##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937440_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1356133 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2355181976989 33.0 31.0 34.0 31.0 34.0 2 32.36747944338793 34.0 31.0 34.0 31.0 34.0 3 32.421496269171236 34.0 31.0 34.0 31.0 34.0 4 35.99418419874747 37.0 35.0 37.0 35.0 37.0 5 36.002284436703476 37.0 35.0 37.0 35.0 37.0 6 36.05034683176355 37.0 37.0 37.0 35.0 37.0 7 35.9630323869414 37.0 37.0 37.0 35.0 37.0 8 35.97771088823884 37.0 37.0 37.0 35.0 37.0 9 37.69196974043106 39.0 38.0 39.0 35.0 39.0 10-11 37.60600361468971 39.0 38.0 39.0 35.0 39.0 12-13 37.48620231201512 39.0 37.5 39.0 35.0 39.0 14-15 38.788261918263174 40.0 38.0 41.0 36.0 41.0 16-17 38.72685311838883 40.0 38.0 41.0 36.0 41.0 18-19 38.694819387184 40.0 38.0 41.0 35.0 41.0 20-21 38.58312975202285 40.0 38.0 41.0 35.0 41.0 22-23 38.440172166004366 40.0 38.0 41.0 34.5 41.0 24-25 38.393639856857696 40.0 38.0 41.0 34.0 41.0 26-27 38.42379914064476 40.0 38.0 41.0 34.5 41.0 28-29 38.33997955952698 40.0 38.0 41.0 34.0 41.0 30-31 38.130589698797976 40.0 38.0 41.0 34.0 41.0 32-33 37.89920900088708 40.0 38.0 41.0 34.0 41.0 34-35 37.9512510941036 40.0 38.0 41.0 34.0 41.0 36-37 37.84725539456676 40.0 38.0 41.0 34.0 41.0 38-39 37.74763574074224 40.0 38.0 41.0 33.0 41.0 40-41 37.53926458540571 40.0 37.5 41.0 33.0 41.0 42-43 37.27704436069324 40.0 37.0 41.0 33.0 41.0 44-45 37.040534741061535 40.0 37.0 41.0 32.0 41.0 46-47 36.68535128929095 40.0 37.0 41.0 31.0 41.0 48-49 36.35508206053536 39.5 36.0 41.0 30.0 41.0 50-51 35.409234566226175 38.5 35.0 40.0 29.0 40.5 52-53 35.539174255032506 38.5 35.5 40.0 29.5 40.5 54-55 35.91764745788208 39.0 36.0 40.0 29.5 41.0 56-57 35.72036776628841 39.0 35.0 40.0 28.5 41.0 58-59 35.474253631465345 39.0 35.0 40.0 28.0 41.0 60-61 35.18224539923444 38.0 35.0 40.0 28.0 41.0 62-63 34.878487581970205 38.0 35.0 40.0 27.0 41.0 64-65 34.47857584764916 37.5 34.0 40.0 26.0 41.0 66-67 34.129307007498525 37.0 34.0 40.0 26.0 41.0 68-69 33.66824603486531 36.5 34.0 39.0 25.5 41.0 70-71 33.21076546326946 36.0 33.0 39.0 24.0 40.0 72-73 32.75159516065165 35.0 33.0 38.5 22.5 40.0 74-75 32.26932019204606 35.0 32.0 37.5 21.5 39.5 76-77 31.796207304150848 35.0 32.0 37.0 20.0 39.0 78-79 31.300275857898892 35.0 31.0 36.5 18.0 39.0 80-81 30.826140208961803 34.5 31.0 36.0 15.5 37.5 82-83 30.363912684080397 34.0 30.5 35.5 9.5 37.0 84-85 29.858022406356902 34.0 30.0 35.0 7.0 37.0 86-87 29.403739529972356 34.0 29.5 35.0 2.0 36.0 88-89 28.960971748346218 34.0 29.0 35.0 2.0 36.0 90-91 28.47582722343605 34.0 29.0 35.0 2.0 35.0 92-93 27.987473573757146 33.5 27.5 35.0 2.0 35.0 94-95 27.453226564061193 33.0 26.5 35.0 2.0 35.0 96-97 26.740990743533267 33.0 25.5 35.0 2.0 35.0 98-99 25.833692565552198 32.0 24.5 34.0 2.0 35.0 100-101 23.182906838783513 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.08382765283212734 1101 2 -0.06506883066115421 1101 3 -0.11914569971060729 1101 4 -0.09638749773014865 1101 5 -0.15895504698047347 1101 6 -0.09154749374691562 1101 7 -0.1227948078365273 1101 8 -0.2540765193611776 1101 9 -0.18716252478996154 1101 10-11 -0.24358804560510805 1101 12-13 -0.2165889702316406 1101 14-15 -0.12209321673234541 1101 16-17 -0.25647068481247004 1101 18-19 -0.16135324166933174 1101 20-21 -0.020857562436368937 1101 22-23 -0.1682815243651774 1101 24-25 -0.05297018971572243 1101 26-27 -0.19206749981481863 1101 28-29 -0.3028791349596318 1101 30-31 -0.15358355801336643 1101 32-33 -0.34808165573842587 1101 34-35 -0.013487697099840545 1101 36-37 0.10321494285560107 1101 38-39 -0.05633656895872008 1101 40-41 -0.1266913010727535 1101 42-43 0.022397347129334833 1101 44-45 0.026266490527028452 1101 46-47 -0.009068504012539336 1101 48-49 -0.06051428997052 1101 50-51 0.08115438227672911 1101 52-53 -0.04779189411431872 1101 54-55 -0.0011752650086904737 1101 56-57 0.18075001511515865 1101 58-59 0.040740876041404306 1101 60-61 0.08073981084884707 1101 62-63 -0.007470884168192526 1101 64-65 -0.0845889426249471 1101 66-67 0.026588569035276066 1101 68-69 0.08216992084218333 1101 70-71 -0.08508686188173442 1101 72-73 -0.030866596776533584 1101 74-75 -0.06242198189214321 1101 76-77 -0.1277997014495753 1101 78-79 0.026567907171710203 1101 80-81 -0.05400813849336572 1101 82-83 -0.05556831137826279 1101 84-85 -0.049127766031546116 1101 86-87 0.01079891661379051 1101 88-89 -0.05979411128134515 1101 90-91 0.04412900453648305 1101 92-93 0.03290692329864697 1101 94-95 0.17067532888692227 1101 96-97 0.312128373344045 1101 98-99 0.5526723819028838 1101 100-101 0.8087773184756486 1102 1 0.07735426805415102 1102 2 0.10064061834975746 1102 3 0.12787546690996976 1102 4 0.0646145449187685 1102 5 0.11294299547505204 1102 6 0.022181766043466666 1102 7 0.09954855767818316 1102 8 0.09447702498663091 1102 9 0.12110824812478427 1102 10-11 0.11505876525328063 1102 12-13 0.1525186844049884 1102 14-15 0.2805041396974488 1102 16-17 0.3549628167627077 1102 18-19 0.1676522307527719 1102 20-21 0.19673804853464105 1102 22-23 0.16267879079855874 1102 24-25 0.2211773971786215 1102 26-27 0.16196646517789048 1102 28-29 0.08665447560895956 1102 30-31 0.15655034107138732 1102 32-33 0.09312037880530966 1102 34-35 0.2573843995196583 1102 36-37 0.28063300760750565 1102 38-39 0.43454651942975175 1102 40-41 0.3332529082344138 1102 42-43 0.3650995164039941 1102 44-45 0.6453320195290146 1102 46-47 0.6221718666361724 1102 48-49 0.5548758218454921 1102 50-51 0.5833978833755467 1102 52-53 0.47152137627822555 1102 54-55 0.5360405113791913 1102 56-57 0.6903032914230636 1102 58-59 0.516553829691837 1102 60-61 0.7480180398231795 1102 62-63 0.7330156544697317 1102 64-65 0.5733526815546668 1102 66-67 0.4650259532786478 1102 68-69 0.5304933205835312 1102 70-71 0.40223598827115836 1102 72-73 0.5901660393923578 1102 74-75 0.6163544123952676 1102 76-77 0.4843534908203537 1102 78-79 0.384580167331098 1102 80-81 0.4805727416586727 1102 82-83 0.3950300503789137 1102 84-85 0.6499677592662287 1102 86-87 0.4643679354668784 1102 88-89 0.3786159001867837 1102 90-91 0.4150091352432632 1102 92-93 0.49873610153949244 1102 94-95 0.5999071066171453 1102 96-97 0.7142490168957671 1102 98-99 1.0453768263173124 1102 100-101 1.3713122009895642 1103 1 0.07197509157654736 1103 2 -0.04426168111329076 1103 3 0.032783584235062335 1103 4 0.011253087450484145 1103 5 -0.05784979546999125 1103 6 -0.02228868451241084 1103 7 0.018921233968448803 1103 8 -5.555396248126954E-4 1103 9 0.06811007190388807 1103 10-11 0.05013765827767713 1103 12-13 0.08975749222586415 1103 14-15 0.0015398330853955144 1103 16-17 -0.022193830513323576 1103 18-19 -0.027827766324890035 1103 20-21 -0.08431244420744122 1103 22-23 -0.0904654016949209 1103 24-25 0.017520388296993872 1103 26-27 0.04901783462084097 1103 28-29 -0.08435430681573308 1103 30-31 -0.16023928490309203 1103 32-33 -0.08914124133521995 1103 34-35 -0.028169406655720763 1103 36-37 0.037534178351691594 1103 38-39 -0.0298413617223261 1103 40-41 -0.0778725464536194 1103 42-43 -0.20919049329467754 1103 44-45 -0.06420970989080388 1103 46-47 0.06700087310216674 1103 48-49 0.06603046638283416 1103 50-51 -0.004811595852068251 1103 52-53 0.12728072457042572 1103 54-55 0.21828487230916238 1103 56-57 0.10006359282585464 1103 58-59 0.1116688363575733 1103 60-61 0.32930205135969715 1103 62-63 0.1865521840077733 1103 64-65 0.13721712268802833 1103 66-67 0.16958161406512318 1103 68-69 0.11508841950393389 1103 70-71 0.17133121018439113 1103 72-73 0.20063286249784795 1103 74-75 0.19781352951459752 1103 76-77 0.15316432115929857 1103 78-79 0.005256567522256006 1103 80-81 0.034001392752504245 1103 82-83 0.11147345323837854 1103 84-85 0.17946207445091744 1103 86-87 0.36585487510387793 1103 88-89 0.22592718328676753 1103 90-91 0.20673118219233544 1103 92-93 0.19775725295337665 1103 94-95 0.020869976190464712 1103 96-97 0.15900211634836836 1103 98-99 0.4727090148455311 1103 100-101 0.7161730113212315 1104 1 0.10946092306572552 1104 2 0.05624028898440514 1104 3 0.059729243123292974 1104 4 -0.007122496532048217 1104 5 0.1403624233337979 1104 6 0.10220229861731411 1104 7 0.08996169743618054 1104 8 0.09417407421204871 1104 9 0.20039148701576437 1104 10-11 0.1858255132401041 1104 12-13 0.2807310263472331 1104 14-15 0.23350609253856902 1104 16-17 0.1572259952065025 1104 18-19 0.25966706104417625 1104 20-21 0.2526687184200753 1104 22-23 0.08695284075003684 1104 24-25 0.12761496498087865 1104 26-27 0.14096772157261483 1104 28-29 0.14777087984877113 1104 30-31 0.13941047550664365 1104 32-33 0.1467847045815489 1104 34-35 0.17019874073277208 1104 36-37 0.16493083068492354 1104 38-39 0.12620866736435943 1104 40-41 0.13812452688679144 1104 42-43 0.06203828392535371 1104 44-45 0.12576378224980544 1104 46-47 0.039286066483605 1104 48-49 0.15074016933888146 1104 50-51 -0.009029164205763607 1104 52-53 0.07447341032035837 1104 54-55 0.02553765053674084 1104 56-57 -0.008698094321438532 1104 58-59 0.17523672226582931 1104 60-61 0.16237300486299233 1104 62-63 0.06452907059371427 1104 64-65 0.13905827943823112 1104 66-67 0.19834893863047398 1104 68-69 0.022939123881336343 1104 70-71 0.05173998296645976 1104 72-73 0.17987486965856192 1104 74-75 0.1613802159722013 1104 76-77 0.15915557716404294 1104 78-79 0.144119968084798 1104 80-81 0.2620274568151615 1104 82-83 0.25149282436640163 1104 84-85 0.31001478131117466 1104 86-87 0.359427318924606 1104 88-89 0.23164256564164631 1104 90-91 0.3501060165182146 1104 92-93 0.4385409740091326 1104 94-95 0.6652658428835174 1104 96-97 0.47861373422737685 1104 98-99 0.6165876184450845 1104 100-101 0.9383022761179518 1105 1 0.0063203988358964125 1105 2 -0.013430675814440463 1105 3 -0.04593282408158217 1105 4 0.019827671082552456 1105 5 -0.0647844539287945 1105 6 0.009485895823317492 1105 7 -0.005387154372421321 1105 8 -0.05951867839215197 1105 9 -0.0919495092971303 1105 10-11 -0.07703174022414316 1105 12-13 0.032927698329011434 1105 14-15 -0.011050094972155478 1105 16-17 -0.145828943041856 1105 18-19 -0.013619545272867128 1105 20-21 -0.0570651698619713 1105 22-23 -0.02031945659532397 1105 24-25 -0.05435098058728016 1105 26-27 0.07590354254934795 1105 28-29 -0.09700398089091067 1105 30-31 0.058588745456127356 1105 32-33 0.02802997014919839 1105 34-35 0.1165725317523183 1105 36-37 0.16349114473734971 1105 38-39 0.20926735130725405 1105 40-41 0.24961700829433653 1105 42-43 0.3623388811439199 1105 44-45 0.34953764274256116 1105 46-47 0.294306657340762 1105 48-49 0.3888732231052927 1105 50-51 0.29644739695368827 1105 52-53 0.18342446936840417 1105 54-55 0.4011622817218452 1105 56-57 0.3624207174354481 1105 58-59 0.4093708809074954 1105 60-61 0.5430825733338764 1105 62-63 0.581622725512716 1105 64-65 0.4634087467083958 1105 66-67 0.3799617635537871 1105 68-69 0.24271827438292348 1105 70-71 0.36774006248399616 1105 72-73 0.47586216359304956 1105 74-75 0.5864459225692258 1105 76-77 0.5354100709512153 1105 78-79 0.48736160514164695 1105 80-81 0.6073025456446217 1105 82-83 0.5179832969098328 1105 84-85 0.6354920957946675 1105 86-87 0.6467785897404177 1105 88-89 0.6491514904389817 1105 90-91 0.7804052326426962 1105 92-93 0.9095270124035508 1105 94-95 0.7785390986244778 1105 96-97 0.8937957247606043 1105 98-99 0.8703249427217905 1105 100-101 0.8402634297081697 1106 1 -0.08851808869682287 1106 2 -0.0014075116266027976 1106 3 -0.006536640901060764 1106 4 0.041276331722016835 1106 5 0.06700338136840855 1106 6 -0.06040812542727991 1106 7 -0.2692713456217106 1106 8 -0.15137403781490377 1106 9 -0.11579548969197617 1106 10-11 -0.5059695302716491 1106 12-13 -0.25415838883682795 1106 14-15 -0.2837206497608591 1106 16-17 -0.13167660544560533 1106 18-19 -0.31525257176145516 1106 20-21 -0.18013939155196823 1106 22-23 -0.2581698370279568 1106 24-25 -0.2024207077788418 1106 26-27 -0.2859005338845506 1106 28-29 -0.1330391933816557 1106 30-31 -0.11552317212542818 1106 32-33 -0.15218532394334972 1106 34-35 0.06457880272029115 1106 36-37 -0.2919257161830586 1106 38-39 -0.106150835109311 1106 40-41 -0.0012472304290440661 1106 42-43 0.031389536426551956 1106 44-45 -0.05821925212273982 1106 46-47 -0.011729454049671517 1106 48-49 0.1229025180937029 1106 50-51 0.2207284295608929 1106 52-53 0.2708184677656149 1106 54-55 0.06668513662724251 1106 56-57 0.15337550125769184 1106 58-59 0.2627580568084582 1106 60-61 0.12176105367539236 1106 62-63 0.13103592684491616 1106 64-65 0.19482856637998225 1106 66-67 0.40857115050419424 1106 68-69 0.270999334414185 1106 70-71 0.49803366749833344 1106 72-73 0.28277851107415586 1106 74-75 0.5400257524791243 1106 76-77 0.4396936326660139 1106 78-79 0.2853041913522212 1106 80-81 0.08900422369227456 1106 82-83 0.1823531987012359 1106 84-85 0.028696337991760856 1106 86-87 0.3675155960692784 1106 88-89 0.665297404711378 1106 90-91 0.6805264317444077 1106 92-93 0.806022141586098 1106 94-95 0.9472720918994852 1106 96-97 1.1195985640647663 1106 98-99 0.8563341023572342 1106 100-101 1.4460547813790576 1107 1 0.06383390426324809 1107 2 0.05396556632615557 1107 3 0.00882922242379891 1107 4 0.07184589100693017 1107 5 0.03207147952940659 1107 6 0.04454772541757279 1107 7 0.10694602173468581 1107 8 0.0895035424053674 1107 9 0.13220127828413553 1107 10-11 0.03775580593243433 1107 12-13 0.11032974868109591 1107 14-15 0.13823620482314425 1107 16-17 0.19019669857089383 1107 18-19 0.1745941740126895 1107 20-21 0.14613426931361317 1107 22-23 0.2131473663816834 1107 24-25 0.08591751753593257 1107 26-27 0.12997180925997753 1107 28-29 0.2164345108364003 1107 30-31 0.252982752847295 1107 32-33 0.21289385498022995 1107 34-35 0.2821683809799964 1107 36-37 0.2484307042291647 1107 38-39 0.2778006214448041 1107 40-41 0.3901430979254883 1107 42-43 0.266359331238327 1107 44-45 0.29256412543927723 1107 46-47 0.2980684473994799 1107 48-49 0.5223403513759379 1107 50-51 0.4870725543707195 1107 52-53 0.3570409549196185 1107 54-55 0.44447524433710583 1107 56-57 0.5678657821347954 1107 58-59 0.5509221847403083 1107 60-61 0.6261775868202264 1107 62-63 0.7259325481829038 1107 64-65 0.6261335000693009 1107 66-67 0.523247135256419 1107 68-69 0.5115258890168661 1107 70-71 0.7632393854953534 1107 72-73 0.7663017406392498 1107 74-75 0.6545395698665359 1107 76-77 0.6164412994375539 1107 78-79 0.568122457948899 1107 80-81 0.7687172076769357 1107 82-83 0.7668434067850143 1107 84-85 0.7385307978692879 1107 86-87 0.7874884279805805 1107 88-89 0.9545107676257594 1107 90-91 0.8800280805133518 1107 92-93 0.8199078738506103 1107 94-95 1.0434761623407844 1107 96-97 1.3188240025839235 1107 98-99 1.2493382184156836 1107 100-101 1.4025967584620993 1108 1 0.01208459481856039 1108 2 -0.013951622098041128 1108 3 -0.016866692975980868 1108 4 -0.0246499840148644 1108 5 -0.016907683261450757 1108 6 0.0707650507431623 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0.09728977989763621 2316 96-97 0.28950983529036023 2316 98-99 0.5555460839905919 2316 100-101 0.8224174791958525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5416.0 3 1059.0 4 643.0 5 736.0 6 732.0 7 855.0 8 1064.0 9 1249.0 10 1543.0 11 2158.0 12 3223.0 13 4467.0 14 5100.0 15 6868.0 16 9324.0 17 13777.0 18 11390.0 19 6505.0 20 6160.0 21 6228.0 22 6709.0 23 7261.0 24 8157.0 25 9448.0 26 10734.0 27 12808.0 28 15560.0 29 19612.0 30 24548.0 31 32282.0 32 43332.0 33 60534.0 34 89466.0 35 138193.0 36 219498.0 37 314752.0 38 232353.0 39 22387.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.79859416929832 19.052250459877065 15.112643090314757 24.036512280509857 2 14.5061575599408 19.915367262780435 41.25627966191239 24.322195515366378 3 18.07828082447842 24.46172328343256 30.4121357738462 27.047860118242816 4 12.040097206244075 16.526908677408414 37.90356366247947 33.529430453868045 5 13.102601368550241 37.06065377422689 34.926330147044155 14.910414710178713 6 30.83439693328475 36.621809477285275 17.98073917178882 14.56305441764115 7 27.52240420842898 32.989060626977874 21.290908096555007 18.19762706803814 8 25.743447859996365 34.506016012154696 20.315513640777343 19.435022487071596 9 25.63448888415969 16.47816365106178 20.849793171328628 37.03755429344991 10-11 23.883080497724148 26.669790314081766 28.192457573945823 21.254671614248267 12-13 24.82118970371958 24.169173237948137 28.784356039050778 22.225281019281507 14-15 21.91070268688524 25.383014711696127 27.213460363251574 25.492822238167058 16-17 21.1596552767685 28.84006759076181 27.667132126045757 22.33314500642393 18-19 20.879939819532492 27.021872318980122 30.605174308319967 21.493013553167415 20-21 21.426386340645916 26.18613064524591 30.85517268897659 21.532310325131583 22-23 21.226367692328154 26.128099558277384 30.715756152564992 21.92977659682947 24-25 21.025246388451617 26.361615529464093 30.803867145115476 21.809270936968815 26-27 20.801678519480202 26.685142006546048 30.53754963200182 21.975629841971926 28-29 20.754977717722845 26.702078878632996 30.748397046863907 21.794546356780252 30-31 20.89072224677319 26.456258304426417 30.753905205018643 21.899114243781746 32-33 20.684943502710045 26.56527292767216 30.728347816833573 22.02143575278422 34-35 21.213200651829297 26.66768899908729 30.314016177486764 21.805094171596647 36-37 21.036898835308353 26.46796405249887 30.383544689615675 22.1115924225771 38-39 20.820200696129834 26.710444792308213 30.49896201211891 21.970392499443044 40-41 21.06196285723469 26.605521872883802 30.492844130650266 21.83967113923125 42-43 20.948206397066187 26.642779793597022 30.249012893073978 22.160000916262813 44-45 20.90565619428561 26.96871218349607 30.04262237536142 22.0830092468569 46-47 21.002170920747538 27.025062587183253 29.659692006392667 22.313074485676545 48-49 21.21723636011578 27.23427967938369 29.320776316873744 22.227707643626786 50-51 21.253699001084943 27.311192046519466 29.111545022127487 22.3235639302681 52-53 21.37586916237398 27.352808779473172 29.080711340152842 22.190610718000002 54-55 21.240727157165797 27.249880365315367 29.192821549393884 22.31657092812496 56-57 21.197165302666228 27.669960661222486 28.991289904032296 22.141584132078982 58-59 21.296857245446336 27.30058380851199 29.136787915414995 22.265771030626674 60-61 21.33590039296403 27.319089895601152 29.311651727039607 22.033357984395213 62-63 21.161127207401034 27.334664956022834 29.370407462505167 22.133800374070965 64-65 21.10710525829747 27.229743457141275 29.28094121123691 22.382210073324345 66-67 21.042337263396483 27.55638183990195 29.063160515461554 22.33812038124001 68-69 21.012153959761644 27.33054330242972 29.271830009320215 22.385472728488423 70-71 21.07537789428343 27.43838692207609 29.11531473889295 22.37092044474753 72-73 21.220105061729306 27.469837235590955 28.910704918396146 22.399352784283593 74-75 21.006672309122862 27.671592794452906 28.81971318047966 22.50202171594457 76-77 21.11327531845498 27.46981337159941 28.887722220210726 22.52918908973488 78-79 21.0965156307841 27.520398821623083 28.92513375701111 22.457951790581703 80-81 21.010221316550496 27.692645292091445 28.80665353475499 22.49047985660307 82-83 21.08556020733137 27.677466806995714 28.77777412465815 22.459198861014766 84-85 21.158343920478746 27.46430509597883 28.650385551880586 22.72696543166184 86-87 21.107465092926667 27.39018412432881 28.878311249103174 22.62403953364135 88-89 21.155747985245142 27.361470018341983 28.796208215655888 22.68657378075699 90-91 21.042891633923904 27.441557170064712 28.94460238555735 22.570948810454038 92-93 21.158165542148023 27.338695853910107 28.859308088554737 22.64383051538714 94-95 20.993083392717228 27.21123266184281 28.870583011293466 22.925100934146496 96-97 21.158570037886275 27.1208832618771 28.85297502387519 22.86757167636144 98-99 20.977384773626063 27.20750796550155 28.754389153912996 23.060718106959396 100-101 21.129954021230688 26.837497723594716 29.122509945074448 22.91003831010015 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1998.0 1 1183.5 2 408.5 3 427.5 4 336.0 5 306.0 6 521.5 7 1063.5 8 1789.5 9 2066.0 10 1830.5 11 1603.0 12 1612.5 13 1756.5 14 1927.0 15 2087.5 16 2132.5 17 2273.0 18 2463.5 19 2558.5 20 2816.5 21 3258.5 22 3787.5 23 4512.0 24 5518.5 25 6932.0 26 8787.0 27 11198.5 28 13376.0 29 15680.0 30 18400.5 31 21146.5 32 24325.5 33 27626.5 34 31615.5 35 34704.5 36 37551.5 37 40809.0 38 41950.0 39 42316.0 40 43227.0 41 44745.0 42 46381.0 43 48442.0 44 50549.5 45 51789.5 46 50887.0 47 49374.0 48 49952.0 49 49291.5 50 47144.5 51 45217.0 52 42895.0 53 41231.0 54 39550.0 55 36429.5 56 32427.0 57 28210.0 58 25057.0 59 22220.0 60 19090.5 61 15773.0 62 12301.0 63 9418.0 64 7242.5 65 5875.0 66 4469.0 67 3221.0 68 2277.5 69 1483.0 70 1129.5 71 847.0 72 639.5 73 490.0 74 356.0 75 290.0 76 195.0 77 105.0 78 71.5 79 52.0 80 34.5 81 22.0 82 15.5 83 12.5 84 8.0 85 6.5 86 7.0 87 4.0 88 4.0 89 4.5 90 2.5 91 3.0 92 3.5 93 2.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2251991508207528 2 0.2032249049318909 3 0.1951873451940186 4 0.20071777620631606 5 0.20130768884762779 6 0.22298697841583384 7 0.22409306461829334 8 0.21465446235730568 9 0.20934524858550008 10-11 0.22224958761419417 12-13 0.22291323933566987 14-15 0.21059881294828753 16-17 0.22044298015017702 18-19 0.21163116007058305 20-21 0.20676438077976128 22-23 0.20610072905828555 24-25 0.20141829746787374 26-27 0.2062850767586955 28-29 0.2158711571800111 30-31 0.21671915660189672 32-33 0.21579741809984715 34-35 0.22254454393485007 36-37 0.20429412159426844 38-39 0.2059901204380396 40-41 0.20156577562820166 42-43 0.20716994572066308 44-45 0.21904193762706164 46-47 0.22272889163525997 48-49 0.22501480312034292 50-51 0.2260102807025565 52-53 0.2396520105328902 54-55 0.22530975944099876 56-57 0.2035936003327107 58-59 0.19223778198745992 60-61 0.18980439234204904 62-63 0.19515047565393662 64-65 0.1886245670594256 66-67 0.18803465441811384 68-69 0.19345447681016537 70-71 0.19511360611385462 72-73 0.19489238887336272 74-75 0.1993536032232827 76-77 0.20661690261943333 78-79 0.20248751413025123 80-81 0.1981369084005772 82-83 0.19522421473410057 84-85 0.19787882162000336 86-87 0.20307742677156296 88-89 0.19765760437951146 90-91 0.20503151239590808 92-93 0.19651464863697 94-95 0.1955191710547564 96-97 0.20208194918934944 98-99 0.2062482072186135 100-101 0.18976752280196707 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1356133.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.05998839231523 #Duplication Level Percentage of deduplicated Percentage of total 1 78.24149759861963 45.42700442373216 2 12.434832883641606 14.439325057692228 3 3.951955905397881 6.883515419830277 4 1.7700119695401255 4.110674976230348 5 0.959629449314982 2.7858037344075854 6 0.5794877434296452 2.0187030994208484 7 0.36884684864248224 1.4990670625507199 8 0.257174842311402 1.1945254687516396 9 0.19809644674988477 1.035132965897148 >10 1.1147747636527103 11.840244452609822 >50 0.08499551147243188 3.391291420177837 >100 0.03755420140749428 3.8247354162646636 >500 5.074825865598537E-4 0.18835138849565292 >1k 5.074825865598537E-4 0.8482810285162061 >5k 1.2687064663996343E-4 0.5133440854228413 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6969 0.5138876496626806 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4864 0.35866688591753165 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3525 0.25993025757798094 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1823 0.13442634313891041 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.373908016396622E-5 0.0 14-15 0.0 0.0 0.0 1.1060862024594934E-4 1.4747816032793245E-4 16-17 0.0 0.0 0.0 1.4747816032793245E-4 1.4747816032793245E-4 18-19 0.0 0.0 0.0 2.949563206558649E-4 2.2121724049189865E-4 20-21 0.0 0.0 0.0 3.3182586073784797E-4 2.2121724049189865E-4 22-23 0.0 0.0 0.0 5.899126413117298E-4 2.5808678057388177E-4 24-25 0.0 0.0 0.0 9.217385020495778E-4 2.949563206558649E-4 26-27 0.0 0.0 0.0 0.0013273034429513919 2.949563206558649E-4 28-29 0.0 0.0 0.0 0.002691476425984767 2.949563206558649E-4 30-31 0.0 0.0 0.0 0.0054198223920515175 2.949563206558649E-4 32-33 0.0 0.0 0.0 0.00888555915975793 2.949563206558649E-4 34-35 0.0 0.0 0.0 0.011724513746070628 2.949563206558649E-4 36-37 0.0 0.0 0.0 0.01648068441664645 2.949563206558649E-4 38-39 0.0 0.0 0.0 0.02551372173673231 2.949563206558649E-4 40-41 0.0 0.0 0.0 0.03985597282862374 3.686954008198311E-4 42-43 0.0 0.0 0.0 0.053718919899449394 4.055649409018142E-4 44-45 0.0 0.0 0.0 0.07171125545945715 4.424344809837973E-4 46-47 0.0 0.0 0.0 0.10198114786676528 4.424344809837973E-4 48-49 0.0 0.0 0.0 0.1286009558059571 4.424344809837973E-4 50-51 0.0 0.0 0.0 0.15857589189260934 4.424344809837973E-4 52-53 0.0 0.0 0.0 0.18659674235491652 4.424344809837973E-4 54-55 0.0 0.0 0.0 0.21863637268615985 4.424344809837973E-4 56-57 0.0 0.0 0.0 0.2701431201806902 4.424344809837973E-4 58-59 0.0 0.0 0.0 0.32087560733349896 4.424344809837973E-4 60-61 0.0 0.0 0.0 0.3675893146173716 4.424344809837973E-4 62-63 0.0 0.0 0.0 0.42134510405690295 5.161735611477635E-4 64-65 0.0 0.0 0.0 0.4853506256392256 5.161735611477635E-4 66-67 0.0 0.0 0.0 0.5611912695878649 5.161735611477635E-4 68-69 0.0 0.0 0.0 0.6502312088858542 5.161735611477635E-4 70-71 0.0 0.0 0.0 0.7489678372254049 5.161735611477635E-4 72-73 0.0 0.0 0.0 0.8547834172606964 5.161735611477635E-4 74-75 0.0 0.0 0.0 0.9787388110163237 5.161735611477635E-4 76-77 0.0 0.0 0.0 1.1205390621716307 5.161735611477635E-4 78-79 0.0 0.0 0.0 1.2839079942748977 5.161735611477635E-4 80-81 0.0 0.0 0.0 1.4660803918199763 5.530431012297466E-4 82-83 0.0 0.0 0.0 1.65894495598883 5.899126413117298E-4 84-85 0.0 0.0 0.0 1.8782818499365475 5.899126413117298E-4 86-87 0.0 0.0 0.0 2.1013056978924634 5.899126413117298E-4 88-89 0.0 0.0 0.0 2.350138223905767 5.899126413117298E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2275 0.0 59.11398 1 GTATCAA 4830 0.0 49.685474 1 ATCAACG 6400 0.0 37.191753 3 TATCAAC 6475 0.0 36.90771 2 TCAACGC 6460 0.0 36.84768 4 CAACGCA 6640 0.0 35.774597 5 AACGCAG 6855 0.0 34.925915 6 GCGTATC 55 0.0025742115 34.560696 1 ACGCAGA 8030 0.0 29.46039 7 CGCAGAG 8080 0.0 29.159426 8 GTGGTAT 895 0.0 28.140902 1 GCAGAGT 8560 0.0 27.024883 9 TGGTATC 945 0.0 26.646057 2 GTACATG 5165 0.0 25.945614 1 TACATGG 5300 0.0 24.92056 2 CCCTATA 290 0.0 24.574356 2 CCTATAC 430 0.0 24.307655 3 ACATGGG 5460 0.0 23.58118 3 AGAGTAC 8115 0.0 22.271976 10-11 GAGTACT 5500 0.0 22.15304 12-13 >>END_MODULE