##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937435_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 888651 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.865157412752588 33.0 31.0 34.0 30.0 34.0 2 31.95517025243881 33.0 31.0 34.0 30.0 34.0 3 31.956740047555225 33.0 31.0 34.0 30.0 34.0 4 35.60118314163828 37.0 35.0 37.0 33.0 37.0 5 35.623609268430464 37.0 35.0 37.0 35.0 37.0 6 35.69137040300411 37.0 35.0 37.0 35.0 37.0 7 35.64193704840258 37.0 35.0 37.0 33.0 37.0 8 35.63868717865619 37.0 35.0 37.0 33.0 37.0 9 37.24142548649582 39.0 37.0 39.0 34.0 39.0 10-11 37.14353835195144 39.0 37.0 39.0 34.0 39.0 12-13 37.010992504368986 39.0 37.0 39.0 33.0 39.0 14-15 38.080685781032145 40.0 38.0 41.0 33.0 41.0 16-17 38.04042475617537 40.0 38.0 41.0 33.0 41.0 18-19 38.081721058098175 40.0 38.0 41.0 33.5 41.0 20-21 38.02720978201791 40.0 38.0 41.0 33.0 41.0 22-23 38.063006737178036 40.0 38.0 41.0 33.5 41.0 24-25 38.02411182792795 40.0 38.0 41.0 33.0 41.0 26-27 37.92576331990849 40.0 38.0 41.0 33.0 41.0 28-29 37.7763621489201 40.0 37.5 41.0 33.0 41.0 30-31 37.43449115569554 39.5 37.0 41.0 33.0 41.0 32-33 37.08406562306237 39.0 35.5 41.0 32.0 41.0 34-35 37.00813986593162 39.0 35.0 41.0 32.0 41.0 36-37 36.82012060977819 39.0 35.0 41.0 31.0 41.0 38-39 36.6491389758184 39.0 35.0 41.0 31.0 41.0 40-41 36.34817605561689 39.0 35.0 41.0 30.5 41.0 42-43 35.95627529817668 39.0 35.0 41.0 30.0 41.0 44-45 35.50610250818375 39.0 35.0 41.0 28.0 41.0 46-47 34.822330701253925 39.0 35.0 40.0 23.5 41.0 48-49 34.27593284652805 38.5 34.5 40.0 19.0 41.0 50-51 33.25614386300134 37.5 33.5 39.5 14.0 40.5 52-53 33.32234758077131 38.0 33.5 39.5 11.5 40.5 54-55 33.605844701688284 38.0 34.0 40.0 8.5 41.0 56-57 33.37146472574723 38.0 33.5 40.0 7.0 41.0 58-59 33.11089505328864 38.0 33.0 40.0 3.5 41.0 60-61 32.803718782739224 38.0 33.0 40.0 2.0 41.0 62-63 32.44101452651266 37.0 32.5 40.0 2.0 41.0 64-65 32.035317576866504 36.5 32.0 39.5 2.0 41.0 66-67 31.602569512665827 36.0 31.0 39.0 2.0 41.0 68-69 31.120662104695768 35.5 31.0 39.0 2.0 40.0 70-71 30.67028788579544 35.0 30.0 38.5 2.0 40.0 72-73 30.20073853515047 35.0 29.5 38.0 2.0 40.0 74-75 29.674682749470826 35.0 29.0 37.0 2.0 39.0 76-77 29.175582427747226 34.0 29.0 36.5 2.0 39.0 78-79 28.655323630986743 34.0 28.0 36.0 2.0 38.5 80-81 28.10781791727011 34.0 26.5 35.5 2.0 37.5 82-83 27.571301332018983 34.0 26.0 35.0 2.0 37.0 84-85 27.0273436928558 33.0 25.5 35.0 2.0 36.5 86-87 26.48417207655199 33.0 24.5 35.0 2.0 36.0 88-89 25.96105332689661 32.5 23.0 35.0 2.0 35.5 90-91 25.423098044114056 32.0 20.0 35.0 2.0 35.0 92-93 24.809937759592913 32.0 17.5 34.5 2.0 35.0 94-95 24.094917464786512 31.0 4.5 34.0 2.0 35.0 96-97 23.307847512690586 31.0 2.0 34.0 2.0 35.0 98-99 22.196327917258856 31.0 2.0 34.0 2.0 35.0 100-101 19.20558351928935 25.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.241886871721956 1101 2 0.375696089005789 1101 3 0.31369989834339407 1101 4 0.20080143603026102 1101 5 0.2857950658937014 1101 6 0.21058450662561512 1101 7 0.33895219087466444 1101 8 0.22121970945084257 1101 9 0.29730714058682395 1101 10-11 0.32968068886046353 1101 12-13 0.3916823133421232 1101 14-15 0.5653763024437666 1101 16-17 0.5794142865914012 1101 18-19 0.5262352643730495 1101 20-21 0.5084581697600825 1101 22-23 0.641655514562423 1101 24-25 0.6887818271403034 1101 26-27 0.6468797927259544 1101 28-29 0.6707012744400558 1101 30-31 0.7504720330528158 1101 32-33 0.586303305849917 1101 34-35 0.9290393368368299 1101 36-37 0.9653599364779168 1101 38-39 0.9150223114282099 1101 40-41 1.1364176245473487 1101 42-43 1.2011307437835157 1101 44-45 1.2939335428634564 1101 46-47 1.6867664579267938 1101 48-49 1.6289375712368397 1101 50-51 1.5895799312383616 1101 52-53 1.5451438688312038 1101 54-55 1.7966100554004925 1101 56-57 1.8004538713433007 1101 58-59 1.8131445280491079 1101 60-61 1.9145139004054172 1101 62-63 1.9197519284880684 1101 64-65 1.9173812313475054 1101 66-67 1.9946451953359912 1101 68-69 1.988679125488897 1101 70-71 2.0819350652790014 1101 72-73 2.1413117879585073 1101 74-75 2.115935312855541 1101 76-77 2.1264400733885154 1101 78-79 2.249453799904778 1101 80-81 2.4064885639757954 1101 82-83 2.0844963692305924 1101 84-85 2.361953165914631 1101 86-87 2.3634374331952124 1101 88-89 2.2309652360573473 1101 90-91 2.178670597021579 1101 92-93 2.3444593543775554 1101 94-95 2.571421553313172 1101 96-97 2.6261256473766146 1101 98-99 2.754128226399555 1101 100-101 2.5822417240947537 1102 1 0.29537417769576635 1102 2 0.34765882048128915 1102 3 0.2570532907347314 1102 4 0.3385161239095993 1102 5 0.33404721600089005 1102 6 0.27257538965461947 1102 7 0.20543440890715203 1102 8 0.2900328244589758 1102 9 0.44425502147218054 1102 10-11 0.26855469128820886 1102 12-13 0.39206907436383887 1102 14-15 0.5393700135416708 1102 16-17 0.5275579628227121 1102 18-19 0.5757716676892457 1102 20-21 0.45951385301636094 1102 22-23 0.4372823386023015 1102 24-25 0.5324361054016222 1102 26-27 0.48861188807965306 1102 28-29 0.4687267916280078 1102 30-31 0.6132829269292088 1102 32-33 0.6671221544772052 1102 34-35 0.9580677208343502 1102 36-37 0.8999124171721533 1102 38-39 0.8738442076548765 1102 40-41 0.8238091506714795 1102 42-43 1.0256871631125293 1102 44-45 1.2105452193013875 1102 46-47 1.4139301243437927 1102 48-49 1.227305297676054 1102 50-51 1.128574029742964 1102 52-53 1.3891335767265502 1102 54-55 1.5082487211718245 1102 56-57 1.4946269517431077 1102 58-59 1.639085603490102 1102 60-61 1.5359646093596808 1102 62-63 1.4883296939262252 1102 64-65 1.4229001626215592 1102 66-67 1.470933322611998 1102 68-69 1.6623503205094288 1102 70-71 1.643763560333106 1102 72-73 1.5862490280731798 1102 74-75 1.5034430609895324 1102 76-77 1.6728827113698692 1102 78-79 1.5694010480258562 1102 80-81 1.6835586071070736 1102 82-83 1.7562734683292263 1102 84-85 1.675297066841825 1102 86-87 1.9013978014898463 1102 88-89 1.8009441223922202 1102 90-91 1.9154587372111536 1102 92-93 2.043147337021736 1102 94-95 1.7723029035546567 1102 96-97 1.7620443823646035 1102 98-99 1.7925651049346385 1102 100-101 1.7835114495525382 1103 1 0.37252471533017584 1103 2 0.36872560033414814 1103 3 0.38325589401713245 1103 4 0.3297017492633074 1103 5 0.24620058329007577 1103 6 0.2889166460213417 1103 7 0.18824425610579 1103 8 0.18416129078608634 1103 9 0.34647630047614086 1103 10-11 0.3865725181133328 1103 12-13 0.27684553163604875 1103 14-15 0.4404240599480147 1103 16-17 0.5823966725001313 1103 18-19 0.5132080004566504 1103 20-21 0.29107865211087613 1103 22-23 0.1487141439220565 1103 24-25 0.18720407370948777 1103 26-27 0.24639343873047181 1103 28-29 0.1285598419958589 1103 30-31 0.19667285335818718 1103 32-33 0.5166128166164228 1103 34-35 0.7449239743714173 1103 36-37 0.6830123039859544 1103 38-39 0.7979528663985107 1103 40-41 1.008819054463217 1103 42-43 1.1951410397395819 1103 44-45 1.3571142628780635 1103 46-47 1.274626219420199 1103 48-49 1.5895011097869727 1103 50-51 1.5299251899014266 1103 52-53 1.54852095646622 1103 54-55 1.6987623035147763 1103 56-57 1.9108762443294083 1103 58-59 1.8095086368800395 1103 60-61 1.6432934152453527 1103 62-63 1.8918738365408245 1103 64-65 1.5480770160454398 1103 66-67 1.78252075894477 1103 68-69 2.109478220905583 1103 70-71 2.0886348110405244 1103 72-73 2.0705727405802534 1103 74-75 2.0986397219968893 1103 76-77 1.8696356825073934 1103 78-79 1.7806984447434537 1103 80-81 1.5632416857714801 1103 82-83 1.6560258735357891 1103 84-85 1.8920274007410889 1103 86-87 1.8029328892091456 1103 88-89 1.9843128264103278 1103 90-91 1.7127139720724998 1103 92-93 1.91411507895722 1103 94-95 1.6736752861239843 1103 96-97 1.6653338560488145 1103 98-99 1.711743090220434 1103 100-101 1.6323017721331858 1104 1 -0.10669460508543693 1104 2 0.05083367169198638 1104 3 -0.07626464279245937 1104 4 -0.03457203545121956 1104 5 0.03554937650309853 1104 6 0.001828453763103255 1104 7 -0.05015640012619116 1104 8 -0.02268628307696474 1104 9 0.05976647621033493 1104 10-11 0.037359553097594755 1104 12-13 0.1259081101548034 1104 14-15 0.33642717852794846 1104 16-17 0.07045480223897016 1104 18-19 0.13316765105745532 1104 20-21 0.12613681140971522 1104 22-23 0.1719176349579854 1104 24-25 -0.01737366169895438 1104 26-27 0.142888731300566 1104 28-29 0.1429189812086733 1104 30-31 0.1895248760011441 1104 32-33 0.4974568284995442 1104 34-35 0.6453832628622322 1104 36-37 0.7197261927516792 1104 38-39 0.5972489689125879 1104 40-41 0.5504081261314298 1104 42-43 0.4933611582751851 1104 44-45 0.5200035311202811 1104 46-47 0.7919188190743469 1104 48-49 0.9659225762585422 1104 50-51 1.0232002900359305 1104 52-53 0.8636338756639645 1104 54-55 0.9358294342734297 1104 56-57 0.9608045613308391 1104 58-59 0.9448350531735059 1104 60-61 1.0333597774440264 1104 62-63 0.9348936121804314 1104 64-65 1.1381334017443123 1104 66-67 1.3612586208700108 1104 68-69 1.2607788608795687 1104 70-71 1.1598976881152616 1104 72-73 1.2397649396964106 1104 74-75 1.2712845290439923 1104 76-77 1.3290058328199876 1104 78-79 1.4426621155041808 1104 80-81 1.448671931562874 1104 82-83 1.8414675738269572 1104 84-85 1.671639499648851 1104 86-87 1.4424782564182372 1104 88-89 1.4941382007138237 1104 90-91 1.596041393405951 1104 92-93 1.6508463342225959 1104 94-95 1.6680478012365327 1104 96-97 1.6821897067117 1104 98-99 1.735924278236812 1104 100-101 1.8626513930261908 1105 1 0.20326737508838377 1105 2 0.08950098439014198 1105 3 0.09761822471433845 1105 4 0.2218842781243211 1105 5 0.24883216223744853 1105 6 0.18688955757483683 1105 7 0.234735484175971 1105 8 0.35688195254410715 1105 9 0.22357838210508163 1105 10-11 0.3179787539100829 1105 12-13 0.2972323146557798 1105 14-15 0.5123107066164891 1105 16-17 0.36093725971947777 1105 18-19 0.39868659331184375 1105 20-21 0.4195715163959406 1105 22-23 0.49130345398477715 1105 24-25 0.5151538055520533 1105 26-27 0.5596172365670355 1105 28-29 0.5462241452410197 1105 30-31 0.5258588364506309 1105 32-33 0.6282913003906287 1105 34-35 0.8229237016689339 1105 36-37 0.896217921657076 1105 38-39 0.8659398675802166 1105 40-41 1.0127618778429053 1105 42-43 0.8909164237955807 1105 44-45 1.052853286603188 1105 46-47 1.1909065575712745 1105 48-49 1.494732452392192 1105 50-51 1.4132312529866056 1105 52-53 1.2962279831001666 1105 54-55 1.6196581311668083 1105 56-57 1.833124221786008 1105 58-59 1.7505549054010743 1105 60-61 1.8226407473060817 1105 62-63 1.8024342401404994 1105 64-65 1.7220498366981118 1105 66-67 1.716133612321734 1105 68-69 1.6177865565114296 1105 70-71 1.811903150282408 1105 72-73 1.949526926563344 1105 74-75 1.6761804001170617 1105 76-77 1.911470515704373 1105 78-79 1.6934518307992654 1105 80-81 1.619411579417509 1105 82-83 1.704130591288724 1105 84-85 1.7485608976959135 1105 86-87 1.5960653317143745 1105 88-89 1.7696255252558863 1105 90-91 1.8108595951976696 1105 92-93 2.019900916608343 1105 94-95 1.9754660324197744 1105 96-97 1.9759692528309287 1105 98-99 1.9651450536783024 1105 100-101 1.2315677479535658 1106 1 -0.11085018076292386 1106 2 -0.8762431182203443 1106 3 -0.2607087557619323 1106 4 -0.04476559115757084 1106 5 0.06387788127319283 1106 6 -0.06271742669161284 1106 7 0.10090339772542478 1106 8 0.03664411880375695 1106 9 0.024693039330756505 1106 10-11 -0.26514030509180486 1106 12-13 -0.30845984527255155 1106 14-15 -0.31551394591452464 1106 16-17 -0.2904836267517368 1106 18-19 -0.25292272536038496 1106 20-21 -0.17419019331941143 1106 22-23 -0.2043494689755292 1106 24-25 -0.338414992659672 1106 26-27 -0.06138207861651068 1106 28-29 0.0485876234194933 1106 30-31 -0.1358784438374201 1106 32-33 0.11231763352153479 1106 34-35 0.20866702901550838 1106 36-37 0.0393068745770222 1106 38-39 0.21071372740389194 1106 40-41 0.25963390330601754 1106 42-43 0.11532194712025756 1106 44-45 0.5406805664174641 1106 46-47 0.425236535734669 1106 48-49 0.4310660570678948 1106 50-51 0.3263004797469904 1106 52-53 0.3450351534299543 1106 54-55 0.6386133338337174 1106 56-57 0.6377017244886574 1106 58-59 0.2256021749646493 1106 60-61 0.19391248161451813 1106 62-63 0.2906696102644091 1106 64-65 0.4847867767314469 1106 66-67 0.6870784436802992 1106 68-69 0.6247930041229743 1106 70-71 0.6629544942885381 1106 72-73 0.6899836431483948 1106 74-75 0.5878519983236181 1106 76-77 0.7447866209507552 1106 78-79 0.8776556132257589 1106 80-81 0.993962034023653 1106 82-83 1.0061658744107973 1106 84-85 0.8477882179962002 1106 86-87 0.7923890733102965 1106 88-89 1.2516630910994841 1106 90-91 1.0361006807394268 1106 92-93 1.3368083457901392 1106 94-95 1.483864724807976 1106 96-97 1.1495443823646063 1106 98-99 0.9783999130755774 1106 100-101 0.972139677370091 1107 1 0.1526429710581887 1107 2 0.14080129956004228 1107 3 0.12943008781648047 1107 4 0.1485565358972991 1107 5 -0.06343686954377858 1107 6 -0.0018524052098385368 1107 7 0.12424028831492251 1107 8 0.01894170079113877 1107 9 0.13159319470728548 1107 10-11 -0.017546982411815293 1107 12-13 0.130462250817601 1107 14-15 0.2701698490326976 1107 16-17 0.18821707161321655 1107 18-19 0.2805495805710123 1107 20-21 0.17379931185335096 1107 22-23 0.35344534554028684 1107 24-25 0.09744933764537933 1107 26-27 0.20428136808823893 1107 28-29 0.2579049620020797 1107 30-31 0.14499918470762196 1107 32-33 0.16449179899476718 1107 34-35 0.27066696266254553 1107 36-37 0.5064096276721131 1107 38-39 0.5309234580661482 1107 40-41 0.7452283982217267 1107 42-43 0.5774717278790078 1107 44-45 0.6819197633254177 1107 46-47 0.8722163699628567 1107 48-49 0.9130180506980068 1107 50-51 0.8420288970629031 1107 52-53 0.8352881794016156 1107 54-55 0.8678572970962932 1107 56-57 1.2620568566140022 1107 58-59 1.032216459652048 1107 60-61 1.0867621202673377 1107 62-63 1.184489661024429 1107 64-65 0.7903407963387536 1107 66-67 0.8391580561789844 1107 68-69 1.1209459145298872 1107 70-71 1.3602135639093866 1107 72-73 1.2481848695723556 1107 74-75 1.1000858261708473 1107 76-77 1.1710740066992429 1107 78-79 0.9478280203454297 1107 80-81 1.0090628628827716 1107 82-83 1.039750150969951 1107 84-85 1.0281411051795715 1107 86-87 0.8169465819776534 1107 88-89 0.8141772602221309 1107 90-91 0.7008067094592008 1107 92-93 0.8536937929261761 1107 94-95 1.0828439950359545 1107 96-97 1.1539789721650493 1107 98-99 1.3044017046056204 1107 100-101 1.5087430138183286 1108 1 0.23811611317963965 1108 2 0.38152978822322936 1108 3 0.3239887746056951 1108 4 0.20101612390959644 1108 5 0.1574343127750808 1108 6 0.21531732513849278 1108 7 0.3711602153587634 1108 8 0.18116185671703988 1108 9 0.19586792469798553 1108 10-11 0.22157888483659605 1108 12-13 0.22190778404126377 1108 14-15 0.4284829167674786 1108 16-17 0.5977192531452928 1108 18-19 0.47798940962473324 1108 20-21 0.41374772398410187 1108 22-23 0.30522588698939757 1108 24-25 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62-63 -1.2075280773347714 2316 64-65 -1.5268432391379676 2316 66-67 -1.5638907243088198 2316 68-69 -1.2960441076430662 2316 70-71 -1.2909505159132273 2316 72-73 -1.4966322035983737 2316 74-75 -1.547253419948884 2316 76-77 -1.3501011595978731 2316 78-79 -1.4225710927366073 2316 80-81 -1.387555762394392 2316 82-83 -1.3136312237528855 2316 84-85 -0.8131560123370605 2316 86-87 -0.44632947123742284 2316 88-89 -0.4858227397778698 2316 90-91 -0.48802366748093107 2316 92-93 -0.4188394664973245 2316 94-95 -0.23447862137203046 2316 96-97 0.10522620054642218 2316 98-99 0.5571178908583931 2316 100-101 1.231165408496821 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3807.0 3 552.0 4 528.0 5 558.0 6 711.0 7 803.0 8 1021.0 9 1246.0 10 1750.0 11 2608.0 12 3980.0 13 5914.0 14 6776.0 15 9653.0 16 13117.0 17 18853.0 18 14754.0 19 7891.0 20 7095.0 21 7409.0 22 7577.0 23 8220.0 24 9173.0 25 10212.0 26 11584.0 27 13393.0 28 15298.0 29 18585.0 30 22392.0 31 27878.0 32 35291.0 33 45557.0 34 61472.0 35 86193.0 36 119424.0 37 157564.0 38 117507.0 39 12304.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.2202414888993 19.935309425292548 15.578800006773882 20.26564907903427 2 14.819734216869646 21.26061913746874 41.49825760379897 22.42138904186265 3 17.646733561733495 28.286073458186067 31.65531436618955 22.41187861389088 4 11.155547982228763 17.647218137766586 39.71501470968266 31.48221917032199 5 11.4352842401755 38.78826657277758 34.688405600845 15.088043586201916 6 27.348295645543004 38.684187371826546 18.809715576191152 15.157801406439301 7 25.251860968007932 35.90520484466458 22.024679047175354 16.81825514015213 8 26.175524012467328 33.535218623833444 20.729471830628434 19.559785533070794 9 27.337578381842263 15.801937348679889 21.7332343524007 35.127249917077144 10-11 23.365746034629336 27.99337041134251 27.79722439566775 20.843659158360406 12-13 25.304643647454405 26.26475835806339 28.831927184485185 19.598670809997014 14-15 22.407080384724065 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34.50926922131323 19.86115904174815 40-41 19.140826097522883 26.489635297109455 34.69839653425401 19.671142071113653 42-43 19.235647381335465 26.531499599878867 34.04379507707196 20.189057941713713 44-45 19.24554221718689 26.803880113712303 33.63287799161651 20.3176996774843 46-47 19.653067693310756 27.171400757800505 32.81548042724698 20.36005112164176 48-49 19.69965237334302 27.55343145245671 32.03365824740436 20.713257926795904 50-51 19.89184407765654 27.744656797316523 31.588389203362695 20.775109921664242 52-53 19.961689714901514 28.110383272115868 31.20431963980433 20.72360737317829 54-55 19.790035603247162 28.051150900911065 31.22712750835932 20.931685987482457 56-57 19.907230934750395 28.27178426512149 30.914507205221952 20.906477594906157 58-59 19.941026067493013 27.617503869107185 31.360149746429006 21.081320316970793 60-61 20.05389066588633 27.800095963500038 31.540755450382868 20.605257920230763 62-63 20.005040871089587 27.652391904609125 31.769497993519042 20.57306923078224 64-65 19.97664736964361 27.515848441316226 31.774743527002673 20.732760662037485 66-67 19.881920841518397 27.75660875664484 31.318097652400034 21.043372749436724 68-69 19.954001705060602 27.5807084571908 31.48334633930706 20.981943498441538 70-71 19.930623531163025 27.766882885483717 31.396348037115057 20.906145546238196 72-73 20.008548179649356 27.637967675927005 31.147796108999444 21.205688035424195 74-75 19.638593756872368 28.087702421216576 30.992733756398707 21.280970065512353 76-77 19.64668818819835 27.889446102549876 31.202047478213395 21.26181823103838 78-79 19.76147493693416 27.60002413787464 31.05957938061537 21.57892154457583 80-81 19.559906750442394 27.92219729702601 30.784139905915843 21.733756046615756 82-83 19.829838245402144 27.6943922403804 30.78930853250288 21.686460981714575 84-85 19.51090391329865 27.240135286214684 30.870807753868846 22.378153046617815 86-87 19.6249020392745 27.231277520621084 30.91741981315577 22.226400626948642 88-89 19.67663756295717 26.854769407046692 31.07788982693405 22.390703203062092 90-91 19.236557545192554 26.980597891023628 31.44349457869811 22.33934998508571 92-93 19.28784336546367 27.071470540802366 31.319238390393156 22.32144770334081 94-95 18.916585752579948 26.861841593336518 31.49379245170247 22.727780202381073 96-97 19.134445938820797 26.997685126443976 31.26015400535156 22.60771492938367 98-99 19.124912024254236 27.278628209986827 30.812038691281828 22.784421074477105 100-101 19.268744841024624 26.63920099593597 31.425771647398975 22.666282515640436 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1753.0 1 1075.0 2 473.5 3 588.5 4 495.0 5 423.0 6 802.0 7 1795.0 8 3125.0 9 3734.5 10 3184.5 11 2569.0 12 2525.0 13 2689.5 14 2853.5 15 2946.0 16 3046.5 17 3229.5 18 3361.0 19 3478.0 20 3707.0 21 4187.5 22 4735.0 23 5254.0 24 5918.0 25 6834.5 26 8308.0 27 9910.5 28 11025.5 29 12421.0 30 14506.5 31 16525.5 32 18699.0 33 21752.0 34 25391.5 35 27764.0 36 30261.5 37 32833.0 38 33036.5 39 32440.5 40 31402.0 41 30750.5 42 30064.5 43 30238.5 44 31379.0 45 31182.5 46 29734.0 47 28495.5 48 28933.5 49 28045.0 50 25275.0 51 22526.5 52 20267.0 53 19255.0 54 18593.5 55 17294.5 56 15663.0 57 13675.0 58 12145.0 59 11021.5 60 9709.5 61 8462.0 62 7089.5 63 5334.5 64 3724.5 65 2665.0 66 1835.0 67 1348.0 68 968.5 69 628.5 70 487.5 71 392.0 72 296.5 73 254.0 74 212.0 75 167.0 76 120.0 77 75.0 78 50.0 79 30.0 80 21.0 81 17.0 82 9.5 83 5.5 84 6.5 85 5.5 86 3.0 87 2.5 88 2.0 89 1.5 90 0.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32588721556606587 2 0.2830132414187347 3 0.2798623981743114 4 0.2813252896806508 5 0.28121275956477854 6 0.24272745993646547 7 0.24193974912535965 8 0.2446404719062939 9 0.2571313147681148 10-11 0.2612386639974523 12-13 0.2527989053070328 14-15 0.24143336360393453 16-17 0.238620110707128 18-19 0.22618553290324325 20-21 0.23046167730638914 22-23 0.22843613522068842 24-25 0.2298427616690917 26-27 0.22652312325086 28-29 0.2458783031808888 30-31 0.2629266157355362 32-33 0.2516736041483102 34-35 0.24970432712054566 36-37 0.2332186651452595 38-39 0.21977131629852437 40-41 0.2155514369533146 42-43 0.23006782190083622 44-45 0.22815480993100778 46-47 0.24058938773489252 48-49 0.2488603512515037 50-51 0.26309541090934463 52-53 0.27749926574099393 54-55 0.24925420665705658 56-57 0.2175207139810792 58-59 0.20463601571370538 60-61 0.208799630000979 62-63 0.21375095509935846 64-65 0.20401710007640797 66-67 0.2107689070287436 68-69 0.21144408772397713 70-71 0.21324456957793328 72-73 0.21521384660569787 74-75 0.2192086657191631 76-77 0.23597565298412987 78-79 0.2337813157246208 80-81 0.22483517151277613 82-83 0.21453866591046428 84-85 0.20148517246928208 86-87 0.2043546904240247 88-89 0.21797083444456822 90-91 0.21577649718505917 92-93 0.21943372595090763 94-95 0.2147074610842727 96-97 0.22432878599135095 98-99 0.22989902672702783 100-101 0.20784312401606478 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 888651.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.59412797715874 #Duplication Level Percentage of deduplicated Percentage of total 1 77.57826918875652 39.250048795824185 2 12.223125852694446 12.368367873442807 3 3.9910231850841074 6.057670133578591 4 1.8156180774064994 3.674384534637894 5 1.0098159147507964 2.5545377812136705 6 0.6330463346017973 1.921705636098875 7 0.4427377428274891 1.5679951014652667 8 0.30056234658953335 1.2165351862772793 9 0.2366464259043927 1.0775627601789652 >10 1.552529568521712 15.11572582282808 >50 0.1431525115822495 5.038438429555009 >100 0.06989970350307727 6.039982680452379 >500 0.0022332173608678083 0.7439109177539818 >1k 6.699652082603426E-4 0.5502500257384404 >5k 4.466434721735617E-4 1.5664612642377285 >10k+ 2.2332173608678084E-4 1.2564230567169317 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11120 1.2513348884995346 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8304 0.9344500822032497 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5560 0.6256674442497673 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1845 0.20761806378432027 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1531 0.17228360740043056 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1494 0.16811999311315692 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.6265057936130154E-5 0.0 18-19 0.0 0.0 0.0 1.1253011587226031E-4 0.0 20-21 0.0 0.0 0.0 2.813252896806508E-4 0.0 22-23 0.0 0.0 0.0 4.5012046348904123E-4 0.0 24-25 0.0 0.0 0.0 0.001406626448403254 0.0 26-27 0.0 0.0 0.0 0.0022506023174452064 0.0 28-29 0.0 0.0 0.0 0.004726264866634933 0.0 30-31 0.0 0.0 0.0 0.010183975486439558 0.0 32-33 0.0 0.0 0.0 0.016091806569733226 0.0 34-35 0.0 0.0 0.0 0.022168432826835283 0.0 36-37 0.0 0.0 0.0 0.027794938620448295 0.0 38-39 0.0 0.0 0.0 0.04101722723543888 0.0 40-41 0.0 0.0 0.0 0.05958469635436184 0.0 42-43 0.0 0.0 0.0 0.08653565910576819 0.0 44-45 0.0 0.0 0.0 0.11303650139368548 0.0 46-47 0.0 0.0 0.0 0.16609445102745624 0.0 48-49 0.0 0.0 0.0 0.22736709911990197 0.0 50-51 0.0 0.0 0.0 0.25904432673794325 0.0 52-53 0.0 0.0 0.0 0.310808180039183 0.0 54-55 0.0 0.0 0.0 0.3943055260164001 0.0 56-57 0.0 0.0 0.0 0.5300731108162822 0.0 58-59 0.0 0.0 0.0 0.6969552726548442 0.0 60-61 0.0 0.0 0.0 0.8985529752399986 0.0 62-63 0.0 0.0 0.0 1.1110660990647623 0.0 64-65 0.0 0.0 0.0 1.3395022342854506 0.0 66-67 0.0 0.0 0.0 1.6311802946263492 0.0 68-69 0.0 0.0 0.0 1.9814302802787598 0.0 70-71 0.0 0.0 0.0 2.2174059332628895 0.0 72-73 0.0 0.0 0.0 2.514710499397401 0.0 74-75 0.0 0.0 0.0 2.8459428954673998 0.0 76-77 0.0 0.0 0.0 3.2100903504300335 0.0 78-79 0.0 0.0 0.0 3.6473823807096375 0.0 80-81 0.0 0.0 0.0 4.275187897160977 0.0 82-83 0.0 0.0 0.0 4.895285100675068 0.0 84-85 0.0 0.0 0.0 5.7014508507839405 0.0 86-87 0.0 0.0 0.0 6.609400090699273 0.0 88-89 0.0 0.0 0.0 7.74831739344242 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3900 0.0 53.624485 1 GTATCAA 7510 0.0 44.366295 1 ATCAACG 9930 0.0 33.347443 3 TATCAAC 9960 0.0 33.296577 2 TCAACGC 10005 0.0 33.055573 4 CAACGCA 10110 0.0 32.665264 5 AACGCAG 10350 0.0 31.904207 6 CGTCACT 135 4.1993917E-7 28.166397 1 CGCAGAG 11595 0.0 28.150717 8 ACGCAGA 11670 0.0 28.132654 7 GTACATG 6585 0.0 28.005997 1 TAAGGCG 85 6.3923205E-4 27.951403 5 TCGCGCA 70 0.0083802035 27.149723 9 TACATGG 6705 0.0 26.99788 2 GCAGAGT 12020 0.0 26.7601 9 ACATGGG 6670 0.0 26.069592 3 TCGTTCG 95 0.0012237198 25.00915 5 ATGGGAG 1840 0.0 24.275185 5 CATGGGA 4880 0.0 23.953434 4 AAGACCG 140 1.7738545E-5 23.75869 5 >>END_MODULE