FastQCFastQC Report
Thu 26 May 2016
SRR937441_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937441_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267092
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63960.5047778693259842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54940.43359124672873006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39420.3111060601755832No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14400.11364604937920847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32000.063.4020161
GTATCAA58150.041.1001471
TGGTATC11750.033.1511232
GTGGTAT12350.032.702211
TATCAAC78600.030.2787192
ATCAACG79450.029.7156183
TCAACGC79850.029.5667634
CAACGCA81700.028.8391135
AACGCAG84700.027.8143656
ATACCGT1551.4114703E-624.5148476
ACGCAGA97800.024.042057
TACCGTG1601.8593764E-623.7506317
CGCAGAG98950.023.4746028
GCAGAGT102850.022.2149819
GAGTACT69050.021.80724112-13
AGTACTT69750.021.48623312-13
GTACTTT70850.021.11745614-15
AGAGTAC98000.020.69697810-11
CAGAGTA101250.020.52523610-11
ATACGTC3102.582965E-1019.920675
Produced by FastQC (version 0.11.5)