FastQCFastQC Report
Thu 26 May 2016
SRR937439_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937439_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963679
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99680.5076186077256007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70570.35937645613157754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47960.24423543766572847No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32930.16769543290935024No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25700.1308767879067811No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20380.10378478356187545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA114250.034.4476241
GGTATCA72350.033.636951
TATCAAC139600.028.0431842
ATCAACG139050.028.0174373
TCAACGC139850.027.8253274
CAACGCA140900.027.6186755
AACGCAG145000.026.9284766
TACATGG82150.026.8346542
GTACATG82850.026.5627881
ACATGGG82350.025.0386943
ACGCAGA161700.024.0316987
CGCAGAG160750.023.9373058
CATGGGT28250.023.3784244
TAGGACG2052.6635462E-823.1774144
GAGTACT87550.022.3542512-13
AGAGTAC129600.022.30301510-11
GCAGAGT168500.022.130949
AGTACTT89500.021.94681512-13
TAGCCCT15150.021.6399024
GTACTTT93900.020.71395514-15
Produced by FastQC (version 0.11.5)