FastQCFastQC Report
Thu 26 May 2016
SRR937436_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937436_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293710
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107010.46653674614489193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74530.32493209690850194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52580.22923560519856478No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34370.1498445749462661No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32700.14256379402801575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA140950.033.9467771
GGTATCA90400.031.0637021
ATCAACG167900.028.2653263
TCAACGC167850.028.2208424
CAACGCA169350.027.9440465
TATCAAC170750.027.9042222
AACGCAG173450.027.3091096
GTACATG109450.025.1794591
TAGATCG1151.2802414E-424.7884624
TACATGG107900.024.7865832
TAGCCCT18950.024.3197254
ACGCAGA192050.024.3178887
CGCAGAG191650.024.1212718
CATGGGT33100.023.9709174
GCGTATC1201.7077872E-423.7576851
ACATGGG107000.023.2197283
GAGTACT95750.023.02044112-13
GCAGAGT201200.022.2915469
AGTACTT98150.021.94933912-13
AGAGTAC152300.021.7718210-11
Produced by FastQC (version 0.11.5)