##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937440_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1356133 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.080764939721988 31.0 31.0 33.0 30.0 34.0 2 31.344250158354676 31.0 31.0 34.0 30.0 34.0 3 31.29617227808777 31.0 31.0 34.0 28.0 34.0 4 35.155290815871304 35.0 35.0 37.0 32.0 37.0 5 34.978695304959025 35.0 35.0 37.0 32.0 37.0 6 35.121686442258984 36.0 35.0 37.0 32.0 37.0 7 35.04765904229158 36.0 35.0 37.0 32.0 37.0 8 35.01909989654407 36.0 35.0 37.0 32.0 37.0 9 36.50376179917456 38.0 35.0 39.0 32.0 39.0 10-11 36.3540659360107 38.0 35.0 39.0 32.0 39.0 12-13 36.197624421793435 38.0 35.0 39.0 32.0 39.0 14-15 36.998511576666886 38.0 36.0 40.0 31.0 41.0 16-17 36.90683915220705 38.0 36.0 40.0 31.0 41.0 18-19 36.88028054770439 38.5 36.0 40.0 31.0 41.0 20-21 36.731860370627366 38.0 35.5 40.0 31.0 41.0 22-23 36.50017402422918 38.0 35.0 40.0 30.0 41.0 24-25 36.379591456000256 38.0 35.0 40.0 30.0 41.0 26-27 36.221019988452454 38.0 35.0 40.0 30.0 41.0 28-29 35.97896850825103 38.0 34.0 40.0 29.0 41.0 30-31 36.00124250350076 38.0 35.0 40.0 30.0 41.0 32-33 35.86792482743212 38.0 34.0 40.0 29.0 41.0 34-35 35.64533235309516 38.0 34.0 40.0 29.0 41.0 36-37 35.324138561630754 38.0 34.0 40.0 27.0 41.0 38-39 35.102450128416606 38.0 34.0 40.0 27.0 41.0 40-41 34.77320218592129 38.0 33.0 40.0 26.0 41.0 42-43 34.43003820421743 37.0 33.0 40.0 25.0 41.0 44-45 33.93772255376132 37.0 33.0 40.0 24.0 41.0 46-47 33.39550914253985 37.0 32.0 39.5 22.5 40.0 48-49 32.889934836774856 37.0 31.0 39.0 19.5 40.0 50-51 32.474226348005686 36.0 31.0 39.0 17.5 40.0 52-53 32.04833412357048 36.0 30.0 39.0 14.5 40.0 54-55 31.65166985833985 35.5 30.0 39.0 12.0 40.0 56-57 31.21291569484704 35.0 29.0 38.5 8.5 40.0 58-59 30.778075601729327 35.0 28.5 38.0 8.0 40.0 60-61 30.25195242649504 34.0 28.0 38.0 7.0 40.0 62-63 29.679408656820534 34.0 27.5 38.0 3.5 40.0 64-65 29.06520599380739 34.0 26.0 37.5 2.0 39.5 66-67 28.424755167819086 33.0 26.0 37.0 2.0 39.0 68-69 27.780723940793415 32.0 25.0 36.0 2.0 39.0 70-71 27.239834514756296 32.0 23.5 36.0 2.0 38.5 72-73 26.564075205013076 31.0 22.0 35.0 2.0 38.0 74-75 25.888575088136633 31.0 20.5 35.0 2.0 37.5 76-77 25.18213368452799 29.5 21.0 34.0 2.0 36.0 78-79 25.698572706364345 31.0 21.5 34.0 2.0 36.0 80-81 25.36654037620204 31.0 20.0 34.0 2.0 36.0 82-83 24.814508975152144 31.0 18.5 34.0 2.0 36.0 84-85 24.151438317628138 30.0 15.5 34.0 2.0 35.0 86-87 23.41937922017973 30.0 7.5 34.0 2.0 35.0 88-89 22.530245927206252 29.0 2.0 34.0 2.0 35.0 90-91 21.55755519554498 29.0 2.0 33.5 2.0 35.0 92-93 20.680123925898123 27.5 2.0 33.0 2.0 34.5 94-95 19.707539378512287 26.0 2.0 32.5 2.0 34.0 96-97 18.668136163635868 25.0 2.0 32.0 2.0 34.0 98-99 17.317944478896983 22.0 2.0 32.0 2.0 34.0 100-101 15.014526598792301 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.059414145351460945 1101 2 0.017280511315011182 1101 3 0.09847572300911267 1101 4 0.05162677123428949 1101 5 0.08909789472352259 1101 6 -0.03690332603419222 1101 7 -0.09125174320087126 1101 8 -0.1039805435217005 1101 9 -0.019252940773768046 1101 10-11 0.033829650216894436 1101 12-13 0.029036263652237437 1101 14-15 -0.24833555132145335 1101 16-17 -0.1087549541305961 1101 18-19 0.07690077808705098 1101 20-21 0.09124092491743596 1101 22-23 0.0023615723774241815 1101 24-25 0.12187914411466494 1101 26-27 -0.15405774748990098 1101 28-29 -0.16281361678530715 1101 30-31 -0.13802276914199751 1101 32-33 0.1473271732290513 1101 34-35 0.17235452493301295 1101 36-37 0.08803786181493933 1101 38-39 0.21708893884269287 1101 40-41 0.07176975393053908 1101 42-43 0.24438376173770138 1101 44-45 0.24304435297916172 1101 46-47 0.08162662235933027 1101 48-49 0.34900173717652905 1101 50-51 0.4197374073508442 1101 52-53 0.5600436929890193 1101 54-55 0.2569493496056019 1101 56-57 0.3182285165618026 1101 58-59 0.37433338421920226 1101 60-61 0.054815623221866616 1101 62-63 0.11078435539412723 1101 64-65 -0.047688393607636215 1101 66-67 0.5600893230188184 1101 68-69 0.769343468877441 1101 70-71 0.7169147602531858 1101 72-73 0.793617398774586 1101 74-75 0.5948795113114507 1101 76-77 0.9425632346711801 1101 78-79 0.5703598925490354 1101 80-81 0.6974989207871509 1101 82-83 0.8980888695399685 1101 84-85 0.9544765420419168 1101 86-87 0.7386468470857466 1101 88-89 0.84189828541232 1101 90-91 0.8993096104157665 1101 92-93 0.8960959840631268 1101 94-95 0.989863206281985 1101 96-97 0.9321127992795866 1101 98-99 0.989711912046161 1101 100-101 1.3111546353856003 1102 1 -0.1074878485040216 1102 2 0.04353420219603521 1102 3 -0.08544124292138022 1102 4 -0.0714671771072446 1102 5 -0.0371936914541493 1102 6 -0.09110533142769128 1102 7 -0.13505600439544452 1102 8 -0.13395159947805269 1102 9 -0.19813296056748442 1102 10-11 -0.1286499890596886 1102 12-13 9.915207014188354E-5 1102 14-15 -0.025628518935548072 1102 16-17 -0.01341735538419897 1102 18-19 -0.13502973487875636 1102 20-21 0.04108795731756487 1102 22-23 0.01119604671705332 1102 24-25 0.021919989983189225 1102 26-27 0.05738147748373734 1102 28-29 -0.1467438425147236 1102 30-31 0.07568359705454242 1102 32-33 -0.0969272101476335 1102 34-35 -0.019291161864138928 1102 36-37 -0.05601403412301664 1102 38-39 0.08518184559680719 1102 40-41 0.11116664281911426 1102 42-43 0.12045121109984791 1102 44-45 0.1493612074278019 1102 46-47 0.07864903319696026 1102 48-49 0.030656716497645675 1102 50-51 -0.035667898132878406 1102 52-53 0.06028044972041613 1102 54-55 0.11911000717787346 1102 56-57 0.07768243325044821 1102 58-59 0.31624977709886437 1102 60-61 0.32215432114515963 1102 62-63 0.4134538408641575 1102 64-65 0.3401248202618987 1102 66-67 0.5085495866364163 1102 68-69 0.5798067299916774 1102 70-71 0.552968366811843 1102 72-73 0.6901779917897635 1102 74-75 0.5315335770217544 1102 76-77 0.6069228859206959 1102 78-79 0.7781363202525569 1102 80-81 0.8704577116072478 1102 82-83 0.816474096204324 1102 84-85 0.7027124200005765 1102 86-87 0.8180511799472221 1102 88-89 1.044024012479447 1102 90-91 1.3095107327706508 1102 92-93 1.3795823797938276 1102 94-95 1.3623501544450605 1102 96-97 1.0916836368225233 1102 98-99 1.3954541159598293 1102 100-101 1.5796947137499462 1103 1 -0.0907219497549292 1103 2 -0.005603943206278217 1103 3 0.006024865016048153 1103 4 0.060192783602083466 1103 5 0.03955852057340792 1103 6 -0.13389711430079387 1103 7 -0.14395404904418285 1103 8 -0.16287300941144878 1103 9 -0.020149278732148446 1103 10-11 -0.08357948769806 1103 12-13 -0.11349225840616839 1103 14-15 -0.11062275804565758 1103 16-17 -0.10611221857396202 1103 18-19 -0.06785571444787308 1103 20-21 -0.235384487933878 1103 22-23 5.643730159974325E-4 1103 24-25 -0.13495752056169152 1103 26-27 -0.17804487863778462 1103 28-29 -0.24460306365849505 1103 30-31 -0.18089702155577214 1103 32-33 -0.0651637276880237 1103 34-35 -0.0390970275827911 1103 36-37 -0.040814199275224894 1103 38-39 -4.097632766359993E-4 1103 40-41 0.016777193257226486 1103 42-43 0.1637634513319668 1103 44-45 -0.14930323129260614 1103 46-47 0.22958049435838035 1103 48-49 0.15701039157004715 1103 50-51 0.43848986914861143 1103 52-53 0.32579363140058604 1103 54-55 0.15151794520825845 1103 56-57 0.20142343248836525 1103 58-59 0.02415439499334937 1103 60-61 0.12678223602474503 1103 62-63 0.44242870408072577 1103 64-65 0.2540559858383844 1103 66-67 0.45840437467655804 1103 68-69 0.5781597321224368 1103 70-71 0.5134070724849984 1103 72-73 0.7080404368870425 1103 74-75 0.9513603548712517 1103 76-77 1.2172142683130822 1103 78-79 1.1171970304472865 1103 80-81 0.7336464630256856 1103 82-83 0.9542947572555462 1103 84-85 0.9413536811093088 1103 86-87 1.0703183364403408 1103 88-89 1.0939418385293926 1103 90-91 1.3035430674827104 1103 92-93 1.4522635964776853 1103 94-95 1.6083837197555901 1103 96-97 1.9054046421866175 1103 98-99 2.0901623079519567 1103 100-101 2.361341905994765 1104 1 0.02550481838526153 1104 2 0.014762728857917295 1104 3 0.07994401004762253 1104 4 0.12657038266171128 1104 5 -0.028576273690816834 1104 6 0.03210985450749604 1104 7 0.002196014226839793 1104 8 -0.007589129428481556 1104 9 0.006190657577022307 1104 10-11 0.10144027325142702 1104 12-13 0.09093911202617022 1104 14-15 0.020076881519415224 1104 16-17 -0.11390714849606098 1104 18-19 -0.10905834364370293 1104 20-21 0.08173527121643076 1104 22-23 -0.08080635918864942 1104 24-25 0.011801263915025118 1104 26-27 -0.1961685779834852 1104 28-29 -0.03292251335053464 1104 30-31 -0.19317789691486098 1104 32-33 -0.03078897132643732 1104 34-35 0.04056748606996052 1104 36-37 -0.025506210334164336 1104 38-39 0.01385770222415772 1104 40-41 0.21960429844305196 1104 42-43 0.2229893258814286 1104 44-45 0.13210288470625642 1104 46-47 -0.17416509016391757 1104 48-49 0.0030893873899628943 1104 50-51 -0.0788451913936612 1104 52-53 0.1084756630300987 1104 54-55 0.0538511765882852 1104 56-57 -0.05331739621209053 1104 58-59 -0.1222670439875948 1104 60-61 0.0561810713581643 1104 62-63 0.13601254268264995 1104 64-65 0.08591099624361931 1104 66-67 0.2535531965161546 1104 68-69 0.20320096323858294 1104 70-71 0.13732381861526832 1104 72-73 0.3890975127463001 1104 74-75 0.5002799496968215 1104 76-77 0.32734782714313937 1104 78-79 0.39186523241078675 1104 80-81 0.32643896406021256 1104 82-83 0.3753918947665653 1104 84-85 0.5265206746262798 1104 86-87 0.5516263973484463 1104 88-89 0.6884600491889117 1104 90-91 0.8754951134456626 1104 92-93 1.3214799953669925 1104 94-95 1.4753046699603942 1104 96-97 1.5475830244875226 1104 98-99 1.4227264935549542 1104 100-101 1.512607626294864 1105 1 -0.11799085627919581 1105 2 -0.07146671690484396 1105 3 -0.045284602873557844 1105 4 -0.171376600066246 1105 5 -0.11653104076721377 1105 6 -0.18115827444484012 1105 7 -0.3040239312827211 1105 8 -0.14113988045070158 1105 9 -0.1889317785055269 1105 10-11 -0.1819492455000642 1105 12-13 -0.11526712717094512 1105 14-15 0.003252121432197441 1105 16-17 -0.1112338944291551 1105 18-19 -0.18439650243603722 1105 20-21 -0.10503206883822713 1105 22-23 -0.19466501048750473 1105 24-25 -0.24368806190181402 1105 26-27 -0.09088264965807724 1105 28-29 -0.22156479324389267 1105 30-31 -0.22122352324102934 1105 32-33 -0.17608529970875253 1105 34-35 -0.11520109278423973 1105 36-37 0.027009891967246347 1105 38-39 -0.23521058241514936 1105 40-41 -0.4453428014686409 1105 42-43 -0.35258738552431623 1105 44-45 -0.19132528375373425 1105 46-47 -0.3995831850074083 1105 48-49 0.08487840643650912 1105 50-51 -0.1335040820821618 1105 52-53 -0.06371537994703758 1105 54-55 0.18534964273683485 1105 56-57 0.08788848724868004 1105 58-59 0.19743844816318656 1105 60-61 0.3631632343188933 1105 62-63 0.3572659064837538 1105 64-65 0.6176269847093927 1105 66-67 0.5533871513418198 1105 68-69 0.7095607968376747 1105 70-71 0.663742474266666 1105 72-73 0.5484124691581336 1105 74-75 0.6894307233187611 1105 76-77 0.6298048144328874 1105 78-79 0.3359900372789326 1105 80-81 0.19296354544222538 1105 82-83 0.11686573140406153 1105 84-85 0.32824107840796657 1105 86-87 0.4312017946222504 1105 88-89 0.5887058211577241 1105 90-91 1.0542641401091473 1105 92-93 1.4666026407062205 1105 94-95 1.4963177024663246 1105 96-97 1.5683423332708628 1105 98-99 1.3998228269594293 1105 100-101 1.632136610437394 1106 1 -0.1623127050263733 1106 2 0.11385516536062568 1106 3 0.11785426341587524 1106 4 -0.008734413942207198 1106 5 -0.010497443248780769 1106 6 0.05794599487159502 1106 7 -0.05858775163685692 1106 8 -0.028126098337892813 1106 9 -0.07274289987526572 1106 10-11 -0.23464723958256428 1106 12-13 -0.07745963491824881 1106 14-15 -0.16423231541476468 1106 16-17 0.1034926724748857 1106 18-19 -0.03446117750665678 1106 20-21 0.035032118693578695 1106 22-23 -0.03832502529650128 1106 24-25 4.592168641437411E-5 1106 26-27 0.002749560203000101 1106 28-29 0.00580083648865326 1106 30-31 0.021416926025985106 1106 32-33 -0.12446027190380704 1106 34-35 0.03464167594868428 1106 36-37 0.015155081025653772 1106 38-39 0.11261750835239326 1106 40-41 0.07529723034326707 1106 42-43 -0.029358674973060772 1106 44-45 0.29898335878585414 1106 46-47 0.13133705973707066 1106 48-49 0.10498405000297595 1106 50-51 0.2718250218519742 1106 52-53 0.4245166519623709 1106 54-55 0.4349797988406685 1106 56-57 0.40347502691148307 1106 58-59 0.5004560087017929 1106 60-61 0.4581506497232901 1106 62-63 0.6341917609717029 1106 64-65 0.7431827027778866 1106 66-67 0.977783212637636 1106 68-69 0.8593186271680722 1106 70-71 0.8762880667634398 1106 72-73 1.0134144193133814 1106 74-75 0.7502675684551043 1106 76-77 0.837042708996524 1106 78-79 1.0919863837849562 1106 80-81 1.1357188710419734 1106 82-83 0.8388528177197614 1106 84-85 1.1571557103618666 1106 86-87 1.0965052570619527 1106 88-89 1.385602865148961 1106 90-91 1.5984138267131023 1106 92-93 1.8418460292228538 1106 94-95 1.8883800373506432 1106 96-97 2.036836661061489 1106 98-99 2.2751260151585555 1106 100-101 2.7398640244401182 1107 1 0.10226476608478663 1107 2 0.10382869285264462 1107 3 0.09055999961122652 1107 4 0.015046762834778349 1107 5 0.13261909806507077 1107 6 0.037261769914323395 1107 7 0.053410045151657926 1107 8 0.010035805353297178 1107 9 0.0814305439852987 1107 10-11 0.1404478972567702 1107 12-13 0.07392238690842845 1107 14-15 0.10846909362147983 1107 16-17 0.10697664217021696 1107 18-19 -0.022361712699400016 1107 20-21 0.13768648706180642 1107 22-23 0.17293982353196924 1107 24-25 0.20889544735697996 1107 26-27 0.11681185673553074 1107 28-29 0.22442589987951322 1107 30-31 0.2575971121666285 1107 32-33 0.2906319693785875 1107 34-35 0.1979088266640403 1107 36-37 -0.03132447173211972 1107 38-39 -0.051886522376094035 1107 40-41 -0.010017160008544579 1107 42-43 0.09675314205362184 1107 44-45 0.4044251051140364 1107 46-47 -0.06119361658576139 1107 48-49 0.06926759782050596 1107 50-51 0.07207264049903728 1107 52-53 0.17549592446582807 1107 54-55 0.38167081622452415 1107 56-57 0.13185439663447696 1107 58-59 0.03024324188390537 1107 60-61 0.21825274641386372 1107 62-63 0.3047447152890328 1107 64-65 0.3417869310998327 1107 66-67 0.5288394438680797 1107 68-69 0.5795631399663534 1107 70-71 0.5868977756143821 1107 72-73 0.7498160185019813 1107 74-75 1.0895888272620873 1107 76-77 0.9408728918614635 1107 78-79 1.0012426219712083 1107 80-81 1.086130234242681 1107 82-83 0.8946028033632984 1107 84-85 1.1237536816926195 1107 86-87 1.4862128462519841 1107 88-89 1.5565379844531861 1107 90-91 1.874186305495872 1107 92-93 1.8308977100613042 1107 94-95 1.900141619780598 1107 96-97 1.9186496113185711 1107 98-99 2.094078688978918 1107 100-101 2.346819669846008 1108 1 -0.03490817432514959 1108 2 -0.07095834247725463 1108 3 0.027376796185439645 1108 4 -0.03903345754225285 1108 5 -0.15834974831755488 1108 6 -0.08944501333682098 1108 7 -0.19477607399843322 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1.011204315253444 2315 90-91 0.7965385670131191 2315 92-93 0.5806450560630374 2315 94-95 0.5384730776922133 2315 96-97 0.6849589925500723 2315 98-99 1.012485777167985 2315 100-101 1.248135280695502 2316 1 -0.35570750114511895 2316 2 -0.5863729737175767 2316 3 -0.2869662575498175 2316 4 -0.15721974373312975 2316 5 -0.08938230521020074 2316 6 -0.34336941462360926 2316 7 -0.2289340032251701 2316 8 -0.049174816138517485 2316 9 -0.12547118334529017 2316 10-11 0.1852297385853916 2316 12-13 0.01861326851250311 2316 14-15 0.23851618291028842 2316 16-17 0.3978985723247135 2316 18-19 -0.35066121793636995 2316 20-21 -0.37646832892107085 2316 22-23 0.001664289708692479 2316 24-25 -0.09618220295260471 2316 26-27 0.3573542153953255 2316 28-29 0.3145971862634056 2316 30-31 0.002710965531591114 2316 32-33 0.3668383159441788 2316 34-35 0.22694450891622608 2316 36-37 0.08600814790794686 2316 38-39 -0.04134749107004154 2316 40-41 -0.34783217360728713 2316 42-43 -0.2015423124918314 2316 44-45 -0.09774219567874809 2316 46-47 0.13410577291715953 2316 48-49 -0.40788306620355996 2316 50-51 -0.256140064205546 2316 52-53 0.1697535267457866 2316 54-55 0.1891852106461407 2316 56-57 0.10994811227593004 2316 58-59 0.34174232324494014 2316 60-61 0.5382845009419555 2316 62-63 1.1437378652803503 2316 64-65 0.9436093806108552 2316 66-67 1.0547837613265614 2316 68-69 0.6756504251350357 2316 70-71 1.2138978045478943 2316 72-73 1.3034777351271778 2316 74-75 0.8715350398655239 2316 76-77 0.9584816456951515 2316 78-79 0.7269999566161935 2316 80-81 1.1036350082719615 2316 82-83 1.691318669053942 2316 84-85 1.5014309356131115 2316 86-87 1.3520174148533357 2316 88-89 1.3058173592000166 2316 90-91 1.6022257708045906 2316 92-93 1.473316587730423 2316 94-95 0.7761063715310748 2316 96-97 0.6244247400726941 2316 98-99 0.8889735518340771 2316 100-101 0.9059471074942937 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 16.0 4 50.0 5 88.0 6 256.0 7 555.0 8 1204.0 9 2057.0 10 3120.0 11 4969.0 12 6996.0 13 9635.0 14 12513.0 15 16553.0 16 21088.0 17 26317.0 18 23562.0 19 19253.0 20 19575.0 21 20636.0 22 22421.0 23 24359.0 24 27945.0 25 32610.0 26 38860.0 27 45174.0 28 53231.0 29 61564.0 30 71232.0 31 80744.0 32 92835.0 33 105849.0 34 119733.0 35 131616.0 36 131209.0 37 97479.0 38 29862.0 39 959.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.61397742606368 23.81927276188031 16.301660620735294 19.265089191320715 2 19.309315531736193 24.545896309580257 35.72481460151769 20.419973557165854 3 22.080430164298047 26.130254185983233 30.79063779142606 20.998677858292663 4 16.720631383499995 20.2766247853271 35.29358846071882 27.70915537045408 5 13.514125360314488 39.00282204870265 34.05417124164981 13.428881349333055 6 31.000425474492545 39.03437199743683 16.213822685533057 13.751379842537567 7 27.269154279115693 34.5843659877018 20.638388712611523 17.50809102057099 8 26.473362125986167 36.47621582838851 19.15940398176285 17.891018063862468 9 26.294913551989367 16.748283538561484 20.40058017908273 36.556222730366414 10-11 25.026868726866624 28.129372505079708 26.915625008065224 19.928133759988437 12-13 25.74332226707392 25.41321491305047 28.069449012369528 20.77401380750608 14-15 22.70300921812241 26.663977648210018 26.52992000047193 24.10309313319564 16-17 21.51363472461772 30.05859307309829 27.24393551369961 21.18383668858438 18-19 21.411837924451362 27.902978542665064 30.499073468457738 20.186110064425836 20-21 22.1089671883215 26.90211800759955 31.009237294572138 19.97967750950681 22-23 21.92036940561136 26.945534867926952 30.829580912371984 20.304514814089707 24-25 21.828575094488215 27.10708443159407 30.909730433445677 20.154610040472036 26-27 21.602585893504074 27.370113566059818 30.73808721454554 20.28921332589057 28-29 21.452276634725607 27.29107880994756 30.971177092401213 20.28546746292562 30-31 21.575736794659793 27.212891118276122 30.925890080394357 20.285482006669728 32-33 21.560606518682164 27.284713225030288 30.821681944175094 20.332998312112455 34-35 21.873186479497218 27.199433978820664 30.597220184155976 20.330159357526142 36-37 21.997895485100273 27.00581506814971 30.57943474529028 20.41685470145974 38-39 21.751145175514925 27.39185354731573 30.537178016169612 20.31982326099973 40-41 21.934229829481875 27.179628229268328 30.544641135465106 20.34150080578469 42-43 21.914016811383007 27.399619170109425 30.10244943531865 20.58391458318891 44-45 21.976674819762987 27.566105339380933 29.78049318244329 20.676726658412793 46-47 22.185434614451534 27.81003043211838 29.241453456261297 20.76308149716879 48-49 22.383026732269894 27.981336631929405 28.910006949910866 20.72562968588984 50-51 22.27591246581272 28.187980087498794 28.63513386961308 20.900973577075405 52-53 22.60032754899409 28.35898101439903 28.28867080146269 20.752020635144195 54-55 22.436884877810655 28.25279673896292 28.449901300241198 20.860417082985226 56-57 22.367164577515627 28.505869262085653 28.299657924407118 20.827308235991605 58-59 22.571827394510716 28.173895923187477 28.452408428966773 20.801868253335034 60-61 22.50387683213962 28.278494808400062 28.524267162586558 20.69336119687376 62-63 22.3555506723898 28.230675014913732 28.66057385226965 20.753200460426815 64-65 22.286604632436493 28.08854293789768 28.584770077861094 21.040082351804728 66-67 22.285498546234034 28.483268234015398 28.265369252130874 20.965863967619693 68-69 22.296080104237564 28.254013433785623 28.332324336919758 21.117582125057055 70-71 22.369892923481693 28.34224224320181 28.232739709158324 21.055125124158174 72-73 22.581892778953097 28.403703766518472 27.9653249349437 21.04907851958473 74-75 22.48916588564691 28.574188519857564 27.68478460445989 21.251860990035638 76-77 22.388290823982604 28.455542339873745 28.004590995131007 21.151575841012644 78-79 22.43526261804705 28.369562572402558 27.99836004285715 21.196814766693237 80-81 22.31436739611823 28.594429897362573 27.83672397913774 21.25447872738146 82-83 22.439351360598643 28.540568720868414 27.844323299150304 21.17575661938264 84-85 22.3813560165663 28.379653373044476 27.816357839773797 21.422632770615426 86-87 22.397692556703507 28.305114616339257 27.904527063348507 21.392665763608733 88-89 22.462693555868047 28.251469435519965 27.739425262861385 21.5464117457506 90-91 22.435626566610104 28.38068837557788 27.813519287509962 21.370165770302048 92-93 22.494649187653586 28.154767062224277 27.76456794379481 21.586015806327325 94-95 22.386889781459487 28.074827468987184 27.744033955371634 21.79424879418169 96-97 22.60854945643237 28.10915301080351 27.611193002456247 21.67110453030787 98-99 22.444996176628692 28.076117902890058 27.483956219633328 21.994929700847926 100-101 22.796017141453866 27.760984239803367 27.72436949257786 21.718629126164906 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 458.0 1 308.5 2 196.5 3 255.5 4 251.5 5 251.5 6 428.0 7 865.0 8 1382.5 9 1681.5 10 1642.0 11 1540.0 12 1673.0 13 1933.0 14 2127.5 15 2366.0 16 2594.5 17 2750.5 18 3017.0 19 3287.0 20 3456.5 21 3889.5 22 4578.0 23 5301.0 24 6338.5 25 7770.5 26 9723.0 27 12042.0 28 14123.0 29 16604.5 30 19507.0 31 22348.5 32 25596.0 33 28998.0 34 32331.0 35 35114.0 36 38278.0 37 41439.0 38 43094.0 39 43394.5 40 43826.5 41 45468.0 42 47373.5 43 48916.5 44 50177.5 45 51111.0 46 50503.5 47 49311.5 48 49382.0 49 48728.0 50 47077.0 51 44481.5 52 41327.5 53 39054.5 54 36840.0 55 33882.5 56 30270.5 57 26792.0 58 23697.5 59 21093.0 60 18152.5 61 14984.5 62 12185.5 63 9560.5 64 7328.5 65 5631.5 66 4299.0 67 3324.0 68 2441.5 69 1666.5 70 1188.0 71 886.0 72 683.0 73 521.0 74 400.5 75 294.0 76 199.0 77 123.0 78 93.0 79 70.0 80 34.5 81 18.0 82 14.5 83 6.5 84 3.5 85 2.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04984761819084116 2 0.0 3 0.0 4 0.0 5 0.0019172160842631219 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.8434770040991555E-4 12-13 0.004350605729674007 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.267821813937129E-4 24-25 8.479994218856116E-4 26-27 4.4243448098379735E-4 28-29 0.003060171826804598 30-31 4.055649409018142E-4 32-33 0.0 34-35 0.0 36-37 7.373908016396621E-5 38-39 6.636517214756959E-4 40-41 1.1060862024594934E-4 42-43 0.010839644784103035 44-45 1.4747816032793242E-4 46-47 0.0 48-49 3.6869540081983105E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.4747816032793242E-4 84-85 4.7930402106578047E-4 86-87 0.0 88-89 0.0 90-91 0.007373908016396622 92-93 0.0022490419450009696 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.008996167780003879 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1356133.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.17997702055674 #Duplication Level Percentage of deduplicated Percentage of total 1 80.03546639971498 48.16532528764389 2 11.568173940259808 13.92344883789277 3 3.6039141033082194 6.506504037634469 4 1.5615301292329198 3.7589138917657645 5 0.8317058526716505 2.5026019550821244 6 0.5179476512692756 1.8702046650741804 7 0.3426462740326317 1.4434311428224216 8 0.2286233417655315 1.1006837963050062 9 0.18764528987004384 1.0163240309155452 >10 1.0119495886111824 11.23974971614242 >50 0.0725297906828836 2.929416940323514 >100 0.03651523995446124 3.7727036068674438 >500 7.376719780741758E-4 0.2474174475212112 >1k 3.688359890370879E-4 0.5178453095696642 >5k 2.458906593580586E-4 1.005429334439515 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7313 0.539253893239085 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6276 0.46278646710905197 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4262 0.31427595965882404 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1651 0.12174322135070823 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.373908016396622E-5 0.0 14-15 0.0 0.0 0.0 1.1060862024594934E-4 3.686954008198311E-5 16-17 0.0 0.0 0.0 1.4747816032793245E-4 7.373908016396622E-5 18-19 0.0 0.0 0.0 2.949563206558649E-4 7.373908016396622E-5 20-21 0.0 0.0 0.0 3.3182586073784797E-4 7.373908016396622E-5 22-23 0.0 0.0 0.0 5.899126413117298E-4 1.1060862024594934E-4 24-25 0.0 0.0 0.0 9.217385020495778E-4 1.4747816032793245E-4 26-27 0.0 0.0 0.0 0.0014010425231153582 1.4747816032793245E-4 28-29 0.0 0.0 0.0 0.002838954586312699 1.4747816032793245E-4 30-31 0.0 0.0 0.0 0.005825387332953331 1.4747816032793245E-4 32-33 0.0 0.0 0.0 0.009549210881233625 1.4747816032793245E-4 34-35 0.0 0.0 0.0 0.01279373040844814 2.949563206558649E-4 36-37 0.0 0.0 0.0 0.018176683260417673 2.949563206558649E-4 38-39 0.0 0.0 0.0 0.028242067702799065 2.949563206558649E-4 40-41 0.0 0.0 0.0 0.0432111009760842 2.949563206558649E-4 42-43 0.0 0.0 0.0 0.057663960688221584 2.949563206558649E-4 44-45 0.0 0.0 0.0 0.07665177383044289 2.949563206558649E-4 46-47 0.0 0.0 0.0 0.10817523060053844 2.949563206558649E-4 48-49 0.0 0.0 0.0 0.13483190807981224 2.949563206558649E-4 50-51 0.0 0.0 0.0 0.16410632290490684 2.949563206558649E-4 52-53 0.0 0.0 0.0 0.19105795670483647 2.949563206558649E-4 54-55 0.0 0.0 0.0 0.22191776175345634 2.949563206558649E-4 56-57 0.0 0.0 0.0 0.27257650982610115 2.949563206558649E-4 58-59 0.0 0.0 0.0 0.3206543900930071 2.949563206558649E-4 60-61 0.0 0.0 0.0 0.3633493175079435 2.949563206558649E-4 62-63 0.0 0.0 0.0 0.4121645885764892 2.949563206558649E-4 64-65 0.0 0.0 0.0 0.4699760274250387 2.949563206558649E-4 66-67 0.0 0.0 0.0 0.539180154158921 2.949563206558649E-4 68-69 0.0 0.0 0.0 0.6201087946388739 2.949563206558649E-4 70-71 0.0 0.0 0.0 0.7112134281814542 2.949563206558649E-4 72-73 0.0 0.0 0.0 0.8074797973355121 2.949563206558649E-4 74-75 0.0 0.0 0.0 0.9218491106698237 2.949563206558649E-4 76-77 0.0 0.0 0.0 1.052404152100126 2.949563206558649E-4 78-79 0.0 0.0 0.0 1.1999560515082224 2.949563206558649E-4 80-81 0.0 0.0 0.0 1.3636568094722272 2.949563206558649E-4 82-83 0.0 0.0 0.0 1.5351739099336128 2.949563206558649E-4 84-85 0.0 0.0 0.0 1.7300662988069755 2.949563206558649E-4 86-87 0.0 0.0 0.0 1.923299558376649 2.949563206558649E-4 88-89 0.0 0.0 0.0 2.1335665454641983 2.949563206558649E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3300 0.0 60.77408 1 GTATCAA 6015 0.0 41.164417 1 TGGTATC 1255 0.0 33.3057 2 GTGGTAT 1435 0.0 30.468832 1 TATCAAC 8175 0.0 30.038788 2 ATCAACG 8340 0.0 29.501448 3 TCAACGC 8390 0.0 29.382248 4 CAACGCA 8645 0.0 28.46062 5 AACGCAG 9135 0.0 27.089987 6 ACGCAGA 10600 0.0 23.168415 7 CGCAGAG 10620 0.0 22.945868 8 TACATCG 170 3.1505606E-6 22.35222 5 GTGTAGC 520 0.0 21.934502 1 GAGTACT 7230 0.0 21.484295 12-13 GCAGAGT 11150 0.0 21.42914 9 AGTACTT 7320 0.0 21.15525 12-13 GTACTTT 7555 0.0 20.55857 14-15 AGAGTAC 10515 0.0 20.464472 10-11 CAGAGTA 10825 0.0 19.637075 10-11 GCACCGT 325 4.8748916E-10 18.99939 6 >>END_MODULE