FastQCFastQC Report
Thu 26 May 2016
SRR937440_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937440_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356133
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69690.5138876496626806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48640.35866688591753165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35250.25993025757798094No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18230.13442634313891041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22750.059.113981
GTATCAA48300.049.6854741
ATCAACG64000.037.1917533
TATCAAC64750.036.907712
TCAACGC64600.036.847684
CAACGCA66400.035.7745975
AACGCAG68550.034.9259156
GCGTATC550.002574211534.5606961
ACGCAGA80300.029.460397
CGCAGAG80800.029.1594268
GTGGTAT8950.028.1409021
GCAGAGT85600.027.0248839
TGGTATC9450.026.6460572
GTACATG51650.025.9456141
TACATGG53000.024.920562
CCCTATA2900.024.5743562
CCTATAC4300.024.3076553
ACATGGG54600.023.581183
AGAGTAC81150.022.27197610-11
GAGTACT55000.022.1530412-13
Produced by FastQC (version 0.11.5)