##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937439_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1963679 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.790033401589568 33.0 31.0 34.0 30.0 34.0 2 31.905718297135124 33.0 31.0 34.0 30.0 34.0 3 31.94227009607986 33.0 31.0 34.0 30.0 34.0 4 35.568213032781834 37.0 35.0 37.0 33.0 37.0 5 35.579481676995066 37.0 35.0 37.0 33.0 37.0 6 35.620419121455186 37.0 35.0 37.0 33.0 37.0 7 35.5646666283033 37.0 35.0 37.0 33.0 37.0 8 35.55320294202871 37.0 35.0 37.0 33.0 37.0 9 37.16512118324838 39.0 37.0 39.0 34.0 39.0 10-11 37.05088917282305 39.0 37.0 39.0 33.0 39.0 12-13 36.915953676746554 39.0 37.0 39.0 33.0 39.0 14-15 38.034652812399585 40.0 38.0 41.0 33.0 41.0 16-17 37.96380034618693 40.0 38.0 41.0 33.0 41.0 18-19 37.91008153572962 40.0 38.0 41.0 33.0 41.0 20-21 37.78810997113072 40.0 37.5 41.0 33.0 41.0 22-23 37.85779473121625 40.0 38.0 41.0 33.0 41.0 24-25 37.83377629439435 40.0 38.0 41.0 33.0 41.0 26-27 37.753606113830216 40.0 37.5 41.0 33.0 41.0 28-29 37.615333259662094 40.0 37.0 41.0 32.5 41.0 30-31 37.36094595909006 39.5 37.0 41.0 32.0 41.0 32-33 37.09680120834413 39.0 36.0 41.0 31.5 41.0 34-35 37.08307646005279 39.0 36.0 41.0 31.0 41.0 36-37 36.94850838655401 39.0 36.0 41.0 31.0 41.0 38-39 36.79311282546689 39.0 36.0 41.0 31.0 41.0 40-41 36.55189748426295 39.0 35.5 41.0 30.0 41.0 42-43 36.26666833021079 39.0 35.0 41.0 30.0 41.0 44-45 35.98233214288079 39.0 35.0 40.5 29.5 41.0 46-47 35.54310913341743 39.0 35.0 40.0 27.5 41.0 48-49 35.1677351542691 39.0 35.0 40.0 26.0 41.0 50-51 34.19571987071207 37.5 34.0 39.5 24.0 40.5 52-53 34.29061318066751 38.0 34.0 39.5 24.0 40.5 54-55 34.59385826298494 38.0 34.0 40.0 24.0 41.0 56-57 34.32040216349006 38.0 34.0 40.0 23.0 41.0 58-59 34.04956920148354 38.0 34.0 40.0 22.5 41.0 60-61 33.714973272108125 38.0 33.0 40.0 21.5 41.0 62-63 33.337139114896075 37.0 33.0 40.0 19.5 41.0 64-65 32.91251141352532 36.5 32.0 39.5 18.0 41.0 66-67 32.470875840705126 36.0 31.5 39.0 14.5 40.5 68-69 31.965524405974705 35.5 31.0 39.0 10.0 40.0 70-71 31.477617013778726 35.0 31.0 38.5 7.5 40.0 72-73 30.977887169949874 35.0 30.0 37.5 6.0 40.0 74-75 30.42339990395579 34.5 29.5 37.0 2.0 39.0 76-77 29.888684454027363 34.0 29.0 36.5 2.0 39.0 78-79 29.32272153442594 34.0 29.0 36.0 2.0 38.5 80-81 28.729286202072743 34.0 28.0 35.5 2.0 37.0 82-83 28.14476245862995 34.0 26.5 35.0 2.0 37.0 84-85 27.540593447299685 33.0 26.0 35.0 2.0 36.0 86-87 26.928496969209327 33.0 25.5 35.0 2.0 36.0 88-89 26.340283977167346 32.0 24.5 35.0 2.0 35.5 90-91 25.745231527148785 32.0 22.0 34.5 2.0 35.0 92-93 25.045525006887583 31.5 19.0 34.0 2.0 35.0 94-95 24.235625069066785 31.0 12.0 34.0 2.0 35.0 96-97 23.351672803956248 31.0 2.0 34.0 2.0 35.0 98-99 22.13414055963322 30.5 2.0 34.0 2.0 35.0 100-101 18.988242222888772 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3638423818142229 1101 2 0.3777210421232944 1101 3 0.3210103348458908 1101 4 0.1569362312645879 1101 5 0.24167533420739318 1101 6 0.2164649306216191 1101 7 0.2891471079640624 1101 8 0.2021341787215789 1101 9 0.1461187477665291 1101 10-11 0.30196910038904434 1101 12-13 0.40253401950243983 1101 14-15 0.5692600804300412 1101 16-17 0.5467331036883962 1101 18-19 0.5674231774347476 1101 20-21 0.6754337211668044 1101 22-23 0.5779082013054406 1101 24-25 0.49342885057913577 1101 26-27 0.5308217659292467 1101 28-29 0.5154517134290657 1101 30-31 0.46101327035152195 1101 32-33 0.5419597921541595 1101 34-35 0.6547541956838501 1101 36-37 0.6946764965418808 1101 38-39 0.7576246291571849 1101 40-41 0.8101148745350599 1101 42-43 0.9069515267078359 1101 44-45 0.9899312011697674 1101 46-47 1.1867103211845915 1101 48-49 1.183061359935408 1101 50-51 0.9298986167021042 1101 52-53 0.9579667914930283 1101 54-55 1.1867180855618713 1101 56-57 1.3696230430127372 1101 58-59 1.2035571240697323 1101 60-61 1.2506290990317979 1101 62-63 1.317083884334025 1101 64-65 1.0899007713310098 1101 66-67 1.3447360637738157 1101 68-69 1.4623701656464867 1101 70-71 1.4649149361198859 1101 72-73 1.444977691394584 1101 74-75 1.620296354457377 1101 76-77 1.669089163834954 1101 78-79 1.6916667041378446 1101 80-81 1.738013728886422 1101 82-83 1.800092970972667 1101 84-85 1.9545197861098167 1101 86-87 1.8174567590419386 1101 88-89 1.9297835966420642 1101 90-91 1.963048073279655 1101 92-93 2.18130879973517 1101 94-95 2.3452790647039627 1101 96-97 2.4918549925091504 1101 98-99 2.8030767317258665 1101 100-101 2.778555857875883 1102 1 0.1768130294063326 1102 2 0.2361246693257577 1102 3 0.1488562391254611 1102 4 0.20231508060603431 1102 5 0.1972641442946852 1102 6 0.10634326044187503 1102 7 0.06194813587845971 1102 8 0.017348536743057252 1102 9 0.2714574932641227 1102 10-11 0.1679981944381197 1102 12-13 0.2505024738481936 1102 14-15 0.35580469735810993 1102 16-17 0.4232896724073143 1102 18-19 0.3421215683208487 1102 20-21 0.27493194867006565 1102 22-23 0.3751090409931521 1102 24-25 0.44069982239214767 1102 26-27 0.322427023854587 1102 28-29 0.32596471741745603 1102 30-31 0.4537345822794592 1102 32-33 0.4775696806975702 1102 34-35 0.5919262061510508 1102 36-37 0.5525738655520129 1102 38-39 0.42810446056404317 1102 40-41 0.5810748263310828 1102 42-43 0.6276136757614594 1102 44-45 0.7192722007293852 1102 46-47 0.9739658561663305 1102 48-49 0.8155808845724408 1102 50-51 0.6833163716891022 1102 52-53 0.7404677048065267 1102 54-55 0.7818128911695084 1102 56-57 0.8239101387448216 1102 58-59 0.9435368780552054 1102 60-61 1.0203559158571238 1102 62-63 0.9322224168473454 1102 64-65 0.8482635743904297 1102 66-67 1.00372518208016 1102 68-69 1.1021698879646813 1102 70-71 1.2223837271084683 1102 72-73 1.235417962416868 1102 74-75 1.1934958976065353 1102 76-77 1.2406999512427461 1102 78-79 1.191530839319455 1102 80-81 1.4581991649542587 1102 82-83 1.5072336592077669 1102 84-85 1.4339102792818572 1102 86-87 1.4796325543147901 1102 88-89 1.4334847268216961 1102 90-91 1.6452774079172912 1102 92-93 1.755111000400177 1102 94-95 1.838986582686644 1102 96-97 1.603333693434454 1102 98-99 2.0629053652147746 1102 100-101 2.1043823365056404 1103 1 0.2540060342684569 1103 2 0.2866954331081182 1103 3 0.3014130990992463 1103 4 0.23281864785010953 1103 5 0.25459358565901624 1103 6 0.17768104674023277 1103 7 0.21003547529571165 1103 8 0.26077468984767194 1103 9 0.28777840947562794 1103 10-11 0.28100069621547874 1103 12-13 0.27875295147528334 1103 14-15 0.41694664117619595 1103 16-17 0.36868858267491333 1103 18-19 0.2719200827693271 1103 20-21 0.3550449945709886 1103 22-23 0.1659910345819 1103 24-25 0.22448564205888744 1103 26-27 0.17880045421279078 1103 28-29 0.029589243633616036 1103 30-31 0.24271174368691106 1103 32-33 0.34025585356475574 1103 34-35 0.5217355999489683 1103 36-37 0.6177142067946377 1103 38-39 0.6934529757930008 1103 40-41 0.7809028143562315 1103 42-43 0.5111464559508221 1103 44-45 0.9245527531354512 1103 46-47 0.8271409942985954 1103 48-49 0.8347686276135633 1103 50-51 0.7293577784930463 1103 52-53 0.7753551768572393 1103 54-55 1.1347544780503327 1103 56-57 0.9597491045065567 1103 58-59 1.0233882283007247 1103 60-61 1.0097972707240075 1103 62-63 1.0653055960959747 1103 64-65 1.094807015869229 1103 66-67 1.343838596932926 1103 68-69 1.4134887394648707 1103 70-71 1.5754129400537558 1103 72-73 1.5261755530099208 1103 74-75 1.4532919239951063 1103 76-77 1.3777148327737265 1103 78-79 1.426781516085942 1103 80-81 1.505603801543785 1103 82-83 1.6472884911289114 1103 84-85 1.927771251602426 1103 86-87 2.0953538291664167 1103 88-89 2.0996142847369654 1103 90-91 2.0757710181915634 1103 92-93 2.4322693805501885 1103 94-95 2.118722091511451 1103 96-97 2.3679255026087738 1103 98-99 2.506414698041038 1103 100-101 2.2200949788300015 1104 1 0.3014344015545447 1104 2 0.3861317105136486 1104 3 0.19597250094568963 1104 4 0.37368600426118803 1104 5 0.33922651898559764 1104 6 0.36274746136326286 1104 7 0.43191685725996365 1104 8 0.4334147344053818 1104 9 0.39978029980289165 1104 10-11 0.4648427582474639 1104 12-13 0.5237929521504014 1104 14-15 0.6488985165946275 1104 16-17 0.625593059262421 1104 18-19 0.6590222560830838 1104 20-21 0.5896059154304041 1104 22-23 0.5846464830564173 1104 24-25 0.6671281339152912 1104 26-27 0.657886599126634 1104 28-29 0.7509671009251591 1104 30-31 0.7698873078314534 1104 32-33 0.7541496182977738 1104 34-35 0.894157497364958 1104 36-37 0.9835537146344322 1104 38-39 0.9760294749744247 1104 40-41 1.0850570335554366 1104 42-43 1.0553258232537885 1104 44-45 1.1100762781457192 1104 46-47 1.0753491215806292 1104 48-49 1.1817076758112748 1104 50-51 1.1799441456328594 1104 52-53 1.029816574236662 1104 54-55 1.116165556718336 1104 56-57 1.2195481447153966 1104 58-59 1.3884372867939803 1104 60-61 1.3864027830533274 1104 62-63 1.4262019825328807 1104 64-65 1.4635291321355979 1104 66-67 1.5847280352919455 1104 68-69 1.315883716595284 1104 70-71 1.371415826178417 1104 72-73 1.4384497404786245 1104 74-75 1.4091863976008518 1104 76-77 1.4741644212337235 1104 78-79 1.4998776864854442 1104 80-81 1.5863562193061078 1104 82-83 1.6252086529319243 1104 84-85 1.534519888710225 1104 86-87 1.60953831610572 1104 88-89 1.7041146419863153 1104 90-91 1.9532619181786224 1104 92-93 2.0016946029168103 1104 94-95 1.9140245438735164 1104 96-97 2.0230814264780967 1104 98-99 1.971622268351645 1104 100-101 1.7001396490635337 1105 1 0.2646445946719602 1105 2 0.1327587813526172 1105 3 0.14192533697930187 1105 4 0.17927988024691643 1105 5 0.27746290748104485 1105 6 0.2825351954323523 1105 7 0.35467471718972376 1105 8 0.228842873068011 1105 9 0.29959723930517157 1105 10-11 0.3012425304310469 1105 12-13 0.31290151071133465 1105 14-15 0.45235069006507445 1105 16-17 0.37987646429299105 1105 18-19 0.3972106416579919 1105 20-21 0.4318733206575516 1105 22-23 0.4409703193585557 1105 24-25 0.4011250396136887 1105 26-27 0.5256124454084699 1105 28-29 0.3875169809617347 1105 30-31 0.4576491519158665 1105 32-33 0.5562527517388673 1105 34-35 0.80289029924365 1105 36-37 0.794966518689499 1105 38-39 0.7347764056834833 1105 40-41 0.6895406116422294 1105 42-43 0.7364464588931909 1105 44-45 0.7815116444510011 1105 46-47 0.6693773652002761 1105 48-49 0.7160203301533912 1105 50-51 0.7059664235513381 1105 52-53 0.740352381607174 1105 54-55 0.8228534517332236 1105 56-57 0.8449103605372272 1105 58-59 0.8158390124419341 1105 60-61 0.9593935492922654 1105 62-63 0.9342940287911929 1105 64-65 0.986759210923573 1105 66-67 0.9933090180617938 1105 68-69 0.8403537977000362 1105 70-71 1.1987380442468272 1105 72-73 1.187826667835374 1105 74-75 1.0816693596355442 1105 76-77 1.0543843658767926 1105 78-79 1.2183723693063655 1105 80-81 1.2577187475418299 1105 82-83 1.3353037022248664 1105 84-85 1.3949384531157527 1105 86-87 1.3552026958325172 1105 88-89 1.305981095579689 1105 90-91 1.4295708972697092 1105 92-93 1.47338494634392 1105 94-95 1.7050694542475888 1105 96-97 1.768797582663975 1105 98-99 1.594405753240821 1105 100-101 1.5687454757580745 1106 1 -0.02107587556969648 1106 2 -0.5257740892985865 1106 3 -0.05896845389679228 1106 4 0.03988454521356033 1106 5 0.08899722240567343 1106 6 0.029667642450881715 1106 7 0.16633499528929008 1106 8 0.07788265813258022 1106 9 0.181129264464424 1106 10-11 -0.22865585927450383 1106 12-13 -0.10428735111879917 1106 14-15 -0.02601741658061485 1106 16-17 0.09171796767260787 1106 18-19 0.26899186526895846 1106 20-21 0.09721263624198428 1106 22-23 0.15560218089608213 1106 24-25 0.21962970807496163 1106 26-27 0.2207863867310138 1106 28-29 0.425156498869633 1106 30-31 0.3395170788749553 1106 32-33 0.4434866317123962 1106 34-35 0.5175637573161751 1106 36-37 0.35552442341334967 1106 38-39 0.5201697107016159 1106 40-41 0.7139260912959173 1106 42-43 0.42528142731372753 1106 44-45 0.560343911052712 1106 46-47 0.6646178870581494 1106 48-49 0.8688156173050956 1106 50-51 0.697935553967298 1106 52-53 0.88808423599896 1106 54-55 0.9475855517923222 1106 56-57 0.6666736415675913 1106 58-59 0.5927654014402108 1106 60-61 0.6511480989621816 1106 62-63 0.7560819547905666 1106 64-65 0.69266649263826 1106 66-67 1.05063021685568 1106 68-69 0.9912721553071471 1106 70-71 1.0684809072463572 1106 72-73 1.0226446106246208 1106 74-75 0.8907222260931924 1106 76-77 1.124409459410316 1106 78-79 1.1683185676438583 1106 80-81 1.118246478478227 1106 82-83 1.145624937008229 1106 84-85 1.1015262750064316 1106 86-87 1.0082173101687388 1106 88-89 1.3123533196303612 1106 90-91 1.2584241055752443 1106 92-93 1.415684554058604 1106 94-95 1.3924349826904923 1106 96-97 1.468447780580032 1106 98-99 1.756217969530283 1106 100-101 0.828463547744331 1107 1 0.2584949031592032 1107 2 0.30935710432028074 1107 3 0.1607506504082572 1107 4 0.3103981787304022 1107 5 0.08517666416906877 1107 6 0.19552326751360738 1107 7 0.2140468375059541 1107 8 0.15612420331121513 1107 9 0.28364767059526486 1107 10-11 0.2587055725994034 1107 12-13 0.26021207108765054 1107 14-15 0.33224430231175006 1107 16-17 0.3856186432509219 1107 18-19 0.3958085788375527 1107 20-21 0.29400755008878576 1107 22-23 0.39200244576719 1107 24-25 0.3962258172058881 1107 26-27 0.28267575762849617 1107 28-29 0.25346182281248275 1107 30-31 0.2961894309628903 1107 32-33 0.23630096570229853 1107 34-35 0.38460241537733 1107 36-37 0.3470352596356534 1107 38-39 0.41000990775136614 1107 40-41 0.44132822383351566 1107 42-43 0.5045271726393779 1107 44-45 0.5326804678068413 1107 46-47 0.6363120304574821 1107 48-49 0.5105829142461289 1107 50-51 0.3252203055441072 1107 52-53 0.387129236612914 1107 54-55 0.7423824942243442 1107 56-57 0.7838307459051634 1107 58-59 0.8452154871981108 1107 60-61 0.9082100201523744 1107 62-63 0.8295573339448055 1107 64-65 0.7353745754121661 1107 66-67 0.8994061411711698 1107 68-69 0.9554098467906194 1107 70-71 0.9633519171487457 1107 72-73 1.073473201901706 1107 74-75 1.1379982420818955 1107 76-77 1.2756500755215896 1107 78-79 1.2758373690628595 1107 80-81 1.2139153256777675 1107 82-83 1.3702397272896363 1107 84-85 1.2213452666683757 1107 86-87 1.1251872834068912 1107 88-89 1.3352997976128123 1107 90-91 1.1425614998917304 1107 92-93 1.3607329109774646 1107 94-95 1.495037913205561 1107 96-97 1.2827900406975843 1107 98-99 1.4119640296847322 1107 100-101 1.4393570167428074 1108 1 0.1884662791186571 1108 2 0.15825069008691983 1108 3 0.1166926782613551 1108 4 0.23898067665327716 1108 5 0.1915863395635995 1108 6 0.27626897716321963 1108 7 0.1525657061921919 1108 8 0.12359463512010649 1108 9 0.15644519522782474 1108 10-11 0.20261879669777016 1108 12-13 0.28009881208826926 1108 14-15 0.2700400818936117 1108 16-17 0.27521032743320717 1108 18-19 0.3164270039683643 1108 20-21 0.27938070443722296 1108 22-23 0.3503315467014403 1108 24-25 0.3090237455442093 1108 26-27 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38-39 0.20075018433990266 2310 40-41 0.20011787650960144 2310 42-43 0.1262847808597627 2310 44-45 0.38869479894207615 2310 46-47 0.4606047508497966 2310 48-49 0.45353753613478176 2310 50-51 0.5510143556971414 2310 52-53 0.5490323233287526 2310 54-55 0.44402074417356374 2310 56-57 0.6079953885619176 2310 58-59 0.4396480074074631 2310 60-61 0.6083877810227634 2310 62-63 0.5583092588217013 2310 64-65 0.4755246765808536 2310 66-67 0.6510555661148558 2310 68-69 0.6885283954578263 2310 70-71 0.7405799926365724 2310 72-73 0.823534272080245 2310 74-75 0.8517173608222564 2310 76-77 1.063192013558261 2310 78-79 1.0817413921880998 2310 80-81 1.2960007492863923 2310 82-83 1.425591362168003 2310 84-85 1.7491369690123229 2310 86-87 1.731318347643814 2310 88-89 1.8218303244321632 2310 90-91 1.9258548195351644 2310 92-93 2.0507755668007803 2310 94-95 2.4285792456296242 2310 96-97 2.8015495060451343 2310 98-99 3.0220099468252215 2310 100-101 3.344056366981551 2311 1 0.010928716647594428 2311 2 -0.030295842178176713 2311 3 0.01961827060797816 2311 4 -0.07368690054431681 2311 5 -0.045617302887613675 2311 6 -0.044113752881969504 2311 7 -0.05156159045214537 2311 8 -0.003914493730256652 2311 9 -0.05584770914063597 2311 10-11 0.005265364950133744 2311 12-13 0.00453204343722291 2311 14-15 0.03602048296119165 2311 16-17 0.15559997243668988 2311 18-19 0.08038956984778167 2311 20-21 0.15245329798874963 2311 22-23 0.015800787419365747 2311 24-25 0.013480055412948388 2311 26-27 0.08321063748807944 2311 28-29 0.05312330841291413 2311 30-31 0.0628084887475282 2311 32-33 0.15378681010766115 2311 34-35 0.19819669960066477 2311 36-37 0.3211807564453508 2311 38-39 0.18319170591003342 2311 40-41 0.23102488240159147 2311 42-43 0.07075434663713764 2311 44-45 0.16477732562063352 2311 46-47 0.14534501104458286 2311 48-49 0.24868238800702613 2311 50-51 0.11979052102088872 2311 52-53 0.1340033153556348 2311 54-55 0.20735862201268418 2311 56-57 -0.37779971429144155 2311 58-59 0.06178168387531713 2311 60-61 0.2952151673332821 2311 62-63 0.3247727260149631 2311 64-65 0.5236412732533253 2311 66-67 0.6665665170271922 2311 68-69 0.5207314354702142 2311 70-71 0.5867724374240275 2311 72-73 0.5560722402361726 2311 74-75 0.6607573208076722 2311 76-77 0.8934185235398076 2311 78-79 0.9161556539232336 2311 80-81 1.1363713001688929 2311 82-83 1.1358888176380795 2311 84-85 1.3485143510530015 2311 86-87 1.3974575663154738 2311 88-89 1.6082333857637892 2311 90-91 1.4943148960484542 2311 92-93 1.489216745983036 2311 94-95 1.4901914645570749 2311 96-97 1.6892402817916725 2311 98-99 1.863286065650616 2311 100-101 2.1871841202590865 2312 1 0.20694512643212093 2312 2 0.13230730883077158 2312 3 0.16878097049531604 2312 4 0.17160310199206918 2312 5 0.11318334359419424 2312 6 0.05004313692709417 2312 7 0.17732688553777365 2312 8 0.23812540410674643 2312 9 0.30802569603712016 2312 10-11 0.25564729649148177 2312 12-13 0.296320367834241 2312 14-15 0.50511101400825 2312 16-17 0.486471395699418 2312 18-19 0.526830380781341 2312 20-21 0.47348238967001777 2312 22-23 0.4148916723798237 2312 24-25 0.45748608963637594 2312 26-27 0.4585969929468945 2312 28-29 0.4414388575980368 2312 30-31 0.5675457670355328 2312 32-33 0.6349707094075043 2312 34-35 0.613477795316534 2312 36-37 0.592549162596363 2312 38-39 0.538047545186167 2312 40-41 0.340305671392521 2312 42-43 0.4197716471037083 2312 44-45 0.4724202329012144 2312 46-47 0.7101722128283043 2312 48-49 0.8025160074203583 2312 50-51 0.9516387452604178 2312 52-53 0.9014853100115445 2312 54-55 1.020069274216091 2312 56-57 1.115294403078039 2312 58-59 1.0997794483223586 2312 60-61 1.0641098663004485 2312 62-63 0.9783021273704477 2312 64-65 1.0884187796975056 2312 66-67 0.9116195450512379 2312 68-69 1.2384542387002604 2312 70-71 1.1906240474350547 2312 72-73 1.4025024337678147 2312 74-75 1.5728063024218706 2312 76-77 1.6729229563051113 2312 78-79 1.7111060470620352 2312 80-81 1.6899012913800462 2312 82-83 1.5776743402207032 2312 84-85 1.7641667609083065 2312 86-87 1.9704666559912276 2312 88-89 2.166693095672777 2312 90-91 2.3248478896249267 2312 92-93 2.643845785757005 2312 94-95 2.72341139813933 2312 96-97 3.1288818265056797 2312 98-99 3.3799693957397423 2312 100-101 3.8274156680993308 2313 1 0.09622605478156387 2313 2 0.01670271270269552 2313 3 -0.07847475328080833 2313 4 0.04373408593983186 2313 5 0.07517832548909809 2313 6 -0.06644818118085283 2313 7 0.031113273927708462 2313 8 0.21327010230763932 2313 9 0.22960212234795563 2313 10-11 0.2138487620062719 2313 12-13 0.06958612782055695 2313 14-15 0.21999639592596054 2313 16-17 0.41625712831424977 2313 18-19 0.43171590525885506 2313 20-21 0.27609337247729826 2313 22-23 0.2712747875966528 2313 24-25 0.3209943438490157 2313 26-27 0.27655168744123415 2313 28-29 0.28973241674609795 2313 30-31 0.2152626717770758 2313 32-33 0.40205507629941906 2313 34-35 0.25855092849591443 2313 36-37 0.21637945940587855 2313 38-39 0.32561134069838005 2313 40-41 0.2931554418712423 2313 42-43 0.2784250271966542 2313 44-45 0.35694322779757215 2313 46-47 0.3321506577859594 2313 48-49 0.335758815278254 2313 50-51 0.17205829876311185 2313 52-53 0.11706353257590507 2313 54-55 0.38212056723351395 2313 56-57 0.4077494254599259 2313 58-59 0.46271176092749755 2313 60-61 0.6028283962237495 2313 62-63 0.5990702395935301 2313 64-65 0.5505427782148828 2313 66-67 0.5346750293268911 2313 68-69 0.5482595020082179 2313 70-71 0.5283118699766156 2313 72-73 0.4760818253225736 2313 74-75 0.5991256934063891 2313 76-77 0.4571095100644129 2313 78-79 0.6695263418757094 2313 80-81 0.7446128928968818 2313 82-83 0.9269679563926871 2313 84-85 1.1291683290661503 2313 86-87 1.5501447559541646 2313 88-89 1.7501518816334851 2313 90-91 1.6288637992990367 2313 92-93 1.8200147761484757 2313 94-95 2.305897213438847 2313 96-97 2.2814849685237597 2313 98-99 2.499381051429303 2313 100-101 2.8286452464074294 2314 1 -0.051824300416402735 2314 2 0.03817768871245164 2314 3 0.0762359344474568 2314 4 0.12275777489815454 2314 5 0.14191035000481378 2314 6 0.2613825194529156 2314 7 0.24341241122782264 2314 8 0.16299081908515234 2314 9 0.06499938775657199 2314 10-11 0.14815048277998244 2314 12-13 0.12848381016717525 2314 14-15 0.26522201098364917 2314 16-17 0.2154347581373557 2314 18-19 0.2547432191461567 2314 20-21 0.281875644249709 2314 22-23 0.26285863009253774 2314 24-25 0.23016057459899741 2314 26-27 0.2684445266644815 2314 28-29 0.46718787317755783 2314 30-31 0.3231360388687463 2314 32-33 0.34536752121674397 2314 34-35 0.2922656741044207 2314 36-37 0.3277600283157014 2314 38-39 0.2747683312007325 2314 40-41 0.20743553880488008 2314 42-43 0.3296025360306416 2314 44-45 0.3351554000149193 2314 46-47 0.5669261933110974 2314 48-49 0.36491066967377606 2314 50-51 0.28585805702210365 2314 52-53 0.35648588069008014 2314 54-55 0.4794450470099392 2314 56-57 0.6589765161104424 2314 58-59 0.5777964355201348 2314 60-61 0.6133627529827095 2314 62-63 0.6605454646985365 2314 64-65 0.9641245188213219 2314 66-67 0.9767962651714583 2314 68-69 1.0161825576715167 2314 70-71 1.1414491534861533 2314 72-73 1.1253990077943357 2314 74-75 1.2296059371876105 2314 76-77 1.1221054593011743 2314 78-79 0.9895025354867713 2314 80-81 1.4443801667628762 2314 82-83 1.5774855407381203 2314 84-85 1.7782073551748887 2314 86-87 1.9186186086629533 2314 88-89 1.8401974423409655 2314 90-91 2.1420880882210582 2314 92-93 2.298567675083415 2314 94-95 2.3273143637654563 2314 96-97 2.7756365678931907 2314 98-99 2.9869882661766916 2314 100-101 3.3437727416597944 2315 1 -0.16512155967656028 2315 2 -0.06883977000659414 2315 3 -0.07798679464515246 2315 4 -0.0030299299900491405 2315 5 -0.14220209433717912 2315 6 0.017176411934386238 2315 7 -0.2071826896542106 2315 8 -0.26516515679536923 2315 9 -0.1538436536275114 2315 10-11 -0.06411551093536616 2315 12-13 -0.04417248722553779 2315 14-15 0.039588995927175574 2315 16-17 0.01204007461454637 2315 18-19 -0.027816260610705967 2315 20-21 0.09314555677412528 2315 22-23 -0.032111827182731645 2315 24-25 -0.14330860269375023 2315 26-27 -0.040117403864613266 2315 28-29 0.006093275795755915 2315 30-31 -0.002005608486967958 2315 32-33 -0.07734094607999964 2315 34-35 -0.2374408398540666 2315 36-37 -0.06741000917383388 2315 38-39 -0.3037843786011436 2315 40-41 -0.22626864570673177 2315 42-43 -0.02635939207194582 2315 44-45 -0.18371615282833886 2315 46-47 -0.36594327273600413 2315 48-49 -0.2431119696380719 2315 50-51 -0.2132562678629455 2315 52-53 -0.3594435322165097 2315 54-55 -0.38403056052678863 2315 56-57 -0.201549671968138 2315 58-59 -0.13255019102827958 2315 60-61 -0.1762784879330468 2315 62-63 -0.2256932065461399 2315 64-65 -0.005721883275178641 2315 66-67 0.06957861961136302 2315 68-69 0.08019624317192253 2315 70-71 0.18123598483176906 2315 72-73 0.10962307146587946 2315 74-75 0.18025745436121454 2315 76-77 0.14669810941096983 2315 78-79 0.16365538117305078 2315 80-81 0.21243944633289757 2315 82-83 0.3453009563381322 2315 84-85 0.7418667706293149 2315 86-87 0.8557296092331867 2315 88-89 1.013533768366468 2315 90-91 1.0735792981988546 2315 92-93 1.1343362105650172 2315 94-95 1.389506955639085 2315 96-97 1.7061735539286431 2315 98-99 1.9800981990837698 2315 100-101 2.5770146765566047 2316 1 -0.34936466674798083 2316 2 -0.40042064956706724 2316 3 -0.5802464599361876 2316 4 -0.37708492150839135 2316 5 -0.2335039653653581 2316 6 -0.22092268419950045 2316 7 -0.09024089660663748 2316 8 -0.2613585385711943 2316 9 -0.5115467703199172 2316 10-11 -0.6512639019524045 2316 12-13 -0.33298176387945944 2316 14-15 -0.6303599875399257 2316 16-17 -0.5875282470301286 2316 18-19 -0.15235166836319536 2316 20-21 -0.3619320440563172 2316 22-23 -0.4588936080774886 2316 24-25 -0.3531397291084133 2316 26-27 -0.5106430134045752 2316 28-29 -0.30870405058346506 2316 30-31 -0.3373260125682904 2316 32-33 -0.2883987479383734 2316 34-35 -0.24434642762022207 2316 36-37 -0.31781763506946703 2316 38-39 -0.2625578297493263 2316 40-41 -0.4110598956750806 2316 42-43 -0.530206519701899 2316 44-45 -0.3664756029131624 2316 46-47 -0.481253296282091 2316 48-49 -0.5419333991137236 2316 50-51 -0.652568114332297 2316 52-53 -0.47026650611326204 2316 54-55 -0.3763242790370569 2316 56-57 -0.49691134500659473 2316 58-59 -0.6559418410270084 2316 60-61 -0.7600753585814601 2316 62-63 -0.4325082811087668 2316 64-65 -0.45018032315668677 2316 66-67 -0.26714568856394294 2316 68-69 -0.34159022162286945 2316 70-71 -0.31423582315364484 2316 72-73 -0.4019297046943322 2316 74-75 -0.2795040465122689 2316 76-77 -0.39131427127616547 2316 78-79 -0.08923534470486771 2316 80-81 0.10625733123554681 2316 82-83 0.1841627686276759 2316 84-85 0.09773430487242152 2316 86-87 0.26023184990969384 2316 88-89 0.37744495149429724 2316 90-91 0.6518396724044884 2316 92-93 0.6784484216190201 2316 94-95 0.8368219415873845 2316 96-97 1.1054260288001494 2316 98-99 1.373801518323205 2316 100-101 1.8274007818304234 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7245.0 3 1092.0 4 1116.0 5 1270.0 6 1478.0 7 1820.0 8 2323.0 9 3004.0 10 3845.0 11 5177.0 12 7533.0 13 9885.0 14 11371.0 15 14987.0 16 18775.0 17 24896.0 18 20753.0 19 14857.0 20 14731.0 21 15607.0 22 16852.0 23 19297.0 24 21661.0 25 24666.0 26 28729.0 27 33263.0 28 39419.0 29 47432.0 30 57602.0 31 70817.0 32 88938.0 33 113909.0 34 149186.0 35 202734.0 36 275708.0 37 341466.0 38 229552.0 39 20683.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18233834810791 19.21482198980697 15.188595577026037 22.414244085059078 2 14.72220491708684 20.539402771976302 40.98193650252207 23.75645580841479 3 18.56467519682024 25.203560482969138 30.765130061183037 25.466634259027586 4 11.956353564923536 16.86599028887755 38.858225680194735 32.31943046600418 5 12.72355622914054 37.71916476369831 35.19810615499626 14.359172852164889 6 30.38007119762906 36.805417308558106 18.552270636746105 14.262240857066727 7 26.968776144727514 33.250044536961084 21.89605456122288 17.885124757088523 8 25.699431348327224 33.92619779277394 21.00626844034262 19.368102418556216 9 26.018902398867876 16.333937274830138 21.26057854838603 36.38658177791596 10-11 23.856060240554804 26.72245422364699 28.646428041735238 20.775057494062967 12-13 25.18975125005456 24.340257844732108 28.80073636375471 21.66925454145862 14-15 22.165051727812298 25.508351613854146 27.899124704417062 24.4274719539165 16-17 20.82061830937928 28.676620902363677 28.52175644066953 21.981004347587515 18-19 20.651887388278226 27.170888861953035 31.31649558854489 20.86072816122385 20-21 21.91500557864253 26.06525021207008 31.20576769368812 20.813976515599272 22-23 21.215021862053938 26.16017916319397 31.238162413599333 21.386636561152763 24-25 20.987943228928085 26.20424162539922 31.442461423819303 21.365353721853392 26-27 20.805818704444082 26.504788738432627 31.251523155925486 21.437869401197805 28-29 20.70210526423256 27.016545226784768 31.10645055574443 21.174898953238245 30-31 21.06878054583132 26.473487762777747 31.101469977847973 21.356261713542963 32-33 20.736513106257746 26.45157914484843 31.07944922960295 21.732458519290876 34-35 21.27645726318476 26.572695657722615 31.01244935073222 21.1383977283604 36-37 21.14516275158579 26.428397341258346 30.939785294744404 21.48665461241146 38-39 20.895266455640485 26.77751282551809 30.88091310288866 21.44630761595276 40-41 21.36446923599613 26.647907147524784 30.668420140762738 21.319203475716346 42-43 20.961398643015347 26.717061273570952 30.493032993657586 21.828507089756112 44-45 21.255703567600673 26.80760986066452 30.314295922012963 21.62239064972184 46-47 21.41328557657632 27.084298383768623 29.729244811825055 21.773171227829998 48-49 21.326231931702395 27.129935537092205 29.65513263187126 21.888699899334135 50-51 21.458205376019638 27.434252277448728 29.474574722220947 21.63296762431069 52-53 21.492036445233982 27.55060852266185 29.12399238047774 21.833362651626427 54-55 21.43754125298821 27.334427797762984 29.286750191046323 21.941280758202485 56-57 21.544587551591935 27.584840423835317 28.932667342863887 21.937904681708865 58-59 21.67929173736111 27.415479212644634 29.049483983722208 21.855745066272053 60-61 21.419389802001266 27.35177395659143 29.508297106792558 21.720539134614747 62-63 21.162251772226252 27.320198964961694 29.660236759526846 21.857312503285208 64-65 21.177251920511285 27.47370196297953 29.306100246491873 22.042945870017316 66-67 21.17663388699267 27.46047213242917 29.1529106600153 22.20998332056286 68-69 21.351900832266356 27.350071877317134 29.266136929733733 22.031890360682777 70-71 21.388512879919674 27.18106394732444 29.1268497621256 22.303573410630285 72-73 21.234182948331604 27.087952886300947 29.362750476520976 22.315113688846473 74-75 21.170413700502333 27.35377228737903 29.095909538388366 22.379904473730274 76-77 21.241578443264757 27.132195631496486 29.171514562061184 22.45471136317757 78-79 21.181893258690774 27.246983979207883 29.051109532238602 22.52001322986274 80-81 21.331106444113843 27.221168887176333 28.943928834812965 22.503795833896863 82-83 21.234584723007906 27.207869800154782 29.25584888097547 22.301696595861838 84-85 21.17028384936239 27.206110656272013 29.053477427039997 22.570128067325598 86-87 21.152935995654477 27.295221226926213 29.02031592802742 22.531526849391888 88-89 21.306464498879684 27.03809579460417 28.734298565267725 22.92114114124842 90-91 21.169966045524564 26.797600757934724 29.03685592835649 22.99557726818422 92-93 21.255638999896146 26.542666295475303 29.291795964850458 22.909898739778097 94-95 21.002359718221882 26.57045779758272 29.283444821173777 23.143737663021625 96-97 20.7114539176128 26.454106447849373 29.578841696004147 23.255597938533683 98-99 20.791848015403637 26.76920446440078 29.209721440684127 23.229226079511452 100-101 20.954067345780206 26.002002895906823 29.744917811102678 23.299011947210296 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3030.0 1 1698.0 2 369.5 3 370.5 4 313.5 5 301.0 6 496.0 7 939.0 8 1561.5 9 1974.0 10 1999.0 11 1923.5 12 2043.5 13 2307.0 14 2525.5 15 2714.5 16 3001.0 17 3282.5 18 3504.0 19 3897.0 20 4435.5 21 4997.0 22 5708.0 23 6869.5 24 8210.5 25 10051.0 26 12779.5 27 15973.5 28 19261.5 29 23019.5 30 27438.0 31 32087.0 32 36744.5 33 40477.0 34 44538.5 35 49109.5 36 53661.0 37 57651.0 38 60407.0 39 62818.5 40 65330.0 41 67888.5 42 69135.5 43 70342.5 44 72312.0 45 74010.0 46 76042.5 47 79080.0 48 81862.5 49 80405.0 50 74188.0 51 67795.0 52 63209.0 53 59688.5 54 56766.5 55 51812.5 56 44977.0 57 38788.0 58 34381.5 59 29691.5 60 23546.0 61 18515.5 62 14418.5 63 10521.0 64 7288.0 65 5174.0 66 3735.0 67 2742.0 68 2038.5 69 1468.5 70 1067.0 71 692.5 72 478.0 73 380.5 74 276.5 75 189.5 76 144.0 77 99.5 78 60.5 79 37.0 80 25.5 81 21.5 82 19.0 83 15.5 84 9.5 85 6.0 86 6.0 87 6.0 88 4.5 89 2.5 90 3.0 91 5.0 92 2.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31894214889500777 2 0.27321166035793015 3 0.26954507330373245 4 0.27020709596629594 5 0.27168391575201445 6 0.2355272934120088 7 0.23588376715338913 8 0.23725873729871327 9 0.24851312256229252 10-11 0.25454771375565965 12-13 0.24772378784923607 14-15 0.23560368064230458 16-17 0.2315296950265293 18-19 0.21854386587624558 20-21 0.2251386300917818 22-23 0.22142111821738686 24-25 0.22440022019892253 26-27 0.2211410317063023 28-29 0.23858278262384025 30-31 0.2516704614145184 32-33 0.24375165187385514 34-35 0.24146003496498153 36-37 0.22821958171371187 38-39 0.2142152561594843 40-41 0.2108542180264697 42-43 0.22450206983931692 44-45 0.22299978764349979 46-47 0.23346483819402256 48-49 0.24051792579133352 50-51 0.25554074774950486 52-53 0.26931591161284507 54-55 0.24087439953271386 56-57 0.21345138385652643 58-59 0.2013567390596936 60-61 0.20471777719270814 62-63 0.2107269059759767 64-65 0.20265532197472194 66-67 0.20779872881463826 68-69 0.20828251460651157 70-71 0.21065051874568094 72-73 0.21205095130110369 74-75 0.214800891591752 76-77 0.22893252919647256 78-79 0.22788857038243013 80-81 0.21867117792673854 82-83 0.2107014435658781 84-85 0.19807208815697475 86-87 0.2016877503909753 88-89 0.21309491011514609 90-91 0.21100699248706128 92-93 0.21503005328263936 94-95 0.20996303367301888 96-97 0.2188239523873301 98-99 0.22414559609793658 100-101 0.20453954032201802 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1963679.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.20183890545626 #Duplication Level Percentage of deduplicated Percentage of total 1 76.04418381461696 45.77999703704474 2 13.85279480077459 16.67927441973148 3 4.690538633284424 8.471371535424238 4 2.0771968194899437 5.002042732074388 5 1.0501001880348568 3.1608981177331885 6 0.6076699173361708 2.1949707882698446 7 0.3832771671321499 1.6151793190280526 8 0.26375368830666124 1.2702765643326033 9 0.18019472852138085 0.9763248616250936 >10 0.7829984935673396 8.060667752262587 >50 0.04239061921141305 1.7160249006138932 >100 0.022361073529801635 2.6140308544751143 >500 0.0016933707961589084 0.6596798561637075 >1k 6.773483184635548E-4 0.9314626558132798 >5k 1.693370796158887E-4 0.8677986054077426 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9968 0.5076186077256007 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7057 0.35937645613157754 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4796 0.24423543766572847 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3293 0.16769543290935024 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2570 0.1308767879067811 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2038 0.10378478356187545 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.092482019719109E-5 0.0 10-11 0.0 0.0 0.0 5.092482019719109E-5 0.0 12-13 0.0 0.0 0.0 5.092482019719109E-5 0.0 14-15 0.0 0.0 0.0 5.092482019719109E-5 0.0 16-17 0.0 0.0 0.0 5.092482019719109E-5 0.0 18-19 0.0 0.0 0.0 5.092482019719109E-5 0.0 20-21 0.0 0.0 0.0 1.0184964039438218E-4 0.0 22-23 0.0 0.0 0.0 2.80086511084551E-4 0.0 24-25 0.0 0.0 0.0 5.092482019719109E-4 0.0 26-27 0.0 0.0 0.0 0.0010184964039438218 0.0 28-29 0.0 0.0 0.0 0.0020115303977890483 0.0 30-31 0.0 0.0 0.0 0.004023060795578097 0.0 32-33 0.0 0.0 0.0 0.006416527344846077 0.0 34-35 0.0 0.0 0.0 0.008071584001254788 0.0 36-37 0.0 0.0 0.0 0.01102522357269187 0.0 38-39 0.0 0.0 0.0 0.016143168002509575 0.0 40-41 0.0 0.0 0.0 0.02388374067248262 0.0 42-43 0.0 0.0 0.0 0.03277012179689247 0.0 44-45 0.0 0.0 0.0 0.03788806622671017 0.0 46-47 0.0 0.0 0.0 0.045348552385598664 0.0 48-49 0.0 0.0 0.0 0.054438632790797276 0.0 50-51 0.0 0.0 0.0 0.06531108190289757 0.0 52-53 0.0 0.0 0.0 0.09100265369238048 0.0 54-55 0.0 0.0 0.0 0.10798608122814371 0.0 56-57 0.0 0.0 0.0 0.16071873254233507 0.0 58-59 0.0 0.0 0.0 0.2595892709551816 0.0 60-61 0.0 0.0 0.0 0.3474600482054348 0.0 62-63 0.0 0.0 0.0 0.3926303637203433 0.0 64-65 0.0 0.0 0.0 0.47067265067253866 0.0 66-67 0.0 0.0 0.0 0.6411689486927343 0.0 68-69 0.0 0.0 0.0 0.8831382318596879 0.0 70-71 0.0 0.0 0.0 1.1040246394650044 0.0 72-73 0.0 0.0 0.0 1.3712017086295671 0.0 74-75 0.0 0.0 0.0 1.6947016289322236 0.0 76-77 0.0 0.0 0.0 1.928115542306049 0.0 78-79 0.0 0.0 0.0 2.2366944902909287 0.0 80-81 0.0 0.0 0.0 2.573765875176136 0.0 82-83 0.0 0.0 0.0 2.8235521182433585 0.0 84-85 0.0 0.0 0.0 3.1885048421865285 0.0 86-87 0.0 0.0 0.0 3.6831630831719444 0.0 88-89 0.0 0.0 0.0 4.367745441082784 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11425 0.0 34.447624 1 GGTATCA 7235 0.0 33.63695 1 TATCAAC 13960 0.0 28.043184 2 ATCAACG 13905 0.0 28.017437 3 TCAACGC 13985 0.0 27.825327 4 CAACGCA 14090 0.0 27.618675 5 AACGCAG 14500 0.0 26.928476 6 TACATGG 8215 0.0 26.834654 2 GTACATG 8285 0.0 26.562788 1 ACATGGG 8235 0.0 25.038694 3 ACGCAGA 16170 0.0 24.031698 7 CGCAGAG 16075 0.0 23.937305 8 CATGGGT 2825 0.0 23.378424 4 TAGGACG 205 2.6635462E-8 23.177414 4 GAGTACT 8755 0.0 22.35425 12-13 AGAGTAC 12960 0.0 22.303015 10-11 GCAGAGT 16850 0.0 22.13094 9 AGTACTT 8950 0.0 21.946815 12-13 TAGCCCT 1515 0.0 21.639902 4 GTACTTT 9390 0.0 20.713955 14-15 >>END_MODULE