FastQCFastQC Report
Thu 26 May 2016
SRR937437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937437_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2153847
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121320.5632712072863114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92090.4275605463154997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62150.2885534580682843No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40830.18956778266979968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA144500.031.6117731
GGTATCA97400.031.1354431
TATCAAC179000.025.4534742
ATCAACG179000.025.2676813
TCAACGC179000.025.2676814
CAACGCA181300.024.9471325
TACATGG101850.024.7227252
GTACATG104800.024.6281571
AACGCAG186250.024.2524056
ACATGGG102500.023.2217713
GAGTACT107850.022.61627812-13
CATGGGT32400.022.1418274
GGACCGA4300.022.0918526
AGTACTT111100.021.52713412-13
AGAGTAC161100.021.52425610-11
ACGCAGA209850.021.322737
CGCAGAG209000.021.273088
GTACTTT114850.021.05025514-15
GCAGAGT217800.019.9991869
GTATAGC10750.019.897451
Produced by FastQC (version 0.11.5)