FastQCFastQC Report
Thu 26 May 2016
SRR937436_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937436_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293710
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124040.540783272514834No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99110.43209472862742015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64570.2815089963421705No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42350.18463537238796535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA157100.030.9788861
GGTATCA107750.030.0821671
TATCAAC191600.025.2111632
ATCAACG190850.025.1857993
TCAACGC191850.025.0792794
CAACGCA194400.024.7503055
TACATGG105050.024.4606842
GTACATG106300.024.322471
AACGCAG198400.024.2507786
GAGTACT112250.022.76924112-13
ACATGGG106800.022.6812153
AGTACTT114100.022.21270212-13
CGCAGAG220600.021.6643228
AGAGTAC168450.021.6592210-11
ACGCAGA222700.021.5666947
GTACTTT116450.021.47419214-15
CATGGGT33800.020.6570384
GCAGAGT230050.020.237489
TATACTG8700.020.1999285
TACTTTT128400.019.40163814-15
Produced by FastQC (version 0.11.5)