##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937442_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1225396 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.07635898925735 31.0 31.0 33.0 30.0 34.0 2 31.35366771231504 31.0 31.0 34.0 30.0 34.0 3 31.35786309078861 31.0 31.0 34.0 28.0 34.0 4 35.178786286229105 35.0 35.0 37.0 32.0 37.0 5 34.89455490306807 35.0 35.0 37.0 32.0 37.0 6 35.10109466654045 36.0 35.0 37.0 32.0 37.0 7 35.064068268543394 36.0 35.0 37.0 32.0 37.0 8 35.04980022784471 36.0 35.0 37.0 32.0 37.0 9 36.523307567512866 38.0 35.0 39.0 32.0 39.0 10-11 36.386883097382395 38.0 35.0 39.0 32.0 39.0 12-13 36.2465962023705 38.0 35.0 39.0 32.0 39.0 14-15 37.09281897443765 38.5 36.0 40.0 32.0 41.0 16-17 37.02806194895364 38.0 36.0 40.0 31.0 41.0 18-19 37.00963443654133 38.5 36.0 40.0 31.0 41.0 20-21 36.875151379635646 39.0 36.0 40.0 31.0 41.0 22-23 36.672900025787584 38.0 35.5 40.0 30.5 41.0 24-25 36.45211139909058 38.0 35.0 40.0 30.0 41.0 26-27 36.09011413453284 38.0 34.5 40.0 29.5 41.0 28-29 35.99218701546276 38.0 34.0 40.0 29.0 41.0 30-31 36.111395418297434 38.0 35.0 40.0 30.0 41.0 32-33 36.02388452386005 38.0 35.0 40.0 30.0 41.0 34-35 35.834412712298715 38.0 34.5 40.0 29.5 41.0 36-37 35.56803310929691 38.0 34.0 40.0 28.5 41.0 38-39 35.34503580883241 38.0 34.0 40.0 27.0 41.0 40-41 35.05813998087149 38.0 34.0 40.0 27.0 41.0 42-43 34.739266734998324 38.0 33.0 40.0 26.0 41.0 44-45 34.29713986335846 37.5 33.0 40.0 24.5 41.0 46-47 33.80824688508858 37.0 33.0 40.0 23.5 41.0 48-49 33.35757298048957 37.0 32.0 40.0 22.5 40.5 50-51 33.02018857577469 37.0 31.0 39.0 20.0 40.0 52-53 32.63875963362048 36.5 31.0 39.0 18.5 40.0 54-55 32.31691143108024 36.0 31.0 39.0 17.5 40.0 56-57 31.9572460657616 36.0 30.0 39.0 14.5 40.0 58-59 31.59578413835201 35.0 30.0 39.0 13.0 40.0 60-61 31.103249480168042 35.0 29.0 38.0 8.5 40.0 62-63 30.54964599198953 34.0 28.0 38.0 8.0 40.0 64-65 30.06204198479512 34.0 28.0 38.0 7.0 40.0 66-67 29.517191177382657 34.0 27.5 37.0 6.0 39.0 68-69 28.909552911875018 33.0 26.0 36.5 2.0 39.0 70-71 28.44659155081296 33.0 26.0 36.0 2.0 39.0 72-73 27.88490128905268 32.0 25.5 36.0 2.0 38.5 74-75 27.308470486275457 32.0 25.0 35.0 2.0 38.0 76-77 26.46642432323918 30.5 24.0 34.0 2.0 36.0 78-79 27.072945398875138 32.0 25.0 35.0 2.0 37.0 80-81 26.79109528674812 32.0 25.0 35.0 2.0 36.0 82-83 26.3091270087384 31.5 24.0 34.5 2.0 36.0 84-85 25.764053824233144 31.0 22.5 34.0 2.0 35.5 86-87 25.172026430639562 31.0 20.0 34.0 2.0 35.0 88-89 24.5111380321137 31.0 18.5 34.0 2.0 35.0 90-91 23.6758039849975 30.0 11.5 34.0 2.0 35.0 92-93 22.841507153605853 29.0 4.5 34.0 2.0 35.0 94-95 21.96586817649152 29.0 2.0 34.0 2.0 35.0 96-97 21.0114979157758 29.0 2.0 33.5 2.0 34.5 98-99 19.778850673578173 27.0 2.0 33.0 2.0 34.0 100-101 17.945199755834032 22.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0954397584125104 1101 2 0.16838443781398382 1101 3 0.24218240952415826 1101 4 0.23963258885361682 1101 5 -0.10301712255974849 1101 6 0.13271350303871543 1101 7 0.1665184760885623 1101 8 0.0267151484786865 1101 9 0.18592784500955872 1101 10-11 0.06259931376116867 1101 12-13 0.1055379479255123 1101 14-15 0.25682027528286255 1101 16-17 0.09376409354344872 1101 18-19 0.24575851917378344 1101 20-21 0.019679960797851948 1101 22-23 0.05594410855591292 1101 24-25 0.20371943139293336 1101 26-27 0.033552314829456975 1101 28-29 0.08254772139301281 1101 30-31 0.18766744538535818 1101 32-33 0.26352259022528557 1101 34-35 0.15505787424581996 1101 36-37 0.1654133674833247 1101 38-39 0.30724119718610154 1101 40-41 0.29924123779797895 1101 42-43 0.30672643975078273 1101 44-45 0.31377798956687997 1101 46-47 0.11893198158200136 1101 48-49 -0.09279324784133536 1101 50-51 0.15556775712216364 1101 52-53 0.32888025351024197 1101 54-55 0.1673160466446646 1101 56-57 0.012447800223451821 1101 58-59 0.08058672063874539 1101 60-61 0.03433258404117012 1101 62-63 -0.052959429695750515 1101 64-65 0.32546548353542093 1101 66-67 0.30529748430733505 1101 68-69 0.18565290106467458 1101 70-71 -0.03390210616366929 1101 72-73 -0.028986147510632776 1101 74-75 0.06381163290123126 1101 76-77 0.31976685692938034 1101 78-79 0.20626014379072544 1101 80-81 -0.004564352759125256 1101 82-83 0.04507600956562996 1101 84-85 -0.07943190299160463 1101 86-87 -0.3531050319481501 1101 88-89 -0.222047876734365 1101 90-91 -0.18287277447366534 1101 92-93 -0.1716615270238364 1101 94-95 -0.5007756082375465 1101 96-97 -0.1757752778121322 1101 98-99 -0.349109683898984 1101 100-101 -0.16396941001640997 1102 1 0.033125562133829334 1102 2 0.1187148873902899 1102 3 0.057860746121807693 1102 4 0.08899823308976806 1102 5 0.010930522635710815 1102 6 0.10357323403240315 1102 7 0.20564280657061573 1102 8 0.16448532894875 1102 9 0.19949682534372215 1102 10-11 0.18508346891945138 1102 12-13 0.138779170495269 1102 14-15 0.15573165284101975 1102 16-17 0.025936210868970022 1102 18-19 0.10112078334358188 1102 20-21 0.1682668844628168 1102 22-23 0.24804407782804105 1102 24-25 0.2976986413617553 1102 26-27 0.20748442745836115 1102 28-29 0.2046343502702186 1102 30-31 0.18519469451597814 1102 32-33 -0.04654556695154355 1102 34-35 0.09153064510079645 1102 36-37 0.05918485201446799 1102 38-39 -0.03362946968375269 1102 40-41 -0.05853350813494984 1102 42-43 0.05269699719941201 1102 44-45 0.03277827810912726 1102 46-47 -0.016815748802123665 1102 48-49 -0.2242239302499982 1102 50-51 -0.16468915789901928 1102 52-53 -0.07866459544850812 1102 54-55 -0.09310004751753098 1102 56-57 -0.05492213092612275 1102 58-59 -0.017524201996955924 1102 60-61 -0.14728321508346198 1102 62-63 -0.25423029592206703 1102 64-65 -0.04239684308940994 1102 66-67 -0.11415785160081171 1102 68-69 -0.14426939862954669 1102 70-71 -0.3131841480934803 1102 72-73 -0.15239900900036218 1102 74-75 -0.4713030845022388 1102 76-77 -0.3160172535111023 1102 78-79 -0.18582802148027966 1102 80-81 -1.2879635973561676E-4 1102 82-83 -0.11901100403457932 1102 84-85 -0.028323544759533803 1102 86-87 -0.11275558355099591 1102 88-89 -0.13201035999641775 1102 90-91 -0.27826893298953337 1102 92-93 -0.26829070401640465 1102 94-95 0.04815197640151325 1102 96-97 0.2956187559835328 1102 98-99 0.26102976569730885 1102 100-101 0.23903277216242103 1103 1 0.08941006560390363 1103 2 0.1517653379515025 1103 3 0.162119767961304 1103 4 0.15324828109596922 1103 5 0.07300048684810889 1103 6 0.15412741260027474 1103 7 0.1703457289602639 1103 8 -0.015744860015082907 1103 9 0.055626821712358776 1103 10-11 0.21365920243209757 1103 12-13 0.34919068666116004 1103 14-15 0.23023142184086254 1103 16-17 0.10056827419786885 1103 18-19 0.20604634679067146 1103 20-21 0.29512114395996747 1103 22-23 0.2561534825447538 1103 24-25 0.1803855756252588 1103 26-27 0.1369763031606297 1103 28-29 0.1552418268168978 1103 30-31 0.1393221508335003 1103 32-33 0.29450937179380077 1103 34-35 0.2908333985339553 1103 36-37 0.2964904585689112 1103 38-39 0.45525558856694914 1103 40-41 0.408535660727118 1103 42-43 0.34305532312321674 1103 44-45 0.23265583851931382 1103 46-47 0.2632568910828823 1103 48-49 0.16275235056507142 1103 50-51 0.04885464435724174 1103 52-53 0.08931640613521807 1103 54-55 0.09653999149582404 1103 56-57 0.019736391268587283 1103 58-59 0.030424808124589475 1103 60-61 -0.06348545207757894 1103 62-63 0.08096503388215126 1103 64-65 0.15920054359851932 1103 66-67 0.3318281958629079 1103 68-69 0.17185336670534568 1103 70-71 0.16366275995831714 1103 72-73 -0.09410600827345306 1103 74-75 -0.07010682689491787 1103 76-77 0.03179388532284122 1103 78-79 0.33924991334662025 1103 80-81 0.15558115363142733 1103 82-83 0.11083922294129778 1103 84-85 0.1520059837783343 1103 86-87 0.5484936171377583 1103 88-89 0.39186187441836395 1103 90-91 0.41709366754623645 1103 92-93 0.2477512904814212 1103 94-95 0.08843995659823989 1103 96-97 0.39680791564361684 1103 98-99 0.4852390258567212 1103 100-101 0.5370823893485799 1104 1 -0.028344123915321262 1104 2 0.20007686453567075 1104 3 0.11715128402614994 1104 4 0.07384707021388692 1104 5 0.00760956711013705 1104 6 0.1318508820939286 1104 7 0.0381213503059783 1104 8 0.03318480556463044 1104 9 0.08731337432088537 1104 10-11 0.22745662558529745 1104 12-13 0.2084488489642169 1104 14-15 0.16631510845019193 1104 16-17 0.12537537164532608 1104 18-19 0.1639295849108322 1104 20-21 0.20105379581492855 1104 22-23 0.10459437356730206 1104 24-25 -0.05861071231793602 1104 26-27 0.028268761542598497 1104 28-29 -0.0030062680065370273 1104 30-31 0.021229402912389617 1104 32-33 0.09440330842391376 1104 34-35 0.10174990078252932 1104 36-37 -0.10255049136698347 1104 38-39 -0.119025832907802 1104 40-41 0.17692803525101652 1104 42-43 0.21813549104560082 1104 44-45 0.24034293514171878 1104 46-47 0.297891367253591 1104 48-49 0.05457931547682193 1104 50-51 0.2084455317379863 1104 52-53 0.1191599828073322 1104 54-55 -0.024798308435016736 1104 56-57 0.1255778692331262 1104 58-59 0.053683174110570775 1104 60-61 -0.08651660847448284 1104 62-63 0.11795846124050513 1104 64-65 0.06701935732597164 1104 66-67 -0.03560564424986268 1104 68-69 -0.2158826976120487 1104 70-71 0.04148829651134278 1104 72-73 -0.0786568842067048 1104 74-75 -0.38994477005836004 1104 76-77 -0.1734166590068753 1104 78-79 -0.4224426788472968 1104 80-81 -0.12437973409094027 1104 82-83 -0.12086847143892498 1104 84-85 -0.059048036671171644 1104 86-87 6.406994121235243E-4 1104 88-89 0.2203421613276717 1104 90-91 0.34142401159634517 1104 92-93 0.4574025737160632 1104 94-95 0.16714423026558478 1104 96-97 0.25232259853960315 1104 98-99 0.21021551530420268 1104 100-101 0.3403870480797764 1105 1 0.08725318339800126 1105 2 0.11494223515666135 1105 3 0.17840828735352687 1105 4 0.05917200049029958 1105 5 -0.061799885701283586 1105 6 -0.004962943773321626 1105 7 0.06705792140818545 1105 8 0.1118546217810632 1105 9 0.02621757015673154 1105 10-11 0.18966417545381375 1105 12-13 -0.0756294964717199 1105 14-15 0.14985214841734518 1105 16-17 0.08536230394266653 1105 18-19 0.0770102033210378 1105 20-21 0.1563849461328175 1105 22-23 0.09877666934067975 1105 24-25 0.1872270955584341 1105 26-27 0.2966807284663773 1105 28-29 0.2260935833136699 1105 30-31 -0.007097220946207017 1105 32-33 0.024133851470203638 1105 34-35 0.0803512073546031 1105 36-37 0.059923565642684196 1105 38-39 0.08961196479450706 1105 40-41 0.02266555610699328 1105 42-43 -0.3875384882713604 1105 44-45 -0.027209523128995272 1105 46-47 -0.036274332933800224 1105 48-49 0.029226783146974356 1105 50-51 0.14063584849103705 1105 52-53 0.2944632469912918 1105 54-55 0.3586003579061696 1105 56-57 0.14054825053851516 1105 58-59 0.03701491637292875 1105 60-61 0.008415824962320073 1105 62-63 -0.11811341905193729 1105 64-65 0.32755155900594346 1105 66-67 0.16825728889443425 1105 68-69 0.07713179610861687 1105 70-71 0.07006613341016177 1105 72-73 0.2526533115412697 1105 74-75 0.21340536476425953 1105 76-77 0.17889765557791648 1105 78-79 0.038570411647867786 1105 80-81 0.36994724453794703 1105 82-83 0.3402679958748074 1105 84-85 -0.006557393985303861 1105 86-87 0.0693335096321519 1105 88-89 0.12727260223711312 1105 90-91 0.023438016764625758 1105 92-93 0.1060323312797884 1105 94-95 0.10496066207586097 1105 96-97 0.11329013705810809 1105 98-99 0.3627910187790704 1105 100-101 0.538628160406855 1106 1 -0.36851472309874467 1106 2 -0.10930023432657521 1106 3 -0.1410621644308243 1106 4 0.019759698982234397 1106 5 -0.1301563192754145 1106 6 -0.14644502934529413 1106 7 0.024640318072940204 1106 8 -0.1383923675984846 1106 9 -0.19031382931289897 1106 10-11 -0.15021523177167495 1106 12-13 -0.4247142267988764 1106 14-15 -0.07005461705751515 1106 16-17 -0.024036169146036457 1106 18-19 -0.1224510295170802 1106 20-21 -0.2547399332650784 1106 22-23 -0.21569744949415082 1106 24-25 -0.09506114394009302 1106 26-27 0.029379248396288915 1106 28-29 -0.18304623674892184 1106 30-31 0.006155294202535799 1106 32-33 -0.29046530165574325 1106 34-35 -0.07728792128702366 1106 36-37 -0.22542838722498715 1106 38-39 -0.3845535246214311 1106 40-41 -0.147177105966378 1106 42-43 -0.39961381490011405 1106 44-45 -0.21769683961318975 1106 46-47 -0.1252793399039973 1106 48-49 -0.2776667118973748 1106 50-51 -0.13927319372762526 1106 52-53 -0.11008912091558187 1106 54-55 -0.2223513210057675 1106 56-57 -0.18799539855927705 1106 58-59 -0.22370136215868186 1106 60-61 -0.20023225576131054 1106 62-63 -0.25476470214637104 1106 64-65 -0.4105068264094598 1106 66-67 -0.028632343150174933 1106 68-69 0.08862879248635736 1106 70-71 -0.03200514360051088 1106 72-73 -0.058528486451837836 1106 74-75 -0.07043274008912093 1106 76-77 -0.10340364720475392 1106 78-79 -0.13184759478300023 1106 80-81 -0.09561999125246601 1106 82-83 -0.2623153618744247 1106 84-85 -0.30954627339812646 1106 86-87 -0.0462808472903653 1106 88-89 0.1258748308333857 1106 90-91 0.19145704437107725 1106 92-93 0.11280247159811907 1106 94-95 0.1163641626627765 1106 96-97 0.39751472850389646 1106 98-99 0.7015204837629376 1106 100-101 0.6287741519381136 1107 1 -0.011382295590248503 1107 2 -0.08441113240685638 1107 3 0.0023888018637023833 1107 4 0.010236777086880977 1107 5 -0.12949281700419846 1107 6 -0.024949109107588185 1107 7 -0.0013343534490530828 1107 8 -0.18695052612449103 1107 9 -0.0895746567157687 1107 10-11 -0.0944661732126093 1107 12-13 0.13430374545829693 1107 14-15 0.12840601329182988 1107 16-17 -0.03183842327850073 1107 18-19 0.09898164719310643 1107 20-21 -0.11068338245610931 1107 22-23 -0.01569878093683741 1107 24-25 0.12797214878424512 1107 26-27 0.05751684855961514 1107 28-29 -0.0654301404944917 1107 30-31 -0.08585227726819511 1107 32-33 -0.12032645437444245 1107 34-35 0.032981496140230604 1107 36-37 -0.0899782819300583 1107 38-39 -0.18035126960133852 1107 40-41 -0.14738547674654257 1107 42-43 -0.09279882551837204 1107 44-45 -0.03270153294864997 1107 46-47 -0.11962559045043974 1107 48-49 -0.2391360920442338 1107 50-51 -0.14030439608693968 1107 52-53 -0.3182484235946603 1107 54-55 -0.47984767618142143 1107 56-57 -0.20084315474549186 1107 58-59 -0.31895876331570605 1107 60-61 -0.11215044304647392 1107 62-63 -0.0063984932740801526 1107 64-65 0.02182126769691095 1107 66-67 0.013734606891876666 1107 68-69 0.07186348810746068 1107 70-71 -0.07212350885518148 1107 72-73 0.05585819557080285 1107 74-75 0.18648244035464856 1107 76-77 0.1376207647968748 1107 78-79 0.08756972151674347 1107 80-81 0.11037482943548937 1107 82-83 0.08239026724333698 1107 84-85 -0.05825754783287351 1107 86-87 -0.04433605334221369 1107 88-89 0.40323653277219407 1107 90-91 0.3251028229210924 1107 92-93 0.39335098160280424 1107 94-95 0.18324408206611054 1107 96-97 0.14875618785384503 1107 98-99 0.373317421000948 1107 100-101 0.48814384697116964 1108 1 -0.13211514023613802 1108 2 -0.11688040291960533 1108 3 -0.28666049588124665 1108 4 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94-95 -1.2563788983549635 2316 96-97 -0.6516115669510789 2316 98-99 -1.0407376193197244 2316 100-101 -0.8646596925506564 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 17.0 4 28.0 5 84.0 6 174.0 7 471.0 8 1006.0 9 1737.0 10 2512.0 11 3699.0 12 5452.0 13 7677.0 14 9641.0 15 12897.0 16 16279.0 17 20342.0 18 17861.0 19 13850.0 20 13641.0 21 14870.0 22 15856.0 23 17752.0 24 20892.0 25 25000.0 26 29441.0 27 35818.0 28 42555.0 29 50224.0 30 59301.0 31 69233.0 32 81941.0 33 97858.0 34 114808.0 35 132454.0 36 141183.0 37 111673.0 38 35961.0 39 1203.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.48623911627322 23.787953247117947 16.334937150712577 19.390870485896258 2 18.94979255685509 24.487839033259455 35.90724957483132 20.655118835054136 3 21.768228393107208 26.323898560138925 30.769481865454107 21.13839118129976 4 16.40424809612566 20.29499035413858 35.450254448357924 27.850507101377843 5 13.620162953568107 38.96488655033189 33.966996311794176 13.447954184305825 6 31.039353808891168 39.0224874244734 16.26070266264946 13.677456103985977 7 27.370662218580772 34.51773957153443 20.66213697449641 17.449461235388398 8 26.450714707735294 36.476371719835875 19.12304267355206 17.94987089887677 9 26.230132952939293 16.8072198701481 20.315799953647637 36.64684722326497 10-11 25.006528501806763 28.137597968330237 26.984705352392208 19.871168177470793 12-13 25.682608131477302 25.351311516750087 28.02482092681442 20.941259424958194 14-15 22.63243881977744 26.69940982343667 26.493476394569594 24.174674962216297 16-17 21.561234082696533 30.084805238469848 27.123354409513333 21.230606269320283 18-19 21.477995684660307 28.017310322540634 30.264624660109874 20.24006933268919 20-21 22.14341323131461 27.0918543882957 30.70758350769874 20.05714887269095 22-23 21.9803105734269 27.094281481191324 30.56606591351162 20.359342031870153 24-25 21.912216483003977 27.34195943412278 30.556814792357855 20.189009290515383 26-27 21.73296549990676 27.486190945046552 30.399547566517477 20.38129598852921 28-29 21.541485444931237 27.489761201472053 30.589494464295697 20.37925888930101 30-31 21.608366013498596 27.37699493180315 30.57508794164127 20.439551113056982 32-33 21.576004818034335 27.492745202367235 30.42355287596826 20.507697103630175 34-35 21.956004426324224 27.32732928783838 30.30428530858596 20.412380977251434 36-37 21.937778329074835 27.228693162112073 30.252335373223122 20.58119313558997 38-39 21.765515946931288 27.541583615045788 30.193264607054477 20.499635830968444 40-41 21.90320516796203 27.361032678415793 30.267358470241458 20.46840368338072 42-43 21.913797887795244 27.496082464130062 29.92911708535331 20.661002562721382 44-45 22.039324430633037 27.67635931568244 29.501320389490417 20.782995864194106 46-47 22.174301205487858 27.975283092159597 28.981366023718046 20.8690496786345 48-49 22.321355708685193 28.172566256132715 28.68762424555001 20.818453789632084 50-51 22.23171121825108 28.285427731117124 28.452026936598457 21.030834114033343 52-53 22.59208451798439 28.481160375911134 28.111279945421725 20.81547516068275 54-55 22.36901377187456 28.407755533721346 28.28657021893331 20.936660475470788 56-57 22.28695866479081 28.702517390296688 28.133109623338086 20.877414321574413 58-59 22.468246999337357 28.29701582182413 28.308318290577088 20.926418888261427 60-61 22.414550071976734 28.376337118776295 28.443580687385957 20.765532121861014 62-63 22.26712834055277 28.324517135685117 28.554524414964632 20.85383010879748 64-65 22.219755899317445 28.251724340539713 28.42297510355836 21.105544656584485 66-67 22.18544046169565 28.678443539884253 28.143881651319248 20.992234347100855 68-69 22.2363627757884 28.381641526494295 28.204474308713266 21.177521389004045 70-71 22.23607715383435 28.50396932909851 28.158529977248172 21.101423539818963 72-73 22.468532621291402 28.52175949652194 27.92150455852639 21.08820332366027 74-75 22.424342824686878 28.747278430809303 27.570883208366926 21.25749553613689 76-77 22.29960763704141 28.531837871186134 27.980954728104223 21.187599763668235 78-79 22.341349245468404 28.483730973497543 27.878294037192873 21.296625743841176 80-81 22.19209137291129 28.68456401032809 27.82080241815707 21.302542198603554 82-83 22.275996551297812 28.616720329919858 27.834274599443358 21.27300851933897 84-85 22.185408710901907 28.560248507522672 27.751285197309766 21.50305758426565 86-87 22.295078488912974 28.45773937567937 27.843203339981525 21.40397879542613 88-89 22.333596649572872 28.365891515885476 27.731402746540713 21.56910908800094 90-91 22.190828409006976 28.513349239713392 27.838164620831975 21.45765773044766 92-93 22.243503871705116 28.353604172472764 27.702369376622222 21.700522579199905 94-95 22.177851078345288 28.249153742953297 27.655100881674173 21.917894297027246 96-97 22.333474240163994 28.277838347766764 27.55798941729857 21.83069799477067 98-99 22.190508639174457 28.28507949591044 27.359079140917796 22.1653327239973 100-101 22.287424494816147 28.121309417611307 27.638386382500833 21.952879705071712 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 407.0 1 270.0 2 165.5 3 216.0 4 215.0 5 215.5 6 357.5 7 754.0 8 1159.0 9 1406.0 10 1450.0 11 1416.0 12 1562.5 13 1738.0 14 1927.0 15 2138.5 16 2311.5 17 2459.0 18 2674.5 19 2899.0 20 3073.5 21 3436.5 22 3994.5 23 4698.0 24 5576.5 25 6838.5 26 8660.0 27 10575.0 28 12442.0 29 14826.0 30 17473.5 31 20081.5 32 22943.0 33 26021.0 34 29190.5 35 31717.5 36 34507.0 37 37336.0 38 38835.0 39 39474.5 40 40029.5 41 41240.5 42 42916.5 43 44478.5 44 45778.5 45 46710.5 46 46036.5 47 44484.0 48 44533.0 49 44321.5 50 42690.0 51 40513.0 52 37821.0 53 35771.0 54 34070.5 55 31275.0 56 27672.5 57 24108.0 58 21430.0 59 19212.5 60 16261.0 61 13154.5 62 10588.5 63 8468.5 64 6503.5 65 4899.5 66 3653.0 67 2754.5 68 1984.0 69 1319.0 70 923.5 71 657.0 72 515.0 73 414.5 74 302.0 75 224.0 76 171.0 77 115.0 78 66.0 79 42.5 80 20.5 81 11.0 82 6.5 83 4.5 84 1.5 85 1.5 86 2.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03280572157898345 2 0.0 3 0.0 4 0.0 5 0.011343271889250495 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00481477008248762 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001428109770229379 24-25 5.304407717994837E-4 26-27 0.0018361411331520585 28-29 0.002652203858997418 30-31 7.752595895530914E-4 32-33 0.0 34-35 0.0 36-37 2.4481881775360783E-4 38-39 2.856219540458758E-4 40-41 0.0 42-43 0.011098453071496888 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.120470443840196E-4 84-85 4.0803136292267974E-5 86-87 0.0 88-89 0.0 90-91 0.008283036667330398 92-93 0.0025705975864128818 94-95 0.0 96-97 0.0 98-99 0.0015097160428139149 100-101 0.012159334615095855 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1225396.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.400879684179884 #Duplication Level Percentage of deduplicated Percentage of total 1 80.51527146916663 49.43708496217382 2 11.442871308026263 14.052047288513494 3 3.462542476576485 6.37809462016905 4 1.48624583112827 3.6502720583288335 5 0.8105527351291023 2.488432548367247 6 0.4937775188897138 1.8191024416860107 7 0.3243589602503121 1.394114784497057 8 0.22741071208297672 1.1170574217200424 9 0.17263262716641076 0.9539815653187804 >10 0.9624029682009934 10.938162737903557 >50 0.06906102528174048 2.8856799817645866 >100 0.031541508316060396 3.254755565207099 >500 7.985158710894837E-4 0.29409574559849644 >1k 2.661719570298279E-4 0.4213274977232141 >5k 2.661719570298279E-4 0.9157907810287161 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5996 0.4893112104168775 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5211 0.4252502864380167 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3724 0.3039017591048118 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1432 0.11686018234105545 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 8.160627258453593E-5 7 0.0 0.0 0.0 0.0 8.160627258453593E-5 8 0.0 0.0 0.0 0.0 8.160627258453593E-5 9 0.0 0.0 0.0 0.0 8.160627258453593E-5 10-11 0.0 0.0 0.0 4.080313629226797E-5 8.160627258453593E-5 12-13 0.0 0.0 0.0 8.160627258453593E-5 8.160627258453593E-5 14-15 0.0 0.0 0.0 8.160627258453593E-5 8.160627258453593E-5 16-17 0.0 0.0 0.0 1.6321254516907187E-4 8.160627258453593E-5 18-19 0.0 0.0 0.0 1.6321254516907187E-4 8.160627258453593E-5 20-21 0.0 0.0 0.0 2.0401568146133985E-4 8.160627258453593E-5 22-23 0.0 0.0 0.0 3.2642509033814374E-4 1.2240940887680391E-4 24-25 0.0 0.0 0.0 5.712439080917516E-4 1.6321254516907187E-4 26-27 0.0 0.0 0.0 0.001468912906521647 1.6321254516907187E-4 28-29 0.0 0.0 0.0 0.002774613267874222 1.6321254516907187E-4 30-31 0.0 0.0 0.0 0.005794045353502052 1.6321254516907187E-4 32-33 0.0 0.0 0.0 0.008895083711714418 1.6321254516907187E-4 34-35 0.0 0.0 0.0 0.01285298793206441 1.6321254516907187E-4 36-37 0.0 0.0 0.0 0.01803498624118244 1.6321254516907187E-4 38-39 0.0 0.0 0.0 0.02754211699728088 1.6321254516907187E-4 40-41 0.0 0.0 0.0 0.0430473087883427 1.6321254516907187E-4 42-43 0.0 0.0 0.0 0.05924615389637308 1.6321254516907187E-4 44-45 0.0 0.0 0.0 0.07895406872553852 1.6321254516907187E-4 46-47 0.0 0.0 0.0 0.11245344362149053 1.6321254516907187E-4 48-49 0.0 0.0 0.0 0.14321900838586057 1.6321254516907187E-4 50-51 0.0 0.0 0.0 0.17945219341339452 1.6321254516907187E-4 52-53 0.0 0.0 0.0 0.2142572686706991 1.6321254516907187E-4 54-55 0.0 0.0 0.0 0.2510616976063248 1.6321254516907187E-4 56-57 0.0 0.0 0.0 0.3082268915517923 2.0401568146133985E-4 58-59 0.0 0.0 0.0 0.3628214879108468 2.4481881775360783E-4 60-61 0.0 0.0 0.0 0.41378460513988946 2.4481881775360783E-4 62-63 0.0 0.0 0.0 0.4728675464910935 2.4481881775360783E-4 64-65 0.0 0.0 0.0 0.5397030837378285 2.4481881775360783E-4 66-67 0.0 0.0 0.0 0.6195956245980891 2.4481881775360783E-4 68-69 0.0 0.0 0.0 0.7115250906645689 2.4481881775360783E-4 70-71 0.0 0.0 0.0 0.8267531475539336 2.4481881775360783E-4 72-73 0.0 0.0 0.0 0.9504274536557977 2.4481881775360783E-4 74-75 0.0 0.0 0.0 1.0891581170495088 2.4481881775360783E-4 76-77 0.0 0.0 0.0 1.2512689775386896 2.4481881775360783E-4 78-79 0.0 0.0 0.0 1.4265592510502727 2.4481881775360783E-4 80-81 0.0 0.0 0.0 1.6297996729220594 2.4481881775360783E-4 82-83 0.0 0.0 0.0 1.8436081070935437 2.4481881775360783E-4 84-85 0.0 0.0 0.0 2.0745130553714883 2.4481881775360783E-4 86-87 0.0 0.0 0.0 2.306070853830109 2.4481881775360783E-4 88-89 0.0 0.0 0.0 2.5663948633747786 2.4481881775360783E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2785 0.0 62.611843 1 GTATCAA 5210 0.0 43.774292 1 ATCAACG 7200 0.0 31.604414 3 TATCAAC 7255 0.0 31.364822 2 TCAACGC 7355 0.0 30.93838 4 CAACGCA 7465 0.0 30.546127 5 AACGCAG 7755 0.0 29.460297 6 TATCACG 85 6.4030156E-4 27.944464 2 GTGGTAT 1130 0.0 27.330688 1 TGGTATC 1115 0.0 25.563547 2 ACGCAGA 9105 0.0 24.780226 7 CGCAGAG 9230 0.0 24.290243 8 GCAGAGT 9795 0.0 22.64665 9 AGTACTT 6315 0.0 22.113909 12-13 GAGTACT 6190 0.0 21.562902 12-13 AGAGTAC 9030 0.0 21.330187 10-11 GTACTTT 6505 0.0 20.335354 14-15 CAGAGTA 9435 0.0 19.936314 10-11 GCACCGT 255 2.676461E-7 18.626602 6 GAGTACA 4290 0.0 18.606625 1 >>END_MODULE