##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937441_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1267092 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.026485843174765 31.0 31.0 33.0 30.0 34.0 2 31.29469999021381 31.0 31.0 34.0 30.0 34.0 3 31.28892377191238 31.0 31.0 34.0 28.0 34.0 4 35.10836466491778 35.0 35.0 37.0 32.0 37.0 5 34.87195720594874 35.0 35.0 37.0 32.0 37.0 6 35.075943972497654 36.0 35.0 37.0 32.0 37.0 7 35.01977993705272 36.0 35.0 37.0 32.0 37.0 8 34.99997079927898 36.0 35.0 37.0 32.0 37.0 9 36.462125875627024 38.0 35.0 39.0 32.0 39.0 10-11 36.329682848601365 38.0 35.0 39.0 32.0 39.0 12-13 36.194698964242534 38.0 35.0 39.0 32.0 39.0 14-15 37.01260050572492 38.0 36.0 40.0 31.0 41.0 16-17 36.93797096027755 38.0 36.0 40.0 31.0 41.0 18-19 36.91798701278202 38.5 36.0 40.0 31.0 41.0 20-21 36.768007374365865 38.0 36.0 40.0 31.0 41.0 22-23 36.56542500465633 38.0 35.0 40.0 30.0 41.0 24-25 36.350309211959356 38.0 35.0 40.0 30.0 41.0 26-27 36.187789047677676 38.0 35.0 40.0 30.0 41.0 28-29 36.00953245699602 38.0 34.0 40.0 29.0 41.0 30-31 36.04875731201838 38.0 35.0 40.0 30.0 41.0 32-33 35.91426194783015 38.0 34.5 40.0 30.0 41.0 34-35 35.71345253541179 38.0 34.0 40.0 29.0 41.0 36-37 35.42974306522336 38.0 34.0 40.0 27.5 41.0 38-39 35.20970892405603 38.0 34.0 40.0 27.0 41.0 40-41 34.91764449621653 38.0 33.0 40.0 26.5 41.0 42-43 34.582657376102134 37.5 33.0 40.0 25.5 41.0 44-45 34.110216937680924 37.0 33.0 40.0 24.0 41.0 46-47 33.59210065251773 37.0 32.5 40.0 23.0 40.5 48-49 33.16507049211896 37.0 31.5 39.0 21.0 40.0 50-51 32.79449716358401 37.0 31.0 39.0 19.0 40.0 52-53 32.4111674606106 36.0 31.0 39.0 17.5 40.0 54-55 32.01505533931238 36.0 30.0 39.0 14.5 40.0 56-57 31.634996117093316 35.5 30.0 39.0 13.0 40.0 58-59 31.265961350872708 35.0 29.0 38.5 9.5 40.0 60-61 30.75856094111556 34.5 28.5 38.0 8.0 40.0 62-63 30.195608132637567 34.0 28.0 38.0 7.0 40.0 64-65 29.670134844194422 34.0 27.5 38.0 4.5 39.5 66-67 29.073453624519765 33.5 26.0 37.0 2.0 39.0 68-69 28.515474014515128 33.0 26.0 36.5 2.0 39.0 70-71 27.98817055115177 32.5 25.5 36.0 2.0 39.0 72-73 27.375649913344887 32.0 24.5 35.5 2.0 38.0 74-75 26.76767196067847 31.0 23.0 35.0 2.0 37.5 76-77 25.97851497760226 30.5 23.0 34.0 2.0 36.0 78-79 26.577014928671318 31.5 24.0 34.5 2.0 36.0 80-81 26.32921918850407 31.5 23.5 35.0 2.0 36.0 82-83 25.8289524359715 31.0 22.5 34.0 2.0 36.0 84-85 25.21310212675954 31.0 20.0 34.0 2.0 35.5 86-87 24.522265944382887 30.5 18.0 34.0 2.0 35.0 88-89 23.78526736811534 30.0 13.0 34.0 2.0 35.0 90-91 22.926601225483232 29.0 6.0 34.0 2.0 35.0 92-93 22.09546938975228 29.0 2.0 34.0 2.0 35.0 94-95 21.16942061034242 29.0 2.0 33.0 2.0 34.5 96-97 20.114944692255968 27.0 2.0 33.0 2.0 34.0 98-99 18.97398768203098 25.5 2.0 32.5 2.0 34.0 100-101 16.927640613309848 21.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1944944939567783 1101 2 0.25016068372293887 1101 3 0.08274808988302951 1101 4 0.11076446805340368 1101 5 0.11955272128626149 1101 6 0.07678021216487707 1101 7 0.17920729462413476 1101 8 0.2333281665566389 1101 9 0.33867672237143154 1101 10-11 0.16251544367248272 1101 12-13 0.1357977334463314 1101 14-15 0.24088878027696836 1101 16-17 0.11270242465683111 1101 18-19 0.06313637773192937 1101 20-21 0.20137300711459005 1101 22-23 0.12984115420280773 1101 24-25 0.20959947081045271 1101 26-27 0.17947949513516903 1101 28-29 0.1707611856457163 1101 30-31 0.2924214897812547 1101 32-33 0.1391636601648898 1101 34-35 0.21674913266343054 1101 36-37 0.14356130792011612 1101 38-39 -0.034709007413390225 1101 40-41 -0.0900036337795811 1101 42-43 0.026177965711269735 1101 44-45 0.07646221706309575 1101 46-47 0.04495259245687322 1101 48-49 0.17850812540252292 1101 50-51 0.03422465713365597 1101 52-53 0.0846955924938726 1101 54-55 -0.015889213835500016 1101 56-57 -0.015319228864672851 1101 58-59 -0.28729414343780135 1101 60-61 -0.26070927235338104 1101 62-63 0.22299421426463795 1101 64-65 -0.06888957328620293 1101 66-67 0.15803872162487664 1101 68-69 0.23889057744170472 1101 70-71 -0.26945507976924077 1101 72-73 -0.19540452680361398 1101 74-75 -0.6169179180336428 1101 76-77 -0.3140414747996658 1101 78-79 -0.33037393729743414 1101 80-81 -0.19801921765057884 1101 82-83 -0.3929096517663204 1101 84-85 -0.17913303745389086 1101 86-87 0.03624605091412647 1101 88-89 0.005859056794566442 1101 90-91 -0.08968302890500368 1101 92-93 -0.22612233183713215 1101 94-95 -0.05027246201326818 1101 96-97 -0.027268444786500368 1101 98-99 0.23618987858999674 1101 100-101 0.6029171664093091 1102 1 -0.017374377778406114 1102 2 0.12631473469600252 1102 3 0.17792963790860838 1102 4 0.2498876064803497 1102 5 -0.01669721025908899 1102 6 0.07619669528914841 1102 7 0.11746057116265973 1102 8 0.11205170608062076 1102 9 0.162002353114147 1102 10-11 0.11465069369403835 1102 12-13 0.14328399687996551 1102 14-15 0.09391900796185126 1102 16-17 0.0579497206194759 1102 18-19 0.06579399180883883 1102 20-21 0.04827347499818302 1102 22-23 0.1621931363661986 1102 24-25 0.16763818687189058 1102 26-27 0.1762753454300423 1102 28-29 0.20075785575976823 1102 30-31 0.066958783559663 1102 32-33 0.09033346357572469 1102 34-35 0.24527347702525049 1102 36-37 0.2600226526023377 1102 38-39 0.16268084040011388 1102 40-41 0.3632434141726648 1102 42-43 0.08453830846922017 1102 44-45 0.1317611419946303 1102 46-47 0.2899853027637249 1102 48-49 0.45915346233473286 1102 50-51 0.48045612638130564 1102 52-53 0.4819221877704649 1102 54-55 0.4823928105085429 1102 56-57 0.40733520010395097 1102 58-59 0.6126035994786392 1102 60-61 0.33616884941469394 1102 62-63 0.23911457610804732 1102 64-65 0.22225387429768162 1102 66-67 0.5158714757426992 1102 68-69 0.7074598217590271 1102 70-71 0.41893022769127697 1102 72-73 0.3571459832858146 1102 74-75 0.2502437098462522 1102 76-77 0.4042220054158392 1102 78-79 0.29494622588585884 1102 80-81 0.2821009226200779 1102 82-83 0.5897418942386636 1102 84-85 0.4212515360468885 1102 86-87 0.5367977796536856 1102 88-89 0.6095760341142018 1102 90-91 0.5921792585944559 1102 92-93 0.671058784373372 1102 94-95 0.6947432526564192 1102 96-97 0.6288633922593299 1102 98-99 0.7629345594276664 1102 100-101 0.9645846398441869 1103 1 0.07242244590655744 1103 2 0.04936088017026563 1103 3 0.07577879842042634 1103 4 0.24276677978208028 1103 5 0.15488099543942724 1103 6 0.0722758138034223 1103 7 0.12222516711443632 1103 8 0.1507018493115666 1103 9 -0.027292622419679446 1103 10-11 0.09187749833296266 1103 12-13 0.10300380347037219 1103 14-15 0.20117547679932812 1103 16-17 0.2720892336651275 1103 18-19 0.2450529297836468 1103 20-21 0.2044846633615265 1103 22-23 -0.026028763931734034 1103 24-25 0.035707928587299875 1103 26-27 0.005332162118499184 1103 28-29 -0.04345259716836125 1103 30-31 -0.12494754223197191 1103 32-33 -0.07778134822504512 1103 34-35 0.04221330769006215 1103 36-37 -0.02431868558124961 1103 38-39 0.23896388793995982 1103 40-41 0.28006797524061966 1103 42-43 0.3608856448919511 1103 44-45 0.18879778233905142 1103 46-47 0.36992161851714656 1103 48-49 0.678261636092401 1103 50-51 0.47918746980301563 1103 52-53 0.6631827496256903 1103 54-55 0.5870434818708077 1103 56-57 0.24412611747916202 1103 58-59 0.33258957561771396 1103 60-61 0.5168457521219239 1103 62-63 0.18618514395410202 1103 64-65 0.2980306931578909 1103 66-67 0.4250114484353489 1103 68-69 0.5516430352036714 1103 70-71 0.2741381824842257 1103 72-73 0.6470050906821854 1103 74-75 0.6363738769418248 1103 76-77 0.4710452708345265 1103 78-79 0.5292117994358598 1103 80-81 0.6743064508470304 1103 82-83 0.6114967709699748 1103 84-85 0.5932202874543648 1103 86-87 0.6836792135549068 1103 88-89 0.589028270620787 1103 90-91 0.8559904170961659 1103 92-93 0.7785913499008288 1103 94-95 0.860076120378654 1103 96-97 1.0490989042783276 1103 98-99 1.0072323144376618 1103 100-101 0.8486167805837788 1104 1 0.16877944408931 1104 2 0.16590437638311784 1104 3 0.08001883259459319 1104 4 0.14261152344091244 1104 5 -0.008469812166488566 1104 6 0.01503598718781518 1104 7 -0.07000928646385063 1104 8 0.061676047941929824 1104 9 0.08053055799948794 1104 10-11 0.10688994867283697 1104 12-13 0.16224844265171612 1104 14-15 0.16860798377031472 1104 16-17 0.11516940902989603 1104 18-19 0.09398679604103677 1104 20-21 -0.041949233045805556 1104 22-23 0.1162588170208636 1104 24-25 0.09844853307145485 1104 26-27 0.05191334893453359 1104 28-29 -0.10322755798930672 1104 30-31 0.025548598892619623 1104 32-33 -0.08455270819061411 1104 34-35 0.04650713571097498 1104 36-37 -0.1549593433197245 1104 38-39 -0.25550232793744954 1104 40-41 -0.1511276318808612 1104 42-43 -0.04264358055917228 1104 44-45 0.004557506965333857 1104 46-47 0.1425786663997073 1104 48-49 -0.03303424656782994 1104 50-51 -0.2022648878908342 1104 52-53 -0.18538058969320304 1104 54-55 0.23251934001754648 1104 56-57 0.07633803159435004 1104 58-59 0.2455973086021963 1104 60-61 0.36645957894953085 1104 62-63 0.31986682021480206 1104 64-65 -0.043124011597992506 1104 66-67 0.2580942198689442 1104 68-69 0.1486863413131907 1104 70-71 0.18126286805204828 1104 72-73 0.22267145204099847 1104 74-75 -0.1498822236612405 1104 76-77 0.12039142963650207 1104 78-79 0.20894465824081365 1104 80-81 0.2617477933383334 1104 82-83 0.31242964196185596 1104 84-85 0.23579841865295492 1104 86-87 0.4776889275493694 1104 88-89 0.6135458873915205 1104 90-91 0.4507120188642837 1104 92-93 0.3249383192602373 1104 94-95 0.5629773066361174 1104 96-97 0.6724813598039887 1104 98-99 0.7805190200897947 1104 100-101 1.155511628370995 1105 1 -0.19320335830800062 1105 2 0.03755534907035951 1105 3 0.002594257760691221 1105 4 0.06046708507885512 1105 5 -0.0264482239272823 1105 6 0.06964205345191488 1105 7 0.06640421134861185 1105 8 0.028588556305216173 1105 9 -0.023146600081815905 1105 10-11 -0.05785404638204028 1105 12-13 -0.07753102122101296 1105 14-15 -0.09280303865107697 1105 16-17 0.09389695900097195 1105 18-19 0.19582314722971716 1105 20-21 0.08823688807295582 1105 22-23 -0.07637293390104105 1105 24-25 -0.16494174132918715 1105 26-27 0.11831872039171287 1105 28-29 0.04264847584009601 1105 30-31 0.04889012223414824 1105 32-33 -0.05678677692728229 1105 34-35 -0.22369930483225176 1105 36-37 -0.22403106459351818 1105 38-39 -0.27319854511552677 1105 40-41 -0.053378681800303696 1105 42-43 -0.10158547110728477 1105 44-45 -0.04709612499917881 1105 46-47 0.10306629722412453 1105 48-49 0.14786503472005563 1105 50-51 0.20830701231837168 1105 52-53 0.3445857874234264 1105 54-55 0.19213722450589898 1105 56-57 -0.21815977520915553 1105 58-59 -0.16042340829934787 1105 60-61 -0.11033256882706866 1105 62-63 0.15013296719079605 1105 64-65 0.058533227364943485 1105 66-67 0.09265039639402772 1105 68-69 0.28707608527288286 1105 70-71 -0.05799464193085058 1105 72-73 0.0892211741606701 1105 74-75 0.10505352933150647 1105 76-77 0.2168057902995706 1105 78-79 0.4190411772751581 1105 80-81 0.05623251593186396 1105 82-83 -0.010214245448867842 1105 84-85 -0.13794991753061225 1105 86-87 0.19281915433762364 1105 88-89 0.17270755730448073 1105 90-91 0.2865668916362587 1105 92-93 0.1266350937549383 1105 94-95 0.2762725613821466 1105 96-97 0.6246808508635837 1105 98-99 0.7546916736665956 1105 100-101 1.0376041542459014 1106 1 -0.2154628006255841 1106 2 -0.021907324491152735 1106 3 -0.006637357575574043 1106 4 -0.004097082682989139 1106 5 -0.015830973484447952 1106 6 -0.21640588666579674 1106 7 -0.017074946985204065 1106 8 -0.08217118599522166 1106 9 -0.17002213576548542 1106 10-11 -0.3627759079899491 1106 12-13 -0.4262530585331632 1106 14-15 -0.16134287208915765 1106 16-17 0.005137745241754033 1106 18-19 -0.05275193419419821 1106 20-21 -0.11962801964659775 1106 22-23 -0.09834221422788403 1106 24-25 0.007798387806296603 1106 26-27 -0.17753133817636524 1106 28-29 -0.05633247524841778 1106 30-31 -0.003157796456214612 1106 32-33 -0.24545813856376242 1106 34-35 -0.15265068618188593 1106 36-37 -0.06697664627456135 1106 38-39 -0.01752833341333826 1106 40-41 0.0629441354672835 1106 42-43 -0.20174135505676816 1106 44-45 -0.18305085615739358 1106 46-47 0.12137150121247231 1106 48-49 0.1594259812373977 1106 50-51 -0.1610745587347111 1106 52-53 -0.10972932314167849 1106 54-55 -0.109546429285313 1106 56-57 0.24437450854605558 1106 58-59 0.13477133057271473 1106 60-61 0.07099099839766154 1106 62-63 0.10292405769990509 1106 64-65 -0.0065416255006063295 1106 66-67 -0.11810363194720708 1106 68-69 0.4475499510519789 1106 70-71 0.2594041852790241 1106 72-73 0.09598596617255595 1106 74-75 -0.27493368568477905 1106 76-77 -0.10532937340536463 1106 78-79 -0.13267908156719344 1106 80-81 0.0916831687517039 1106 82-83 0.030110236009331715 1106 84-85 0.1393288808237685 1106 86-87 0.3722160459438193 1106 88-89 0.38561681963235017 1106 90-91 0.5014313993786708 1106 92-93 0.2338593237989386 1106 94-95 -0.3275188291098239 1106 96-97 0.05642403696723264 1106 98-99 0.26593120997880604 1106 100-101 0.1877839653995501 1107 1 0.24855324294227188 1107 2 0.13721530822398975 1107 3 0.2344564997495162 1107 4 0.10180641059539397 1107 5 0.12685006861151749 1107 6 0.2630293993709074 1107 7 0.08923515506563007 1107 8 0.08935945378497934 1107 9 0.28937892889940997 1107 10-11 0.1851450516803439 1107 12-13 0.19635788494637296 1107 14-15 0.09297926263755585 1107 16-17 0.006087112330362743 1107 18-19 0.21177681666440407 1107 20-21 0.1891082427417814 1107 22-23 0.26883746637264494 1107 24-25 0.22352304681914603 1107 26-27 0.01753937372629366 1107 28-29 0.257287078769842 1107 30-31 0.29390302927236434 1107 32-33 0.3464697880381493 1107 34-35 0.26480435993723006 1107 36-37 0.3172566846056242 1107 38-39 0.30568056414333 1107 40-41 0.25295768909303007 1107 42-43 0.40494683795667186 1107 44-45 0.5733892625748211 1107 46-47 0.45030113275009853 1107 48-49 0.3746321067402931 1107 50-51 0.4429942887777969 1107 52-53 0.5681978920481896 1107 54-55 0.658608989711837 1107 56-57 0.7583709269544592 1107 58-59 0.6164527774375976 1107 60-61 0.73686468596512 1107 62-63 0.8288449069786843 1107 64-65 0.7488234024652449 1107 66-67 0.7887326696914911 1107 68-69 0.7594615029114671 1107 70-71 0.5135533732880511 1107 72-73 0.45102748565052764 1107 74-75 0.4587620998122297 1107 76-77 0.6889265877368196 1107 78-79 0.5775352006426004 1107 80-81 0.9029661022224111 1107 82-83 1.213231985592298 1107 84-85 1.2200109777720556 1107 86-87 1.2299017490151591 1107 88-89 0.9676548924416899 1107 90-91 1.044557073993527 1107 92-93 1.4754947128677216 1107 94-95 1.051279559173956 1107 96-97 0.9427735119942184 1107 98-99 1.4628098896961994 1107 100-101 1.7765911850050848 1108 1 -0.24175840686304895 1108 2 -0.053997242482232366 1108 3 -0.06754731238087786 1108 4 -0.10125632664455964 1108 5 -0.19510389258294936 1108 6 -0.029427795617934294 1108 7 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78-79 -0.05688312364913983 2316 80-81 0.32583530053464926 2316 82-83 0.08404572881110539 2316 84-85 -0.010392879973576186 2316 86-87 -0.5135702220517508 2316 88-89 -0.21738428778736107 2316 90-91 -0.034201214131847735 2316 92-93 -0.02900197188212772 2316 94-95 -0.24199349688390726 2316 96-97 -0.5511867050039712 2316 98-99 -0.40556865159373245 2316 100-101 -0.4634570368152886 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 17.0 4 31.0 5 82.0 6 222.0 7 478.0 8 1079.0 9 1841.0 10 2740.0 11 4193.0 12 6059.0 13 8242.0 14 10743.0 15 14176.0 16 18248.0 17 22427.0 18 19585.0 19 15636.0 20 15568.0 21 16358.0 22 17881.0 23 20187.0 24 23294.0 25 27231.0 26 32667.0 27 39058.0 28 46210.0 29 54236.0 30 63867.0 31 73636.0 32 86293.0 33 100821.0 34 116888.0 35 131936.0 36 136618.0 37 104650.0 38 32798.0 39 1091.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.524479582386924 23.90427489702967 16.234850512423943 19.336395008159464 2 19.178954645755795 24.614550482522183 35.66323518734236 20.543259684379667 3 22.01592307425191 26.25610452911075 30.75996060270288 20.96801179393446 4 16.753558541921187 20.32346506804557 35.32498034870396 27.59799604132928 5 13.53462034518523 39.035877731243005 34.03656443925949 13.392937484312274 6 31.006193709691164 38.95518241769343 16.257461968033894 13.781161904581515 7 27.24790307254722 34.61674448264214 20.735826601383327 17.399525843427313 8 26.476925116723965 36.52434077399273 19.121658095860443 17.87707601342286 9 26.25413150742014 16.820167754196223 20.350140321302636 36.575560417081 10-11 24.99719830919933 28.131580027338188 26.97980888522696 19.89141277823552 12-13 25.720436047945544 25.36658609973857 28.05919202880679 20.8537858235091 14-15 22.69775991009335 26.686894085038816 26.50719126945794 24.108154735409897 16-17 21.56070750979408 30.112967329917637 27.117880943135937 21.208444217152344 18-19 21.502976895126793 27.945997607119295 30.38934820833846 20.16167728941545 20-21 22.17096311870014 27.003485145514293 30.808812619762417 20.016739116023146 22-23 21.916570875216195 27.055096558965904 30.700341060970715 20.327991504847184 24-25 21.92729008989101 27.261796715308307 30.6971880450481 20.113725149752582 26-27 21.67571641661471 27.44970680388613 30.593692534745514 20.280884244753643 28-29 21.53776822272944 27.393957905171405 30.73934291153267 20.32893096056648 30-31 21.616465102770754 27.297307535680126 30.736481644584607 20.349745716964517 32-33 21.577359812862838 27.377254374583693 30.643868006427315 20.401517806126154 34-35 21.98668289279705 27.30006976604698 30.436740189346946 20.276507151809025 36-37 22.013358146054117 27.083629286586923 30.45106432682078 20.451948240538176 38-39 21.738003238912405 27.47223563876972 30.353794357473646 20.435966764844228 40-41 21.89852828366054 27.335426314742733 30.33970698260268 20.426338418994042 42-43 21.91313881403532 27.4942419319956 29.959042337388848 20.63357691658024 44-45 22.02057940544175 27.61733165389727 29.637548023347946 20.72454091731303 46-47 22.13690876432019 27.93707954907773 29.09421731018742 20.831794376414656 48-49 22.364319244380045 28.131540566904377 28.759513910592126 20.74462627812345 50-51 22.312507694784596 28.232361975294612 28.530840696650284 20.92428963327051 52-53 22.66587587957307 28.432781518626904 28.081386355529038 20.81995624627099 54-55 22.368423129496517 28.419167668961688 28.322726368724606 20.88968283281719 56-57 22.344036581400562 28.60688095260644 28.189034418968788 20.86004804702421 58-59 22.47591335120102 28.243647659364907 28.35366334883339 20.926775640600685 60-61 22.445844500636102 28.30457456917098 28.50471788946659 20.744863040726326 62-63 22.332080069955456 28.331486585030923 28.582691706679547 20.753741638334073 64-65 22.283070211160673 28.194677261003935 28.46458662827956 21.057665899555833 66-67 22.249962907192216 28.618442859713422 28.174947044097827 20.956647188996538 68-69 22.280071218190944 28.346086945541444 28.26677147357887 21.10707036268874 70-71 22.282241542050617 28.491340802404245 28.178459022707113 21.047958632838025 72-73 22.49277675048872 28.513190470440513 27.918678167042728 21.07535461202804 74-75 22.399952016112483 28.719382649405095 27.597206832653036 21.283458501829386 76-77 22.28922603883538 28.60277706748997 27.950140952669578 21.157855941005074 78-79 22.374065971531664 28.49106457936756 27.90270951122728 21.232159937873494 80-81 22.24309679170889 28.68994516578117 27.807688786607443 21.259269255902492 82-83 22.383492885272556 28.57847508858881 27.839735141150197 21.198296884988437 84-85 22.308917718988997 28.536116744148053 27.77310166655026 21.381863870312692 86-87 22.388113885968817 28.399476912489384 27.82572220485963 21.386686996682165 88-89 22.350034567340018 28.345573959901888 27.757337273063044 21.54705419969505 90-91 22.282413948238712 28.52648444714555 27.789213635681982 21.401887968933757 92-93 22.3364744136124 28.36194241878966 27.680888341699024 21.620694825898916 94-95 22.2418340578269 28.247041256672762 27.651543850012473 21.859580835487876 96-97 22.416091333541686 28.30863899385364 27.48040394856885 21.794865724035827 98-99 22.24127725733768 28.234497944108156 27.447813493911244 22.076411304642924 100-101 22.42739221307335 28.07239309709032 27.660575368761364 21.839639321074962 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 405.0 1 282.5 2 177.0 3 231.0 4 236.0 5 232.0 6 415.0 7 827.0 8 1271.5 9 1528.5 10 1537.5 11 1487.5 12 1601.0 13 1764.5 14 1949.0 15 2194.0 16 2397.0 17 2577.5 18 2749.0 19 2938.0 20 3227.5 21 3666.0 22 4243.5 23 4990.0 24 5807.5 25 7021.5 26 8957.5 27 11146.5 28 13097.0 29 15483.5 30 18136.0 31 20940.5 32 23944.0 33 27029.5 34 30217.5 35 32700.5 36 35797.0 37 38707.0 38 40110.5 39 40671.0 40 41171.5 41 42469.0 42 44402.0 43 45593.0 44 46764.0 45 48212.0 46 47622.0 47 46218.5 48 46348.5 49 45864.5 50 44235.5 51 41983.0 52 38807.0 53 36484.5 54 34794.0 55 31991.0 56 28336.0 57 24952.0 58 22152.0 59 19680.5 60 16778.0 61 13774.0 62 11203.0 63 8747.0 64 6525.5 65 5058.5 66 3920.0 67 3017.0 68 2167.0 69 1431.0 70 990.5 71 741.0 72 603.5 73 470.0 74 354.0 75 243.5 76 173.5 77 125.5 78 90.0 79 53.0 80 22.0 81 11.5 82 10.5 83 6.0 84 1.5 85 1.0 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0366982034453694 2 0.0 3 0.0 4 0.0 5 0.014047914437152156 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002328165594921284 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0013021943158034303 24-25 1.5784173524890064E-4 26-27 0.001736259087737907 28-29 0.003788201645973615 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.013732230966654355 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.3676260287335094E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.5784173524890064E-4 84-85 0.0010654317129300793 86-87 0.0 88-89 0.0 90-91 0.009628345850182938 92-93 9.470504114934038E-4 94-95 0.0 96-97 0.0 98-99 1.5784173524890064E-4 100-101 0.011680288408418647 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1267092.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.1596316083107 #Duplication Level Percentage of deduplicated Percentage of total 1 80.61693076345372 49.30501786885519 2 11.251513277222287 13.762768141418633 3 3.510354920841816 6.440760413193184 4 1.4826975343863018 3.627249399584672 5 0.8044835291762266 2.460095813968584 6 0.5103726719051941 1.8728522758002533 7 0.343105821262393 1.4688957941752394 8 0.2324117001311695 1.13713711691868 9 0.16903469871826898 0.9304289912368008 >10 0.9777327791469398 11.14957023521806 >50 0.06701923521486934 2.795416896688787 >100 0.0330519847475101 3.384809910355413 >500 6.455418966306365E-4 0.216277490920245 >1k 3.8732513797838197E-4 0.5098589846192121 >5k 2.582167586522546E-4 0.9388606670469927 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6396 0.5047778693259842 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5494 0.43359124672873006 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3942 0.3111060601755832 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1440 0.11364604937920847 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.892086762445032E-5 0.0 0.0 0.0 0.0 3 7.892086762445032E-5 0.0 0.0 0.0 0.0 4 7.892086762445032E-5 0.0 0.0 0.0 0.0 5 7.892086762445032E-5 0.0 0.0 0.0 0.0 6 7.892086762445032E-5 0.0 0.0 0.0 0.0 7 7.892086762445032E-5 0.0 0.0 0.0 0.0 8 7.892086762445032E-5 0.0 0.0 0.0 0.0 9 7.892086762445032E-5 0.0 0.0 0.0 0.0 10-11 7.892086762445032E-5 0.0 0.0 0.0 0.0 12-13 7.892086762445032E-5 0.0 0.0 0.0 0.0 14-15 7.892086762445032E-5 0.0 0.0 0.0 0.0 16-17 7.892086762445032E-5 0.0 0.0 0.0 7.892086762445032E-5 18-19 7.892086762445032E-5 0.0 0.0 0.0 7.892086762445032E-5 20-21 7.892086762445032E-5 0.0 0.0 1.1838130143667547E-4 7.892086762445032E-5 22-23 7.892086762445032E-5 0.0 0.0 3.9460433812225156E-4 1.1838130143667548E-4 24-25 7.892086762445032E-5 0.0 0.0 7.49748242432278E-4 1.5784173524890064E-4 26-27 7.892086762445032E-5 0.0 0.0 0.0013416547496156554 1.5784173524890064E-4 28-29 7.892086762445032E-5 0.0 0.0 0.002564928197794635 1.5784173524890064E-4 30-31 7.892086762445032E-5 0.0 0.0 0.005761223336584873 1.5784173524890064E-4 32-33 7.892086762445032E-5 0.0 0.0 0.009825648019244064 1.5784173524890064E-4 34-35 7.892086762445032E-5 0.0 0.0 0.014600360510523307 1.5784173524890064E-4 36-37 7.892086762445032E-5 0.0 0.0 0.02071672775141821 1.5784173524890064E-4 38-39 7.892086762445032E-5 0.0 0.0 0.03125266357928232 1.5784173524890064E-4 40-41 7.892086762445032E-5 0.0 0.0 0.04881255662572252 1.5784173524890064E-4 42-43 7.892086762445032E-5 0.0 0.0 0.06629352880453826 1.5784173524890064E-4 44-45 7.892086762445032E-5 0.0 0.0 0.08882543651131883 1.5784173524890064E-4 46-47 7.892086762445032E-5 0.0 0.0 0.1235900786998892 1.5784173524890064E-4 48-49 7.892086762445032E-5 0.0 0.0 0.15448759837486148 1.5784173524890064E-4 50-51 7.892086762445032E-5 0.0 0.0 0.1877527440785673 1.5784173524890064E-4 52-53 7.892086762445032E-5 0.0 0.0 0.21900540765784965 1.5784173524890064E-4 54-55 7.892086762445032E-5 0.0 0.0 0.2525862368320533 1.5784173524890064E-4 56-57 7.892086762445032E-5 0.0 0.0 0.3120925710208888 1.9730216906112578E-4 58-59 7.892086762445032E-5 0.0 0.0 0.3682842287694974 2.3676260287335094E-4 60-61 7.892086762445032E-5 0.0 0.0 0.42052984313688346 2.3676260287335094E-4 62-63 7.892086762445032E-5 0.0 0.0 0.4764847382826188 2.3676260287335094E-4 64-65 7.892086762445032E-5 0.0 0.0 0.5421074397123493 2.3676260287335094E-4 66-67 7.892086762445032E-5 0.0 0.0 0.620752084300114 2.3676260287335094E-4 68-69 7.892086762445032E-5 0.0 0.0 0.712773815950223 2.3676260287335094E-4 70-71 7.892086762445032E-5 0.0 0.0 0.8217240737057767 2.3676260287335094E-4 72-73 7.892086762445032E-5 0.0 0.0 0.9317792236080726 2.3676260287335094E-4 74-75 7.892086762445032E-5 0.0 0.0 1.065234410761018 2.3676260287335094E-4 76-77 7.892086762445032E-5 0.0 0.0 1.2179462896143294 2.3676260287335094E-4 78-79 7.892086762445032E-5 0.0 0.0 1.3953209396002815 2.3676260287335094E-4 80-81 1.5784173524890064E-4 0.0 0.0 1.5902160221988617 2.3676260287335094E-4 82-83 1.5784173524890064E-4 0.0 0.0 1.7928058893908256 3.156834704978013E-4 84-85 1.5784173524890064E-4 0.0 0.0 2.0026564764042387 3.156834704978013E-4 86-87 1.5784173524890064E-4 0.0 0.0 2.2244241144289445 3.156834704978013E-4 88-89 1.5784173524890064E-4 0.0 0.0 2.4706966818510416 3.156834704978013E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3200 0.0 63.402016 1 GTATCAA 5815 0.0 41.100147 1 TGGTATC 1175 0.0 33.151123 2 GTGGTAT 1235 0.0 32.70221 1 TATCAAC 7860 0.0 30.278719 2 ATCAACG 7945 0.0 29.715618 3 TCAACGC 7985 0.0 29.566763 4 CAACGCA 8170 0.0 28.839113 5 AACGCAG 8470 0.0 27.814365 6 ATACCGT 155 1.4114703E-6 24.514847 6 ACGCAGA 9780 0.0 24.04205 7 TACCGTG 160 1.8593764E-6 23.750631 7 CGCAGAG 9895 0.0 23.474602 8 GCAGAGT 10285 0.0 22.214981 9 GAGTACT 6905 0.0 21.807241 12-13 AGTACTT 6975 0.0 21.486233 12-13 GTACTTT 7085 0.0 21.117456 14-15 AGAGTAC 9800 0.0 20.696978 10-11 CAGAGTA 10125 0.0 20.525236 10-11 ATACGTC 310 2.582965E-10 19.92067 5 >>END_MODULE