FastQCFastQC Report
Thu 26 May 2016
SRR937442_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937442_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225396
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59960.4893112104168775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52110.4252502864380167No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37240.3039017591048118No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14320.11686018234105545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27850.062.6118431
GTATCAA52100.043.7742921
ATCAACG72000.031.6044143
TATCAAC72550.031.3648222
TCAACGC73550.030.938384
CAACGCA74650.030.5461275
AACGCAG77550.029.4602976
TATCACG856.4030156E-427.9444642
GTGGTAT11300.027.3306881
TGGTATC11150.025.5635472
ACGCAGA91050.024.7802267
CGCAGAG92300.024.2902438
GCAGAGT97950.022.646659
AGTACTT63150.022.11390912-13
GAGTACT61900.021.56290212-13
AGAGTAC90300.021.33018710-11
GTACTTT65050.020.33535414-15
CAGAGTA94350.019.93631410-11
GCACCGT2552.676461E-718.6266026
GAGTACA42900.018.6066251
Produced by FastQC (version 0.11.5)