FastQCFastQC Report
Thu 26 May 2016
SRR937441_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937441_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267092
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66680.5262443453198348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47710.37653145943625244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33760.26643684910014426No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16660.1314821654623342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.058.8344881
GTATCAA48450.046.8070561
ATCAACG62850.036.14413
TATCAAC63150.035.973822
TCAACGC64350.035.304384
CAACGCA65950.034.5199285
GTGGTAT9300.034.251421
AACGCAG67800.033.5647286
TGGTATC9800.031.5224042
ACGCAGA76300.029.451947
CGCAGAG75900.029.1689978
GCAGAGT79450.027.5644919
GTACATG47650.027.438261
TATCACG700.00836944127.157762
TACATGG48850.025.7818052
AGTACGT950.001222702925.013732
ACATGGG48600.024.6421783
GAGTACT51850.022.67570712-13
CAGAGTA78100.022.56658710-11
AGAGTAC76400.022.16711410-11
Produced by FastQC (version 0.11.5)