FastQCFastQC Report
Thu 26 May 2016
SRR937439_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937439_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963679
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106930.5445391023685643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82690.42109733821057316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55650.2833966243973684No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37260.189745880054734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98100.029.4988441
GTATCAA141200.029.1097261
GTACATG95400.025.9505211
CGTACAC1109.48368E-525.9076043
TACATGG92600.025.4925982
TCAACGC172500.023.8725414
ATCAACG172200.023.8589693
TATCAAC172400.023.858842
ACATGGG95750.023.8106463
CAACGCA174200.023.6395745
AACGCAG178600.023.0837826
GAGTACT100750.022.46797812-13
AGTACTT102400.021.71160712-13
CATGGGT29950.021.5680454
GTACTTT105400.021.2926614-15
AGAGTAC148750.021.1739910-11
ACGCAGA198900.020.6598637
CGCAGAG198050.020.6285978
TAGCCCT13850.020.5764374
GTACCGT1856.5592485E-620.5393626
Produced by FastQC (version 0.11.5)