##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937439_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1963679 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2788077888494 33.0 31.0 34.0 31.0 34.0 2 32.4843755012912 34.0 31.0 34.0 31.0 34.0 3 32.54785278041879 34.0 31.0 34.0 31.0 34.0 4 36.161870142727 37.0 35.0 37.0 35.0 37.0 5 36.09949131197105 37.0 35.0 37.0 35.0 37.0 6 36.1389891117642 37.0 35.0 37.0 35.0 37.0 7 36.082593438133216 37.0 35.0 37.0 35.0 37.0 8 36.06013966641187 37.0 35.0 37.0 35.0 37.0 9 37.76507463796272 39.0 37.0 39.0 35.0 39.0 10-11 37.68734044617272 39.0 37.0 39.0 35.0 39.0 12-13 37.59342081877945 39.0 37.0 39.0 35.0 39.0 14-15 38.83942232920961 40.0 38.0 41.0 35.0 41.0 16-17 38.80813590204916 40.0 38.0 41.0 35.0 41.0 18-19 38.81705742129951 40.0 38.0 41.0 35.0 41.0 20-21 38.72145931183253 40.0 38.0 41.0 34.5 41.0 22-23 38.65969005117435 40.0 38.0 41.0 34.0 41.0 24-25 38.684636083596146 40.0 38.0 41.0 34.0 41.0 26-27 38.53737983652114 40.0 38.0 41.0 34.0 41.0 28-29 38.396178295943486 40.0 38.0 41.0 34.0 41.0 30-31 38.41041178318859 40.0 38.0 41.0 34.0 41.0 32-33 38.28207767155426 40.0 38.0 41.0 34.0 41.0 34-35 38.14910609116867 40.0 38.0 41.0 34.0 41.0 36-37 37.980558940641515 40.0 37.5 41.0 33.5 41.0 38-39 37.858325113218605 40.0 37.0 41.0 33.0 41.0 40-41 37.65888798525624 40.0 37.0 41.0 33.0 41.0 42-43 37.44379809530987 40.0 37.0 41.0 33.0 41.0 44-45 37.116433745026555 40.0 37.0 41.0 32.0 41.0 46-47 36.718960940153664 40.0 36.0 41.0 31.0 41.0 48-49 36.352190200129456 39.5 36.0 41.0 30.0 41.0 50-51 36.090900549427886 39.0 36.0 41.0 30.0 41.0 52-53 35.859388423464324 39.0 35.0 41.0 29.0 41.0 54-55 35.65788909490808 39.0 35.0 40.0 28.0 41.0 56-57 35.35639659027774 39.0 35.0 40.0 28.0 41.0 58-59 35.099632373723 38.0 35.0 40.0 27.0 41.0 60-61 34.760808411150705 38.0 34.0 40.0 26.0 41.0 62-63 34.400096706233555 38.0 34.0 40.0 26.0 41.0 64-65 33.99913580580125 37.0 34.0 40.0 25.5 41.0 66-67 33.572946240195066 36.5 33.0 39.0 24.5 40.5 68-69 33.12994690068999 36.0 33.0 39.0 23.0 40.0 70-71 32.69264681243727 35.5 32.0 38.5 22.0 40.0 72-73 32.22319839444227 35.0 32.0 38.0 20.5 40.0 74-75 31.755581487605664 35.0 31.0 37.0 19.5 39.0 76-77 30.43458248522289 33.5 29.5 35.5 18.5 38.5 78-79 31.060487992182022 34.0 31.0 36.0 18.0 38.5 80-81 30.879630275620407 34.0 31.0 36.0 18.0 37.5 82-83 30.49211862020218 34.0 31.0 35.0 14.0 37.0 84-85 30.07061642967104 34.0 30.0 35.0 8.5 36.5 86-87 29.72985197682513 34.0 30.0 35.0 6.0 36.0 88-89 29.29940153151304 34.0 29.5 35.0 2.0 36.0 90-91 28.790041804184902 34.0 29.0 35.0 2.0 35.0 92-93 28.349101864408592 34.0 29.0 35.0 2.0 35.0 94-95 27.914811942277737 33.0 28.5 35.0 2.0 35.0 96-97 27.3124064574709 33.0 27.0 35.0 2.0 35.0 98-99 26.442593977936312 33.0 25.5 34.5 2.0 35.0 100-101 23.595256149299352 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2650263758587954 1101 2 0.2762548236461555 1101 3 0.2813692163277679 1101 4 0.16903284132388308 1101 5 0.1618699587389827 1101 6 0.15627777732321846 1101 7 0.18096705089031673 1101 8 0.17151708504224672 1101 9 0.3228243209355881 1101 10-11 0.25189372695773216 1101 12-13 0.22116905694166888 1101 14-15 0.3328031192177505 1101 16-17 0.2739532297364349 1101 18-19 0.22609731292975965 1101 20-21 0.3928527033721636 1101 22-23 0.41234781680748966 1101 24-25 0.3201483514147867 1101 26-27 0.2987846612942917 1101 28-29 0.12949815134288656 1101 30-31 0.3324749069597033 1101 32-33 0.2764087771588919 1101 34-35 0.3178514347747097 1101 36-37 0.3638481712234878 1101 38-39 0.2325742619797211 1101 40-41 0.3315148054878847 1101 42-43 0.31895466595589284 1101 44-45 0.3156944531526804 1101 46-47 0.11686904973394263 1101 48-49 0.16457094945919692 1101 50-51 -0.016704616806649142 1101 52-53 0.029286691708115598 1101 54-55 -0.0840084784517714 1101 56-57 0.08613711939970159 1101 58-59 0.2133593717801645 1101 60-61 0.22819157536983425 1101 62-63 0.2724485539627395 1101 64-65 0.2646531784346067 1101 66-67 0.24743610317573683 1101 68-69 0.38987199201233835 1101 70-71 0.28705427886374935 1101 72-73 0.30333517297703594 1101 74-75 0.36562814380616615 1101 76-77 0.3467950744229782 1101 78-79 0.3446018642863322 1101 80-81 0.22681878235100683 1101 82-83 0.26347714791644705 1101 84-85 0.39300774032439634 1101 86-87 0.371543128546886 1101 88-89 0.5565715038643582 1101 90-91 0.6040118464724031 1101 92-93 0.525169029593112 1101 94-95 0.657925193478949 1101 96-97 0.7823041295399733 1101 98-99 1.1513488213140484 1101 100-101 1.8804573831564007 1102 1 0.2454232391498934 1102 2 0.13013760293138432 1102 3 0.19715998623271247 1102 4 0.1641906623282381 1102 5 0.11341236948118905 1102 6 0.13351761668356232 1102 7 0.16815413588260242 1102 8 0.15137803710525333 1102 9 0.18777253487393608 1102 10-11 0.23026113648146662 1102 12-13 0.13917625648308984 1102 14-15 0.2745371683645814 1102 16-17 0.28672085647134793 1102 18-19 0.311277545159399 1102 20-21 0.3081918844998057 1102 22-23 0.30650696669916755 1102 24-25 0.15307527641439123 1102 26-27 0.23431119784990528 1102 28-29 0.209212843850608 1102 30-31 0.23530913333465264 1102 32-33 0.2696614714876304 1102 34-35 0.28171850981220103 1102 36-37 0.35823512112732203 1102 38-39 0.3176047194214746 1102 40-41 0.29739861044466664 1102 42-43 0.3442184054473998 1102 44-45 0.24165707695680538 1102 46-47 0.10092725422213533 1102 48-49 0.27179104313260183 1102 50-51 0.40384379398473413 1102 52-53 0.46546785946407 1102 54-55 0.47310812073536823 1102 56-57 0.5192404098730847 1102 58-59 0.46327720591380483 1102 60-61 0.4891047551629839 1102 62-63 0.47623122459924616 1102 64-65 0.5462004501180857 1102 66-67 0.4342579921625358 1102 68-69 0.3949449255407984 1102 70-71 0.376406315822436 1102 72-73 0.4982636627942867 1102 74-75 0.5100555727995477 1102 76-77 0.37108824309677857 1102 78-79 0.3865644682498761 1102 80-81 0.4006127448635297 1102 82-83 0.3958622095149664 1102 84-85 0.552965704180906 1102 86-87 0.6659694145505277 1102 88-89 0.58723371330235 1102 90-91 0.8238607475416799 1102 92-93 0.8898418477011738 1102 94-95 0.7866587263394713 1102 96-97 0.8656166518336654 1102 98-99 1.1334218898446942 1102 100-101 1.9428283924508527 1103 1 0.22373596557635267 1103 2 0.19861908698816677 1103 3 0.15232263960197656 1103 4 0.11040481428058513 1103 5 0.10062582279009291 1103 6 0.06819563760691238 1103 7 0.15776885081763226 1103 8 0.1688330072414388 1103 9 0.23556196932278084 1103 10-11 0.21495956801038574 1103 12-13 0.11070156965462274 1103 14-15 0.23096005246119944 1103 16-17 0.2336969176454602 1103 18-19 0.1880139248051762 1103 20-21 0.34034603825907084 1103 22-23 0.31115114811562705 1103 24-25 0.2792844806278154 1103 26-27 0.228412580142205 1103 28-29 0.30442997437962305 1103 30-31 0.3066897275079583 1103 32-33 0.1885582865344304 1103 34-35 0.19408215727019495 1103 36-37 0.11865801090011985 1103 38-39 0.20588468008552496 1103 40-41 0.29841464707874366 1103 42-43 0.3037719405973931 1103 44-45 0.16792938139694513 1103 46-47 0.3236533164605291 1103 48-49 0.44646916811929316 1103 50-51 0.33850360382315614 1103 52-53 0.39448495281408924 1103 54-55 0.4318005317269211 1103 56-57 0.45639490183820897 1103 58-59 0.321544352596284 1103 60-61 0.3776198043778507 1103 62-63 0.4545018526735447 1103 64-65 0.6334697428705027 1103 66-67 0.5815330462399899 1103 68-69 0.696982509435955 1103 70-71 0.5140488885142247 1103 72-73 0.39886129383959457 1103 74-75 0.489364823983383 1103 76-77 0.5585299735377198 1103 78-79 0.6313896820409184 1103 80-81 0.713176454097308 1103 82-83 0.6681237235325383 1103 84-85 0.7392343569437649 1103 86-87 0.800387324419372 1103 88-89 0.9447862522823449 1103 90-91 1.1563187662607852 1103 92-93 1.1298002081737515 1103 94-95 0.9629342236321712 1103 96-97 1.1004699958382247 1103 98-99 1.5499815368239958 1103 100-101 2.169961182478932 1104 1 0.24196693431281346 1104 2 0.2866726125047734 1104 3 0.27378319871127843 1104 4 0.1410973154198416 1104 5 0.20341799368468116 1104 6 0.20995189790947677 1104 7 0.25041598410258104 1104 8 0.24237292633993235 1104 9 0.30770102089608287 1104 10-11 0.25641455740542796 1104 12-13 0.28822355314801484 1104 14-15 0.30615330466797985 1104 16-17 0.3350966599758536 1104 18-19 0.36516318752128285 1104 20-21 0.40602787817760344 1104 22-23 0.2503456211587718 1104 24-25 0.17231783702764147 1104 26-27 0.31066642971491376 1104 28-29 0.23728116174143565 1104 30-31 0.2538400310292559 1104 32-33 0.24641931935073558 1104 34-35 0.2726854529657601 1104 36-37 0.26310419001988095 1104 38-39 0.3528892708803042 1104 40-41 0.23806763700262934 1104 42-43 0.16811282961380414 1104 44-45 0.18903873907949986 1104 46-47 0.25373863435618915 1104 48-49 0.24256136658114968 1104 50-51 0.20238316297184156 1104 52-53 0.19398086554398475 1104 54-55 0.26099244495190277 1104 56-57 0.24166202308357043 1104 58-59 0.27601615802760193 1104 60-61 0.3946447049387629 1104 62-63 0.334909116931172 1104 64-65 0.32966785029284296 1104 66-67 0.5052061602728628 1104 68-69 0.4729013716389687 1104 70-71 0.37577862234940085 1104 72-73 0.4627723584426562 1104 74-75 0.3328171747966202 1104 76-77 0.4164241996006055 1104 78-79 0.28114607736685926 1104 80-81 0.393682313549224 1104 82-83 0.12973559321247308 1104 84-85 0.25472547634381115 1104 86-87 0.4919447065411511 1104 88-89 0.6447636077370085 1104 90-91 0.6289828618573345 1104 92-93 0.5591175806188566 1104 94-95 0.7290849884833328 1104 96-97 1.0073228541143102 1104 98-99 1.2247299151369937 1104 100-101 1.9206004337743146 1105 1 0.17213014424266504 1105 2 0.14661509675683249 1105 3 0.1613172702540524 1105 4 0.10295824911614204 1105 5 0.13007181276896063 1105 6 0.12257298023053664 1105 7 0.08407533361474151 1105 8 0.15105066088290897 1105 9 0.19267742311668457 1105 10-11 0.16626384403113548 1105 12-13 0.16152173114319623 1105 14-15 0.22250382778573652 1105 16-17 0.19171667517530722 1105 18-19 0.2981512859562301 1105 20-21 0.39581870128289154 1105 22-23 0.320878760725293 1105 24-25 0.16230611273114448 1105 26-27 0.17571262484722183 1105 28-29 0.3385462624773652 1105 30-31 0.23678166228609143 1105 32-33 0.23336177903091482 1105 34-35 0.20586705494695678 1105 36-37 0.2552510614053958 1105 38-39 0.16035390053821885 1105 40-41 0.09039678785114802 1105 42-43 -0.03746165213893704 1105 44-45 0.0018056291747470254 1105 46-47 -0.017237632719066198 1105 48-49 0.060805811142607524 1105 50-51 -0.03095798070871325 1105 52-53 -0.08791825987264446 1105 54-55 -0.19257789292898764 1105 56-57 0.028012388239247343 1105 58-59 0.01742565365469062 1105 60-61 0.1013821824949801 1105 62-63 0.11621632333671528 1105 64-65 0.09175787288142345 1105 66-67 0.1335913753733351 1105 68-69 0.11084965485444798 1105 70-71 0.17138468774219007 1105 72-73 0.149775326374602 1105 74-75 0.19189635241093583 1105 76-77 0.11788442738192373 1105 78-79 0.10525267017747808 1105 80-81 0.0883751826144561 1105 82-83 0.12595744744155724 1105 84-85 0.09934300627323722 1105 86-87 0.3607493021845585 1105 88-89 0.5086206994445277 1105 90-91 0.6207054295484724 1105 92-93 0.6034380102055046 1105 94-95 0.791598919861098 1105 96-97 1.0917143139822905 1105 98-99 1.220196842265576 1105 100-101 1.7038586912419724 1106 1 0.030544666883955074 1106 2 0.1984282101753294 1106 3 0.07614217100915965 1106 4 0.12046165377297058 1106 5 0.08804522958396888 1106 6 0.08408327609013355 1106 7 0.03860236666820782 1106 8 0.04238199514848873 1106 9 0.037122812212494694 1106 10-11 -0.060222377996829835 1106 12-13 -0.14489321085375906 1106 14-15 -0.0030380616201597377 1106 16-17 -0.014080551757125193 1106 18-19 0.10830000595652223 1106 20-21 0.21767104323301112 1106 22-23 0.2829096000573017 1106 24-25 0.21452541174998885 1106 26-27 0.19395485698770187 1106 28-29 0.1546990967531201 1106 30-31 0.19338135027295067 1106 32-33 0.05644310951500131 1106 34-35 -0.23295343944415947 1106 36-37 0.19997278710611255 1106 38-39 0.245348530803696 1106 40-41 0.1444589828859506 1106 42-43 0.02293795103793883 1106 44-45 0.02312939521809909 1106 46-47 0.14336724088222752 1106 48-49 0.25951076365996784 1106 50-51 0.1933452708102763 1106 52-53 0.06750112969364608 1106 54-55 0.002746656537503611 1106 56-57 0.17045809237339427 1106 58-59 0.1578196967179224 1106 60-61 0.04091672900407417 1106 62-63 0.1398867076991337 1106 64-65 0.12392659885910007 1106 66-67 0.09658793237988306 1106 68-69 0.031576047712462696 1106 70-71 0.08416992391923372 1106 72-73 0.2674150216219573 1106 74-75 0.33758306556488193 1106 76-77 4.568875027111119E-4 1106 78-79 0.10050623170149109 1106 80-81 0.01177327266847783 1106 82-83 -0.06315474428117795 1106 84-85 0.032756081511589485 1106 86-87 0.19549591800000243 1106 88-89 0.2983164423064828 1106 90-91 0.30620889366618087 1106 92-93 0.24875087613113678 1106 94-95 -0.14182928138997752 1106 96-97 0.29773895269226003 1106 98-99 0.5790647540986562 1106 100-101 0.8892531437507252 1107 1 0.10047955367174666 1107 2 0.09761571077106623 1107 3 0.16090092467958783 1107 4 -0.0249071108365726 1107 5 0.014082328359478424 1107 6 0.029320165839493484 1107 7 0.05016608740076833 1107 8 0.017590418543043995 1107 9 0.10210650447309177 1107 10-11 0.05130257085400558 1107 12-13 0.10947948925596762 1107 14-15 0.08961271838711582 1107 16-17 0.17521229733979027 1107 18-19 0.1338743091980561 1107 20-21 0.12772700594472042 1107 22-23 0.20106672738327092 1107 24-25 0.059980354786439705 1107 26-27 0.030724814414327284 1107 28-29 0.12631768684713762 1107 30-31 0.11870973381348904 1107 32-33 0.09989509097402305 1107 34-35 0.11546420240681954 1107 36-37 0.2326390085829857 1107 38-39 0.16031952338068578 1107 40-41 0.11919123823201971 1107 42-43 0.04602852697559712 1107 44-45 0.12466911270761472 1107 46-47 -0.02352005825671455 1107 48-49 -0.02004950208581846 1107 50-51 0.10172686574475165 1107 52-53 0.06626185924420724 1107 54-55 0.1507541799085672 1107 56-57 0.14933821016365556 1107 58-59 0.19091260513609853 1107 60-61 0.19337951444558854 1107 62-63 -0.008137075752486567 1107 64-65 0.005296695673862928 1107 66-67 0.05285280124233793 1107 68-69 0.1346847228018575 1107 70-71 0.20683302794617475 1107 72-73 0.1503986510849984 1107 74-75 0.10288744586237542 1107 76-77 0.07685123146172756 1107 78-79 0.18778291491419097 1107 80-81 0.11753584521506966 1107 82-83 0.1448942479022115 1107 84-85 0.16549707132437064 1107 86-87 0.29410321406635376 1107 88-89 0.49292872068579285 1107 90-91 0.7349321297137692 1107 92-93 0.6305723303542443 1107 94-95 0.5048798184799956 1107 96-97 0.5567283942930601 1107 98-99 0.9852564245640139 1107 100-101 1.7335705820142842 1108 1 0.11831032542692554 1108 2 0.13372588858391765 1108 3 0.14222302197007508 1108 4 0.09350955517895443 1108 5 0.06303751181118855 1108 6 0.1015997750523212 1108 7 0.15255368139258252 1108 8 0.16477698008993968 1108 9 0.12707505449223078 1108 10-11 0.20412343228395713 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-0.03993288761724045 2316 62-63 -0.040269019314607135 2316 64-65 0.11825755012579009 2316 66-67 0.08654481673636383 2316 68-69 0.1579909187837032 2316 70-71 0.09701186406980611 2316 72-73 0.2514054696296171 2316 74-75 0.08619577569358938 2316 76-77 0.02029933657091121 2316 78-79 0.07201262787073048 2316 80-81 0.14018844802263075 2316 82-83 0.22383119998344725 2316 84-85 0.20543263233128428 2316 86-87 0.24529711093138573 2316 88-89 0.43210261149134155 2316 90-91 0.4027430934643945 2316 92-93 0.38153744924712285 2316 94-95 0.7113297446911595 2316 96-97 0.7096826354408421 2316 98-99 1.0214854403544997 2316 100-101 1.6121635119686424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 3.0 4 8.0 5 26.0 6 60.0 7 187.0 8 414.0 9 885.0 10 1538.0 11 2627.0 12 4287.0 13 6279.0 14 8000.0 15 11462.0 16 15935.0 17 23127.0 18 20506.0 19 11989.0 20 9863.0 21 9592.0 22 9882.0 23 10731.0 24 12284.0 25 14179.0 26 17125.0 27 21393.0 28 26493.0 29 32762.0 30 41948.0 31 53612.0 32 69693.0 33 92189.0 34 128066.0 35 186462.0 36 290676.0 37 442919.0 38 355817.0 39 30656.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.077948258654764 20.12268713456102 15.251424056195006 21.54794055058921 2 15.624702408081973 21.84379422502354 39.40465829700272 23.12684506989177 3 19.295618071996493 25.73093667549533 30.484666791262725 24.48877846124545 4 12.792314833534402 17.75519318585166 37.756425566500425 31.69606641411351 5 13.02194122694669 38.642225849245406 34.236767660306924 14.09906526350097 6 30.599298561526606 37.74456008339448 17.631853271334062 14.024288083744848 7 27.520129308303442 34.01976595971134 20.982044417646673 17.478060314338546 8 26.4368565330688 34.700376181646796 20.04798136558979 18.814785919694614 9 26.599968732160402 16.710826973247663 20.675426075239383 36.01377821935255 10-11 24.33032588320189 27.56155664953386 27.570188406557282 20.53792906070697 12-13 25.881082143681986 24.959849824210092 28.021270489830876 21.137797542277045 14-15 22.55088535346154 26.327928342667008 27.147206849999417 23.973979453872044 16-17 21.13698318309663 29.4227569781008 27.79738949186705 21.64287034693552 18-19 21.082366313435138 27.79000539293846 30.700231555157437 20.427396738468968 20-21 22.308735092380264 26.528395454038524 30.843820037613114 20.319049415968106 22-23 21.764125785932915 26.634166289184613 30.76783032990022 20.83387759498225 24-25 21.553069914448827 26.769351500258313 30.899711943681208 20.77786664161165 26-27 21.224997701977113 27.02189729352742 30.845221729289594 20.907883275205876 28-29 21.11750663636537 27.614053180044433 30.562700125406618 20.70574005818358 30-31 21.48236267082757 27.060726922363976 30.624985995653052 20.831924411155406 32-33 21.33819428459685 27.07027534073849 30.53469615150361 21.05683422316105 34-35 21.693591467851924 27.16467406332705 30.483826531729473 20.65790793709155 36-37 21.781136779773266 27.007705181881864 30.367827523701052 20.843330514643817 38-39 21.435204388091027 27.400335645575385 30.352957472417202 20.811502493916393 40-41 21.847027951106064 27.219112696117847 30.128600448443965 20.805258904332124 42-43 21.51618137481589 27.365403067595896 29.888382534790058 21.23003302279816 44-45 21.76692320893588 27.467243882528663 29.69057060751783 21.07526230101763 46-47 22.063789448275408 27.728768296651335 29.130295735708334 21.077146519364927 48-49 21.98864987607445 27.874031346263823 28.839820561303554 21.297498216358175 50-51 22.01642936549202 28.25571287364177 28.68556928092626 21.04228847993995 52-53 22.234540370396587 28.336963424266386 28.26941165027482 21.159084555062208 54-55 22.082911718259453 28.265795988040814 28.29889712116899 21.352395172530745 56-57 22.10180482655261 28.438889451891068 28.17033741258118 21.288968308975143 58-59 22.242357330296855 28.183705177882945 28.156358549437048 21.41757894238315 60-61 21.922778621149384 28.30796173916409 28.68378691221936 21.085472727467167 62-63 21.832030591557988 28.197582191386676 28.76333148136737 21.20705573568796 64-65 21.850185289958286 28.41526033531957 28.431301653681686 21.303252721040455 66-67 21.804964049623184 28.335053743508997 28.278298031399224 21.581684175468595 68-69 21.91778798877006 28.276159188950945 28.351960783814462 21.454092038464534 70-71 22.09431887798362 28.095579776531704 28.115364069178312 21.694737276306363 72-73 21.912921524262902 28.06181973919336 28.31835378221571 21.706904954328028 74-75 21.799133157710603 28.50613567696146 27.951437072963554 21.743294092364383 76-77 21.84562751855064 28.075439010143715 28.283925224031016 21.79500824727463 78-79 21.764707979257302 28.284230772952196 28.021178614223608 21.92988263356689 80-81 21.987453142799815 28.334850044228205 27.9433654889623 21.734331324009677 82-83 21.79467854101907 28.363214426849293 28.204328421447165 21.63777861068447 84-85 21.605881010920335 28.462842138927197 27.988400326734148 21.942876523418324 86-87 21.538041604559606 28.46404631306848 27.923784895596484 22.074127186775435 88-89 21.711848015892617 28.389161364968512 27.597178561261792 22.301812057877076 90-91 21.67942718002077 28.078782609847114 27.816671326762343 22.425118883369773 92-93 21.539027204193008 27.94378528347345 28.034509213584197 22.482678298749345 94-95 21.434205896177534 28.02604193455244 28.00574839370386 22.53400377556617 96-97 21.113939701957396 28.054432521812373 28.263453446311743 22.568174329918484 98-99 21.076723137429845 28.35872200587573 27.73084187727985 22.833712979414575 100-101 20.930843146387655 28.609103482277163 27.870899329153026 22.589154042182148 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 371.0 1 273.0 2 214.0 3 276.0 4 265.5 5 283.5 6 477.0 7 897.0 8 1502.5 9 1955.5 10 1989.5 11 1982.5 12 2194.5 13 2442.5 14 2719.0 15 2946.0 16 3200.5 17 3526.5 18 3861.0 19 4217.0 20 4625.0 21 5254.0 22 6043.5 23 7060.5 24 8349.5 25 10243.0 26 12801.0 27 15991.5 28 19316.0 29 23050.0 30 27507.0 31 31662.5 32 35815.5 33 39671.0 34 43802.5 35 48284.5 36 53375.0 37 57662.5 38 60112.5 39 62411.5 40 65183.0 41 68178.5 42 69257.5 43 70524.5 44 72642.0 45 74213.5 46 75919.0 47 78961.0 48 82328.0 49 80232.0 50 74044.5 51 68483.5 52 63819.0 53 60379.0 54 57730.5 55 52705.5 56 45388.5 57 38902.0 58 34680.5 59 30116.0 60 23651.5 61 18420.5 62 14445.0 63 10400.0 64 6939.5 65 4726.5 66 3394.0 67 2608.0 68 1957.0 69 1489.0 70 1138.0 71 705.0 72 484.0 73 405.5 74 282.5 75 186.0 76 130.0 77 73.5 78 46.5 79 26.5 80 14.0 81 6.5 82 6.0 83 5.0 84 1.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04073985615775287 2 0.0 3 0.0 4 0.0 5 0.001222195684732586 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005779967092381189 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.0184964039438217E-4 22-23 4.837857918733153E-4 24-25 2.5462410098595542E-5 26-27 0.0017569062968030927 28-29 0.0033865005431132074 30-31 1.5277446059157326E-4 32-33 1.5277446059157326E-4 34-35 0.0 36-37 2.5462410098595542E-5 38-39 2.5462410098595542E-5 40-41 0.0 42-43 0.009522941376874735 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.0184964039438217E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.564737413803376E-4 84-85 1.5277446059157326E-4 86-87 0.0 88-89 0.0 90-91 0.010184964039438218 92-93 0.0015022821958171372 94-95 0.0 96-97 0.0 98-99 4.073985615775287E-4 100-101 0.013520539762354233 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1963679.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.257512368949584 #Duplication Level Percentage of deduplicated Percentage of total 1 74.81423517226503 43.58491230921733 2 14.341940073391953 16.71051502440731 3 4.8835180907956754 8.535048467355544 4 2.2538751805462365 5.252206448349633 5 1.151019091575614 3.3527754482181726 6 0.6879259324895179 2.404611211255756 7 0.4280461192844528 1.7455831462086415 8 0.2892875519068907 1.3482538506719064 9 0.19660509406875232 1.030835132955795 >10 0.8821402639089742 8.808518042687611 >50 0.04395951603307312 1.7841095572857597 >100 0.02472173330466218 2.830597856258287 >500 0.0017588260833459796 0.6910740614834966 >1k 7.035304333383874E-4 0.6643819726679472 >5k 1.7588260833459684E-4 0.7087492450561677 >10k+ 8.794130416729842E-5 0.5478282259206014 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10693 0.5445391023685643 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8269 0.42109733821057316 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5565 0.2833966243973684 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3726 0.189745880054734 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.092482019719109E-5 0.0 22-23 0.0 0.0 0.0 2.2916169088735988E-4 0.0 24-25 0.0 0.0 0.0 4.583233817747198E-4 0.0 26-27 0.0 0.0 0.0 9.421091736480352E-4 0.0 28-29 0.0 0.0 0.0 0.002138842448282026 0.0 30-31 0.0 0.0 0.0 0.004277684896564052 0.0 32-33 0.0 0.0 0.0 0.006722076266029223 0.0 34-35 0.0 0.0 0.0 0.008351670512339338 0.0 36-37 0.0 0.0 0.0 0.011381697314072209 0.0 38-39 0.0 0.0 0.0 0.016550566564087106 0.0 40-41 0.0 0.0 0.0 0.024443913694651723 0.0 42-43 0.0 0.0 0.0 0.03335575722916016 0.0 44-45 0.0 0.0 0.0 0.038244539968090505 0.0 46-47 0.0 0.0 0.0 0.045577714076486024 0.0 48-49 0.0 0.0 0.0 0.05451502002109306 0.0 50-51 0.0 0.0 0.0 0.06523469467260179 0.0 52-53 0.0 0.0 0.0 0.09092626646208468 0.0 54-55 0.0 0.0 0.0 0.10806246845843949 0.0 56-57 0.0 0.0 0.0 0.16148260484529295 0.0 58-59 0.0 0.0 0.0 0.26088785387020996 0.0 60-61 0.0 0.0 0.0 0.34952250342342106 0.0 62-63 0.0 0.0 0.0 0.39459096929793513 0.0 64-65 0.0 0.0 0.0 0.47242955696934175 0.0 66-67 0.0 0.0 0.0 0.644402674775256 0.0 68-69 0.0 0.0 0.0 0.888485337980393 0.0 70-71 0.0 0.0 0.0 1.1119943738258646 0.0 72-73 0.0 0.0 0.0 1.3834236654768932 0.0 74-75 0.0 0.0 0.0 1.7120669926194658 0.0 76-77 0.0 0.0 0.0 1.9496567412494608 0.0 78-79 0.0 0.0 0.0 2.2634554833045524 0.0 80-81 0.0 0.0 0.0 2.6058485119003665 0.0 82-83 0.0 0.0 0.0 2.8596323533530685 0.0 84-85 0.0 0.0 0.0 3.2320710258652254 0.0 86-87 0.0 0.0 0.0 3.740020644922108 0.0 88-89 0.0 0.0 0.0 4.44413267137857 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9810 0.0 29.498844 1 GTATCAA 14120 0.0 29.109726 1 GTACATG 9540 0.0 25.950521 1 CGTACAC 110 9.48368E-5 25.907604 3 TACATGG 9260 0.0 25.492598 2 TCAACGC 17250 0.0 23.872541 4 ATCAACG 17220 0.0 23.858969 3 TATCAAC 17240 0.0 23.85884 2 ACATGGG 9575 0.0 23.810646 3 CAACGCA 17420 0.0 23.639574 5 AACGCAG 17860 0.0 23.083782 6 GAGTACT 10075 0.0 22.467978 12-13 AGTACTT 10240 0.0 21.711607 12-13 CATGGGT 2995 0.0 21.568045 4 GTACTTT 10540 0.0 21.29266 14-15 AGAGTAC 14875 0.0 21.17399 10-11 ACGCAGA 19890 0.0 20.659863 7 CGCAGAG 19805 0.0 20.628597 8 TAGCCCT 1385 0.0 20.576437 4 GTACCGT 185 6.5592485E-6 20.539362 6 >>END_MODULE