FastQCFastQC Report
Thu 26 May 2016
SRR937438_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937438_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963878
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100250.5104695912882572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70900.36102038925024876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49300.25103392369587113No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27070.13783951956282417No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25890.1318309996853165No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19820.10092276607813724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA111550.035.8173451
GGTATCA69650.033.530811
ATCAACG135550.029.1427143
TATCAAC136700.029.038132
TCAACGC137600.028.679124
CAACGCA138700.028.3831125
AACGCAG142650.027.5364156
GTACATG88000.026.3767221
TACATGG85200.026.28042
ACATGGG86300.024.9526043
ACGCAGA159900.024.3275287
CGCAGAG159050.024.2485038
TGTATCG1452.3183435E-522.9528265
GAGTACT82950.022.8178412-13
GCAGAGT167200.022.612029
AGAGTAC128050.022.14406410-11
CATGGGT26950.021.699644
AGTACTT86700.021.42003612-13
GTATAGC11400.021.2788941
GTACTTT90600.020.57530614-15
Produced by FastQC (version 0.11.5)