FastQCFastQC Report
Thu 26 May 2016
SRR937435_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937435_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888651
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86190.9698970687030116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70350.7916493651613513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47150.5305794963377073No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16490.18556216107335727No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13060.14696433132917197No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.14336336762125965No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG250.005021537756.9981779
GGTATCA51300.039.6551361
GTATCAA82550.035.122531
ATCAACG105700.027.4116123
TCAACGC106650.027.1674394
CGTATAG1405.7710713E-727.1603281
TATCAAC107100.027.097642
GTATAGG4950.026.8859791
CAACGCA107900.026.8527095
AACGCAG109500.026.5904756
GTACATG78900.024.699031
GGTACTC2900.024.5681788
ACGCAGA123700.023.4996537
TACATGG82350.023.4175872
CTGGTCG2653.092282E-1123.3011429
CGCAGAG124150.023.1849218
GAGTACT64400.023.12231812-13
ACATGGG79750.022.7516233
AGTACTT66850.022.52358812-13
CGTCTTA853.1514683E-622.35222614-15
Produced by FastQC (version 0.11.5)