100-101 13.121158830515295 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19243029238813492 1101 2 0.19922151400531973 1101 3 0.13618437136488382 1101 4 0.21231364555431753 1101 5 0.2333870069032642 1101 6 0.2710557368660673 1101 7 0.13433518001975386 1101 8 0.11579218673737302 1101 9 0.10360140691600606 1101 10-11 0.2603052744270684 1101 12-13 0.16338412208196473 1101 14-15 0.5153548738133509 1101 16-17 0.4252011973963832 1101 18-19 0.3086900077381429 1101 20-21 0.31724498102014564 1101 22-23 0.36431534922994047 1101 24-25 0.27348481058846374 1101 26-27 0.10506481785467514 1101 28-29 -0.023920896165996908 1101 30-31 0.4463880368586146 1101 32-33 0.3219686510320159 1101 34-35 0.2964290275686565 1101 36-37 0.49321545968388847 1101 38-39 0.29605356091224877 1101 40-41 0.49836748920905904 1101 42-43 0.48205567499626767 1101 44-45 0.6182924371676606 1101 46-47 0.7037252557337013 1101 48-49 0.57227477900026 1101 50-51 0.6388798508033986 1101 52-53 0.5460376442837145 1101 54-55 0.34291370239893837 1101 56-57 0.5821627958350533 1101 58-59 0.844111514622913 1101 60-61 0.7569406192565324 1101 62-63 0.7922738032690795 1101 64-65 0.5451242162797136 1101 66-67 0.4743550863985355 1101 68-69 0.9094117338811749 1101 70-71 0.9756172765489843 1101 72-73 0.9430894151159173 1101 74-75 1.1970300501012154 1101 76-77 1.2872279407415625 1101 78-79 1.3179244486354307 1101 80-81 1.2933318989906475 1101 82-83 1.2785729026161832 1101 84-85 1.5362304671470284 1101 86-87 1.754913271283801 1101 88-89 2.168681168892732 1101 90-91 2.2842130724769056 1101 92-93 2.2591427419392325 1101 94-95 2.3458242777256224 1101 96-97 2.469872084892522 1101 98-99 2.735372122843893 1101 100-101 2.743164951478221 1102 1 0.06795339287094038 1102 2 0.32641031261940157 1102 3 0.30013182923359594 1102 4 0.2740787921686447 1102 5 0.2283835165210064 1102 6 0.2759422720312088 1102 7 0.2744822512052778 1102 8 0.14102892968077185 1102 9 0.3438365599994242 1102 10-11 0.3372362233359141 1102 12-13 0.24099944175346621 1102 14-15 0.35282092938899723 1102 16-17 0.5564587583684286 1102 18-19 0.41590687560432826 1102 20-21 0.4864499413832917 1102 22-23 0.49913273462296814 1102 24-25 0.3025801619840678 1102 26-27 0.1864418924392055 1102 28-29 0.0769018106894066 1102 30-31 0.36978054222210943 1102 32-33 0.6632942287725214 1102 34-35 0.73659546946201 1102 36-37 0.6002779101149827 1102 38-39 0.56272242048923 1102 40-41 0.7070402667858176 1102 42-43 0.9246599021963959 1102 44-45 0.9474035044205706 1102 46-47 1.0092725878755502 1102 48-49 1.1570627428464846 1102 50-51 1.0772124714408733 1102 52-53 1.1826952469210568 1102 54-55 1.266184683982143 1102 56-57 1.3171897869062548 1102 58-59 1.1570251980178305 1102 60-61 1.1843041197261819 1102 62-63 1.471529602603166 1102 64-65 1.5848433044676007 1102 66-67 1.6606117519321977 1102 68-69 1.7639614745695305 1102 70-71 1.7113615320376603 1102 72-73 1.8433878336620886 1102 74-75 1.6634209582950241 1102 76-77 1.7064959472764336 1102 78-79 1.566437838180363 1102 80-81 1.975679701877251 1102 82-83 2.026751873337176 1102 84-85 2.4689373590986783 1102 86-87 2.6963954593697217 1102 88-89 2.857983238634592 1102 90-91 3.15426668913425 1102 92-93 3.4306995986215796 1102 94-95 3.396094005695101 1102 96-97 3.1662316710172433 1102 98-99 3.2590062138277407 1102 100-101 3.2667542705430197 1103 1 0.12206811544671936 1103 2 0.21277352974681207 1103 3 0.33759454684538426 1103 4 0.23539352390401547 1103 5 0.0972681394162862 1103 6 0.14524352899393023 1103 7 0.12888486474124505 1103 8 0.053254945075238425 1103 9 0.08582335110928341 1103 10-11 0.2835759817390411 1103 12-13 0.16083968326129394 1103 14-15 0.335152146475707 1103 16-17 0.23696833045661236 1103 18-19 0.16537161214211693 1103 20-21 0.43518391448845506 1103 22-23 0.3487819032948849 1103 24-25 0.3251580709644699 1103 26-27 0.36810611336849064 1103 28-29 0.23807447338818832 1103 30-31 0.398896584669167 1103 32-33 0.28089635023502524 1103 34-35 0.32157864189827023 1103 36-37 0.5407847915740049 1103 38-39 0.5482795302064716 1103 40-41 0.6199907801998492 1103 42-43 0.7907589606034051 1103 44-45 0.8878378724693761 1103 46-47 0.9271133039009527 1103 48-49 0.8137204916358556 1103 50-51 0.6670203299862933 1103 52-53 0.7296188972970157 1103 54-55 0.7086713723646731 1103 56-57 0.8171007353498538 1103 58-59 0.7993289490602145 1103 60-61 0.8544431610098435 1103 62-63 0.8260508455339028 1103 64-65 0.9510411146898328 1103 66-67 1.1631462228355538 1103 68-69 1.2043761240594257 1103 70-71 1.2620698735605522 1103 72-73 1.019855578234239 1103 74-75 0.8676098162915054 1103 76-77 0.8695200872451245 1103 78-79 1.3189185601086102 1103 80-81 1.5597807868432518 1103 82-83 1.5734352435314953 1103 84-85 1.5948694585590921 1103 86-87 1.6292381822355502 1103 88-89 1.7925192132370888 1103 90-91 2.0674504858219116 1103 92-93 2.3306552636681417 1103 94-95 2.221140614643634 1103 96-97 2.0827218247774795 1103 98-99 2.2398524962114994 1103 100-101 2.2993528649278066 1104 1 0.19903280137852875 1104 2 0.2578675466758149 1104 3 0.238142382292029 1104 4 0.035006419033344116 1104 5 0.17912939626825164 1104 6 0.14571622812527352 1104 7 0.24178079853118817 1104 8 0.2330685362210616 1104 9 0.1875666101590241 1104 10-11 0.11637113311378045 1104 12-13 0.25615806334163693 1104 14-15 0.2270636497329548 1104 16-17 0.24243687054352847 1104 18-19 0.1467810857535028 1104 20-21 0.37486763404175605 1104 22-23 0.25097951984868416 1104 24-25 -0.02196221310550328 1104 26-27 0.20053217971690884 1104 28-29 0.09651942517705692 1104 30-31 0.26335099365849857 1104 32-33 0.34945799660523136 1104 34-35 0.2974579539907083 1104 36-37 0.35867801434272195 1104 38-39 0.5108495783291076 1104 40-41 0.5405234274919692 1104 42-43 0.450231526194834 1104 44-45 0.5092756951573278 1104 46-47 0.7540058300096177 1104 48-49 0.9791231949342958 1104 50-51 0.6866971955630774 1104 52-53 0.7826398759354518 1104 54-55 1.0236141674034265 1104 56-57 0.9561188469023492 1104 58-59 1.092338858365693 1104 60-61 1.1783696529161993 1104 62-63 1.2767409894834998 1104 64-65 1.300874833358229 1104 66-67 1.4319426098567618 1104 68-69 1.5365104941773744 1104 70-71 1.7478575257839921 1104 72-73 1.5549701410036327 1104 74-75 1.85848968345951 1104 76-77 1.8427150860947528 1104 78-79 1.978283971982723 1104 80-81 2.072256472120234 1104 82-83 1.8721756242328844 1104 84-85 1.9838712396276357 1104 86-87 2.2509195946052074 1104 88-89 2.5614227539756094 1104 90-91 2.822376258543411 1104 92-93 2.6971187244114283 1104 94-95 2.774978117479346 1104 96-97 2.9734266420289046 1104 98-99 2.748049272911844 1104 100-101 2.7349192109377807 1105 1 0.14003283881360673 1105 2 0.2097380798978179 1105 3 0.16124942871315184 1105 4 0.17556862880975643 1105 5 0.11598813744048897 1105 6 0.06884931634021996 1105 7 0.15177873146379994 1105 8 0.09298679754069639 1105 9 0.17186959340926933 1105 10-11 0.26354551710328167 1105 12-13 0.23525643133497454 1105 14-15 0.44749115833081277 1105 16-17 0.32566904778398253 1105 18-19 0.4279261759801827 1105 20-21 0.4591035596729043 1105 22-23 0.3964226128325379 1105 24-25 0.2384510050579962 1105 26-27 0.2384561930177611 1105 28-29 0.2884616058843221 1105 30-31 0.3586844669664728 1105 32-33 0.48498421173975004 1105 34-35 0.475309408343513 1105 36-37 0.5990121956619632 1105 38-39 0.5860779729990071 1105 40-41 0.4768787482373469 1105 42-43 0.30978634172941355 1105 44-45 0.6568763507594824 1105 46-47 1.0011973039263182 1105 48-49 1.2632889704833232 1105 50-51 1.255889590708243 1105 52-53 1.3065185732598223 1105 54-55 1.505123517221211 1105 56-57 1.5139610889705892 1105 58-59 1.2321051658183642 1105 60-61 1.4252242111253075 1105 62-63 1.5469895421294275 1105 64-65 1.5107967568051173 1105 66-67 1.6273579520540729 1105 68-69 1.7380352412057611 1105 70-71 1.7818176525424256 1105 72-73 1.7941729691424406 1105 74-75 1.8932237397781435 1105 76-77 2.1783381441500502 1105 78-79 2.050399395723396 1105 80-81 2.068029400698986 1105 82-83 2.0895591726551572 1105 84-85 2.1792896742879613 1105 86-87 2.5877978671228163 1105 88-89 3.0500880632137743 1105 90-91 3.1639212205142506 1105 92-93 3.009030078145255 1105 94-95 3.2881471270936515 1105 96-97 3.3445653357399756 1105 98-99 3.3257879337451453 1105 100-101 3.264757247256318 1106 1 -0.14681708354084932 1106 2 0.09292095312678583 1106 3 0.011861370271539329 1106 4 0.15154250310571626 1106 5 0.057127144176021716 1106 6 0.11108697288743485 1106 7 0.0057546432573900574 1106 8 -0.001066808923916085 1106 9 0.008100696199278445 1106 10-11 -0.13755657171142843 1106 12-13 -0.3629367075413512 1106 14-15 -0.16263442780705617 1106 16-17 -0.2658091027112661 1106 18-19 -0.11415114263362369 1106 20-21 0.09824740870334381 1106 22-23 0.0995678714076007 1106 24-25 0.22799978552946243 1106 26-27 0.31137645593623375 1106 28-29 0.1514162186369461 1106 30-31 0.24977191098776785 1106 32-33 0.1964802182533063 1106 34-35 -0.39436733877051466 1106 36-37 0.2779915352929905 1106 38-39 0.23976657172139682 1106 40-41 0.05465046185682354 1106 42-43 0.4340446671314524 1106 44-45 0.29239964419889475 1106 46-47 0.5527615813493512 1106 48-49 0.5847133787403322 1106 50-51 0.6629987692286718 1106 52-53 0.5628821803899058 1106 54-55 0.8772118093810413 1106 56-57 0.8163526252714952 1106 58-59 0.6996157429857988 1106 60-61 0.4819697709034436 1106 62-63 0.7992875330817206 1106 64-65 0.8045907855346393 1106 66-67 0.7499905669335227 1106 68-69 1.033662437199009 1106 70-71 1.1307788726471024 1106 72-73 0.8893444749032184 1106 74-75 1.0096500707504745 1106 76-77 0.8760299031822321 1106 78-79 1.0228596808212131 1106 80-81 1.2721628143650463 1106 82-83 1.3117013499728323 1106 84-85 1.4582962133065784 1106 86-87 1.7245393268947424 1106 88-89 1.9931893114825492 1106 90-91 2.0198524511726497 1106 92-93 1.8388237692272007 1106 94-95 1.3840926318477393 1106 96-97 1.6652883213662832 1106 98-99 1.4905731233436352 1106 100-101 1.6096070722467921 1107 1 0.010339829006536405 1107 2 0.12859826670949204 1107 3 0.11735850033847584 1107 4 0.06453961959680754 1107 5 0.03201282324212684 1107 6 0.10467522598260359 1107 7 0.05071092164016022 1107 8 0.04855511620951347 1107 9 0.10636757948702069 1107 10-11 0.16903111810970017 1107 12-13 0.1851526284123679 1107 14-15 0.3533191615715978 1107 16-17 0.3476402621121011 1107 18-19 0.1776894799396942 1107 20-21 0.22569420744567736 1107 22-23 0.2267554949786188 1107 24-25 0.0898014133470042 1107 26-27 0.03556075762205779 1107 28-29 0.04230668034887941 1107 30-31 0.30320840097595436 1107 32-33 0.447411397618815 1107 34-35 0.4731842753477693 1107 36-37 0.4887784552898111 1107 38-39 0.670739584427892 1107 40-41 0.7916533206823786 1107 42-43 0.8466769637147351 1107 44-45 0.7849734116234899 1107 46-47 0.9094426482822016 1107 48-49 0.9134042676716732 1107 50-51 1.2739469702321031 1107 52-53 1.2911268930929936 1107 54-55 1.282972014588541 1107 56-57 1.2056231187459616 1107 58-59 1.2499599029299553 1107 60-61 1.5538590394222496 1107 62-63 1.3508848666875615 1107 64-65 1.6385318229363328 1107 66-67 1.6429517148475092 1107 68-69 1.8884683100590287 1107 70-71 1.937901944322121 1107 72-73 1.9411928381749881 1107 74-75 1.789800165263017 1107 76-77 1.8375214093947854 1107 78-79 1.7429277871195659 1107 80-81 1.8832245587768597 1107 82-83 2.1309133389719577 1107 84-85 2.1974013530217427 1107 86-87 2.4992235404610064 1107 88-89 2.7812405462177487 1107 90-91 2.7579709974023423 1107 92-93 2.8886155158062152 1107 94-95 2.7054871226228308 1107 96-97 2.5692476293833835 1107 98-99 2.8076964072954613 1107 100-101 2.804966651879475 1108 1 0.14197584935347152 1108 2 0.24971331017288634 1108 3 -0.007513733385348331 1108 4 -0.018810069226802284 1108 5 0.10141778588477735 1108 6 -0.06093589603216287 1108 7 0.06511078770100198 1108 8 0.1343838049353394 1108 9 0.24895931620091716 1108 10-11 0.09950304962627854 1108 12-13 0.22365310078706813 1108 14-15 -0.01338324096198562 1108 16-17 -0.018044934445718752 1108 18-19 0.32041557788551955 1108 20-21 0.0988863981739101 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0.02592327979186848 2316 16-17 -0.004349085218088078 2316 18-19 0.031387600773307156 2316 20-21 0.28293292912530177 2316 22-23 0.3469281579246015 2316 24-25 -0.058310976748892074 2316 26-27 0.21413693196263495 2316 28-29 0.07812379106134415 2316 30-31 0.3684165543655382 2316 32-33 0.2139634539605879 2316 34-35 0.30317549962965273 2316 36-37 0.39648046290835026 2316 38-39 0.2888269761848079 2316 40-41 0.3826259581594016 2316 42-43 0.6377200560895488 2316 44-45 0.8758699190537769 2316 46-47 0.557573032752785 2316 48-49 0.44387012819036187 2316 50-51 0.6536566517593556 2316 52-53 0.9395569531111505 2316 54-55 0.8575849971146177 2316 56-57 1.045370754561965 2316 58-59 0.4740217039177068 2316 60-61 1.4969234537468772 2316 62-63 1.3907846241787603 2316 64-65 1.7804043175657043 2316 66-67 1.8748389925477689 2316 68-69 2.1254659901688733 2316 70-71 2.2920838369981347 2316 72-73 1.864844090583464 2316 74-75 1.871936872680024 2316 76-77 2.057364221611028 2316 78-79 2.483828862352663 2316 80-81 2.175418599039965 2316 82-83 2.4495487286470627 2316 84-85 2.3962426537105124 2316 86-87 2.5481945390657508 2316 88-89 2.4944524313638077 2316 90-91 3.467221351753107 2316 92-93 2.940554202050759 2316 94-95 2.892299183836922 2316 96-97 3.146561844385708 2316 98-99 3.4474787860964273 2316 100-101 2.8582023172837676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 17.0 4 47.0 5 92.0 6 186.0 7 474.0 8 1024.0 9 1771.0 10 2767.0 11 4239.0 12 6157.0 13 8495.0 14 11130.0 15 15091.0 16 19219.0 17 24197.0 18 22323.0 19 19259.0 20 19894.0 21 21273.0 22 23280.0 23 25498.0 24 29169.0 25 33308.0 26 39033.0 27 45440.0 28 52860.0 29 59787.0 30 68245.0 31 77056.0 32 86038.0 33 96171.0 34 106457.0 35 111074.0 36 105023.0 37 71770.0 38 19337.0 39 478.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.50810406510954 23.579639661807132 16.22955855190097 19.682697721182354 2 18.811243265786203 24.34099598757337 36.01938932267697 20.828371423963457 3 21.757354893678023 26.187885175851157 30.669894419257044 21.38486551121378 4 16.31736453783785 20.041851092217392 35.565690249786996 28.07509412015776 5 13.55596029564852 38.90332449819741 34.0474751440529 13.493240062101178 6 31.044420153035873 38.81204151550152 16.340741850929312 13.802796480533297 7 27.45987166099475 34.40309655726301 20.67808869694694 17.458943084795298 8 26.430455344444752 36.463313909256925 19.21843207465101 17.88779867164731 9 26.192528056848413 16.814397166702236 20.358137178399037 36.634937598050314 10-11 24.98150993006355 28.038236161363738 27.058262454731913 19.921991453840803 12-13 25.651232901038014 25.353464756975413 28.01240272948023 20.98289961250634 14-15 22.57250632490718 26.64203224603034 26.483970656992096 24.301490772070384 16-17 21.584509394095885 30.113807602269638 27.038469119954122 21.263213883680354 18-19 21.539017305728013 28.028176585869673 30.16422766081881 20.2685784475835 20-21 22.17700157369486 27.060994523020582 30.57745315569805 20.184550747586513 22-23 21.934864027863984 27.137205358408313 30.46491018003092 20.463020433696784 24-25 21.907230350431625 27.332721884911944 30.461860768958836 20.2981869956976 26-27 21.699411889285308 27.551968786146002 30.32333870941466 20.425280615154033 28-29 21.513438440578835 27.501904067577087 30.46956139243342 20.515096099410655 30-31 21.652372803077018 27.347694803951832 30.525917521053884 20.474014871917262 32-33 21.6426709326956 27.471371896747005 30.37065334835892 20.515303822198476 34-35 21.994589821827407 27.422728613016684 30.17530541197016 20.407376153185748 36-37 22.070432533413484 27.17617826389576 30.162081412544172 20.591307790146583 38-39 21.798416614026962 27.546020540276807 30.100135580325745 20.555427265370486 40-41 21.96909470336878 27.34001202261816 30.127369702024787 20.563523571988277 42-43 22.00786102682117 27.50365558112539 29.73117732114127 20.757306070912165 44-45 22.0986473740028 27.784506787566205 29.338079932490878 20.77876590594012 46-47 22.20384528291436 28.01237449966848 28.828340471423473 20.955439745993683 48-49 22.35258038293179 28.196949382822645 28.576851084071986 20.873619150173578 50-51 22.278660830212285 28.30972251862447 28.3702021526677 21.041414498495545 52-53 22.65860325848792 28.39569727112633 28.05143171788226 20.894267752503488 54-55 22.370174458289824 28.378917736294134 28.1942206720611 21.056687133354945 56-57 22.331361602233795 28.604708370055537 28.10828664658553 20.955643381125142 58-59 22.54244977950388 28.270868935542147 28.215887450048307 20.970793834905667 60-61 22.442383475905075 28.371464690482746 28.357576774517263 20.828575059094913 62-63 22.327859077972708 28.348250285496455 28.420988754453504 20.902901882077337 64-65 22.287132051680967 28.204361701607738 28.29355388918665 21.214952357524645 66-67 22.23903343363042 28.615867575259475 28.040964872125286 21.10413411898482 68-69 22.310916635035344 28.349064826022293 28.08747513615045 21.25254340279192 70-71 22.332909229232882 28.498818101697015 27.97392818684908 21.194344482221027 72-73 22.49098913809324 28.4977824116121 27.784285056834612 21.226943393460047 74-75 22.467837867337416 28.686406784796763 27.4438659396621 21.401889408203726 76-77 22.328755072551125 28.512828198741374 27.883962185770628 21.274454542936873 78-79 22.36259923139956 28.482812380364358 27.79924997108381 21.355338417152268 80-81 22.251536630701988 28.61761883739002 27.693563337856748 21.43728119405125 82-83 22.35614664214893 28.57917254664785 27.675233621070593 21.38944719013263 84-85 22.273171752863416 28.453174693355916 27.66983075062791 21.60382280315276 86-87 22.40613642250543 28.334688185741303 27.74031796403966 21.5188574277136 88-89 22.41016839810831 28.27066530041069 27.64481308738554 21.67435321409546 90-91 22.329145250720835 28.463782731826807 27.630673757637545 21.576398259814813 92-93 22.36780531552288 28.24471752757361 27.57511494348841 21.812362213415103 94-95 22.271900143847855 28.20623514481716 27.505648838546666 22.01621587278832 96-97 22.501193301870348 28.198497009821725 27.38334557859257 21.91696410971535 98-99 22.326782372478228 28.16237097132198 27.195752973590487 22.31509368260931 100-101 22.750750995387108 27.771615938413284 27.599930755679562 21.877702310520046 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 359.0 1 255.5 2 164.5 3 202.0 4 209.5 5 224.0 6 361.5 7 695.5 8 1123.0 9 1377.5 10 1409.5 11 1406.0 12 1490.5 13 1639.5 14 1798.0 15 2010.0 16 2222.5 17 2433.0 18 2620.0 19 2769.5 20 2989.5 21 3391.5 22 3974.5 23 4635.5 24 5533.5 25 6812.0 26 8498.5 27 10564.0 28 12558.0 29 14854.0 30 17347.5 31 19911.5 32 22881.0 33 25872.0 34 29098.0 35 31639.5 36 34313.5 37 37077.0 38 38609.0 39 39440.5 40 39846.5 41 40850.5 42 42562.0 43 44275.5 44 45725.0 45 46495.5 46 45766.0 47 44554.5 48 44811.5 49 44534.0 50 42825.5 51 40771.0 52 38210.5 53 35823.0 54 33885.0 55 31286.0 56 27898.5 57 24670.0 58 21924.0 59 19385.5 60 16619.0 61 13795.0 62 11186.5 63 8764.5 64 6719.0 65 5230.0 66 3987.0 67 3047.5 68 2171.0 69 1461.5 70 1091.0 71 759.5 72 562.5 73 455.5 74 353.0 75 277.0 76 201.0 77 128.5 78 74.0 79 42.0 80 27.5 81 16.5 82 11.0 83 7.0 84 3.0 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027612923825799104 2 0.0 3 0.0 4 0.0 5 9.774486310017382E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004317064786924344 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.6290810516695635E-4 22-23 6.516324206678254E-4 24-25 0.0 26-27 0.002117805367170433 28-29 0.0041541566817573875 30-31 2.4436215775043454E-4 32-33 2.0363513145869546E-4 34-35 0.0 36-37 4.072702629173909E-5 38-39 4.072702629173909E-5 40-41 0.0 42-43 0.009041399836766079 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 8.145405258347818E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.850891840421737E-4 84-85 4.072702629173909E-5 86-87 0.0 88-89 0.0 90-91 0.00940794307339173 92-93 0.0014254459202108682 94-95 0.0 96-97 0.0 98-99 2.850891840421737E-4 100-101 0.01209592680864651 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1227686.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.58284333254019 #Duplication Level Percentage of deduplicated Percentage of total 1 80.58143078604503 49.62433627608942 2 11.382807519053909 14.019713042607146 3 3.474163748799382 6.418466455617086 4 1.514602772264087 3.730941809414813 5 0.7898843896715576 2.432166330998133 6 0.47992266237083775 1.773300127711133 7 0.313579666479408 1.3517789231150112 8 0.2313022780842226 1.139540156297626 9 0.15874476238362586 0.8798358448558937 >10 0.9740146844862241 11.093534778888776 >50 0.06666895792567962 2.7897669406121968 >100 0.031817204010526796 3.2636101129734163 >500 5.302842127727624E-4 0.19765291908526414 >1k 3.977131595795719E-4 0.8141242912508277 >5k 1.325710531931906E-4 0.4712319904833311 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5772 0.4701527915118361 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4964 0.4043379170243857 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3649 0.2972258378771119 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1359 0.11069605746094685 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.0727026291739095E-5 0.0 14-15 0.0 0.0 0.0 8.145405258347819E-5 0.0 16-17 0.0 0.0 0.0 2.0363513145869546E-4 0.0 18-19 0.0 0.0 0.0 2.4436215775043454E-4 8.145405258347819E-5 20-21 0.0 0.0 0.0 3.2581621033391276E-4 8.145405258347819E-5 22-23 0.0 0.0 0.0 3.6654323662565184E-4 8.145405258347819E-5 24-25 0.0 0.0 0.0 5.294513417926081E-4 1.6290810516695638E-4 26-27 0.0 0.0 0.0 0.0010181756572934773 1.6290810516695638E-4 28-29 0.0 0.0 0.0 0.0020770783408786938 1.6290810516695638E-4 30-31 0.0 0.0 0.0 0.0044799728920913 1.6290810516695638E-4 32-33 0.0 0.0 0.0 0.008959945784182601 2.4436215775043454E-4 34-35 0.0 0.0 0.0 0.014132278123233463 2.4436215775043454E-4 36-37 0.0 0.0 0.0 0.020444967198453026 2.4436215775043454E-4 38-39 0.0 0.0 0.0 0.03091181295542997 2.4436215775043454E-4 40-41 0.0 0.0 0.0 0.04891315857637865 2.4436215775043454E-4 42-43 0.0 0.0 0.0 0.06740322851282819 2.4436215775043454E-4 44-45 0.0 0.0 0.0 0.09114708484091208 2.4436215775043454E-4 46-47 0.0 0.0 0.0 0.1286566760556038 3.2581621033391276E-4 48-49 0.0 0.0 0.0 0.1634782835350407 3.2581621033391276E-4 50-51 0.0 0.0 0.0 0.2010693288023159 3.2581621033391276E-4 52-53 0.0 0.0 0.0 0.23589093628175284 3.2581621033391276E-4 54-55 4.0727026291739095E-5 0.0 0.0 0.27612923825799107 3.2581621033391276E-4 56-57 8.145405258347819E-5 0.0 0.0 0.3380343182214345 3.2581621033391276E-4 58-59 8.145405258347819E-5 0.0 0.0 0.3976586847125405 3.2581621033391276E-4 60-61 8.145405258347819E-5 0.0 0.0 0.4539027080214322 3.2581621033391276E-4 62-63 8.145405258347819E-5 0.0 0.0 0.5175997771417122 3.2581621033391276E-4 64-65 8.145405258347819E-5 0.0 0.0 0.5891978893625894 3.2581621033391276E-4 66-67 8.145405258347819E-5 0.0 0.0 0.6759464553639938 3.2581621033391276E-4 68-69 8.145405258347819E-5 0.0 0.0 0.779067285934677 3.2581621033391276E-4 70-71 8.145405258347819E-5 0.0 0.0 0.8968905729966783 3.2581621033391276E-4 72-73 8.145405258347819E-5 0.0 0.0 1.0245290733949886 4.072702629173909E-4 74-75 8.145405258347819E-5 0.0 0.0 1.1743638031222967 4.072702629173909E-4 76-77 8.145405258347819E-5 0.0 0.0 1.3463540351523111 4.072702629173909E-4 78-79 8.145405258347819E-5 0.0 0.0 1.5360197965929399 4.072702629173909E-4 80-81 8.145405258347819E-5 0.0 0.0 1.745804709021688 4.072702629173909E-4 82-83 8.145405258347819E-5 0.0 0.0 1.9643866591294517 4.072702629173909E-4 84-85 8.145405258347819E-5 0.0 0.0 2.203535757514544 4.072702629173909E-4 86-87 8.145405258347819E-5 0.0 0.0 2.4438252126358044 4.072702629173909E-4 88-89 8.145405258347819E-5 0.0 0.0 2.6898164514379084 4.072702629173909E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2875 0.0 58.185238 1 GTATCAA 5215 0.0 41.0989 1 GTGGTAT 1205 0.0 33.128384 1 TGGTATC 1245 0.0 30.52099 2 TATCAAC 7095 0.0 29.991873 2 ATCAACG 7245 0.0 29.174242 3 TCAACGC 7270 0.0 29.008583 4 CAACGCA 7410 0.0 28.460512 5 AACGCAG 7755 0.0 27.19438 6 TATACCG 105 6.907906E-5 27.14188 5 GGACCGT 95 0.00122686 24.9991 6 GTGTAGG 615 0.0 23.182173 1 ACGCAGA 9100 0.0 22.915876 7 CGCAGAG 9095 0.0 22.771786 8 TGCACCG 275 5.2750693E-11 22.453737 5 GCACCGT 280 6.548362E-11 22.052776 6 AGTACTT 6290 0.0 21.560995 12-13 GCAGAGT 9525 0.0 21.145319 9 GAGTACT 6130 0.0 20.922647 12-13 AGGCCCG 395 0.0 20.443968 8 >>END_MODULE