##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937437_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2153847 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18099707175115 33.0 31.0 34.0 31.0 34.0 2 32.40376591280625 34.0 31.0 34.0 31.0 34.0 3 32.48469645244068 34.0 31.0 34.0 31.0 34.0 4 36.091048714230865 37.0 35.0 37.0 35.0 37.0 5 35.99922000030643 37.0 35.0 37.0 35.0 37.0 6 36.076362898571716 37.0 35.0 37.0 35.0 37.0 7 36.03226691589514 37.0 35.0 37.0 35.0 37.0 8 36.00868678230162 37.0 35.0 37.0 35.0 37.0 9 37.71161461329426 39.0 37.0 39.0 35.0 39.0 10-11 37.63825517782832 39.0 37.0 39.0 35.0 39.0 12-13 37.57176856109092 39.0 37.0 39.0 35.0 39.0 14-15 38.791413921230244 40.0 38.0 41.0 35.0 41.0 16-17 38.781993335645474 40.0 38.0 41.0 35.0 41.0 18-19 38.80748214706058 40.0 38.0 41.0 35.0 41.0 20-21 38.70775616838151 40.0 38.0 41.0 34.0 41.0 22-23 38.58318278874962 40.0 38.0 41.0 34.0 41.0 24-25 38.45409051803587 40.0 38.0 41.0 34.0 41.0 26-27 38.40123253880151 40.0 38.0 41.0 34.0 41.0 28-29 38.30725812929145 40.0 38.0 41.0 34.0 41.0 30-31 38.35382503956873 40.0 38.0 41.0 34.0 41.0 32-33 38.2546624713826 40.0 38.0 41.0 34.0 41.0 34-35 38.14093317677626 40.0 38.0 41.0 34.0 41.0 36-37 37.986349076791434 40.0 37.0 41.0 33.5 41.0 38-39 37.84926714850219 40.0 37.0 41.0 33.0 41.0 40-41 37.67413493158985 40.0 37.0 41.0 33.0 41.0 42-43 37.447230699302224 40.0 37.0 41.0 33.0 41.0 44-45 37.12455062964082 40.0 37.0 41.0 32.0 41.0 46-47 36.72922565994706 40.0 36.0 41.0 31.0 41.0 48-49 36.384200224064195 40.0 36.0 41.0 30.0 41.0 50-51 36.14495110376921 39.0 36.0 41.0 30.0 41.0 52-53 35.919923513601475 39.0 35.5 41.0 29.0 41.0 54-55 35.7009063317868 39.0 35.0 40.5 28.0 41.0 56-57 35.44503741444959 39.0 35.0 40.0 28.0 41.0 58-59 35.21397225522519 38.5 35.0 40.0 27.5 41.0 60-61 34.89598007657926 38.0 34.5 40.0 26.5 41.0 62-63 34.535970985868545 38.0 34.0 40.0 26.0 41.0 64-65 34.20092954606339 37.0 34.0 40.0 26.0 41.0 66-67 33.78001872927835 37.0 33.5 39.5 25.0 41.0 68-69 33.3910208106704 36.0 33.0 39.0 24.0 40.5 70-71 32.98415277408284 36.0 33.0 39.0 23.0 40.0 72-73 32.5438968041834 35.0 32.0 38.0 22.0 40.0 74-75 32.1061326547336 35.0 32.0 37.0 21.0 39.0 76-77 30.76519641367284 34.0 30.5 36.0 20.0 38.5 78-79 31.379492600913622 35.0 31.0 36.0 20.0 39.0 80-81 31.23836813849823 35.0 31.5 36.0 19.5 37.5 82-83 30.909122839273174 34.5 31.0 35.5 18.0 37.0 84-85 30.550466908745143 34.0 31.0 35.0 16.5 37.0 86-87 30.19682410124767 34.0 31.0 35.0 10.0 36.0 88-89 29.80585366555749 34.0 30.5 35.0 7.0 36.0 90-91 29.39296547062071 34.0 30.0 35.0 2.0 35.5 92-93 29.062089600607656 34.0 29.5 35.0 2.0 35.0 94-95 28.687975283295422 34.0 29.0 35.0 2.0 35.0 96-97 28.250397312343914 34.0 29.0 35.0 2.0 35.0 98-99 27.817775589445304 34.0 29.0 35.0 2.0 35.0 100-101 26.006402497484732 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16421558431758854 1101 2 0.18572536587546296 1101 3 0.13400285031129044 1101 4 0.16864884481951492 1101 5 -0.007328721248164527 1101 6 0.07189160533181393 1101 7 0.11483798672968959 1101 8 0.039744121976077906 1101 9 0.19311065722182974 1101 10-11 0.08825410769694741 1101 12-13 0.15836651373732025 1101 14-15 0.13410082409084367 1101 16-17 0.1970494048654814 1101 18-19 0.06872880651869195 1101 20-21 0.26328647231975566 1101 22-23 0.13005276786883968 1101 24-25 0.24722050720425415 1101 26-27 0.12121929966485823 1101 28-29 0.13036477448169848 1101 30-31 0.1798426611088928 1101 32-33 0.17303335350883486 1101 34-35 0.1668091307256674 1101 36-37 0.07312505970661931 1101 38-39 0.08410726665660917 1101 40-41 -0.037711604085536976 1101 42-43 0.055599519101519945 1101 44-45 0.16713274930834388 1101 46-47 0.2898706340376975 1101 48-49 0.24441536476825831 1101 50-51 0.08141289521908845 1101 52-53 -0.004281750821654384 1101 54-55 0.1753340236616694 1101 56-57 0.11657873821859965 1101 58-59 -0.04011233527810276 1101 60-61 0.04832655061261448 1101 62-63 0.07596057504998299 1101 64-65 0.25113514831201655 1101 66-67 0.16391199252845468 1101 68-69 0.15637833084864639 1101 70-71 0.12941799664590548 1101 72-73 0.0616581377061749 1101 74-75 0.1367327102893725 1101 76-77 0.17355866950819632 1101 78-79 0.2058502266280655 1101 80-81 0.25921073386754045 1101 82-83 0.14535734311178672 1101 84-85 0.2182953756550141 1101 86-87 0.2953141354982911 1101 88-89 0.1960313582206119 1101 90-91 0.3386825821149948 1101 92-93 0.15174585744503233 1101 94-95 0.15175022376486424 1101 96-97 0.20791108939079805 1101 98-99 0.31380472031734996 1101 100-101 0.7751065422882633 1102 1 0.15957665095481133 1102 2 0.16128021598311904 1102 3 0.17305491236616888 1102 4 0.13926939359664203 1102 5 0.034805381330087926 1102 6 0.03987730620963248 1102 7 0.11737759916275081 1102 8 0.1396060995612416 1102 9 0.19051583582283627 1102 10-11 0.1870001740581273 1102 12-13 0.14798101713267187 1102 14-15 0.14268236773353493 1102 16-17 0.15699460996679448 1102 18-19 0.17595497004764127 1102 20-21 0.09807571209230304 1102 22-23 0.1436072591182196 1102 24-25 0.16374248037269723 1102 26-27 0.15013223512557516 1102 28-29 0.15156915807359894 1102 30-31 0.2747102976130691 1102 32-33 0.21995200309752505 1102 34-35 0.1576368511474655 1102 36-37 0.23734550910759822 1102 38-39 0.1358573908767866 1102 40-41 0.17920235274105067 1102 42-43 0.059016954092960816 1102 44-45 0.06086308111422767 1102 46-47 0.0776632415571612 1102 48-49 0.034715149453290906 1102 50-51 0.07125168503855406 1102 52-53 0.1346681746462437 1102 54-55 0.12145490369258738 1102 56-57 0.06813701128213978 1102 58-59 0.04787971463080254 1102 60-61 0.06613213223907621 1102 62-63 0.023413371384101822 1102 64-65 -0.12239767341823438 1102 66-67 0.020178900274267164 1102 68-69 0.08705381254687694 1102 70-71 0.04935464435749992 1102 72-73 0.011769935862197656 1102 74-75 -0.00808661836959601 1102 76-77 0.17991460171158735 1102 78-79 0.051974557219352846 1102 80-81 0.1065841673131338 1102 82-83 0.19322903747495346 1102 84-85 0.17176732950032303 1102 86-87 0.18437861957050572 1102 88-89 0.16483484190636233 1102 90-91 0.12277273835636393 1102 92-93 0.30278585634085076 1102 94-95 0.337620869157675 1102 96-97 0.16303655176588805 1102 98-99 0.10653576983979107 1102 100-101 0.6618260255323385 1103 1 0.1564173915666487 1103 2 0.14921569087261588 1103 3 0.07310885730752403 1103 4 0.10187075788160627 1103 5 0.05741788263521386 1103 6 0.035727896123667335 1103 7 0.09186512221985765 1103 8 0.0868914508994223 1103 9 0.12854614939956122 1103 10-11 0.0436928271051471 1103 12-13 0.09865054346128233 1103 14-15 0.1298691390417801 1103 16-17 0.12552469732279548 1103 18-19 0.10571387096297258 1103 20-21 0.1518031335240977 1103 22-23 0.13664183608546665 1103 24-25 0.0282580199961302 1103 26-27 0.09228759655668028 1103 28-29 -0.04561961441796569 1103 30-31 -0.005453625402282114 1103 32-33 0.015687307451408117 1103 34-35 0.009878611492716516 1103 36-37 0.10395054512890312 1103 38-39 0.06432944391684003 1103 40-41 0.12338848019032156 1103 42-43 0.10265362621213114 1103 44-45 0.08434174675999628 1103 46-47 -0.018871231000524347 1103 48-49 0.02901177702437252 1103 50-51 -0.004031960943422064 1103 52-53 0.11311369025546725 1103 54-55 0.08501596588588001 1103 56-57 0.00901640083421995 1103 58-59 0.1360635972744504 1103 60-61 0.139261460885578 1103 62-63 0.15128680959104912 1103 64-65 0.010853292370228473 1103 66-67 0.2007321839550542 1103 68-69 0.23250460083908564 1103 70-71 -0.0316850138751974 1103 72-73 0.17221828793124416 1103 74-75 0.28226767808395437 1103 76-77 0.2310022013357127 1103 78-79 0.11411477926369429 1103 80-81 0.10946239095842003 1103 82-83 0.05838147543827077 1103 84-85 0.270757602009283 1103 86-87 0.20083704716246942 1103 88-89 0.11290935553180859 1103 90-91 0.15195477643105093 1103 92-93 0.15484751018834686 1103 94-95 0.2870888154215514 1103 96-97 0.2545893830052286 1103 98-99 0.22317865432800943 1103 100-101 0.3931841329349801 1104 1 0.15007011586928343 1104 2 0.12746498099812698 1104 3 0.02919140402706688 1104 4 0.12375899379058808 1104 5 0.08384223165417382 1104 6 0.0740225041874325 1104 7 0.07845408782885244 1104 8 0.07046503726223108 1104 9 0.028478336625013867 1104 10-11 0.0013392141528214552 1104 12-13 0.06971409828467046 1104 14-15 0.07928927503015615 1104 16-17 0.08640873402288918 1104 18-19 0.1170817107762332 1104 20-21 -0.012531234421963688 1104 22-23 0.058413969613575034 1104 24-25 0.16030184518948687 1104 26-27 -0.030571614620939158 1104 28-29 0.02635738279782629 1104 30-31 -0.005415270151459595 1104 32-33 -0.06446834230295906 1104 34-35 0.08186934403480706 1104 36-37 -0.07638824230958363 1104 38-39 -0.11829994674435085 1104 40-41 0.08726621332588991 1104 42-43 -0.028999649231266744 1104 44-45 -0.03971243780650724 1104 46-47 -0.13191210266679576 1104 48-49 -0.27599073248021 1104 50-51 -0.26110768405651186 1104 52-53 -0.18536687166622556 1104 54-55 -0.15465442083698377 1104 56-57 -0.11303700453326826 1104 58-59 -0.05179005498336409 1104 60-61 0.05997977525868947 1104 62-63 -0.09322765526250265 1104 64-65 -0.32031985135225227 1104 66-67 -0.2409317255680179 1104 68-69 -0.13074769982807055 1104 70-71 -0.12444502835344196 1104 72-73 -0.2748223539490624 1104 74-75 -0.2713079556199425 1104 76-77 -0.1570576535881436 1104 78-79 -0.3173520758389792 1104 80-81 -0.32525628105149096 1104 82-83 -0.25796442672647046 1104 84-85 -0.14419844062724252 1104 86-87 -0.1575493197918867 1104 88-89 -0.15121034394961796 1104 90-91 -0.19313812707007472 1104 92-93 -0.3796031933799888 1104 94-95 -0.26210861823206955 1104 96-97 -0.05562097481297812 1104 98-99 0.06406448785067198 1104 100-101 0.2769995992579446 1105 1 0.04914506691903853 1105 2 0.09621631677165965 1105 3 0.10256033549073607 1105 4 0.14078227918230368 1105 5 0.0691183515104612 1105 6 0.09626496142580265 1105 7 0.127717790709319 1105 8 0.11466123717868726 1105 9 0.1310087510166511 1105 10-11 0.11403875528414886 1105 12-13 0.031518661774505574 1105 14-15 0.0840948726430426 1105 16-17 0.16331957446723067 1105 18-19 0.15620928286676872 1105 20-21 0.14688232256391132 1105 22-23 0.12730276351411618 1105 24-25 0.09264290032496802 1105 26-27 0.11120054534642776 1105 28-29 0.15426636946970973 1105 30-31 0.16204601772545857 1105 32-33 0.16363950120925352 1105 34-35 0.14109669887699994 1105 36-37 0.09569810284154556 1105 38-39 0.19382402128857734 1105 40-41 0.3343935971895249 1105 42-43 0.19161876985779003 1105 44-45 0.39197844713736885 1105 46-47 0.27075668848909373 1105 48-49 0.19722836737528127 1105 50-51 0.17668613925167165 1105 52-53 0.30780709122982586 1105 54-55 0.28916163199253475 1105 56-57 0.2328500690784452 1105 58-59 0.14155814902459696 1105 60-61 0.2368593445415641 1105 62-63 0.34348686303037823 1105 64-65 0.193620937415929 1105 66-67 0.37418317532790013 1105 68-69 0.34718138437684587 1105 70-71 0.1642920291727492 1105 72-73 0.32779345621023737 1105 74-75 0.2060935683429861 1105 76-77 0.23059966772566298 1105 78-79 0.38134100333554954 1105 80-81 0.21978094062954767 1105 82-83 0.23455430409932276 1105 84-85 0.21869963863352737 1105 86-87 0.2330961034824064 1105 88-89 0.26505690299581985 1105 90-91 0.23620554599405708 1105 92-93 0.19667275624719238 1105 94-95 0.20770871169068172 1105 96-97 0.23350884700139218 1105 98-99 0.39532395075159954 1105 100-101 0.7098746825914652 1106 1 -0.1396306071507496 1106 2 0.011487202630782178 1106 3 0.02713865712787822 1106 4 -1.8489119663200881E-4 1106 5 -0.06511615037110374 1106 6 -0.16977514284959483 1106 7 -0.08297953763506882 1106 8 -0.022053017182621204 1106 9 -0.08982170510792287 1106 10-11 -0.19252850716546277 1106 12-13 -0.1466180310680727 1106 14-15 0.02356642976166512 1106 16-17 0.04792409877763504 1106 18-19 0.025610769915310527 1106 20-21 -0.007974405822231745 1106 22-23 -0.06829256443415233 1106 24-25 4.491022488153362E-4 1106 26-27 0.011669195815073863 1106 28-29 0.05369119536511846 1106 30-31 0.10207495040144465 1106 32-33 0.06486492449349868 1106 34-35 -0.03133241472858117 1106 36-37 0.08949872164216544 1106 38-39 0.18841821441218798 1106 40-41 0.08576060590947066 1106 42-43 -0.1437152272409179 1106 44-45 -0.11371976444319642 1106 46-47 0.07378096945455326 1106 48-49 0.1850811934634038 1106 50-51 0.12494054338843341 1106 52-53 0.2954565053498257 1106 54-55 0.20652217435307563 1106 56-57 0.26986903992095534 1106 58-59 0.20897449585270778 1106 60-61 0.2123796509442215 1106 62-63 0.4045171446488993 1106 64-65 0.3957789778092575 1106 66-67 0.19864868361693766 1106 68-69 0.33961633269414193 1106 70-71 0.35238118785014194 1106 72-73 0.28491702362792637 1106 74-75 0.12100179207474326 1106 76-77 0.19695706790659528 1106 78-79 0.20115212461725918 1106 80-81 0.14019065515690698 1106 82-83 0.18647949131862518 1106 84-85 0.12895481359458572 1106 86-87 0.13243260576008353 1106 88-89 0.2635119088037676 1106 90-91 0.018494519459501646 1106 92-93 0.12025141511997006 1106 94-95 -0.3162497387383567 1106 96-97 0.16256892312029336 1106 98-99 0.45084872686460997 1106 100-101 0.6705298564819344 1107 1 0.10286922166829271 1107 2 0.14758747228154334 1107 3 0.049855184730397184 1107 4 0.1500549251669625 1107 5 0.06867562185715315 1107 6 0.10555067156416698 1107 7 0.11185210182198091 1107 8 0.06064531633004577 1107 9 0.01897927828589019 1107 10-11 0.10664444428761044 1107 12-13 0.1617595647688077 1107 14-15 0.0946057100843305 1107 16-17 0.11514115296537142 1107 18-19 0.17974943626896334 1107 20-21 0.1844998273686187 1107 22-23 0.14620369077871231 1107 24-25 0.11685948657450496 1107 26-27 0.06177304559319907 1107 28-29 0.10132025765216213 1107 30-31 0.03455221594060731 1107 32-33 -0.0066626291187432685 1107 34-35 0.024708153526972865 1107 36-37 -0.15902749186776077 1107 38-39 -0.049511082963370256 1107 40-41 -0.104961875529618 1107 42-43 -0.03526638752171607 1107 44-45 -0.1341701822877539 1107 46-47 -0.2819941868092073 1107 48-49 -0.5648951672621436 1107 50-51 -0.5587879734019765 1107 52-53 -0.53454776602252 1107 54-55 -0.6230508840473448 1107 56-57 -0.419455463735801 1107 58-59 -0.43703144494150337 1107 60-61 -0.3298629173569836 1107 62-63 -0.5934205324418755 1107 64-65 -0.604728043870395 1107 66-67 -0.46936705198837814 1107 68-69 -0.49474816209380634 1107 70-71 -0.4655874322357434 1107 72-73 -0.4861838818377997 1107 74-75 -0.5590463342274532 1107 76-77 -0.5160450221313262 1107 78-79 -0.24081101115694992 1107 80-81 -0.4322930663496152 1107 82-83 -0.4476122551509718 1107 84-85 -0.40154718677712253 1107 86-87 -0.5889149633073565 1107 88-89 -0.4705674474254238 1107 90-91 -0.39686302049087274 1107 92-93 -0.31988823593173166 1107 94-95 -0.15423511825467884 1107 96-97 -0.103028107134719 1107 98-99 -0.019596474036617906 1107 100-101 0.2738171828962983 1108 1 0.02431377470530549 1108 2 0.047472217773865566 1108 3 0.0068850151243395885 1108 4 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88-89 -0.7166690403017597 2316 90-91 -0.8263573156157982 2316 92-93 -0.8350928956712664 2316 94-95 -0.7344343339480908 2316 96-97 -0.7398135571253164 2316 98-99 -0.5720079286736066 2316 100-101 -0.6111701396035372 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 16.0 5 41.0 6 92.0 7 206.0 8 461.0 9 1011.0 10 1742.0 11 2994.0 12 4838.0 13 6772.0 14 8869.0 15 12704.0 16 17678.0 17 25099.0 18 21948.0 19 12804.0 20 10049.0 21 9646.0 22 9853.0 23 10763.0 24 12021.0 25 14108.0 26 17050.0 27 21112.0 28 26606.0 29 33868.0 30 43274.0 31 54613.0 32 71079.0 33 94224.0 34 130378.0 35 188805.0 36 296810.0 37 481704.0 38 457604.0 39 52993.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.06339544492298 20.102098315967762 15.305967441702734 21.528538797406522 2 15.654036707342723 21.77443430290081 39.410134517447155 23.161394472309315 3 19.252806722111647 25.679029197524244 30.51846301060382 24.549701069760292 4 12.807919968317155 17.7815787286655 37.74204945848057 31.668451844536776 5 13.022843925460622 38.56612608947827 34.2396046509792 14.171425334081903 6 30.619770113661744 37.6698994868252 17.636675214163308 14.073655185349748 7 27.553257032649025 33.983936649167745 20.920706066865474 17.54210025131776 8 26.431636044714413 34.664161381936594 20.017949278662783 18.886253294686206 9 26.60890026078918 16.587250626437253 20.728073999685215 36.07577511308835 10-11 24.401988627790182 27.477090991142827 27.551585604734225 20.569334776332767 12-13 25.872824699609943 24.94603681057934 27.99028500085663 21.190853488954087 14-15 22.555316138982946 26.19505935194097 27.18345360650037 24.066170902575717 16-17 21.140266694895228 29.326131336162693 27.839767634377 21.693834334565082 18-19 21.080861361090182 27.66668198808922 30.76711112720634 20.48534552361426 20-21 22.38928763277986 26.42286104816173 30.824961104479563 20.362890214578844 22-23 21.787221733944524 26.53474347313552 30.80044887500354 20.877585917916416 24-25 21.538217609589555 26.664634640353395 30.959561360888106 20.837586389168944 26-27 21.289398792984855 26.902991909047415 30.89026942990507 20.917339868062662 28-29 21.128335918331246 27.471690964718437 30.671377468429245 20.728595648521072 30-31 21.483443345790114 26.942512629727183 30.65879331261691 20.91525071186579 32-33 21.28449699537618 26.936291203599882 30.637180821107535 21.142030979916402 34-35 21.713659326776693 27.039316163125793 30.545646928495852 20.701377581601665 36-37 21.76345395007166 26.855366235391838 30.466741602351515 20.914438212184987 38-39 21.4512219298771 27.20968573905203 30.43978982722543 20.899302503845448 40-41 21.881869974979654 27.088507215229306 30.174682788517476 20.854940021273563 42-43 21.515882997423123 27.18809849568845 30.00448323985705 21.291535267031378 44-45 21.793934295240096 27.308903557216457 29.77521151688119 21.121950630662255 46-47 22.088152036797414 27.638221284984493 29.13907069536508 21.134555982853005 48-49 22.01925206386526 27.73539624680862 28.886313651805352 21.359038037520772 50-51 22.048478838097598 28.14187822997641 28.73342442615469 21.0762185057713 52-53 22.27586267733966 28.160844293954028 28.301128167413935 21.262164861292376 54-55 22.12487702236974 28.119685381552173 28.32766208556132 21.427775510516764 56-57 22.168450219537412 28.28086210394703 28.143967514869907 21.40672016164565 58-59 22.323405515804975 28.081938967809688 28.13800144578515 21.456654070600187 60-61 22.002468141887515 28.215119272631718 28.646069103329996 21.136343482150775 62-63 21.881173546681822 28.042033626343933 28.785331548619748 21.291461278354497 64-65 21.93619138221053 28.274385320777196 28.438603113405918 21.350820183606356 66-67 21.860977126044702 28.232878194226423 28.28148888941508 21.624655790313795 68-69 21.997036001164428 28.15726929535849 28.31498709054079 21.530707612936297 70-71 22.122532380433707 28.023137205196097 28.174958574123416 21.679371840246777 72-73 22.064793023446928 27.93376768873126 28.289875353031768 21.71156393479005 74-75 21.85178427251332 28.39138527481293 27.9527747328385 21.80405571983525 76-77 21.926928885849367 27.963801514220833 28.2971353118397 21.8121342880901 78-79 21.86859140876766 28.164906792358046 27.986226505411015 21.98027529346328 80-81 22.054751812629174 28.248531174343206 27.927733011614336 21.768984001413287 82-83 21.86881482894977 28.231703268718533 28.159088809248225 21.740393093083473 84-85 21.6851102237486 28.353785721067677 27.9604619412872 22.000642113896518 86-87 21.626327218228592 28.362228143410373 27.874844406311126 22.136600232049908 88-89 21.820978927472563 28.242604976119473 27.577051666158276 22.359364430249688 90-91 21.774467839111164 28.03050265630626 27.733541476243683 22.461488028338884 92-93 21.636721097749607 27.919707478487688 27.967459744282785 22.47611167947992 94-95 21.513993333788335 27.939472952349913 27.97338901045432 22.57314470340744 96-97 21.233123801272793 27.948271163179182 28.169735361889682 22.648869673658343 98-99 21.23374852153767 28.17478529651077 27.765748888324005 22.82571729362755 100-101 20.868078522638072 28.56437060720084 27.82774202490672 22.739808845254366 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 420.0 1 307.0 2 239.0 3 306.0 4 295.0 5 303.5 6 519.0 7 1018.0 8 1695.0 9 2133.0 10 2164.0 11 2165.0 12 2381.0 13 2691.0 14 2975.0 15 3217.5 16 3467.0 17 3752.5 18 4109.5 19 4690.0 20 5258.5 21 5764.5 22 6507.5 23 7648.5 24 9192.0 25 11122.0 26 13747.5 27 17254.5 28 20980.0 29 25216.5 30 29810.0 31 34267.5 32 38926.0 33 43076.5 34 47492.5 35 52380.0 36 58027.0 37 62548.0 38 65359.0 39 68198.5 40 70814.0 41 73809.5 42 75423.5 43 76479.5 44 78799.0 45 81131.5 46 82888.5 47 86304.5 48 90989.0 49 88997.5 50 81559.5 51 75232.0 52 70197.5 53 66982.0 54 64066.0 55 58248.5 56 50435.5 57 43421.5 58 38759.5 59 33836.5 60 26622.5 61 20750.5 62 16361.5 63 11701.0 64 7858.0 65 5476.5 66 4079.5 67 3173.5 68 2355.5 69 1731.0 70 1260.5 71 777.5 72 561.5 73 487.5 74 329.0 75 213.5 76 143.0 77 72.0 78 47.0 79 25.5 80 11.0 81 7.5 82 6.0 83 3.5 84 1.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05645712067755973 2 0.0 3 0.0 4 0.0 5 0.01894284970102333 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002971427404082091 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.357139573980882E-4 24-25 1.6249993616073935E-4 26-27 9.982138935588274E-4 28-29 0.0034124986593755268 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012558923637565715 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.857142127551307E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.3214276594391337E-5 82-83 2.7857131913269605E-4 84-85 0.0010910709999363928 86-87 0.0 88-89 0.0 90-91 0.009216067807973361 92-93 0.0010446424467476102 94-95 0.0 96-97 0.0 98-99 2.0892848934952205E-4 100-101 0.011676781126978843 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2153847.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.89473059091418 #Duplication Level Percentage of deduplicated Percentage of total 1 73.79429739870993 41.985066696454005 2 14.740186749054418 16.772779078944286 3 5.188810917849693 8.856479977747574 4 2.3095853803732878 5.256129519722091 5 1.226863243195968 3.4901026846764918 6 0.7323581550810742 2.5000391957637995 7 0.45295099001463546 1.8039367183439379 8 0.31870897769170564 1.4506289138140216 9 0.21441557847587742 1.0979204914692056 >10 0.9428075391145747 9.141864874894337 >50 0.049345872961328086 1.9295571567786038 >100 0.026557111397576057 2.890901020000738 >500 0.002129322054738001 0.8198870049785102 >1k 7.370730189477169E-4 0.7207326774839106 >5k 1.6379400421060372E-4 0.7186824940206772 >10k+ 8.189700210530186E-5 0.5652914949078616 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12132 0.5632712072863114 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9209 0.4275605463154997 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6215 0.2885534580682843 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4083 0.18956778266979968 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.6428553188782674E-5 0.0 18-19 0.0 0.0 0.0 4.6428553188782674E-5 0.0 20-21 0.0 0.0 0.0 6.964282978317401E-5 0.0 22-23 0.0 0.0 0.0 9.285710637756535E-5 0.0 24-25 0.0 0.0 0.0 3.714284255102614E-4 0.0 26-27 0.0 0.0 0.0 6.732140212373488E-4 0.0 28-29 0.0 0.0 0.0 0.0018803564041456984 0.0 30-31 0.0 0.0 0.0 0.00503749802098292 0.0 32-33 0.0 0.0 0.0 0.007683925552743533 0.0 34-35 0.0 0.0 0.0 0.010167853148343405 0.0 36-37 0.0 0.0 0.0 0.013905351680040413 0.0 38-39 0.0 0.0 0.0 0.0201035635307429 0.0 40-41 0.0 0.0 0.0 0.027648203423920083 0.0 42-43 0.0 0.0 0.0 0.034728557785209446 0.0 44-45 0.0 0.0 0.0 0.03841962776371766 0.0 46-47 0.0 0.0 0.0 0.04382855421021084 0.0 48-49 0.0 0.0 0.0 0.05329997906072251 0.0 50-51 0.0 0.0 0.0 0.06286426101761175 0.0 52-53 0.0 0.0 0.0 0.08626425182475822 0.0 54-55 0.0 0.0 0.0 0.1007499604196584 0.0 56-57 0.0 0.0 0.0 0.14787494190627282 0.0 58-59 0.0 0.0 0.0 0.2360891929649599 0.0 60-61 0.0 0.0 0.0 0.3142980908114643 0.0 62-63 0.0 0.0 0.0 0.3553641461069426 0.0 64-65 0.0 0.0 0.0 0.42967304548558927 0.0 66-67 0.0 0.0 0.0 0.5811926288171816 0.0 68-69 0.0 0.0 0.0 0.7935336168260791 0.0 70-71 0.0 0.0 0.0 0.989160325687015 0.0 72-73 0.0 0.0 0.0 1.218795949758734 0.0 74-75 0.0 0.0 0.0 1.5062119082738932 0.0 76-77 0.0 0.0 0.0 1.7111475420491797 0.0 78-79 0.0 0.0 0.0 1.9910188606711618 0.0 80-81 0.0 0.0 0.0 2.2878830297602386 0.0 82-83 0.0 0.0 0.0 2.5098579425558083 0.0 84-85 0.0 0.0 0.0 2.84428281117461 0.0 86-87 0.0 0.0 0.0 3.3091022714241074 0.0 88-89 0.0 0.0 0.0 3.9702448688323733 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14450 0.0 31.611773 1 GGTATCA 9740 0.0 31.135443 1 TATCAAC 17900 0.0 25.453474 2 ATCAACG 17900 0.0 25.267681 3 TCAACGC 17900 0.0 25.267681 4 CAACGCA 18130 0.0 24.947132 5 TACATGG 10185 0.0 24.722725 2 GTACATG 10480 0.0 24.628157 1 AACGCAG 18625 0.0 24.252405 6 ACATGGG 10250 0.0 23.221771 3 GAGTACT 10785 0.0 22.616278 12-13 CATGGGT 3240 0.0 22.141827 4 GGACCGA 430 0.0 22.091852 6 AGTACTT 11110 0.0 21.527134 12-13 AGAGTAC 16110 0.0 21.524256 10-11 ACGCAGA 20985 0.0 21.32273 7 CGCAGAG 20900 0.0 21.27308 8 GTACTTT 11485 0.0 21.050255 14-15 GCAGAGT 21780 0.0 19.999186 9 GTATAGC 1075 0.0 19.89745 1 >>END_MODULE