FastQCFastQC Report
Thu 26 May 2016
SRR937435_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937435_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888651
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111201.2513348884995346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83040.9344500822032497No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55600.6256674442497673No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18450.20761806378432027No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15310.17228360740043056No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14940.16811999311315692No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39000.053.6244851
GTATCAA75100.044.3662951
ATCAACG99300.033.3474433
TATCAAC99600.033.2965772
TCAACGC100050.033.0555734
CAACGCA101100.032.6652645
AACGCAG103500.031.9042076
CGTCACT1354.1993917E-728.1663971
CGCAGAG115950.028.1507178
ACGCAGA116700.028.1326547
GTACATG65850.028.0059971
TAAGGCG856.3923205E-427.9514035
TCGCGCA700.008380203527.1497239
TACATGG67050.026.997882
GCAGAGT120200.026.76019
ACATGGG66700.026.0695923
TCGTTCG950.001223719825.009155
ATGGGAG18400.024.2751855
CATGGGA48800.023.9534344
AAGACCG1401.7738545E-523.758695
Produced by FastQC (version 0.11.5)