FastQCFastQC Report
Thu 26 May 2016
SRR937442_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937442_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225396
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66820.5452931134098692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46400.37865310479224673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31990.2610584659979305No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15920.1299171859545812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23300.059.9924851
GTATCAA46850.047.2913551
ATCAACG60000.036.830843
TCAACGC60250.036.599144
TATCAAC61100.036.40262
CAACGCA61400.035.9165885
AACGCAG63650.034.7031676
GTGGTAT8300.033.7970961
ACGCAGA72900.029.8449087
TGGTATC9000.029.5714912
CGCAGAG73200.029.5279058
GCAGAGT77500.027.3992189
GTACATG44450.026.3129041
TACATGG44100.025.7565142
ACATGGG46300.024.1210843
GAGTACT52100.023.61438212-13
CAGAGTA75050.022.69143110-11
GTACTTT55300.021.77412614-15
AGAGTAC74600.021.58660110-11
AGTACTT53700.021.27430312-13
Produced by FastQC (version 0.11.5)