FastQCFastQC Report
Thu 26 May 2016
SRR937440_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937440_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356133
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73130.539253893239085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62760.46278646710905197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42620.31427595965882404No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16510.12174322135070823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33000.060.774081
GTATCAA60150.041.1644171
TGGTATC12550.033.30572
GTGGTAT14350.030.4688321
TATCAAC81750.030.0387882
ATCAACG83400.029.5014483
TCAACGC83900.029.3822484
CAACGCA86450.028.460625
AACGCAG91350.027.0899876
ACGCAGA106000.023.1684157
CGCAGAG106200.022.9458688
TACATCG1703.1505606E-622.352225
GTGTAGC5200.021.9345021
GAGTACT72300.021.48429512-13
GCAGAGT111500.021.429149
AGTACTT73200.021.1552512-13
GTACTTT75550.020.5585714-15
AGAGTAC105150.020.46447210-11
CAGAGTA108250.019.63707510-11
GCACCGT3254.8748916E-1018.999396
Produced by FastQC (version 0.11.5)