##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937437_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2153847 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.718470717743646 33.0 31.0 34.0 30.0 34.0 2 31.819422642369677 33.0 31.0 34.0 30.0 34.0 3 31.865673374199744 33.0 31.0 34.0 30.0 34.0 4 35.4993571966811 37.0 35.0 37.0 33.0 37.0 5 35.48951202197742 37.0 35.0 37.0 33.0 37.0 6 35.55340885401795 37.0 35.0 37.0 33.0 37.0 7 35.501993874216694 37.0 35.0 37.0 33.0 37.0 8 35.49141698551475 37.0 35.0 37.0 33.0 37.0 9 37.11100649210459 39.0 37.0 39.0 33.0 39.0 10-11 37.023955972731585 39.0 37.0 39.0 33.0 39.0 12-13 36.925688547050925 39.0 37.0 39.0 33.0 39.0 14-15 38.087720019109994 40.0 38.0 41.0 33.0 41.0 16-17 38.04311169734898 40.0 38.0 41.0 33.0 41.0 18-19 38.01828379638851 40.0 38.0 41.0 33.0 41.0 20-21 37.95526632114537 40.0 38.0 41.0 33.0 41.0 22-23 37.82257699827332 40.0 38.0 41.0 33.0 41.0 24-25 37.682396196201495 40.0 37.0 41.0 32.0 41.0 26-27 37.52907379214958 40.0 37.0 41.0 32.0 41.0 28-29 37.35057202298957 39.5 37.0 41.0 32.0 41.0 30-31 37.337529081685005 39.0 37.0 41.0 32.0 41.0 32-33 37.17250645008676 39.0 36.0 41.0 31.5 41.0 34-35 36.971149993476786 39.0 36.0 41.0 31.0 41.0 36-37 36.98037488270987 39.0 36.0 41.0 31.0 41.0 38-39 36.95026921596566 40.0 36.0 41.0 31.0 41.0 40-41 36.78981631471502 39.5 36.0 41.0 30.5 41.0 42-43 36.54644224961197 39.0 36.0 41.0 30.0 41.0 44-45 36.295812330216584 39.0 36.0 41.0 30.0 41.0 46-47 35.94716918146925 39.0 35.0 41.0 28.5 41.0 48-49 35.619441167362396 39.0 35.0 41.0 27.0 41.0 50-51 34.68209139274981 38.0 34.0 40.0 25.5 40.5 52-53 34.80281445246575 38.5 34.5 40.0 26.0 40.5 54-55 35.16924437993971 39.0 35.0 40.0 26.0 41.0 56-57 34.961674622199254 39.0 35.0 40.0 25.5 41.0 58-59 34.732044105268386 38.0 34.0 40.0 25.0 41.0 60-61 34.464793228116946 38.0 34.0 40.0 24.0 41.0 62-63 34.16070616900829 38.0 34.0 40.0 23.0 41.0 64-65 33.82134942732701 37.0 34.0 40.0 22.0 41.0 66-67 33.4427203975027 37.0 33.0 39.5 21.5 41.0 68-69 32.960106265672536 36.0 32.5 39.0 20.0 41.0 70-71 32.54117794810867 36.0 32.0 39.0 18.5 40.0 72-73 32.13981471292993 35.0 32.0 38.5 17.5 40.0 74-75 31.69074892506292 35.0 31.0 37.5 14.0 39.5 76-77 31.257805452290714 35.0 31.0 37.0 9.5 39.0 78-79 30.788480565239777 35.0 31.0 36.5 7.0 39.0 80-81 30.34685657802063 34.5 30.5 36.0 4.5 38.0 82-83 29.91348155184653 34.0 30.0 35.5 2.0 37.0 84-85 29.464365853284843 34.0 29.5 35.0 2.0 37.0 86-87 29.0589176018538 34.0 29.0 35.0 2.0 36.0 88-89 28.689779729015108 34.0 29.0 35.0 2.0 36.0 90-91 28.26794405545055 34.0 29.0 35.0 2.0 35.5 92-93 27.85822994855252 34.0 27.5 35.0 2.0 35.0 94-95 27.367255195006887 33.5 26.5 35.0 2.0 35.0 96-97 26.789529154113545 33.0 25.5 35.0 2.0 35.0 98-99 26.126687271658575 33.0 24.5 35.0 2.0 35.0 100-101 24.302648238245336 31.0 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17000697354055205 1101 2 0.10726403908975612 1101 3 0.22150561946735436 1101 4 0.1837492058873451 1101 5 0.32572900276846894 1101 6 0.20734245722334776 1101 7 0.2916822992709527 1101 8 0.2525657839164168 1101 9 0.2382853563186842 1101 10-11 0.2868381823578545 1101 12-13 0.4026069055670831 1101 14-15 0.4745954266051484 1101 16-17 0.4515881810639044 1101 18-19 0.5254453178408198 1101 20-21 0.4919071676950679 1101 22-23 0.5277917441683613 1101 24-25 0.5150977578890235 1101 26-27 0.5099047671551489 1101 28-29 0.5149396922213967 1101 30-31 0.4651831173739538 1101 32-33 0.4091057812847154 1101 34-35 0.36738030590449 1101 36-37 0.5370524868991922 1101 38-39 0.6709502934933056 1101 40-41 0.7119534623269956 1101 42-43 0.629210888013553 1101 44-45 0.6355012028556786 1101 46-47 0.8734073673201621 1101 48-49 1.1348892932250294 1101 50-51 0.9980330494078018 1101 52-53 0.8998994277240371 1101 54-55 0.9500456652886342 1101 56-57 1.1167907657414773 1101 58-59 0.9848055487243599 1101 60-61 1.0675003670744942 1101 62-63 1.2815549305802634 1101 64-65 1.0199120547769525 1101 66-67 1.0433064899341105 1101 68-69 1.0014905277834032 1101 70-71 1.0124799725972622 1101 72-73 0.9948583597938168 1101 74-75 1.048380338319344 1101 76-77 0.9751937645053523 1101 78-79 1.0787939870398695 1101 80-81 1.083931626354289 1101 82-83 1.1370260364719478 1101 84-85 1.2424375118602171 1101 86-87 1.2623051658455218 1101 88-89 1.2103119766179482 1101 90-91 1.3054549243157858 1101 92-93 1.2856586509203787 1101 94-95 1.2634893936451697 1101 96-97 1.339418522265106 1101 98-99 1.680875659149354 1101 100-101 1.9408367388674925 1102 1 0.12934833057693496 1102 2 0.0530902412649894 1102 3 0.041703819655065644 1102 4 0.03193283090804755 1102 5 0.044085983942210305 1102 6 0.12653585423102243 1102 7 0.1109129623306302 1102 8 0.17736979208734738 1102 9 0.34240532540166413 1102 10-11 0.12327817021188991 1102 12-13 0.37520807599716477 1102 14-15 0.371383645011818 1102 16-17 0.4441059859554244 1102 18-19 0.4371130426793357 1102 20-21 0.45322155515159324 1102 22-23 0.5338433369470224 1102 24-25 0.5159183011452484 1102 26-27 0.46102895972401825 1102 28-29 0.43365070088919566 1102 30-31 0.4736535529726993 1102 32-33 0.3550451618401169 1102 34-35 0.49903229618772116 1102 36-37 0.4730701261638046 1102 38-39 0.6580279449038997 1102 40-41 0.6921603579268307 1102 42-43 0.574242771191372 1102 44-45 0.6159855235093517 1102 46-47 0.714747841013093 1102 48-49 0.8082067999881275 1102 50-51 0.7449351363067152 1102 52-53 0.6837363128341849 1102 54-55 0.8866761240751444 1102 56-57 0.9162131419353656 1102 58-59 0.7240025343148275 1102 60-61 0.8197087257119335 1102 62-63 0.857944816297703 1102 64-65 0.8852704989882909 1102 66-67 0.9054828273713085 1102 68-69 0.9431636354960986 1102 70-71 0.8876166147879516 1102 72-73 0.753807733172053 1102 74-75 0.7975915402185372 1102 76-77 0.7796201433492804 1102 78-79 1.0134907517944676 1102 80-81 0.8451625102498355 1102 82-83 0.883618263049545 1102 84-85 1.0013661818762287 1102 86-87 1.0845695615833861 1102 88-89 1.2365293343168382 1102 90-91 1.028379757308663 1102 92-93 0.7779956464484528 1102 94-95 0.7954264002150389 1102 96-97 0.9587230273167258 1102 98-99 1.0619265618159446 1102 100-101 1.422594316298067 1103 1 0.11988273352824308 1103 2 0.09570152445852997 1103 3 0.10948911878846701 1103 4 0.12294316745487777 1103 5 -0.022740487362561623 1103 6 0.040030790367168834 1103 7 0.16817986797919104 1103 8 0.1623034920142601 1103 9 0.19792424093432714 1103 10-11 0.21579722495729925 1103 12-13 0.20125130155662418 1103 14-15 0.2839375899531831 1103 16-17 0.2803021715260243 1103 18-19 0.25613385498574104 1103 20-21 0.25116555682213715 1103 22-23 0.22686094736013018 1103 24-25 0.19254970058143783 1103 26-27 0.2403984999739066 1103 28-29 0.1505224163383332 1103 30-31 0.19779260911023755 1103 32-33 0.12880442818890714 1103 34-35 0.15829908002511672 1103 36-37 0.27164580569604624 1103 38-39 0.2800239947420593 1103 40-41 0.3780407045665868 1103 42-43 0.4135721137591517 1103 44-45 0.2398531147807148 1103 46-47 0.3692100447509503 1103 48-49 0.3052333026157683 1103 50-51 0.28152024750290394 1103 52-53 0.2489687981298374 1103 54-55 0.27360361163299984 1103 56-57 0.33918498615630455 1103 58-59 0.2989551149712142 1103 60-61 0.37548910019423687 1103 62-63 0.5201581250367795 1103 64-65 0.39740463047473895 1103 66-67 0.4270738551094553 1103 68-69 0.46767943614344176 1103 70-71 0.5239437473532931 1103 72-73 0.5702670308437234 1103 74-75 0.5741307112065961 1103 76-77 0.5499130121760665 1103 78-79 0.5242853955838385 1103 80-81 0.5380965930118151 1103 82-83 0.6523841420040561 1103 84-85 0.5852570912835304 1103 86-87 0.7492765187406647 1103 88-89 0.6864427613867612 1103 90-91 0.8132734335694067 1103 92-93 0.7780278393973283 1103 94-95 0.7084919520195818 1103 96-97 0.8784381632231764 1103 98-99 0.8977386071993045 1103 100-101 1.1996359583124736 1104 1 0.15465850648589097 1104 2 0.21660107603035783 1104 3 0.1864463379982304 1104 4 0.09852866813344008 1104 5 0.04679931009752636 1104 6 0.11927809448543769 1104 7 0.028414370600565064 1104 8 0.1756720246408463 1104 9 0.2329351557675281 1104 10-11 0.24629500527673542 1104 12-13 0.25065925716128135 1104 14-15 0.28520314045900363 1104 16-17 0.23842566679510924 1104 18-19 0.19471431333098366 1104 20-21 0.24372999407545848 1104 22-23 0.3363028179906351 1104 24-25 0.27152428504462023 1104 26-27 0.33844868833792674 1104 28-29 0.23112597594749218 1104 30-31 0.20591122358727176 1104 32-33 0.18030257219214718 1104 34-35 0.3257429876304343 1104 36-37 0.026165289558768734 1104 38-39 0.2446218390732966 1104 40-41 0.22922958776361924 1104 42-43 0.20650213529511063 1104 44-45 0.26558024481451525 1104 46-47 0.17614154693780648 1104 48-49 0.3331929846818369 1104 50-51 0.16557146040131698 1104 52-53 0.26752427373062915 1104 54-55 0.30479904286610093 1104 56-57 0.47905941590269663 1104 58-59 0.30773924488148197 1104 60-61 0.38620446881498083 1104 62-63 0.4911509299718233 1104 64-65 0.4808043224233387 1104 66-67 0.4896155470079435 1104 68-69 0.275674259912293 1104 70-71 0.3790330312935879 1104 72-73 0.3798872879448396 1104 74-75 0.4350215723769999 1104 76-77 0.5233911337017396 1104 78-79 0.41102458592522595 1104 80-81 0.6449017129194843 1104 82-83 0.44954205247218937 1104 84-85 0.3872176147463193 1104 86-87 0.4428197059756229 1104 88-89 0.47203440408859265 1104 90-91 0.5794899731483127 1104 92-93 0.5662964885582191 1104 94-95 0.5379132801306135 1104 96-97 0.5754506001724096 1104 98-99 0.5804958486965752 1104 100-101 0.73014522793002 1105 1 0.16696955780434308 1105 2 0.2539875493407635 1105 3 0.19856278820396867 1105 4 0.26605772894122026 1105 5 0.19129813536672202 1105 6 0.06672135832479853 1105 7 0.06274534397358167 1105 8 0.12848462939361127 1105 9 0.25827060834073023 1105 10-11 0.25975510304973426 1105 12-13 0.29903356921447255 1105 14-15 0.33290778471392457 1105 16-17 0.2780722689246957 1105 18-19 0.3698247560241512 1105 20-21 0.3434131017239892 1105 22-23 0.3940913806945687 1105 24-25 0.4301143493642243 1105 26-27 0.4408523682862153 1105 28-29 0.3327292228989265 1105 30-31 0.3651846415252251 1105 32-33 0.2989248863030909 1105 34-35 0.48167640930292066 1105 36-37 0.44916230417522485 1105 38-39 0.46419844854448655 1105 40-41 0.5606941504885441 1105 42-43 0.39007482049800046 1105 44-45 0.4218848709216587 1105 46-47 0.186554768714835 1105 48-49 0.17597206479975824 1105 50-51 0.07895278034429509 1105 52-53 0.1216354940783333 1105 54-55 0.12973293848040868 1105 56-57 0.23769188751681014 1105 58-59 0.21088544618527294 1105 60-61 0.3938933687525079 1105 62-63 0.4476431455523624 1105 64-65 0.4722021283426585 1105 66-67 0.597915378203659 1105 68-69 0.5181919596146543 1105 70-71 0.5125188021652107 1105 72-73 0.35729499864834935 1105 74-75 0.4045864645364894 1105 76-77 0.4888719334335754 1105 78-79 0.5020406324555111 1105 80-81 0.48035466107010194 1105 82-83 0.5578659744607641 1105 84-85 0.5834913971395252 1105 86-87 0.5504778971826525 1105 88-89 0.5876936444168415 1105 90-91 0.7835249278576235 1105 92-93 0.8483723949907045 1105 94-95 0.9690687307990444 1105 96-97 0.9419717659493116 1105 98-99 1.2387865122676196 1105 100-101 1.3998243685655076 1106 1 -0.04299307862888213 1106 2 0.03319322560525961 1106 3 -0.020716651663708063 1106 4 -0.009961605901644077 1106 5 0.07715591828333146 1106 6 0.0832900660699778 1106 7 0.09842674730204237 1106 8 0.03222125371846829 1106 9 0.020354885130885236 1106 10-11 -0.14299657911371355 1106 12-13 -0.11506100664480812 1106 14-15 -0.12709767529153027 1106 16-17 -0.21466846646310245 1106 18-19 -0.19130054039526811 1106 20-21 0.02208575833783044 1106 22-23 -0.08764637310201095 1106 24-25 -0.1478573389015807 1106 26-27 0.13753300614332176 1106 28-29 0.188015659067716 1106 30-31 0.25310397797397144 1106 32-33 0.2802631764649064 1106 34-35 0.1976115958263378 1106 36-37 0.018188549672061072 1106 38-39 -0.02656236574573967 1106 40-41 0.23527381036232242 1106 42-43 0.1376519430751202 1106 44-45 0.1621679347341356 1106 46-47 0.33828939103769784 1106 48-49 0.4010065913048706 1106 50-51 0.4067257404702005 1106 52-53 0.4430290801778014 1106 54-55 0.5508137786788936 1106 56-57 0.32757789652045943 1106 58-59 0.34588140471064577 1106 60-61 0.5523131521722888 1106 62-63 0.5453411108973896 1106 64-65 0.4587448207677056 1106 66-67 0.407072128536889 1106 68-69 0.4582891423588933 1106 70-71 0.3521826638878949 1106 72-73 0.6084365144957502 1106 74-75 0.5585592876269594 1106 76-77 0.48327676607369696 1106 78-79 0.5189218184543449 1106 80-81 0.6136135884466754 1106 82-83 0.38950041638932476 1106 84-85 0.36931866826091664 1106 86-87 0.3012145964487658 1106 88-89 0.4043592404942018 1106 90-91 0.6027626147423675 1106 92-93 0.4526444477921707 1106 94-95 0.39851020435816054 1106 96-97 0.4429118771835405 1106 98-99 0.834113791418428 1106 100-101 0.9202960434489462 1107 1 0.1188397639857186 1107 2 0.014973051033294382 1107 3 0.11469237299613155 1107 4 0.08087189861264221 1107 5 0.2170156477196059 1107 6 0.07704929761422363 1107 7 0.06733376506899447 1107 8 0.06947675025735123 1107 9 0.146265812344744 1107 10-11 0.3136432855066147 1107 12-13 0.3891704234823905 1107 14-15 0.41426697926530665 1107 16-17 0.3943134796524035 1107 18-19 0.40173467723278833 1107 20-21 0.41569924152785376 1107 22-23 0.4267466844506558 1107 24-25 0.40360210099434113 1107 26-27 0.5440388605319129 1107 28-29 0.4567827975416989 1107 30-31 0.3200283942469184 1107 32-33 0.2800341200469276 1107 34-35 0.4332054959153524 1107 36-37 0.39108343152459923 1107 38-39 0.363947036164312 1107 40-41 0.34815053073089786 1107 42-43 0.3030029618463388 1107 44-45 0.327955057361649 1107 46-47 0.2594687136828142 1107 48-49 0.32008201852361395 1107 50-51 0.20290547068901787 1107 52-53 0.18389779905955095 1107 54-55 0.23736922663499627 1107 56-57 0.28094657464483674 1107 58-59 0.1923924108307702 1107 60-61 0.3149895093111894 1107 62-63 0.42745612493422414 1107 64-65 0.4278246497823801 1107 66-67 0.5228124617039285 1107 68-69 0.44279074493867654 1107 70-71 0.44047942970909304 1107 72-73 0.3288703033334457 1107 74-75 0.2330301754528996 1107 76-77 0.13100266787126102 1107 78-79 0.36991776554150135 1107 80-81 0.39120632776580067 1107 82-83 0.25551137849149086 1107 84-85 0.3114521698406705 1107 86-87 0.43792948723531566 1107 88-89 0.49824628714997843 1107 90-91 0.6037041099835392 1107 92-93 0.6101700515718456 1107 94-95 0.5815539973368118 1107 96-97 0.8502084245452366 1107 98-99 0.9692171358051596 1107 100-101 1.2563226237752012 1108 1 -0.022645450769903874 1108 2 -0.09636204158929829 1108 3 -0.03522228399847904 1108 4 -0.1456352490040942 1108 5 0.015738124096245087 1108 6 -0.026867966498294038 1108 7 -0.09520601050735422 1108 8 -0.045039044136132134 1108 9 -0.02520573946399196 1108 10-11 -0.016937069335668298 1108 12-13 0.06201319921602533 1108 14-15 0.10425935239236139 1108 16-17 0.0975173264284308 1108 18-19 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-1.2566871739183831 2316 76-77 -0.9354719407336667 2316 78-79 -1.3182498504212319 2316 80-81 -1.1852358895229145 2316 82-83 -1.168689757024314 2316 84-85 -1.4408043038732288 2316 86-87 -1.3819203541442882 2316 88-89 -1.149223672027258 2316 90-91 -1.5673841381557807 2316 92-93 -1.673538299200633 2316 94-95 -1.591635667770948 2316 96-97 -1.6503682045741925 2316 98-99 -1.6723682492814866 2316 100-101 -1.7203987714777114 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7734.0 3 1264.0 4 1046.0 5 1275.0 6 1495.0 7 1859.0 8 2301.0 9 2930.0 10 3767.0 11 5105.0 12 7103.0 13 9673.0 14 11280.0 15 14279.0 16 18378.0 17 24249.0 18 20130.0 19 13376.0 20 12617.0 21 13253.0 22 14244.0 23 15586.0 24 17425.0 25 19933.0 26 23065.0 27 27032.0 28 32472.0 29 39738.0 30 48541.0 31 60441.0 32 77566.0 33 101965.0 34 138600.0 35 196130.0 36 294347.0 37 431683.0 38 386952.0 39 55004.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.17724350013978 19.240052185257664 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27.209713961062075 31.061372761692844 20.905741112728833 20-21 22.124271017893435 26.07257654530246 30.961005782996292 20.84214665380781 22-23 21.403545018637605 26.19469240682536 30.997426495993164 21.404336078543874 24-25 21.13928456104663 26.253939790417576 31.236756860376712 21.370018788159086 26-27 20.988411512078265 26.52471470857999 31.01643459133164 21.470439188010104 28-29 20.858957943258734 27.029502844369514 30.86121724449479 21.25032196787697 30-31 21.181335903566012 26.483767184203682 30.871623486354327 21.463273425875983 32-33 20.821099083577323 26.5104266291739 30.880060837322993 21.78841344992579 34-35 21.420634019495978 26.600451568110284 30.726408544428086 21.252505867965652 36-37 21.27363647552588 26.47029330134379 30.731414162306763 21.524656060823567 38-39 21.02266501746711 26.83290641645484 30.60536898668908 21.539059579388976 40-41 21.52037285009599 26.653296310116588 30.43895589317735 21.387374946610077 42-43 21.114278083773716 26.679154650936642 30.2539564669985 21.952610798291136 44-45 21.36332118741478 26.863165291525316 30.10079716316017 21.67271635789973 46-47 21.572594435559633 27.10532401537194 29.502664124840454 21.81941742422797 48-49 21.47194070280927 27.08270656048233 29.512316624587086 21.933036112121314 50-51 21.54771650406455 27.442208106199978 29.303472190872533 21.706603198862943 52-53 21.64448839320602 27.532073532618572 28.946318097857464 21.87711997631794 54-55 21.582515668356763 27.345296793536995 29.093017865603528 21.97916967250272 56-57 21.651892989353925 27.59225581095648 28.774080210064895 21.981770989624696 58-59 21.790852877681278 27.448018833651428 28.869590174572245 21.89153811409505 60-61 21.531630797673103 27.433238034694103 29.327999214722468 21.70713195291033 62-63 21.295283359226875 27.346742147558057 29.441036814729333 21.916937678485738 64-65 21.336646836510063 27.5265539256825 29.084271032402487 22.052528205404943 66-67 21.22573397407638 27.48879796936223 29.05025437641661 22.235213680144774 68-69 21.48647815675406 27.395174167946955 29.08622740980495 22.032120265494033 70-71 21.522743074819147 27.204132765654947 28.943665526611028 22.32945863291488 72-73 21.335372036114077 27.13218080861616 29.1827336268234 22.349713528446372 74-75 21.279242779391197 27.52666639991737 28.842585656766236 22.351505163925196 76-77 21.315828046150305 27.244266692724512 28.987792365517095 22.45211289560809 78-79 21.294643210267736 27.30975409679696 28.896895046899306 22.498707646036 80-81 21.444714792811318 27.383960846526385 28.73437420039172 22.436950160270573 82-83 21.359570470448208 27.311154870176097 29.074556264779154 22.254718394596544 84-85 21.31664446115027 27.32294977335487 28.899208044599593 22.461197720895267 86-87 21.237376233881182 27.514651884710982 28.804506232357546 22.44346564905029 88-89 21.404087759149025 27.23973895961653 28.524166149431007 22.832007131803433 90-91 21.3361986743998 26.950528667523354 28.826965719193474 22.886306938883372 92-93 21.322556577618535 26.77716082800362 29.05957513830188 22.840707456075965 94-95 21.031316941894204 26.785155951931184 29.142720351080282 23.04080675509433 96-97 20.801300840242302 26.663107500767662 29.396267760935714 23.13932389805432 98-99 20.889937228360626 26.943624637600916 29.070676371277195 23.095761762761263 100-101 20.676273668124658 26.76501612473673 29.324459010140036 23.234251196998578 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3146.0 1 1798.0 2 449.0 3 428.5 4 369.0 5 399.0 6 608.0 7 1082.0 8 1739.0 9 2125.5 10 2094.0 11 2117.5 12 2304.0 13 2473.5 14 2738.5 15 2999.5 16 3256.5 17 3518.0 18 3764.5 19 4180.0 20 4734.5 21 5339.5 22 6172.0 23 7390.5 24 8890.5 25 10903.5 26 13708.0 27 17146.0 28 20712.0 29 24838.5 30 29382.0 31 34015.5 32 39361.5 33 43725.0 34 47760.0 35 52818.0 36 58156.0 37 62427.5 38 65268.5 39 67861.5 40 70653.5 41 73819.5 42 75623.0 43 76842.5 44 79087.5 45 81395.5 46 83694.5 47 87156.5 48 90776.0 49 89076.5 50 82052.5 51 75334.5 52 70420.0 53 66598.0 54 63247.5 55 57686.5 56 50170.5 57 43437.0 58 38729.5 59 33633.5 60 26643.5 61 20751.5 62 16071.0 63 11523.5 64 7992.5 65 5791.5 66 4160.5 67 3076.0 68 2298.5 69 1642.0 70 1153.5 71 772.5 72 526.0 73 401.5 74 298.5 75 202.5 76 143.0 77 93.5 78 66.5 79 42.5 80 30.0 81 23.0 82 14.5 83 11.0 84 9.5 85 7.5 86 7.5 87 6.5 88 3.5 89 4.0 90 3.5 91 1.5 92 0.5 93 1.5 94 3.0 95 2.5 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35503914623462113 2 0.314042733768926 3 0.31659630419430906 4 0.316828446960253 5 0.30169273862070983 6 0.22801062471011174 7 0.23543919322031698 8 0.23191062317796948 9 0.22875348156113223 10-11 0.23945526307114665 12-13 0.2390374060924476 14-15 0.2197695565191028 16-17 0.23383740813530396 18-19 0.21979277079569717 20-21 0.22631598251872115 22-23 0.22431955473160348 24-25 0.21454634428536476 26-27 0.22768562483779023 28-29 0.22993740966744622 30-31 0.23882847760309808 32-33 0.2398499057732513 34-35 0.22708205364633605 36-37 0.22339098366782784 38-39 0.20992670324308085 40-41 0.21194634530679293 42-43 0.21268920215781342 44-45 0.2310749092205714 46-47 0.21988562790207475 48-49 0.2294731241355584 50-51 0.23892133470947563 52-53 0.25004097319818913 54-55 0.24370347568792028 56-57 0.2050284908816643 58-59 0.18933563990385574 60-61 0.1991088503500945 62-63 0.2015231351159112 64-65 0.19773920803102543 66-67 0.21224813090252004 68-69 0.19822670783950763 70-71 0.20384456277535035 72-73 0.2213713416041158 74-75 0.20953206054097623 76-77 0.22202134134875876 78-79 0.21672848628523753 80-81 0.20228920624352612 82-83 0.2184463427532225 84-85 0.19643920854173952 86-87 0.20333384869027374 88-89 0.21361777322158906 90-91 0.2080463468389352 92-93 0.21605527226400018 94-95 0.2133392019024564 96-97 0.20739634709429222 98-99 0.21487134415768624 100-101 0.19497670911629283 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2153847.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.887434267133244 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2725042385398 44.32604645469521 2 14.059199151779788 16.558203317979356 3 4.87919105525258 8.619691276289124 4 2.179690411044129 5.1342550321224705 5 1.1070379457532629 3.2595312130883736 6 0.6780472826259565 2.3957078871386597 7 0.4160391504796909 1.7149634688498734 8 0.2883637721117583 1.3584802140203005 9 0.18352278395054475 0.9726467288768099 >10 0.865318394752913 8.68064046772669 >50 0.04511616732713156 1.7942968362933611 >100 0.023130084164375772 2.62576128654901 >500 0.0018930414786585432 0.7167929667617112 >1k 7.098905544968184E-4 0.7701158235424677 >5k 1.577534565548485E-4 0.5850650731809965 >10k+ 7.887672827742426E-5 0.4878019528855848 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10502 0.48759266558859565 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7318 0.3397641522355116 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5278 0.24504990373039495 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3421 0.15883208045882552 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2928 0.13594280373675569 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.3214276594391337E-5 0.0 16-17 0.0 0.0 0.0 9.285710637756535E-5 0.0 18-19 0.0 0.0 0.0 9.285710637756535E-5 0.0 20-21 0.0 0.0 0.0 1.1607138297195669E-4 0.0 22-23 0.0 0.0 0.0 1.3928565956634802E-4 0.0 24-25 0.0 0.0 0.0 3.714284255102614E-4 0.0 26-27 0.0 0.0 0.0 6.732140212373488E-4 0.0 28-29 0.0 0.0 0.0 0.0018571421275513072 0.0 30-31 0.0 0.0 0.0 0.0048517838082277895 0.0 32-33 0.0 0.0 0.0 0.007428568510205228 0.0 34-35 0.0 0.0 0.0 0.00988928182921071 0.0 36-37 0.0 0.0 0.0 0.013626780360907715 0.0 38-39 0.0 0.0 0.0 0.01961606372226068 0.0 40-41 0.0 0.0 0.0 0.02720713216862665 0.0 42-43 0.0 0.0 0.0 0.03426427225332161 0.0 44-45 0.0 0.0 0.0 0.03781605657226349 0.0 46-47 0.0 0.0 0.0 0.04336426867832302 0.0 48-49 0.0 0.0 0.0 0.05295176491180664 0.0 50-51 0.0 0.0 0.0 0.06260890397507343 0.0 52-53 0.0 0.0 0.0 0.08614818044178625 0.0 54-55 0.0 0.0 0.0 0.10044817482393131 0.0 56-57 0.0 0.0 0.0 0.14703922794887475 0.0 58-59 0.0 0.0 0.0 0.2345338364331357 0.0 60-61 0.0 0.0 0.0 0.3119302345988364 0.0 62-63 0.0 0.0 0.0 0.35283378995815395 0.0 64-65 0.0 0.0 0.0 0.42693376084745105 0.0 66-67 0.0 0.0 0.0 0.5776408444982397 0.0 68-69 0.0 0.0 0.0 0.7882407617625579 0.0 70-71 0.0 0.0 0.0 0.9818014000065929 0.0 72-73 0.0 0.0 0.0 1.2092548820784392 0.0 74-75 0.0 0.0 0.0 1.4930958419980622 0.0 76-77 0.0 0.0 0.0 1.6956404052841265 0.0 78-79 0.0 0.0 0.0 1.9721456537999216 0.0 80-81 0.0 0.0 0.0 2.2644133961233086 0.0 82-83 0.0 0.0 0.0 2.484043666982845 0.0 84-85 0.0 0.0 0.0 2.814266751538062 0.0 86-87 0.0 0.0 0.0 3.273421928298528 0.0 88-89 0.0 0.0 0.0 3.924953815196716 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13185 0.0 32.57362 1 GGTATCA 8760 0.0 30.784874 1 TATCAAC 15925 0.0 26.899355 2 GTACATG 10740 0.0 26.880838 1 ATCAACG 15910 0.0 26.86307 3 TCAACGC 16015 0.0 26.657879 4 TACATGG 10445 0.0 26.582811 2 CAACGCA 16170 0.0 26.428055 5 AACGCAG 16625 0.0 25.714785 6 ACATGGG 10425 0.0 25.446276 3 CATGGGT 3320 0.0 24.343725 4 GGACCGA 415 0.0 24.03661 6 GACCGAT 120 1.7112658E-4 23.75046 7 GAGTACT 9155 0.0 22.67173 12-13 AGTACTT 9535 0.0 22.51538 12-13 ACGCAGA 18800 0.0 22.46187 7 CGCAGAG 18720 0.0 22.405092 8 AGAGTAC 14775 0.0 21.329966 10-11 GTACTTT 9775 0.0 21.06391 14-15 GCAGAGT 19770 0.0 20.926826 9 >>END_MODULE