FastQCFastQC Report
Thu 26 May 2016
SRR937438_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937438_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963878
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111450.5674996104646012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85660.4361778073790734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58520.2979818501963971No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38220.19461494043927371No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20450.10413070465680659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA146900.030.7040841
GGTATCA98250.028.1541331
ATCAACG178100.025.2050383
TCAACGC178800.025.106364
TATCAAC180050.024.958442
CAACGCA181000.024.80125
AACGCAG184200.024.3930246
TATACCG1201.7107275E-423.7514135
AGTACTT102500.023.4495812-13
GTACATG89600.023.3397621
TACATGG87500.023.127092
GAGTACT99800.022.6798812-13
AGAGTAC149800.022.65681310-11
CGCAGAG205200.021.6905528
ACGCAGA206300.021.6900277
ACATGGG89350.021.638113
GTACTTT106150.021.2762314-15
TACTTTT116700.020.53309814-15
CCTATAC4200.020.3583553
GCAGAGT213000.020.338729
Produced by FastQC (version 0.11.5)