##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937441_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1267092 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16916687975301 33.0 31.0 34.0 31.0 34.0 2 32.30415628857257 34.0 31.0 34.0 31.0 34.0 3 32.35789035050336 34.0 31.0 34.0 31.0 34.0 4 35.94677103162201 37.0 35.0 37.0 35.0 37.0 5 35.937343144775596 37.0 35.0 37.0 35.0 37.0 6 36.01150587329097 37.0 37.0 37.0 35.0 37.0 7 35.975439825995274 37.0 37.0 37.0 35.0 37.0 8 35.97104787971197 37.0 37.0 37.0 35.0 37.0 9 37.67662253411749 39.0 38.0 39.0 35.0 39.0 10-11 37.601831595495824 39.0 38.0 39.0 35.0 39.0 12-13 37.52209823753918 39.0 37.5 39.0 35.0 39.0 14-15 38.8090359658178 40.0 38.0 41.0 36.0 41.0 16-17 38.7541611027455 40.0 38.0 41.0 36.0 41.0 18-19 38.71815700833089 40.0 38.0 41.0 35.5 41.0 20-21 38.65517539373621 40.0 38.0 41.0 35.0 41.0 22-23 38.52456490925678 40.0 38.0 41.0 35.0 41.0 24-25 38.39448240538177 40.0 38.0 41.0 34.0 41.0 26-27 38.23493795241387 40.0 38.0 41.0 34.0 41.0 28-29 38.051844301755516 40.0 38.0 41.0 34.0 41.0 30-31 38.0382553121636 40.0 38.0 41.0 34.0 41.0 32-33 37.89330214380645 40.0 38.0 41.0 34.0 41.0 34-35 37.70809104626973 40.0 37.0 41.0 33.5 41.0 36-37 37.74367883310762 40.0 38.0 41.0 33.0 41.0 38-39 37.73343253686394 40.0 38.0 41.0 33.0 41.0 40-41 37.586308650042774 40.0 37.5 41.0 33.0 41.0 42-43 37.35200048615255 40.0 37.0 41.0 33.0 41.0 44-45 37.11720735353076 40.0 37.0 41.0 32.5 41.0 46-47 36.77398918152747 40.0 37.0 41.0 31.5 41.0 48-49 36.45746954443719 40.0 36.5 41.0 30.5 41.0 50-51 35.56237826456169 38.5 35.0 40.0 29.0 40.5 52-53 35.701604934764006 38.5 35.5 40.0 30.0 40.5 54-55 36.05698205023786 39.0 36.0 40.5 30.0 41.0 56-57 35.8627992284696 39.0 35.5 41.0 29.5 41.0 58-59 35.63393029077605 39.0 35.0 40.5 29.0 41.0 60-61 35.371830143351865 38.5 35.0 40.0 28.0 41.0 62-63 35.06891646384004 38.0 35.0 40.0 28.0 41.0 64-65 34.73265279869181 38.0 34.5 40.0 27.0 41.0 66-67 34.35940168511837 37.0 34.0 40.0 26.0 41.0 68-69 33.88841260145277 36.5 34.0 39.0 26.0 41.0 70-71 33.47623416452791 36.0 34.0 39.0 25.0 40.5 72-73 33.07082477041919 35.5 33.0 38.5 24.5 40.0 74-75 32.62257199950753 35.0 33.0 37.5 23.5 39.5 76-77 32.18720819009196 35.0 32.5 37.0 22.0 39.0 78-79 31.72770603870911 35.0 32.0 36.5 20.0 39.0 80-81 31.291869098692125 35.0 31.5 36.0 19.0 38.5 82-83 30.870371685718162 34.5 31.0 36.0 17.5 37.0 84-85 30.437723148753207 34.0 31.0 35.0 12.5 37.0 86-87 30.02373781856408 34.0 30.5 35.0 7.0 36.0 88-89 29.66291042797208 34.0 30.0 35.0 4.5 36.0 90-91 29.268002244509475 34.0 29.5 35.0 2.0 35.5 92-93 28.868705271598273 34.0 29.0 35.0 2.0 35.0 94-95 28.376974600107964 34.0 29.0 35.0 2.0 35.0 96-97 27.811269426371567 34.0 28.0 35.0 2.0 35.0 98-99 27.160684464900733 33.0 27.0 35.0 2.0 35.0 100-101 25.31676192415389 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.051367486461344924 1101 2 0.059297808425881726 1101 3 0.08557443407303822 1101 4 -0.03076994335038563 1101 5 0.08417834579210393 1101 6 -0.025796102488698125 1101 7 0.05227262372964958 1101 8 -0.028159679280534533 1101 9 -0.03675164759157923 1101 10-11 -0.11625923749506484 1101 12-13 0.04384433136338117 1101 14-15 0.008673178779979196 1101 16-17 -0.004008531670933735 1101 18-19 0.07898515259068262 1101 20-21 0.23437641803658948 1101 22-23 0.09826689589706206 1101 24-25 0.1322110608479008 1101 26-27 0.006900003455271531 1101 28-29 0.017331523099116453 1101 30-31 -0.011733903448316596 1101 32-33 0.04221044964561571 1101 34-35 0.0944249146675844 1101 36-37 0.09825074869089434 1101 38-39 0.1126421665613222 1101 40-41 0.12360478057169644 1101 42-43 0.16102688727207948 1101 44-45 0.13899313895865362 1101 46-47 0.26536537742942556 1101 48-49 0.38856095357903087 1101 50-51 0.3758674651277971 1101 52-53 0.3740182308985851 1101 54-55 0.43082960002571014 1101 56-57 0.4756372992265625 1101 58-59 0.43359177800795123 1101 60-61 0.5420077849388889 1101 62-63 0.5926361839887377 1101 64-65 0.3613243986746113 1101 66-67 0.4213227245686042 1101 68-69 0.39639363392159765 1101 70-71 0.5689038342635158 1101 72-73 0.4477949116068203 1101 74-75 0.5147965293962287 1101 76-77 0.534997222853363 1101 78-79 0.5006299495685944 1101 80-81 0.49683220296877195 1101 82-83 0.48733217905621373 1101 84-85 0.4281614688553894 1101 86-87 0.5814214842532941 1101 88-89 0.51568104695016 1101 90-91 0.42019046660005444 1101 92-93 0.5094549531589294 1101 94-95 0.2675142268440034 1101 96-97 0.2040879962128166 1101 98-99 0.459667801243576 1101 100-101 0.978240850147504 1102 1 0.09759750793249822 1102 2 0.055555306177105024 1102 3 0.10435901940781633 1102 4 0.01627996024658529 1102 5 0.06254950910570045 1102 6 0.09085785941464053 1102 7 0.0696881160712266 1102 8 0.12684147579332716 1102 9 0.07060538605286126 1102 10-11 0.058383838387932485 1102 12-13 0.1873257491520235 1102 14-15 0.24274732256180442 1102 16-17 0.16639539167748296 1102 18-19 0.22537109329605443 1102 20-21 0.25770072689641665 1102 22-23 0.3671441704011542 1102 24-25 0.39709876689639856 1102 26-27 0.2739938376269464 1102 28-29 0.33015621058396505 1102 30-31 0.2954501209045475 1102 32-33 0.1056090546185331 1102 34-35 0.2668236222437983 1102 36-37 0.17941105342896435 1102 38-39 0.3901304065246123 1102 40-41 0.27337222360326763 1102 42-43 0.2503022940185957 1102 44-45 0.19405979018236508 1102 46-47 0.26399596970220074 1102 48-49 0.3279008493265323 1102 50-51 0.28731972343882006 1102 52-53 0.29620575194642385 1102 54-55 0.4518319586424653 1102 56-57 0.4201786467791564 1102 58-59 0.42938756094974906 1102 60-61 0.47672156267245924 1102 62-63 0.48771031329280135 1102 64-65 0.43422419428633674 1102 66-67 0.4660524089596194 1102 68-69 0.5212689205656034 1102 70-71 0.32463388185760067 1102 72-73 0.49333314769887693 1102 74-75 0.3356113694830185 1102 76-77 0.3455772272974045 1102 78-79 0.5731707910288755 1102 80-81 0.5035432765076315 1102 82-83 0.4774707753298877 1102 84-85 0.4487372055061023 1102 86-87 0.4629758989329922 1102 88-89 0.5693335982156107 1102 90-91 0.59628855786411 1102 92-93 0.5363108924731712 1102 94-95 0.5368779227782063 1102 96-97 0.6174775997927249 1102 98-99 0.7138861813327466 1102 100-101 1.2728927952352258 1103 1 0.1326531498989496 1103 2 0.01533341698473123 1103 3 0.06201997760832256 1103 4 -0.04493052167941869 1103 5 0.013834947426929034 1103 6 -5.245921882348625E-4 1103 7 0.048681631038128614 1103 8 0.039066481947664045 1103 9 0.12884266138881628 1103 10-11 0.09534423902737643 1103 12-13 0.0433510426937076 1103 14-15 0.15209213123451804 1103 16-17 0.19033945294565058 1103 18-19 0.19908807660501537 1103 20-21 0.04982413563858756 1103 22-23 0.1365440503376547 1103 24-25 0.14842788710735277 1103 26-27 0.15364020558270397 1103 28-29 0.007172132879560422 1103 30-31 -0.01958869613065417 1103 32-33 0.09991344211885433 1103 34-35 0.11691993217297636 1103 36-37 0.15896401124200565 1103 38-39 0.14736429404620566 1103 40-41 0.22175124296951765 1103 42-43 0.3104980049259254 1103 44-45 0.17819734124483233 1103 46-47 0.15247203319889024 1103 48-49 0.06298299040546596 1103 50-51 0.05147978178005985 1103 52-53 0.10433598197352723 1103 54-55 0.03150482571070512 1103 56-57 0.010963403033599661 1103 58-59 0.07082896459446886 1103 60-61 0.20729669521429628 1103 62-63 0.2271399746532765 1103 64-65 0.08160429891310628 1103 66-67 0.23429283704214043 1103 68-69 0.20764421216380669 1103 70-71 0.2400495785868273 1103 72-73 0.4127934295704563 1103 74-75 0.30707022120459726 1103 76-77 0.19901369019957826 1103 78-79 0.32396067435258047 1103 80-81 0.2906418797360182 1103 82-83 0.3905905507013223 1103 84-85 0.35019860533791913 1103 86-87 0.3622103244575321 1103 88-89 0.3979899975816039 1103 90-91 0.4310775428709803 1103 92-93 0.5099876181083332 1103 94-95 0.24817117201361683 1103 96-97 0.43116014967985095 1103 98-99 0.40517661098855484 1103 100-101 0.7531427815075169 1104 1 0.015281323656566315 1104 2 -0.00446462282462079 1104 3 -0.017111564374047816 1104 4 -0.054323022531548304 1104 5 -0.011153325831848804 1104 6 0.02020365272325364 1104 7 1.4053920394019315E-4 1104 8 0.035457692382209416 1104 9 0.07866268209389915 1104 10-11 0.00943165736069318 1104 12-13 0.017332910834987558 1104 14-15 0.09247601301118635 1104 16-17 0.13547306162730877 1104 18-19 0.026419508270322467 1104 20-21 0.05207601905796366 1104 22-23 0.0832134019361419 1104 24-25 0.12178263642168474 1104 26-27 0.011942928789089535 1104 28-29 0.10479225501539702 1104 30-31 0.10801586228693338 1104 32-33 0.05580185305179697 1104 34-35 0.11213513612306514 1104 36-37 0.13535993776246613 1104 38-39 0.3747012161749339 1104 40-41 0.2332439254195222 1104 42-43 0.3088589587812791 1104 44-45 0.5036980365977399 1104 46-47 0.5783783881087956 1104 48-49 0.5850928961535615 1104 50-51 0.35956396550048453 1104 52-53 0.4158583700521703 1104 54-55 0.4961862296698101 1104 56-57 0.5515209484854324 1104 58-59 0.3593277449499084 1104 60-61 0.5171773395217656 1104 62-63 0.6539554297047872 1104 64-65 0.749052325170851 1104 66-67 0.6474974967635276 1104 68-69 0.6156581623840296 1104 70-71 0.5211156044243737 1104 72-73 0.4277763647023818 1104 74-75 0.44407172362389247 1104 76-77 0.4430273395550941 1104 78-79 0.5899698183544224 1104 80-81 0.6690192288133368 1104 82-83 0.6055766277747345 1104 84-85 0.6192302018440792 1104 86-87 0.5677102771697236 1104 88-89 0.5437651335895843 1104 90-91 0.7541840153339265 1104 92-93 0.595449061408349 1104 94-95 0.6973440797186115 1104 96-97 0.7736641437539582 1104 98-99 0.8851895786379842 1104 100-101 1.2308626079200096 1105 1 0.08890794132463498 1105 2 0.10585648490581434 1105 3 0.07955422529692413 1105 4 0.06507180800317514 1105 5 0.09657321842319533 1105 6 0.06408278857129801 1105 7 0.12658174725137172 1105 8 0.18655096653247938 1105 9 0.13639516728752454 1105 10-11 0.19744698073871092 1105 12-13 0.22759439700320883 1105 14-15 0.2785288817065421 1105 16-17 0.21515802214128144 1105 18-19 0.19311623325720717 1105 20-21 0.22853550963662883 1105 22-23 0.3590808850575087 1105 24-25 0.37151971452663446 1105 26-27 0.3290060470180123 1105 28-29 0.22292410566020493 1105 30-31 0.24207597044029683 1105 32-33 0.23290140903736045 1105 34-35 0.4301488045253308 1105 36-37 0.525017081953294 1105 38-39 0.40071050384225515 1105 40-41 0.46170448678163467 1105 42-43 0.4769037110229206 1105 44-45 0.46378730627250775 1105 46-47 0.3405817124882802 1105 48-49 0.3476068821131477 1105 50-51 0.22706335372525643 1105 52-53 0.2245597940385764 1105 54-55 0.3638638460520909 1105 56-57 0.3326664958226857 1105 58-59 0.3537692542877977 1105 60-61 0.5049119535517121 1105 62-63 0.5413721681577925 1105 64-65 0.40594013620373204 1105 66-67 0.5062741372759163 1105 68-69 0.6793365369432323 1105 70-71 0.7111069971359782 1105 72-73 0.7079625211838732 1105 74-75 0.5824038263342288 1105 76-77 0.6506148729198316 1105 78-79 0.7826684048353947 1105 80-81 0.6561003395991101 1105 82-83 0.74411277343523 1105 84-85 0.7956999476143416 1105 86-87 0.7061375056228592 1105 88-89 0.9608375875791388 1105 90-91 1.1959143702767179 1105 92-93 0.9898853016995126 1105 94-95 0.9983401417250342 1105 96-97 1.036016522038981 1105 98-99 0.9455580017007073 1105 100-101 1.0226406671851151 1106 1 0.11819198223658134 1106 2 0.24756624434813546 1106 3 -0.08935755117298783 1106 4 -0.0010886971108163834 1106 5 0.10481739018480596 1106 6 0.0711698835944361 1106 7 0.14207907597543823 1106 8 0.1432873673095756 1106 9 0.1094449215872757 1106 10-11 0.08815411890224567 1106 12-13 -0.022355605853768168 1106 14-15 0.02952436053632823 1106 16-17 -0.02038030424006365 1106 18-19 -0.034940377793596156 1106 20-21 0.021393774878610827 1106 22-23 0.09722174129429817 1106 24-25 0.09345613958806354 1106 26-27 0.17592729437529897 1106 28-29 0.3430645619198458 1106 30-31 0.3657680094666418 1106 32-33 0.4446321672797424 1106 34-35 0.5812830254269912 1106 36-37 0.4222613465667422 1106 38-39 0.2951085025280449 1106 40-41 0.3893479071576209 1106 42-43 0.5340379462094873 1106 44-45 0.6338695515727721 1106 46-47 0.5022635449549995 1106 48-49 0.685810770462389 1106 50-51 0.6224675449143362 1106 52-53 0.7687179849990926 1106 54-55 0.9254558622225844 1106 56-57 0.7450215041079247 1106 58-59 0.7151405687841077 1106 60-61 0.9397326423694992 1106 62-63 0.8994043877452427 1106 64-65 0.9401908575910838 1106 66-67 0.9185657563262595 1106 68-69 0.9853261946770786 1106 70-71 0.874353025934326 1106 72-73 1.0008484634157426 1106 74-75 0.9960998294197765 1106 76-77 0.8515266065350531 1106 78-79 0.7419126980186412 1106 80-81 0.8038767166632788 1106 82-83 0.5581635776749891 1106 84-85 0.7924965592365361 1106 86-87 0.6519745292215084 1106 88-89 0.7301838035096573 1106 90-91 0.962802690944109 1106 92-93 1.0220834618046517 1106 94-95 1.1180975841568959 1106 96-97 1.2255396207190579 1106 98-99 1.3586201124372366 1106 100-101 1.6588935955892055 1107 1 0.11160774123962369 1107 2 -0.011504259037209863 1107 3 0.08990110842862009 1107 4 0.22151703343574525 1107 5 0.12045434926635323 1107 6 0.024541118016969676 1107 7 -0.04393370490965509 1107 8 0.0924845756306425 1107 9 0.21601137728347908 1107 10-11 0.27429906297054174 1107 12-13 0.2320330704292317 1107 14-15 0.3559996205696905 1107 16-17 0.23359072411828663 1107 18-19 0.18551455793400606 1107 20-21 0.2962085624362629 1107 22-23 0.38351510063096583 1107 24-25 0.40364865013658147 1107 26-27 0.28726266381892884 1107 28-29 0.3180342042513189 1107 30-31 0.24788672941561884 1107 32-33 0.19561587839739758 1107 34-35 0.240617241892501 1107 36-37 0.296624448813823 1107 38-39 0.23774385479965332 1107 40-41 0.2413533531393668 1107 42-43 0.14060072218828878 1107 44-45 0.2906448312312975 1107 46-47 0.32538872894086524 1107 48-49 0.2558339526819893 1107 50-51 0.24865180063015657 1107 52-53 0.09719531793991365 1107 54-55 0.16805319074776293 1107 56-57 0.20705172685349282 1107 58-59 0.3411558641427561 1107 60-61 0.22871403526519174 1107 62-63 0.14214399867645966 1107 64-65 0.3462510455019867 1107 66-67 0.4288103962045895 1107 68-69 0.2912161956583361 1107 70-71 0.46535673018317425 1107 72-73 0.4130120759169671 1107 74-75 0.473015122325279 1107 76-77 0.3691898633302628 1107 78-79 0.41231519777332437 1107 80-81 0.25368506471548 1107 82-83 0.3703646006364245 1107 84-85 0.28644342411731216 1107 86-87 0.357625961858151 1107 88-89 0.412434379998718 1107 90-91 0.37813116705047634 1107 92-93 0.5140028023992933 1107 94-95 0.45215704839004545 1107 96-97 0.41189461532213656 1107 98-99 0.6483757792020306 1107 100-101 0.9585489287262909 1108 1 -0.01522216280547184 1108 2 -0.07268569363636601 1108 3 -0.02805663231393396 1108 4 -0.10659661366524631 1108 5 0.06635793820791491 1108 6 -0.0138685393800273 1108 7 -0.006301135631517241 1108 8 4.330304145412356E-4 1108 9 0.06122259211494452 1108 10-11 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-0.5290976296188532 2316 54-55 -0.8457020835138493 2316 56-57 -0.7531291049729134 2316 58-59 -0.7843534788349444 2316 60-61 -0.7625216488818864 2316 62-63 -0.5095497927641688 2316 64-65 -0.6757622154986151 2316 66-67 -0.5678261318243472 2316 68-69 -0.2901942925875929 2316 70-71 -0.5935221124931402 2316 72-73 -0.9935099252885706 2316 74-75 -1.0075394286090216 2316 76-77 -0.9303158630109039 2316 78-79 -0.8909036087366253 2316 80-81 -0.9417579732592039 2316 82-83 -0.6653074909850325 2316 84-85 -0.8632371063227104 2316 86-87 -0.8721630676777146 2316 88-89 -0.8614655597240066 2316 90-91 -1.044713920351576 2316 92-93 -1.0867205249063154 2316 94-95 -1.0834306550457597 2316 96-97 -1.6389191278966706 2316 98-99 -1.3857652696225635 2316 100-101 -1.521740402195956 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5112.0 3 829.0 4 637.0 5 660.0 6 693.0 7 826.0 8 955.0 9 1177.0 10 1527.0 11 1990.0 12 2884.0 13 3932.0 14 4867.0 15 6147.0 16 8800.0 17 12627.0 18 10155.0 19 5786.0 20 5390.0 21 5585.0 22 5618.0 23 6136.0 24 6841.0 25 7506.0 26 8783.0 27 10372.0 28 12730.0 29 15868.0 30 20147.0 31 26251.0 32 35512.0 33 51045.0 34 77247.0 35 121769.0 36 199976.0 37 302393.0 38 248814.0 39 29501.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.96760495937759 19.046748425955606 15.1405856117898 23.845061002877006 2 14.566953120116596 19.957320783291017 41.26828643024037 24.20743966635202 3 18.125753189011178 24.413966527746236 30.550256252408424 26.910024030834162 4 12.006133862529317 16.606689011903743 38.054714180363405 33.33246294520353 5 13.133156005082498 36.89920297227888 35.03939250569249 14.928248516946136 6 30.82469669977409 36.66060184822772 17.99843595686151 14.516265495136679 7 27.42817062234994 32.92811764091875 21.451390723030475 18.19232101370084 8 25.713944554536873 34.47620342971736 20.423471881093853 19.386380134651912 9 25.683433440557184 16.395918535176698 20.87140728932411 37.04924073494201 10-11 23.89979980363912 26.65913277767607 28.224919010034334 21.216148408650476 12-13 24.781647172514255 24.15156843540793 28.830995366242412 22.235789025835402 14-15 21.850703287649353 25.367181069389417 27.248096984034987 25.534018658926243 16-17 21.098565409051965 28.789598731274857 27.789184758691942 22.32265110098124 18-19 20.884228603823978 26.976163677687598 30.722261163355487 21.417346555132934 20-21 21.484757695875054 26.084965971387025 30.923813387039 21.50646294569892 22-23 21.196462861189005 26.067560189437057 30.817244797063637 21.9187321523103 24-25 21.069331073110412 26.265564070045826 30.89635918568722 21.768745671156537 26-27 20.799677696622062 26.640082173128153 30.679035335552136 21.881204794697652 28-29 20.717395464170554 26.62364412984725 30.876201681682254 21.782758724299946 30-31 20.904793139700438 26.37980340966915 30.861621710101023 21.85378174052939 32-33 20.655272964504235 26.46436090820129 30.81783585556555 22.06253027172892 34-35 21.197252479631597 26.643902446738526 30.444277870553112 21.71456720307677 36-37 21.04925242316577 26.403641281722944 30.510559851413692 22.036546443697596 38-39 20.819971283452567 26.606213274129175 30.603403290385017 21.970412152033244 40-41 21.09950477494676 26.550815024132685 30.555785064341308 21.79389513657925 42-43 20.893932266238668 26.508196915730093 30.37966326825084 22.2182075497804 44-45 20.967446045867636 26.789305090227327 30.12496056186785 22.118288302037183 46-47 21.028506052302934 26.882985910838087 29.797041588308986 22.29146644854999 48-49 21.255847843972624 27.090427544457057 29.437709515207654 22.216015096362664 50-51 21.209580009686764 27.204301712975322 29.24540125927903 22.340717018058893 52-53 21.35783480289431 27.272231228500253 29.13257680597841 22.23735716262702 54-55 21.1452074055417 27.18661615552539 29.28587837706928 22.382298061863626 56-57 21.204350534764433 27.5629004408407 29.116595090666447 22.116153933728423 58-59 21.35542760381937 27.155825793979083 29.20733122077261 22.28141538142894 60-61 21.29619893697678 27.161879794415768 29.497550366318464 22.044370902288986 62-63 21.125335808446483 27.207401937608804 29.53533123876356 22.13193101518115 64-65 21.094343247398086 27.15231761901167 29.355519250146163 22.397819883444086 66-67 21.016617481661868 27.45321824108098 29.163512303498823 22.366651973758334 68-69 20.964473125080293 27.22095838562718 29.410283523238235 22.404284966054295 70-71 21.107838343093 27.272022893475683 29.29350509501591 22.326633668415408 72-73 21.189812563503455 27.282467314253633 29.145287994338553 22.382432127904355 74-75 21.037932089565807 27.44160599234828 29.015435483348128 22.505026434737783 76-77 21.04334102538456 27.325297070391958 29.119283196235074 22.512078707988415 78-79 21.196642017477796 27.35969037297959 29.098495944842746 22.34517166469987 80-81 21.01544204962138 27.544119577059245 28.985032311927505 22.455406061391873 82-83 21.068761803627957 27.52041679235459 29.008746298054135 22.40207510596332 84-85 21.06298605659424 27.338111580450295 28.87776020061681 22.721142162338655 86-87 21.01949728370973 27.268644922377305 29.072903625950048 22.638954167962915 88-89 21.10715700405364 27.19232219761557 29.022390295930784 22.678130502400005 90-91 20.954686959529575 27.32994020852893 29.09826555319464 22.61710727874685 92-93 21.075423720772836 27.115203977393126 29.106391154601546 22.702981147232492 94-95 20.92657440797864 27.05184392189295 29.049666462941687 22.971915207186722 96-97 21.07656396076238 26.961851433857177 29.012833461476884 22.94875114390356 98-99 20.983012025950217 27.03480730056337 28.909560474270172 23.072620199216242 100-101 20.94038479630082 26.838225702786477 29.302909491362755 22.918480009549942 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1728.0 1 1015.5 2 326.5 3 353.0 4 290.5 5 284.0 6 510.5 7 1067.5 8 1771.0 9 2122.5 10 1881.5 11 1565.5 12 1615.5 13 1712.0 14 1819.0 15 1988.0 16 2060.5 17 2182.0 18 2313.0 19 2452.5 20 2682.5 21 3026.0 22 3606.5 23 4298.5 24 5136.5 25 6380.0 26 8185.5 27 10474.0 28 12281.0 29 14431.5 30 17183.5 31 19803.5 32 22805.5 33 25809.0 34 29477.0 35 32303.0 36 34831.0 37 37884.0 38 39413.5 39 39748.0 40 40088.0 41 41496.5 42 43352.0 43 45216.0 44 47170.5 45 48556.0 46 47763.5 47 46295.0 48 46648.5 49 46342.0 50 44534.5 51 42407.5 52 39883.5 53 38346.0 54 36887.0 55 34026.5 56 30464.0 57 26387.0 58 23396.5 59 20865.5 60 17798.0 61 14642.5 62 11401.5 63 8689.0 64 6807.0 65 5381.0 66 3910.5 67 2921.0 68 2130.5 69 1386.0 70 1004.0 71 751.5 72 604.5 73 474.5 74 332.5 75 265.5 76 179.5 77 97.5 78 63.0 79 45.5 80 30.5 81 19.5 82 11.5 83 11.0 84 11.5 85 7.5 86 4.0 87 5.0 88 5.5 89 3.5 90 4.0 91 3.0 92 2.0 93 1.5 94 1.5 95 1.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2862459868738813 2 0.2560982154413413 3 0.2603599422930616 4 0.25949181274919264 5 0.2482850495465207 6 0.19106742051879422 7 0.19714432732587686 8 0.19714432732587686 9 0.19319828394465438 10-11 0.20389206150776737 12-13 0.2026293276257762 14-15 0.1885419527548118 16-17 0.19852544250930476 18-19 0.18645054976276387 20-21 0.19106742051879422 22-23 0.18921278012961962 24-25 0.18309641288872472 26-27 0.19296152134178102 28-29 0.18988360750442748 30-31 0.1959210538776979 32-33 0.19765731296543582 34-35 0.18846303188718735 36-37 0.18723975843900836 38-39 0.18278072941822693 40-41 0.18258342724916582 42-43 0.18049202425711786 44-45 0.19359288828277663 46-47 0.1835304776606592 48-49 0.19071227661448417 50-51 0.1956842912748246 52-53 0.2006168455013527 54-55 0.20144551461140944 56-57 0.17646706000827092 58-59 0.16719385806239798 60-61 0.17433619658241076 62-63 0.176309218273022 64-65 0.17405997354572517 66-67 0.182267743778668 68-69 0.17398105267810074 70-71 0.1797422760146856 72-73 0.1891733196958074 74-75 0.17986065731612227 76-77 0.18676623323326166 78-79 0.18502997414552377 80-81 0.176309218273022 82-83 0.18826572971812622 84-85 0.17307346270041954 86-87 0.17548054916296527 88-89 0.18175475813910907 90-91 0.1799001177499345 92-93 0.18499051371171155 94-95 0.1827018085506025 96-97 0.1763486787068342 98-99 0.1803736429556812 100-101 0.170705836671686 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1267092.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.97317304656827 #Duplication Level Percentage of deduplicated Percentage of total 1 78.77305339157684 46.45496909068022 2 12.242298070536105 14.439343252027893 3 3.8693548565500397 6.845644006017146 4 1.6788159539592962 3.9602041506472467 5 0.8985136539688792 2.649410060010554 6 0.537685005011652 1.9025394507058246 7 0.35851739157979573 1.48000357216877 8 0.24939607255692578 1.1766142195227265 9 0.19541927398101822 1.037204519500559 >10 1.0788508108547863 11.619009464372052 >50 0.08118971584965373 3.277515871111473 >100 0.03596979308343213 3.656273419715552 >500 2.674315690126897E-4 0.10961045297262928 >1k 5.348631380253794E-4 0.8658437220427839 >5k 1.3371578450634486E-4 0.5258147485046706 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6668 0.5262443453198348 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4771 0.37653145943625244 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3376 0.26643684910014426 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1666 0.1314821654623342 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 7.892086762445032E-5 9 0.0 0.0 0.0 0.0 7.892086762445032E-5 10-11 3.946043381222516E-5 0.0 0.0 0.0 7.892086762445032E-5 12-13 7.892086762445032E-5 0.0 0.0 0.0 7.892086762445032E-5 14-15 7.892086762445032E-5 0.0 0.0 0.0 1.5784173524890064E-4 16-17 7.892086762445032E-5 0.0 0.0 0.0 2.3676260287335094E-4 18-19 7.892086762445032E-5 0.0 0.0 0.0 2.3676260287335094E-4 20-21 7.892086762445032E-5 0.0 0.0 3.946043381222516E-5 2.3676260287335094E-4 22-23 7.892086762445032E-5 0.0 0.0 2.3676260287335097E-4 3.1568347049780123E-4 24-25 7.892086762445032E-5 0.0 0.0 6.708273748078277E-4 3.9460433812225156E-4 26-27 7.892086762445032E-5 0.0 0.0 0.001262733881991205 3.9460433812225156E-4 28-29 7.892086762445032E-5 0.0 0.0 0.0025254677639824103 3.9460433812225156E-4 30-31 7.892086762445032E-5 0.0 0.0 0.005642842035148197 4.340647719344767E-4 32-33 7.892086762445032E-5 0.0 0.0 0.009470504114934039 5.524460733711522E-4 34-35 7.892086762445032E-5 0.0 0.0 0.013929533135715481 6.313669409956026E-4 36-37 7.892086762445032E-5 0.0 0.0 0.01980913777373703 6.313669409956026E-4 38-39 7.892086762445032E-5 0.0 0.0 0.0304239944692256 6.313669409956026E-4 40-41 7.892086762445032E-5 0.0 0.0 0.047391981008482416 6.313669409956026E-4 42-43 7.892086762445032E-5 0.0 0.0 0.064320507113927 6.708273748078277E-4 44-45 7.892086762445032E-5 0.0 0.0 0.08645781048258533 7.49748242432278E-4 46-47 7.892086762445032E-5 0.0 0.0 0.12063054616397231 7.892086762445031E-4 48-49 7.892086762445032E-5 0.0 0.0 0.15117292193463458 8.681295438689535E-4 50-51 7.892086762445032E-5 0.0 0.0 0.18408292373403035 8.681295438689535E-4 52-53 7.892086762445032E-5 0.0 0.0 0.21501990384281489 9.075899776811786E-4 54-55 7.892086762445032E-5 0.0 0.0 0.2497056251637608 9.470504114934038E-4 56-57 7.892086762445032E-5 0.0 0.0 0.31039577236696314 9.470504114934038E-4 58-59 7.892086762445032E-5 0.0 0.0 0.368915595710493 9.470504114934038E-4 60-61 7.892086762445032E-5 0.0 0.0 0.4247126491209794 9.470504114934038E-4 62-63 7.892086762445032E-5 0.0 0.0 0.4853633358903694 9.470504114934038E-4 64-65 7.892086762445032E-5 0.0 0.0 0.5551688433041957 9.470504114934038E-4 66-67 7.892086762445032E-5 0.0 0.0 0.6389038838537375 9.470504114934038E-4 68-69 7.892086762445032E-5 0.0 0.0 0.7385809396634183 9.470504114934038E-4 70-71 7.892086762445032E-5 0.0 0.0 0.8569227806662816 9.470504114934038E-4 72-73 7.892086762445032E-5 0.0 0.0 0.9775138663964417 0.0010259712791178541 74-75 7.892086762445032E-5 0.0 0.0 1.1200054928923868 0.0010259712791178541 76-77 7.892086762445032E-5 0.0 0.0 1.283766293213121 0.0010259712791178541 78-79 7.892086762445032E-5 0.0 0.0 1.4717163394607495 0.0010259712791178541 80-81 7.892086762445032E-5 0.0 0.0 1.6844869985762676 0.0010259712791178541 82-83 7.892086762445032E-5 3.946043381222516E-5 0.0 1.9067281618067198 0.0011048921467423044 84-85 7.892086762445032E-5 7.892086762445032E-5 0.0 2.139663102600285 0.0011048921467423044 86-87 7.892086762445032E-5 7.892086762445032E-5 0.0 2.387356245639622 0.0011048921467423044 88-89 7.892086762445032E-5 7.892086762445032E-5 0.0 2.6638160449280717 0.0011838130143667547 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2400 0.0 58.834488 1 GTATCAA 4845 0.0 46.807056 1 ATCAACG 6285 0.0 36.1441 3 TATCAAC 6315 0.0 35.97382 2 TCAACGC 6435 0.0 35.30438 4 CAACGCA 6595 0.0 34.519928 5 GTGGTAT 930 0.0 34.25142 1 AACGCAG 6780 0.0 33.564728 6 TGGTATC 980 0.0 31.522404 2 ACGCAGA 7630 0.0 29.45194 7 CGCAGAG 7590 0.0 29.168997 8 GCAGAGT 7945 0.0 27.564491 9 GTACATG 4765 0.0 27.43826 1 TATCACG 70 0.008369441 27.15776 2 TACATGG 4885 0.0 25.781805 2 AGTACGT 95 0.0012227029 25.01373 2 ACATGGG 4860 0.0 24.642178 3 GAGTACT 5185 0.0 22.675707 12-13 CAGAGTA 7810 0.0 22.566587 10-11 AGAGTAC 7640 0.0 22.167114 10-11 >>END_MODULE