FastQCFastQC Report
Thu 26 May 2016
SRR937437_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937437_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2153847
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105020.48759266558859565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73180.3397641522355116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52780.24504990373039495No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34210.15883208045882552No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29280.13594280373675569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA131850.032.573621
GGTATCA87600.030.7848741
TATCAAC159250.026.8993552
GTACATG107400.026.8808381
ATCAACG159100.026.863073
TCAACGC160150.026.6578794
TACATGG104450.026.5828112
CAACGCA161700.026.4280555
AACGCAG166250.025.7147856
ACATGGG104250.025.4462763
CATGGGT33200.024.3437254
GGACCGA4150.024.036616
GACCGAT1201.7112658E-423.750467
GAGTACT91550.022.6717312-13
AGTACTT95350.022.5153812-13
ACGCAGA188000.022.461877
CGCAGAG187200.022.4050928
AGAGTAC147750.021.32996610-11
GTACTTT97750.021.0639114-15
GCAGAGT197700.020.9268269
Produced by FastQC (version 0.11.5)