##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937436_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2293710 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.838305191153196 33.0 31.0 34.0 30.0 34.0 2 31.932920029123125 33.0 31.0 34.0 30.0 34.0 3 31.980453065121573 33.0 31.0 34.0 30.0 34.0 4 35.59110872778163 37.0 35.0 37.0 33.0 37.0 5 35.61101316208239 37.0 35.0 37.0 33.0 37.0 6 35.64065248004325 37.0 35.0 37.0 33.0 37.0 7 35.53444986506577 37.0 35.0 37.0 33.0 37.0 8 35.543592258829584 37.0 35.0 37.0 33.0 37.0 9 37.18760087369371 39.0 37.0 39.0 34.0 39.0 10-11 37.079678119727426 39.0 37.0 39.0 33.0 39.0 12-13 36.94635197998004 39.0 37.0 39.0 33.0 39.0 14-15 38.13951872730206 40.0 38.0 41.0 33.0 41.0 16-17 38.08307567216431 40.0 38.0 41.0 33.0 41.0 18-19 38.07005920539214 40.0 38.0 41.0 33.0 41.0 20-21 37.96531623439755 40.0 38.0 41.0 33.0 41.0 22-23 37.814257251352615 40.0 37.5 41.0 33.0 41.0 24-25 37.75957597080712 40.0 37.5 41.0 32.5 41.0 26-27 37.78618024946484 40.0 37.5 41.0 33.0 41.0 28-29 37.707445579432445 40.0 37.5 41.0 33.0 41.0 30-31 37.505801518064615 40.0 37.0 41.0 32.0 41.0 32-33 37.262193346150994 39.5 36.5 41.0 32.0 41.0 34-35 37.289375945520575 40.0 37.0 41.0 32.0 41.0 36-37 37.17252159165719 40.0 36.5 41.0 31.5 41.0 38-39 37.06092487716407 40.0 36.0 41.0 31.0 41.0 40-41 36.84519642849358 39.5 36.0 41.0 30.5 41.0 42-43 36.57628863282629 39.0 36.0 41.0 30.0 41.0 44-45 36.329026773218935 39.0 36.0 41.0 30.0 41.0 46-47 35.97162936901352 39.0 35.0 41.0 28.5 41.0 48-49 35.63006897123002 39.0 35.0 41.0 27.5 41.0 50-51 34.64691155377096 38.0 34.0 39.5 25.5 40.5 52-53 34.75578996472963 38.0 34.5 39.5 26.0 40.5 54-55 35.14336838571572 39.0 35.0 40.0 26.0 41.0 56-57 34.93383034472536 38.5 34.5 40.0 25.5 41.0 58-59 34.680375461588426 38.0 34.0 40.0 25.0 41.0 60-61 34.38283784785348 38.0 34.0 40.0 23.5 41.0 62-63 34.086304720300305 38.0 34.0 40.0 23.0 41.0 64-65 33.68650417881947 37.0 33.5 40.0 22.0 41.0 66-67 33.34271224348326 37.0 33.0 39.5 21.5 41.0 68-69 32.87143819401756 36.0 32.5 39.0 19.5 40.5 70-71 32.41019592712244 35.5 32.0 39.0 18.0 40.0 72-73 31.95954915835044 35.0 31.5 38.0 15.5 40.0 74-75 31.48728566383719 35.0 31.0 37.5 12.0 39.5 76-77 31.005919885251405 35.0 31.0 37.0 7.5 39.0 78-79 30.513332548578504 34.5 30.0 36.0 7.0 39.0 80-81 30.03001229449233 34.0 30.0 36.0 2.0 37.5 82-83 29.562487629212065 34.0 29.0 35.0 2.0 37.0 84-85 29.05201551198713 34.0 29.0 35.0 2.0 36.5 86-87 28.611753447471564 34.0 29.0 35.0 2.0 36.0 88-89 28.168319447532603 34.0 27.5 35.0 2.0 36.0 90-91 27.677877761356058 33.5 26.5 35.0 2.0 35.0 92-93 27.199557049496228 33.0 26.0 35.0 2.0 35.0 94-95 26.697839090381958 33.0 25.0 35.0 2.0 35.0 96-97 26.008861189949908 32.5 24.5 35.0 2.0 35.0 98-99 25.130866587319233 32.0 19.0 34.0 2.0 35.0 100-101 22.550044469440337 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08075360939793796 1101 2 -0.1341503329769438 1101 3 -0.08175609929961425 1101 4 -0.10018557075241574 1101 5 -0.10873838130197555 1101 6 -0.08841132489772008 1101 7 -0.11808842595857527 1101 8 -0.21263254084547611 1101 9 -0.16596283366891385 1101 10-11 -0.22133072381691932 1101 12-13 -0.11588266274073789 1101 14-15 -0.1496011311353982 1101 16-17 -0.26114939726629416 1101 18-19 -0.09860736274980297 1101 20-21 0.05815428024113345 1101 22-23 -0.08549023977745662 1101 24-25 -0.15361668701699216 1101 26-27 -0.2227572829820943 1101 28-29 -0.2317217161389422 1101 30-31 -0.18231281251682674 1101 32-33 -0.2882474452258066 1101 34-35 -0.20337878820142663 1101 36-37 -0.10799416547173024 1101 38-39 -0.15093112352970905 1101 40-41 -0.3218418939130814 1101 42-43 -0.15431557084479408 1101 44-45 -0.13338872611159047 1101 46-47 -0.0525618695429273 1101 48-49 -0.1832576615661239 1101 50-51 -0.17733562377569 1101 52-53 -0.0646377736656305 1101 54-55 -0.11708902855408354 1101 56-57 -0.047528102432984554 1101 58-59 0.012933553866417924 1101 60-61 0.14278676365399434 1101 62-63 0.0019134339163571212 1101 64-65 -0.07094717677478712 1101 66-67 -0.1672973504591866 1101 68-69 -0.1642511642567399 1101 70-71 -0.1437938969404513 1101 72-73 -1.99893846779986E-4 1101 74-75 -0.10504586169740548 1101 76-77 -0.03638608062033555 1101 78-79 -0.20153487277214666 1101 80-81 -0.10866836920134304 1101 82-83 0.014990960548320942 1101 84-85 0.008089862573356754 1101 86-87 0.10244830568523255 1101 88-89 8.331319131649195E-4 1101 90-91 -0.03850732563685 1101 92-93 -0.0832363178674349 1101 94-95 -0.19619487044822037 1101 96-97 -0.12278543990605684 1101 98-99 -0.04370459210856836 1101 100-101 0.29132735283182143 1102 1 -0.05442033447752692 1102 2 -0.040125188461132666 1102 3 0.02095094642197637 1102 4 -0.14395987670581434 1102 5 0.03356279571838172 1102 6 -0.09615637271767952 1102 7 0.0466225311820736 1102 8 -0.04524048680004711 1102 9 -0.12371821608258671 1102 10-11 -0.011706127933237553 1102 12-13 0.03766707361479149 1102 14-15 0.08492829100354271 1102 16-17 0.22738201203528519 1102 18-19 0.05620091930372695 1102 20-21 0.04268847651304242 1102 22-23 -0.0961427345708401 1102 24-25 -0.1280701611254642 1102 26-27 -0.12261029585315697 1102 28-29 -0.14628116476590236 1102 30-31 -0.19014281182037251 1102 32-33 -0.20694662879290604 1102 34-35 -0.0301377192000416 1102 36-37 -0.01211609570423633 1102 38-39 0.0323054353201897 1102 40-41 -0.11294366831788949 1102 42-43 -0.12792332134074513 1102 44-45 0.10199386167067104 1102 46-47 0.10235088177304874 1102 48-49 0.06902942895367659 1102 50-51 -0.07186547839491197 1102 52-53 0.07601239584417385 1102 54-55 0.06467049780699341 1102 56-57 -0.025367279305065438 1102 58-59 -0.056646064068146984 1102 60-61 0.14765202638857744 1102 62-63 0.03261349592391838 1102 64-65 0.05271367892957102 1102 66-67 -0.11563814267318406 1102 68-69 -0.19459639585273436 1102 70-71 -0.2026562884553016 1102 72-73 -0.17422462273219352 1102 74-75 -0.15474290083630748 1102 76-77 0.006767307216744456 1102 78-79 0.005673450170426975 1102 80-81 -0.08439021810509217 1102 82-83 -0.005617707704480779 1102 84-85 -0.08768472084141621 1102 86-87 -0.013424815833861459 1102 88-89 -0.12054835613353276 1102 90-91 -0.13513788300845064 1102 92-93 -0.10925042795782147 1102 94-95 0.11930974417252926 1102 96-97 0.13303043695051642 1102 98-99 0.18468880543404254 1102 100-101 0.4982228296497553 1103 1 0.10648037097763563 1103 2 -0.010464530087602952 1103 3 -0.048309825809095486 1103 4 -0.016474051200184192 1103 5 -0.07552503614172679 1103 6 -0.05675627886213874 1103 7 0.0018103576570283053 1103 8 0.024293389664521214 1103 9 0.006571735989545857 1103 10-11 0.06612088553734452 1103 12-13 0.04841045564530333 1103 14-15 -0.09707837120112828 1103 16-17 -0.09474058483978354 1103 18-19 -0.08965982061642563 1103 20-21 -0.0191465531437629 1103 22-23 -0.12199724309861892 1103 24-25 -0.09900186968093294 1103 26-27 -0.19175374865996986 1103 28-29 -0.1416364695271497 1103 30-31 -0.14803667813007593 1103 32-33 -0.12240548237056714 1103 34-35 -0.06782610342308004 1103 36-37 -0.00840226278248224 1103 38-39 -0.2251991612933253 1103 40-41 -0.08435427091661296 1103 42-43 -0.12341517821874248 1103 44-45 -0.17968430614610753 1103 46-47 0.07778688691202973 1103 48-49 0.03816865056814578 1103 50-51 -0.002979743667594903 1103 52-53 -0.0964183087647541 1103 54-55 0.056008093104985335 1103 56-57 0.006251267164969931 1103 58-59 -0.021800271796607262 1103 60-61 0.0034376049464270864 1103 62-63 0.11418703789350104 1103 64-65 -0.009061729380483996 1103 66-67 0.09237304306076766 1103 68-69 0.1944713570764094 1103 70-71 0.1587529293895571 1103 72-73 0.023487456168002296 1103 74-75 0.2740961369136201 1103 76-77 0.44910961837093666 1103 78-79 0.3470268162762693 1103 80-81 0.2636465086445057 1103 82-83 0.3731913270451521 1103 84-85 0.23395513545190383 1103 86-87 0.4846557614546363 1103 88-89 0.5696148909604091 1103 90-91 0.5766525268017624 1103 92-93 0.5205375508400607 1103 94-95 0.3020934071542136 1103 96-97 0.2469341350126335 1103 98-99 0.546319199242653 1103 100-101 1.048087431494558 1104 1 0.0525237329923911 1104 2 0.012736676069504682 1104 3 -0.030928716652063315 1104 4 -0.028535622260562832 1104 5 -0.09809834417391983 1104 6 -0.07050521037030677 1104 7 -0.022009022538995282 1104 8 -0.07723949673129482 1104 9 0.011385621613918318 1104 10-11 0.04807668632508921 1104 12-13 0.0038067677162203495 1104 14-15 0.10862193432038936 1104 16-17 0.03357567577683085 1104 18-19 0.08311357206162029 1104 20-21 0.1579867029525417 1104 22-23 -0.08026910631684814 1104 24-25 0.04368172800862169 1104 26-27 0.1604128240071958 1104 28-29 0.11047018424816457 1104 30-31 0.10139052689903139 1104 32-33 0.14701467501144805 1104 34-35 0.29728901586367584 1104 36-37 0.18303743908629855 1104 38-39 0.25345137635602555 1104 40-41 0.3374147625925943 1104 42-43 0.4428709062437406 1104 44-45 0.40542807187448915 1104 46-47 0.49350013009844673 1104 48-49 0.5591620261503181 1104 50-51 0.3518570167114774 1104 52-53 0.24071019642096303 1104 54-55 0.34772743190418254 1104 56-57 0.4304721595026635 1104 58-59 0.4715815615733021 1104 60-61 0.4836773360800848 1104 62-63 0.6882074751439688 1104 64-65 0.7552741201758622 1104 66-67 0.6047048047965831 1104 68-69 0.4289227750796698 1104 70-71 0.4964447369860139 1104 72-73 0.6932250818055117 1104 74-75 0.647560310139415 1104 76-77 0.5356914910076362 1104 78-79 0.577768958520199 1104 80-81 0.6440393246749885 1104 82-83 0.6499126523362015 1104 84-85 0.8021124118928498 1104 86-87 0.6590903521585965 1104 88-89 0.5708947658993999 1104 90-91 0.5325693089998325 1104 92-93 0.5791499422891597 1104 94-95 0.7310184418599412 1104 96-97 0.8321495420397191 1104 98-99 0.7511804550574261 1104 100-101 0.8778781876857877 1105 1 -0.035064416584852154 1105 2 -0.028022684813898024 1105 3 -0.028424106808330407 1105 4 -0.14302547421178247 1105 5 -0.015348732602596726 1105 6 -0.11204366600793492 1105 7 -0.0577505337622739 1105 8 -0.16754672695435602 1105 9 -0.04243775992260623 1105 10-11 -0.15432214242780873 1105 12-13 -0.17127619988206533 1105 14-15 -0.17754092732740645 1105 16-17 -0.1857940741233648 1105 18-19 -0.07467333217860528 1105 20-21 -0.02814566612517666 1105 22-23 -0.06195846182090037 1105 24-25 -0.040231731264775306 1105 26-27 -0.044794022054226446 1105 28-29 -0.1638583479437159 1105 30-31 -0.04060256848357113 1105 32-33 -0.1163682550944003 1105 34-35 -0.02519764044063777 1105 36-37 0.014759924096516386 1105 38-39 -0.011852282017237314 1105 40-41 0.06946609750553279 1105 42-43 0.04905664690794964 1105 44-45 0.08190022534993346 1105 46-47 0.008580527882656952 1105 48-49 0.15671283990879914 1105 50-51 -0.18035251838550437 1105 52-53 -0.006009455081567694 1105 54-55 0.10865849088812496 1105 56-57 0.013496971313330164 1105 58-59 0.1941818778138611 1105 60-61 0.2964305019216269 1105 62-63 0.31089372353824274 1105 64-65 0.19921573553414618 1105 66-67 0.13348704213937168 1105 68-69 0.11628651340483032 1105 70-71 -0.0424094775250623 1105 72-73 0.12580573768982717 1105 74-75 0.21273370386976964 1105 76-77 0.23081021865560913 1105 78-79 0.31559438440136134 1105 80-81 0.4616252752073464 1105 82-83 0.40251107317905266 1105 84-85 0.33362955149799944 1105 86-87 0.4074731112631369 1105 88-89 0.3751291898375406 1105 90-91 0.39463493770452374 1105 92-93 0.5587085735243562 1105 94-95 0.5322678541428196 1105 96-97 0.7078829698552731 1105 98-99 0.7545657216484685 1105 100-101 1.1629082056073656 1106 1 -0.20535504929151926 1106 2 -0.0950482863581037 1106 3 -0.25943203693130457 1106 4 -0.09988093882394367 1106 5 -0.0908514099018376 1106 6 -0.2501880144625517 1106 7 -0.4202337963148395 1106 8 -0.25871510117529084 1106 9 -0.4414675072304206 1106 10-11 -0.7755526684856093 1106 12-13 -0.3721920342342955 1106 14-15 -0.48633904359433444 1106 16-17 -0.40958638161329475 1106 18-19 -0.5167051648304408 1106 20-21 -0.522735333840977 1106 22-23 -0.5905071468494825 1106 24-25 -0.4907556981817436 1106 26-27 -0.7084497367415068 1106 28-29 -0.37839469122513236 1106 30-31 -0.4140098281393705 1106 32-33 -0.2724595782078012 1106 34-35 -0.16648246713968717 1106 36-37 -0.5703384858632816 1106 38-39 -0.33147123593889205 1106 40-41 -0.27353456853064273 1106 42-43 -0.16141666802746357 1106 44-45 -0.15742259864343566 1106 46-47 -0.26009427476432023 1106 48-49 0.028717927262000842 1106 50-51 -0.01739053368079624 1106 52-53 0.1306788912240151 1106 54-55 -0.08760097520288213 1106 56-57 -0.13824780805699532 1106 58-59 0.08850575417206841 1106 60-61 -0.027748411179345567 1106 62-63 -0.041421409883625415 1106 64-65 -0.008296764760096664 1106 66-67 -3.190707156832673E-4 1106 68-69 -0.2017786445952794 1106 70-71 -0.07511811924052125 1106 72-73 -0.0555210520573759 1106 74-75 0.28717726595860427 1106 76-77 0.3700576462466749 1106 78-79 0.21565918792068928 1106 80-81 -0.22227703902602514 1106 82-83 -0.06495302525582858 1106 84-85 -0.08348027385097367 1106 86-87 0.029455005049062066 1106 88-89 0.19381365070133683 1106 90-91 0.3649684612020323 1106 92-93 0.4040176918503242 1106 94-95 0.5575670165434872 1106 96-97 0.6412127145321769 1106 98-99 0.8084613409028698 1106 100-101 0.976850101221082 1107 1 0.038330467122687395 1107 2 0.11714905224784289 1107 3 0.0060148921637477315 1107 4 0.0999665012041433 1107 5 0.1633836044486685 1107 6 0.21508241859104515 1107 7 0.21521819460949132 1107 8 0.18002173491421303 1107 9 0.13237248404126234 1107 10-11 0.12246399621422199 1107 12-13 0.14415871342460918 1107 14-15 0.29621846420436526 1107 16-17 0.08019013342857306 1107 18-19 0.1824176060534981 1107 20-21 0.26821130245470926 1107 22-23 0.21828160267828167 1107 24-25 0.2603622461514874 1107 26-27 0.1621369344674477 1107 28-29 0.1712696842586965 1107 30-31 0.14200901715494751 1107 32-33 0.22942710361466823 1107 34-35 0.13924033165111638 1107 36-37 0.05548795796413941 1107 38-39 0.19351328911383092 1107 40-41 0.26983369290912407 1107 42-43 0.4415646529318522 1107 44-45 0.3967067815645109 1107 46-47 0.46615233058147965 1107 48-49 0.3951834196139643 1107 50-51 0.31126756572848535 1107 52-53 0.25508206666835065 1107 54-55 0.24348440865374243 1107 56-57 0.2323909101868864 1107 58-59 0.23334773529188624 1107 60-61 0.3322406116278387 1107 62-63 0.41343813177590505 1107 64-65 0.41216868602845835 1107 66-67 0.40301780292933387 1107 68-69 0.26132659469490704 1107 70-71 0.390703492840764 1107 72-73 0.5245318336677478 1107 74-75 0.5814607573442174 1107 76-77 0.4168193558090074 1107 78-79 0.4550844550583584 1107 80-81 0.4627486857544483 1107 82-83 0.6371014502696326 1107 84-85 0.5833323286195231 1107 86-87 0.5349816394001792 1107 88-89 0.7019955188132236 1107 90-91 0.6648056773740585 1107 92-93 0.8288221582837885 1107 94-95 0.9417376855436395 1107 96-97 0.8315307228433717 1107 98-99 1.132223771887201 1107 100-101 1.5642941297840522 1108 1 -0.001180029458431875 1108 2 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-0.18955461795513173 2316 14-15 -0.22629016615878328 2316 16-17 -0.3022802827109814 2316 18-19 -0.2125718993296033 2316 20-21 -0.25384049640124573 2316 22-23 -0.2141877413664659 2316 24-25 -0.09903728546220236 2316 26-27 -0.12586644382238887 2316 28-29 -0.07507089881793405 2316 30-31 -0.11939926275233148 2316 32-33 -0.11720794816751123 2316 34-35 -0.28235558867080357 2316 36-37 -0.4619764663566812 2316 38-39 -0.21588695722973483 2316 40-41 -0.3434432482376337 2316 42-43 -0.2336376751815763 2316 44-45 -0.38831974682575066 2316 46-47 -0.2993471822109939 2316 48-49 -0.31145808856135915 2316 50-51 -0.6222214595943925 2316 52-53 -0.5565562371347283 2316 54-55 -0.46995596738945977 2316 56-57 -0.3938923277300077 2316 58-59 -0.5150798589988455 2316 60-61 -0.4941562683380454 2316 62-63 -0.39063594759247167 2316 64-65 -0.39549512410117416 2316 66-67 -0.48711392178531554 2316 68-69 -0.2356608189121232 2316 70-71 -0.22097185779379203 2316 72-73 -0.3007161180355702 2316 74-75 -0.2606220607880019 2316 76-77 -0.2587230645998666 2316 78-79 -0.12038744533688828 2316 80-81 -0.03941813696157226 2316 82-83 -0.3392368218470878 2316 84-85 -0.13993141384901264 2316 86-87 -0.2446635686934222 2316 88-89 -0.3532388053739517 2316 90-91 -0.268961496989057 2316 92-93 -0.20316676816812773 2316 94-95 -0.023183372542451508 2316 96-97 0.11131530326120398 2316 98-99 0.134830749080173 2316 100-101 -0.10031649729611303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8027.0 3 1696.0 4 1200.0 5 1281.0 6 1616.0 7 1903.0 8 2457.0 9 3022.0 10 3975.0 11 5347.0 12 7599.0 13 10269.0 14 11823.0 15 15204.0 16 19441.0 17 26063.0 18 21927.0 19 14631.0 20 13968.0 21 14655.0 22 15790.0 23 17665.0 24 19572.0 25 22347.0 26 26240.0 27 30692.0 28 36553.0 29 43990.0 30 53965.0 31 67326.0 32 85825.0 33 112239.0 34 152868.0 35 215822.0 36 321950.0 37 459953.0 38 380057.0 39 44750.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.299388592538975 19.195038455553632 15.034600382195226 22.470972569712163 2 14.836211382945114 20.597423060839517 40.746610595714536 23.819754960500827 3 18.74206169756788 25.18249084105044 30.575839597683448 25.499607863698227 4 12.063817069270174 16.814311256993346 38.70324442323792 32.41862725049855 5 12.878906053714179 37.85903578396352 34.894553208409526 14.367504953912771 6 30.56855859848614 36.77632056281541 18.382543841015835 14.272576997682618 7 27.294199921325234 33.33602867258184 21.567376196512086 17.802395209580837 8 25.981130822104593 33.98165176105334 20.77641754830048 19.260799868541582 9 26.15467511775086 16.342219965336806 21.147302851978715 36.35580206493362 10-11 24.029168592410002 26.767777752283305 28.427074694803828 20.775978960502865 12-13 25.400492870596448 24.36547335922321 28.556993161567984 21.677040608612362 14-15 22.288143111037694 25.6040053331026 27.568587658613264 24.539263897246443 16-17 21.06272756952794 28.699120687296574 28.200134001329523 22.03801774184596 18-19 20.86001891798644 27.29045183571986 30.89188320492278 20.957646041370914 20-21 22.233183013904547 26.142099927462137 30.772921528014475 20.851795530618844 22-23 21.510007799988202 26.281590498434543 30.74108627398715 21.46731542759011 24-25 21.275570764434033 26.326704100432778 30.98803163802112 21.409693497112066 26-27 21.074057307061043 26.567724619872717 30.810126880972398 21.54809119209384 28-29 20.920372167909978 27.101726448981626 30.627479396724016 21.350421986384376 30-31 21.300857352264398 26.56705961432478 30.643208683570716 21.488874349840106 32-33 20.997340800390894 26.630893085928047 30.61672059436356 21.75504551931749 34-35 21.5145839083655 26.68514199934394 30.540529141045635 21.259744951244922 36-37 21.381480126065796 26.56267512931576 30.48559365082833 21.570251093790112 38-39 21.154223459913105 26.88562026243494 30.408356423512263 21.55179985413969 40-41 21.614146778948932 26.707594564746007 30.253377917068576 21.42488073923649 42-43 21.191912337113987 26.773008000835503 30.037818198661668 21.997261463388842 44-45 21.498002329941272 26.877408857054757 29.8866868833834 21.737901929620566 46-47 21.652280940384173 27.152111353738512 29.363009830780502 21.832597875096813 48-49 21.569406508254755 27.150058592563354 29.301876491859325 21.978658407322563 50-51 21.68074587726052 27.509152233590832 29.114800731435626 21.695301157713022 52-53 21.765856708170116 27.533685121712363 28.741649762171235 21.95880840794628 54-55 21.671092228713047 27.41614925836322 28.911530282864444 22.00122823005929 56-57 21.733651269318784 27.63923791495868 28.610109368056346 22.01700144766619 58-59 21.86973202507155 27.47784460542984 28.70516466609681 21.947258703401804 60-61 21.639917508045716 27.493757510265077 29.1228010391662 21.743523942523005 62-63 21.403402380888924 27.41856135658327 29.23050809394755 21.947528168580252 64-65 21.405112578296936 27.585904094103768 28.91279345991907 22.096189867680227 66-67 21.38978231788247 27.498040572598427 28.80677206070599 22.305405048813114 68-69 21.58455244752342 27.3995135994883 28.903934260565713 22.11199969242256 70-71 21.617386042538875 27.249652936685386 28.784748656256042 22.3482123645197 72-73 21.455191764410838 27.206157992098913 28.962167041390284 22.376483202099966 74-75 21.370120677752823 27.538405410837402 28.671386876622073 22.4200870347877 76-77 21.44916706813884 27.263358098977363 28.813204556354133 22.474270276529666 78-79 21.380375161837645 27.355707237611092 28.716913131800403 22.54700446875086 80-81 21.52741928189941 27.423651210157075 28.53190503477862 22.517024473164895 82-83 21.466282886113643 27.34547851982342 28.843856714936333 22.3443818791266 84-85 21.4148459977324 27.352458640358446 28.669347879751744 22.563347482157408 86-87 21.34147247327201 27.49048331477769 28.630497436874403 22.5375467750759 88-89 21.496728352191013 27.23101999691088 28.37340412819013 22.898847522707975 90-91 21.394044109196724 27.009224993084864 28.655640022949964 22.94109087476846 92-93 21.39552746811982 26.84716300684311 28.86511972162679 22.892189803410282 94-95 21.161166181980875 26.785654989933448 28.996822744812928 23.056356083272746 96-97 20.93019445122945 26.70369313576367 29.184267020545384 23.181845392461494 98-99 20.994050467244797 26.951645571279688 28.876995649827503 23.17730831164802 100-101 20.89996262461284 26.55022118321607 29.233778350192306 23.316037841978783 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3297.0 1 1946.5 2 543.5 3 456.5 4 396.0 5 408.0 6 573.0 7 992.5 8 1618.0 9 2073.0 10 2110.0 11 2053.5 12 2210.0 13 2433.0 14 2678.0 15 2933.0 16 3171.0 17 3461.0 18 3791.5 19 4210.5 20 4684.0 21 5358.0 22 6348.0 23 7587.5 24 9075.5 25 11135.5 26 13994.5 27 17786.5 28 21711.0 29 25840.5 30 30800.0 31 35919.0 32 41416.0 33 46190.5 34 50636.5 35 55486.5 36 61337.0 37 66397.0 38 69083.5 39 72110.0 40 75266.5 41 78289.0 42 80602.0 43 82093.0 44 84058.0 45 86878.0 46 89799.0 47 93434.5 48 97053.0 49 95010.5 50 88088.5 51 81189.5 52 76008.5 53 72087.5 54 68151.0 55 62065.5 56 54409.5 57 47191.5 58 41686.0 59 35998.0 60 28529.5 61 22372.0 62 17361.5 63 12536.5 64 8635.0 65 6183.0 66 4526.5 67 3294.0 68 2402.5 69 1732.0 70 1255.5 71 855.5 72 565.0 73 423.5 74 317.5 75 233.5 76 182.0 77 115.0 78 67.5 79 46.5 80 28.0 81 22.5 82 17.0 83 8.0 84 6.0 85 6.0 86 4.0 87 4.5 88 4.0 89 3.0 90 3.5 91 2.0 92 2.5 93 2.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.256091659364087 2 0.2258785984278745 3 0.21646154047373034 4 0.2226087866382411 5 0.2230011640529971 6 0.25216788521652694 7 0.2533014199702665 8 0.24126851258441567 9 0.23560083881571775 10-11 0.2455628654014675 12-13 0.2478735323994751 14-15 0.23372614672299463 16-17 0.24732856376786955 18-19 0.2359278199946811 20-21 0.2288868252743372 22-23 0.22866883782169498 24-25 0.2238513151183018 26-27 0.22958438512279233 28-29 0.24427673943087838 30-31 0.2449742992793335 32-33 0.24482170806248393 34-35 0.2526256588670756 36-37 0.22768789428480496 38-39 0.22753530306795539 40-41 0.2237641201372449 42-43 0.22938819641541436 44-45 0.24717597255101997 46-47 0.2526038601218114 48-49 0.2562224518356723 50-51 0.2563750430525219 52-53 0.2768440648556269 54-55 0.255699281949331 56-57 0.22666335325738649 58-59 0.21016170309236998 60-61 0.20750225617013485 62-63 0.21367130107990984 64-65 0.2069790862837935 66-67 0.2082216147638542 68-69 0.2116876152608656 70-71 0.2119927976945647 72-73 0.21415087347572276 74-75 0.21667952792637254 76-77 0.22921380645330056 78-79 0.22457067371202113 80-81 0.2188376037075306 82-83 0.2137148985704383 84-85 0.2155895906631614 86-87 0.22226000671401352 88-89 0.21936077359387193 90-91 0.22901761774592252 92-93 0.21728989279377078 94-95 0.2154587981915761 96-97 0.22526823356047626 98-99 0.23084871234811724 100-101 0.20798182856594777 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2293710.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.938408209086056 #Duplication Level Percentage of deduplicated Percentage of total 1 74.68429543825451 43.27089195909573 2 14.165084120559307 16.414048521860156 3 4.975348230437452 8.647912702723172 4 2.3073980684205977 5.34747884756037 5 1.2068827267805873 3.4962432042354266 6 0.7194748202685266 2.5011135499726027 7 0.44584369472169766 1.8082031787563002 8 0.3106059162855266 1.4396809895926437 9 0.20732716869517961 1.0810985519425826 >10 0.9032251027594804 8.920684744115594 >50 0.04773252546864956 1.8874066365030886 >100 0.02392654255655923 2.6650622416275342 >500 0.0019538622261156186 0.7491905777751645 >1k 6.763369244239129E-4 0.7516288106074213 >5k 1.5029709431642506E-4 0.5534353131918514 >10k+ 7.514854715821253E-5 0.46592017044024875 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10701 0.46653674614489193 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7453 0.32493209690850194 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5258 0.22923560519856478 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3437 0.1498445749462661 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3270 0.14256379402801575 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.3597490528445184E-5 0.0 10-11 0.0 0.0 0.0 4.3597490528445184E-5 0.0 12-13 0.0 0.0 0.0 4.3597490528445184E-5 0.0 14-15 0.0 0.0 0.0 4.3597490528445184E-5 0.0 16-17 0.0 0.0 0.0 4.3597490528445184E-5 0.0 18-19 0.0 0.0 0.0 1.3079247158533555E-4 0.0 20-21 0.0 0.0 0.0 1.3079247158533555E-4 0.0 22-23 0.0 0.0 0.0 1.5259121684955816E-4 0.0 24-25 0.0 0.0 0.0 3.269811789633389E-4 0.0 26-27 0.0 0.0 0.0 5.449686316055648E-4 0.0 28-29 0.0 0.0 0.0 0.0015041134232313589 0.0 30-31 0.0 0.0 0.0 0.004141761600202292 0.0 32-33 0.0 0.0 0.0 0.0068884035034943395 0.0 34-35 0.0 0.0 0.0 0.009809435368900166 0.0 36-37 0.0 0.0 0.0 0.013907599478574013 0.0 38-39 0.0 0.0 0.0 0.021144782906295914 0.0 40-41 0.0 0.0 0.0 0.028665350022452706 0.0 42-43 0.0 0.0 0.0 0.03653469706283706 0.0 44-45 0.0 0.0 0.0 0.0407200561535678 0.0 46-47 2.1798745264222592E-5 0.0 0.0 0.04793544083602548 0.0 48-49 4.3597490528445184E-5 0.0 0.0 0.06016453692925435 0.0 50-51 4.3597490528445184E-5 0.0 0.0 0.07398494142677148 0.0 52-53 4.3597490528445184E-5 0.0 0.0 0.10297727262818751 0.0 54-55 4.3597490528445184E-5 0.0 0.0 0.12359888564814209 0.0 56-57 4.3597490528445184E-5 0.0 0.0 0.17877150991188945 0.0 58-59 4.3597490528445184E-5 0.0 0.0 0.27477318405552575 0.0 60-61 4.3597490528445184E-5 0.0 0.0 0.36288371241351347 0.0 62-63 4.3597490528445184E-5 0.0 0.0 0.41360939264335944 0.0 64-65 4.3597490528445184E-5 0.0 0.0 0.4988860841169982 0.0 66-67 4.3597490528445184E-5 0.0 0.0 0.6598480191480178 0.0 68-69 4.3597490528445184E-5 0.0 0.0 0.8862715862074979 0.0 70-71 4.3597490528445184E-5 0.0 0.0 1.1014905982011676 0.0 72-73 4.3597490528445184E-5 0.0 0.0 1.3425193245876768 0.0 74-75 4.3597490528445184E-5 0.0 0.0 1.6372165618147019 0.0 76-77 4.3597490528445184E-5 0.0 0.0 1.8614166568572312 0.0 78-79 4.3597490528445184E-5 0.0 0.0 2.152233717427225 0.0 80-81 4.3597490528445184E-5 0.0 0.0 2.465045711968819 0.0 82-83 4.3597490528445184E-5 0.0 0.0 2.7140527791220337 0.0 84-85 4.3597490528445184E-5 0.0 0.0 3.0784624037040427 0.0 86-87 4.3597490528445184E-5 0.0 0.0 3.565119391727812 0.0 88-89 4.3597490528445184E-5 0.0 0.0 4.225817561941135 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14095 0.0 33.946777 1 GGTATCA 9040 0.0 31.063702 1 ATCAACG 16790 0.0 28.265326 3 TCAACGC 16785 0.0 28.220842 4 CAACGCA 16935 0.0 27.944046 5 TATCAAC 17075 0.0 27.904222 2 AACGCAG 17345 0.0 27.309109 6 GTACATG 10945 0.0 25.179459 1 TAGATCG 115 1.2802414E-4 24.788462 4 TACATGG 10790 0.0 24.786583 2 TAGCCCT 1895 0.0 24.319725 4 ACGCAGA 19205 0.0 24.317888 7 CGCAGAG 19165 0.0 24.121271 8 CATGGGT 3310 0.0 23.970917 4 GCGTATC 120 1.7077872E-4 23.757685 1 ACATGGG 10700 0.0 23.219728 3 GAGTACT 9575 0.0 23.020441 12-13 GCAGAGT 20120 0.0 22.291546 9 AGTACTT 9815 0.0 21.949339 12-13 AGAGTAC 15230 0.0 21.77182 10-11 >>END_MODULE