##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937435_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 888651 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24728155372581 33.0 31.0 34.0 31.0 34.0 2 32.43600018454939 34.0 31.0 34.0 31.0 34.0 3 32.47600576604314 34.0 31.0 34.0 31.0 34.0 4 36.11992446978623 37.0 35.0 37.0 35.0 37.0 5 36.063090009463785 37.0 35.0 37.0 35.0 37.0 6 36.1177796457777 37.0 35.0 37.0 35.0 37.0 7 36.05638884106359 37.0 35.0 37.0 35.0 37.0 8 36.04248236934409 37.0 35.0 37.0 35.0 37.0 9 37.72139906442462 39.0 37.0 39.0 35.0 39.0 10-11 37.64924137822385 39.0 37.0 39.0 35.0 39.0 12-13 37.545850958362735 39.0 37.0 39.0 35.0 39.0 14-15 38.71434117555711 40.0 38.0 41.0 34.5 41.0 16-17 38.68707287787894 40.0 38.0 41.0 34.5 41.0 18-19 38.759633421894534 40.0 38.0 41.0 35.0 41.0 20-21 38.70448803861133 40.0 38.0 41.0 34.5 41.0 22-23 38.63217731145298 40.0 38.0 41.0 34.0 41.0 24-25 38.643008335105684 40.0 38.0 41.0 34.0 41.0 26-27 38.46666013991995 40.0 38.0 41.0 34.0 41.0 28-29 38.31229414021928 40.0 38.0 41.0 34.0 41.0 30-31 38.257969664131366 40.0 37.5 41.0 34.0 41.0 32-33 38.068920194767124 40.0 37.0 41.0 34.0 41.0 34-35 37.90745860861013 40.0 37.0 41.0 33.0 41.0 36-37 37.702811902535416 40.0 37.0 41.0 33.0 41.0 38-39 37.55674218562743 40.0 36.0 41.0 33.0 41.0 40-41 37.30846192712325 40.0 36.0 41.0 33.0 41.0 42-43 37.01893656789898 40.0 36.0 41.0 32.5 41.0 44-45 36.569222900778826 40.0 35.5 41.0 31.0 41.0 46-47 35.996779950734314 39.0 35.0 41.0 29.5 41.0 48-49 35.493378727982076 39.0 35.0 41.0 27.5 41.0 50-51 35.184375531001486 39.0 35.0 41.0 26.0 41.0 52-53 34.924083807929094 39.0 35.0 40.0 24.5 41.0 54-55 34.69175075479575 38.5 35.0 40.0 24.0 41.0 56-57 34.40288032084587 38.0 34.0 40.0 23.0 41.0 58-59 34.1395947340407 38.0 34.0 40.0 22.5 41.0 60-61 33.800640521419545 38.0 34.0 40.0 21.5 41.0 62-63 33.43865589528397 37.0 33.5 40.0 19.0 41.0 64-65 33.026462582048524 37.0 33.0 39.5 17.5 41.0 66-67 32.59480493467064 36.0 32.5 39.0 13.0 40.5 68-69 32.13904221117177 36.0 32.0 39.0 8.0 40.0 70-71 31.70505631569649 35.0 31.5 38.5 7.0 40.0 72-73 31.244660164676574 35.0 31.0 37.5 2.0 40.0 74-75 30.762022436254504 35.0 31.0 37.0 2.0 39.0 76-77 29.45814779930479 33.5 29.5 35.5 2.0 38.0 78-79 30.065095858779205 34.0 30.0 36.0 2.0 38.5 80-81 29.879229303742413 34.0 30.0 36.0 2.0 37.5 82-83 29.48929726067939 34.0 30.0 35.0 2.0 37.0 84-85 29.070039306769473 34.0 29.0 35.0 2.0 36.5 86-87 28.715745551403195 34.0 29.0 35.0 2.0 36.0 88-89 28.30819410544747 34.0 29.0 35.0 2.0 36.0 90-91 27.802043209313894 34.0 27.5 35.0 2.0 35.0 92-93 27.34443105336066 33.0 26.5 35.0 2.0 35.0 94-95 26.90994664947206 33.0 26.0 35.0 2.0 35.0 96-97 26.30363438515233 33.0 25.0 35.0 2.0 35.0 98-99 25.42247912847676 32.0 22.0 34.0 2.0 35.0 100-101 22.61857973490155 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2348221455964108 1101 2 0.3139638289338009 1101 3 0.2166062035173013 1101 4 0.07426743657023138 1101 5 0.1305793319248778 1101 6 0.16592353507483892 1101 7 0.17090677221614214 1101 8 0.12675612295696936 1101 9 0.2439537756963901 1101 10-11 0.20900438915281683 1101 12-13 0.1624576993115383 1101 14-15 0.40486751069425964 1101 16-17 0.27242372036987916 1101 18-19 0.19374646319516842 1101 20-21 0.35436284266911855 1101 22-23 0.4190796102974659 1101 24-25 0.2962772331751111 1101 26-27 0.15306171938971858 1101 28-29 0.10645701365327653 1101 30-31 0.41215101877565985 1101 32-33 0.31727875870008404 1101 34-35 0.3760339458378752 1101 36-37 0.41410789692171335 1101 38-39 0.34556239028089664 1101 40-41 0.41934068329018004 1101 42-43 0.4161573815255366 1101 44-45 0.3432149719053683 1101 46-47 0.28376957715774154 1101 48-49 0.36813261861263413 1101 50-51 0.45942258418433823 1101 52-53 0.442351156886744 1101 54-55 0.35090346615866963 1101 56-57 0.36542078627109476 1101 58-59 0.4864132404571748 1101 60-61 0.46552266252119523 1101 62-63 0.42263629456702745 1101 64-65 0.41272480146304247 1101 66-67 0.5695441029620696 1101 68-69 0.6141290781932796 1101 70-71 0.6255238490028638 1101 72-73 0.7064256875271475 1101 74-75 0.5566263314057984 1101 76-77 0.5376861716940304 1101 78-79 0.6993012216007415 1101 80-81 0.6685634671542999 1101 82-83 0.4929964010759029 1101 84-85 0.6283277155027669 1101 86-87 0.7165989817830365 1101 88-89 0.8809841369764477 1101 90-91 0.8987365564741765 1101 92-93 0.7333704544467103 1101 94-95 0.9335145478345552 1101 96-97 1.0208482678243236 1101 98-99 1.382644948411368 1101 100-101 1.8388432287261018 1102 1 0.1896660206964924 1102 2 0.19550454537792916 1102 3 0.19448528097281326 1102 4 0.15689289177310428 1102 5 0.06801444130270085 1102 6 0.12581854785926083 1102 7 0.11348857959752934 1102 8 0.13179951250731392 1102 9 0.18532558281950884 1102 10-11 0.19755232427510094 1102 12-13 0.2185167400925465 1102 14-15 0.35440391401046156 1102 16-17 0.3281024569075015 1102 18-19 0.32974849062356526 1102 20-21 0.3289915779153887 1102 22-23 0.38517958705305944 1102 24-25 0.2116595390197844 1102 26-27 0.2606226068093278 1102 28-29 0.2532822157504384 1102 30-31 0.3231585344656267 1102 32-33 0.4261594116095111 1102 34-35 0.32412499932583216 1102 36-37 0.3281720514711566 1102 38-39 0.2919691931425419 1102 40-41 0.24190873773373056 1102 42-43 0.3696747115185701 1102 44-45 0.369440145592705 1102 46-47 0.4429620924603128 1102 48-49 0.37285542911909886 1102 50-51 0.4261175530626673 1102 52-53 0.3232749281618226 1102 54-55 0.4121495598077871 1102 56-57 0.4891665570812833 1102 58-59 0.6081903395558115 1102 60-61 0.6178451656850186 1102 62-63 0.6197268702533876 1102 64-65 0.6553950667074915 1102 66-67 0.8821748527231676 1102 68-69 0.7669965334478022 1102 70-71 0.964769439023268 1102 72-73 0.988081011786555 1102 74-75 0.9671673448617248 1102 76-77 0.890164606054423 1102 78-79 1.0582461194545054 1102 80-81 0.9522187785001499 1102 82-83 0.927291901992767 1102 84-85 0.9226831998902796 1102 86-87 1.1420519247825993 1102 88-89 1.120109308081073 1102 90-91 1.4718285784788776 1102 92-93 1.336181412890884 1102 94-95 1.2660454112349555 1102 96-97 1.414515459642292 1102 98-99 1.643900081550136 1102 100-101 2.361773508433739 1103 1 0.15808707332807614 1103 2 0.17537155160317042 1103 3 0.22578126286302336 1103 4 0.18622226358974814 1103 5 0.03601104571696112 1103 6 0.09880949296395869 1103 7 0.15846594235927114 1103 8 -0.09852023848306146 1103 9 0.0058264317159384404 1103 10-11 0.1374292342920782 1103 12-13 0.1491477531089629 1103 14-15 0.16596305379540865 1103 16-17 0.13373346992391077 1103 18-19 0.10525075434739506 1103 20-21 0.2065382672192868 1103 22-23 0.09939860233319564 1103 24-25 0.20047532849346794 1103 26-27 0.030783897131904325 1103 28-29 -0.06665270218957886 1103 30-31 0.030940085116441196 1103 32-33 0.09226014731975596 1103 34-35 0.22632505591892738 1103 36-37 0.19246180812084646 1103 38-39 0.23404615975261578 1103 40-41 0.2004613693126842 1103 42-43 0.15493928424069736 1103 44-45 0.4016062463284129 1103 46-47 0.4469236091552702 1103 48-49 0.2813231710545807 1103 50-51 0.28130289545089937 1103 52-53 0.33788302097450185 1103 54-55 0.3866755926317893 1103 56-57 0.5079625389714977 1103 58-59 0.4917146745869374 1103 60-61 0.4582526359736434 1103 62-63 0.38270083742938255 1103 64-65 0.3962835783090739 1103 66-67 0.5493367089767105 1103 68-69 0.6104317343532912 1103 70-71 0.6747864169519637 1103 72-73 0.44224767845322077 1103 74-75 0.4671744189986775 1103 76-77 0.3988445720985645 1103 78-79 0.45081120151449383 1103 80-81 0.38628782207683443 1103 82-83 0.4970782630567143 1103 84-85 0.5130340770832618 1103 86-87 0.43591864804236025 1103 88-89 0.4996155333215917 1103 90-91 0.7835433492768402 1103 92-93 0.8099717354715281 1103 94-95 0.817566350680341 1103 96-97 0.8326959916173884 1103 98-99 1.0775517510464567 1103 100-101 1.585408200001364 1104 1 0.04830123552378751 1104 2 0.1699827637109408 1104 3 0.11750889702847189 1104 4 0.05912609013167014 1104 5 0.015818610120810206 1104 6 0.004707451846002186 1104 7 0.06678708219285312 1104 8 0.056526392416003546 1104 9 0.05624101070765164 1104 10-11 0.009581497849033838 1104 12-13 0.06930213020587672 1104 14-15 0.13564151490183463 1104 16-17 0.10721556395427001 1104 18-19 -0.03363948937480643 1104 20-21 0.03614297612719497 1104 22-23 -0.05627691755329778 1104 24-25 -0.06335099173000458 1104 26-27 0.02334918778060313 1104 28-29 -0.11001574332231456 1104 30-31 -0.14731188431851194 1104 32-33 -0.0970014438950102 1104 34-35 -0.1802479794496179 1104 36-37 0.032918362311711746 1104 38-39 -0.09422465418196424 1104 40-41 -0.10237306513062805 1104 42-43 -0.00746778651115676 1104 44-45 -0.15863924946667396 1104 46-47 -0.36106608918493777 1104 48-49 -0.16874001344415035 1104 50-51 -0.2407544066079481 1104 52-53 -0.14808041330623212 1104 54-55 -0.005307366028560523 1104 56-57 -0.0958658504519363 1104 58-59 -0.2081741059152904 1104 60-61 -0.09555062745029375 1104 62-63 -0.1051814103129658 1104 64-65 -0.11250313151519009 1104 66-67 -0.17424958405156588 1104 68-69 0.002732463007284025 1104 70-71 0.061890128209885376 1104 72-73 0.27042094438975894 1104 74-75 0.3378575505850172 1104 76-77 0.20632251294900428 1104 78-79 0.2658727602132629 1104 80-81 0.24035232199554457 1104 82-83 0.0636355577255685 1104 84-85 0.18557283763248478 1104 86-87 0.30897212208128977 1104 88-89 0.37007363941242843 1104 90-91 0.4512793489221174 1104 92-93 0.7126465459180338 1104 94-95 0.555341073924847 1104 96-97 0.7557199056569139 1104 98-99 0.6357849498817956 1104 100-101 1.3629825064498782 1105 1 0.096229060420157 1105 2 0.14365625299394935 1105 3 0.09339329813254693 1105 4 0.08265895116360156 1105 5 0.09116057757770335 1105 6 0.06484439888160409 1105 7 -0.05602364949601224 1105 8 0.07466401749366725 1105 9 0.26980243389756 1105 10-11 0.2338795569900114 1105 12-13 0.2106444352287653 1105 14-15 0.2168697895466991 1105 16-17 0.20792445256185488 1105 18-19 0.21043891905969048 1105 20-21 0.23035655646572195 1105 22-23 0.17178747607194822 1105 24-25 0.23292465128231044 1105 26-27 0.058806349636228106 1105 28-29 0.019693993565354617 1105 30-31 0.2110464390842708 1105 32-33 0.3668942714902812 1105 34-35 0.18438114172930398 1105 36-37 0.11354170994795254 1105 38-39 0.03535740418494271 1105 40-41 0.06658490353684243 1105 42-43 0.20284856521514882 1105 44-45 0.15973278027988158 1105 46-47 0.3728871579235644 1105 48-49 0.6188447600010392 1105 50-51 0.4702516273843642 1105 52-53 0.6666304075757239 1105 54-55 0.5507220429544759 1105 56-57 0.5615417590423917 1105 58-59 0.675132091185425 1105 60-61 0.6256715069853698 1105 62-63 0.6335075822707665 1105 64-65 0.5206131847085018 1105 66-67 0.6909942855606559 1105 68-69 0.8202376610872264 1105 70-71 0.8775361669746822 1105 72-73 0.8552442242217886 1105 74-75 0.7698241781114881 1105 76-77 0.7255739603359963 1105 78-79 0.7571924395837613 1105 80-81 0.8639353086689603 1105 82-83 0.7409242770554414 1105 84-85 0.9431495211338046 1105 86-87 1.1564963227991996 1105 88-89 1.264584627040346 1105 90-91 1.352028032487457 1105 92-93 1.1520701810673835 1105 94-95 1.2855374442172902 1105 96-97 1.4165406421037083 1105 98-99 1.6302969560278235 1105 100-101 2.2189960556508517 1106 1 -0.10185517631098406 1106 2 0.0552201756570696 1106 3 0.04534201011769312 1106 4 0.10954553436018699 1106 5 -0.019665552261770358 1106 6 -0.008971493167202027 1106 7 -0.11279450301850602 1106 8 -0.1347498399180722 1106 9 -0.1147930721559618 1106 10-11 -0.24543597111544813 1106 12-13 -0.24819551153644426 1106 14-15 -0.15109845908905584 1106 16-17 -0.13044271242722516 1106 18-19 -0.1810521287956277 1106 20-21 -0.10702322379002993 1106 22-23 0.09328231410639631 1106 24-25 0.0406807360272623 1106 26-27 0.030846241271561325 1106 28-29 0.11265998101469421 1106 30-31 0.009511201185922857 1106 32-33 0.1528105257596195 1106 34-35 -0.2998583889776043 1106 36-37 0.2549196984826594 1106 38-39 0.3751221977278547 1106 40-41 0.2157553711501876 1106 42-43 0.1985189316134921 1106 44-45 0.24403088648242033 1106 46-47 0.23614244802301698 1106 48-49 0.33325583938376013 1106 50-51 0.260570734624892 1106 52-53 0.085109253638457 1106 54-55 0.17502161354441625 1106 56-57 0.2634958548047237 1106 58-59 0.21855656109872967 1106 60-61 0.16008030364656634 1106 62-63 0.28587601352423775 1106 64-65 0.31744558557162605 1106 66-67 0.2815795667445684 1106 68-69 0.4700272630753517 1106 70-71 0.5989300053493913 1106 72-73 0.8182867474474058 1106 74-75 0.7941814627927037 1106 76-77 0.5129212225776314 1106 78-79 0.7475802438210053 1106 80-81 0.5649289385838117 1106 82-83 0.6118396053151045 1106 84-85 0.8931320776957641 1106 86-87 1.0466844965789157 1106 88-89 0.9895737518104575 1106 90-91 1.0857614881402071 1106 92-93 1.1302335808580644 1106 94-95 0.4729585718817262 1106 96-97 0.8963471706872603 1106 98-99 1.194572794927982 1106 100-101 1.8787844086026695 1107 1 0.022481985219776845 1107 2 0.04078564115076233 1107 3 0.05336518941999202 1107 4 -0.017617157579586262 1107 5 0.03937199798677682 1107 6 -0.005346962476195927 1107 7 0.020635779368646467 1107 8 -0.09348659006045068 1107 9 -0.0996701256419783 1107 10-11 -0.02887371821112339 1107 12-13 0.03534314735956201 1107 14-15 -0.15013081168870457 1107 16-17 -0.044594417421947696 1107 18-19 -0.06851611848880168 1107 20-21 -0.011459748885272347 1107 22-23 -0.19110787450190259 1107 24-25 -0.3314391306032789 1107 26-27 -0.32503650198116674 1107 28-29 -0.2467767929041642 1107 30-31 -0.17231389239940142 1107 32-33 -0.016834651762209774 1107 34-35 -0.11594455936095471 1107 36-37 -0.09149392378096621 1107 38-39 0.010063392412980932 1107 40-41 0.09032891511732544 1107 42-43 0.13744793947078904 1107 44-45 0.28158251738299356 1107 46-47 0.3139316225368418 1107 48-49 0.3047710646244326 1107 50-51 0.3272485522758899 1107 52-53 0.42952537519708756 1107 54-55 0.26122240867410085 1107 56-57 0.24147772938655265 1107 58-59 0.3485406363872201 1107 60-61 0.4524439068337216 1107 62-63 0.3613690989923839 1107 64-65 0.4972482960902269 1107 66-67 0.4345673854461509 1107 68-69 0.45969555354651703 1107 70-71 0.12572001480468842 1107 72-73 0.3429313443807942 1107 74-75 0.15315100125254233 1107 76-77 0.16461654082303667 1107 78-79 0.2071616119085533 1107 80-81 0.08143700323371661 1107 82-83 0.22657217522071704 1107 84-85 0.30383619323839284 1107 86-87 0.32387815323549773 1107 88-89 0.45566738690233066 1107 90-91 0.46353518029562935 1107 92-93 0.6296502277109965 1107 94-95 0.6238175663025451 1107 96-97 0.6697296789399125 1107 98-99 0.5366679450792127 1107 100-101 1.1208635234541227 1108 1 0.2191015045674618 1108 2 0.15518196473276902 1108 3 -0.09785342870460312 1108 4 0.08390902080536478 1108 5 -0.043275881277942574 1108 6 0.1576733865689377 1108 7 -0.05949529137021159 1108 8 0.06607370605570395 1108 9 0.15830945378724692 1108 10-11 0.11670555008155503 1108 12-13 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-0.5873469021968738 2316 88-89 -0.27626165966086447 2316 90-91 -0.2364925358906227 2316 92-93 -0.38321008271028845 2316 94-95 -0.2842478438970133 2316 96-97 0.10271194058070776 2316 98-99 -0.019956223435201537 2316 100-101 0.6391562063809495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 3.0 5 22.0 6 49.0 7 131.0 8 247.0 9 479.0 10 918.0 11 1643.0 12 2540.0 13 3835.0 14 4862.0 15 7369.0 16 10641.0 17 15943.0 18 13494.0 19 6876.0 20 5531.0 21 5350.0 22 5367.0 23 5741.0 24 6523.0 25 7397.0 26 8645.0 27 10234.0 28 12543.0 29 15382.0 30 19108.0 31 24218.0 32 30940.0 33 41345.0 34 56619.0 35 83125.0 36 127280.0 37 190965.0 38 150596.0 39 12684.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.715980488068475 21.097360395462225 15.463869941470959 19.72278917499834 2 15.488870208889654 22.51727618603929 40.03821522735022 21.955638377720838 3 18.55947948069602 28.668622440080526 31.08621944948017 21.685678629743286 4 12.153927694899346 18.371329126957605 38.47415914684167 31.000584031301376 5 11.976278357940224 40.18455167446885 33.59054285199856 14.248627115592367 6 28.511530398322847 38.85136009524549 17.859429629854688 14.777679876576968 7 26.52042252807908 36.14444815793827 21.019612873895376 16.315516440087276 8 27.26278370248838 34.27273474063497 19.877769788139553 18.586711768737107 9 28.314152575082908 16.342748728128363 20.949731671938704 34.39336702485002 10-11 24.231278645947622 28.720780148787316 26.83500046699998 20.21294073826508 12-13 25.948252044940585 26.766790963942537 27.93931547031242 19.34564152080446 14-15 22.949166770757024 27.0835794929618 27.895315483806353 22.07193825247482 16-17 20.054554600174875 30.709637416713647 28.252542336642843 20.983265646468634 18-19 20.026590866380616 27.803265849022846 32.92254214534165 19.247601139254893 20-21 21.419211307958097 26.725021197920213 33.153547212424265 18.702220281697432 22-23 20.9398487356565 27.098452359383913 32.83782126230669 19.123877642652896 24-25 20.43133991523092 27.332767305895068 33.09769487295047 19.13819790592354 26-27 20.34117648382737 27.508493401648153 33.16179606608367 18.9885340484408 28-29 19.840279356390962 27.367957290792177 33.44101038246078 19.350752970356083 30-31 20.026422145538984 27.281990573325675 33.40432128113647 19.287265999998876 32-33 19.916311117387806 27.70487993846836 32.924604047607126 19.454204896536705 34-35 20.217498207957902 27.43332309309279 33.044130935541624 19.305047763407682 36-37 20.521172271888666 27.3322301624767 32.607870023141835 19.538727542492804 38-39 20.16333764699263 27.792606763067575 32.60141788105553 19.442637708884263 40-41 20.073797249989028 27.855873678193127 32.78401757270289 19.28631149911495 42-43 20.224014135230036 28.0715989916269 32.01714009183398 19.687246781309085 44-45 20.238653869741892 28.26199486637611 31.707160628863303 19.7921906350187 46-47 20.63340951622178 28.63295039334902 30.862003193604686 19.871636896824512 48-49 20.641455419506645 29.020504112413086 30.249164182564357 20.088876285515912 50-51 20.83270035143155 29.147156757827315 29.865942872961377 20.154200017779758 52-53 20.949619141822833 29.47591349134812 29.504833731127295 20.069633635701756 54-55 20.602913854820397 29.580228908761708 29.411546265069187 20.405310971348708 56-57 20.674089153109602 29.861666728558227 29.190987237959558 20.27325688037261 58-59 20.752803969162247 29.043910376514514 29.6914086632435 20.51187699107974 60-61 20.6412303592749 29.473662889030678 29.768491792615997 20.116614959078426 62-63 20.65608433457004 29.258674102656723 30.013638650043717 20.07160291272952 64-65 20.4333309702009 29.126507481564758 30.01448262591276 20.425678922321584 66-67 20.46737133025226 29.442998432455486 29.503989755258253 20.585640482034005 68-69 20.51739096675748 29.33474446098637 29.63064240067248 20.517222171583672 70-71 20.502762051694084 29.610161919583728 29.470174455438634 20.41690157328355 72-73 20.577955325493726 29.50891802171834 29.158948967535025 20.754177685252913 74-75 20.427760729465223 29.988094313740714 28.805290265807386 20.778854690986677 76-77 20.460844583531667 29.541799874191334 29.295696510778697 20.701659031498306 78-79 20.57258698859282 29.4198172285858 28.832016168327044 21.175579614494332 80-81 20.25052579696641 29.918606967189593 28.73147050979518 21.09939672604881 82-83 20.46736244389642 29.685876217371028 28.77983540136601 21.066925937366545 84-85 20.132290627519627 29.360788477346805 28.725611166157183 21.781309728976385 86-87 19.981916410379327 29.30666819707624 28.98466327050777 21.72675212203666 88-89 20.0765542378279 28.959287729378573 29.061915195054077 21.90224283773945 90-91 19.84820195622301 29.20457960112498 29.116627220333374 21.830591222318635 92-93 19.65533713728842 29.392220553623442 28.945975097804332 22.006467211283802 94-95 19.344995954542334 29.185304467108008 29.104957964375217 22.36474161397444 96-97 19.663006061997343 29.50050132166621 28.64037738099659 22.196115235339857 98-99 19.315353210720104 29.854340526282623 28.364661823354133 22.465644439643143 100-101 19.162513674486448 29.732722877247003 28.46718573441559 22.63757771385096 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 738.0 1 511.5 2 323.0 3 408.5 4 420.5 5 453.0 6 744.0 7 1392.5 8 2100.0 9 2476.5 10 2466.5 11 2375.0 12 2457.0 13 2566.5 14 2774.5 15 2982.0 16 3008.0 17 3108.0 18 3280.0 19 3411.5 20 3579.0 21 3959.0 22 4506.5 23 5005.0 24 5507.0 25 6357.0 26 7770.5 27 9481.0 28 10660.0 29 12296.0 30 14511.5 31 16095.0 32 18515.5 33 21831.0 34 25492.0 35 27674.5 36 30456.0 37 33144.5 38 33367.0 39 33174.0 40 31728.0 41 30676.0 42 30114.5 43 30433.5 44 31620.5 45 31316.0 46 29478.5 47 28128.0 48 29065.0 49 28541.5 50 25776.5 51 22993.5 52 20575.5 53 19413.0 54 18630.5 55 17430.5 56 15806.5 57 14084.0 58 13048.0 59 12176.5 60 10801.0 61 9235.5 62 7581.5 63 5570.0 64 3680.0 65 2700.0 66 1939.5 67 1385.0 68 979.0 69 605.5 70 480.0 71 360.5 72 289.5 73 299.0 74 230.5 75 157.0 76 122.0 77 72.5 78 44.0 79 29.0 80 15.5 81 9.5 82 9.0 83 5.0 84 1.5 85 2.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04208626333622536 2 0.0 3 0.0 4 0.0 5 0.0012378312745948633 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006245421430910447 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.813252896806508E-4 22-23 9.002409269780826E-4 24-25 1.687951738083905E-4 26-27 0.0022506023174452064 28-29 0.0041636142872736315 30-31 2.813252896806508E-4 32-33 2.813252896806508E-4 34-35 0.0 36-37 5.626505793613016E-5 38-39 1.1253011587226032E-4 40-41 0.0 42-43 0.010240240544375688 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.1253011587226032E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.063855214251714E-4 84-85 1.687951738083905E-4 86-87 0.0 88-89 0.0 90-91 0.011478071818970552 92-93 0.0016316866801477747 94-95 0.0 96-97 0.0 98-99 3.37590347616781E-4 100-101 0.015191565642755141 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 888651.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.07535621982198 #Duplication Level Percentage of deduplicated Percentage of total 1 76.25392119201517 37.42188345656376 2 12.567281989155502 12.334876806655181 3 4.346562301648113 6.399272798550913 4 2.063626560219794 4.050928341898891 5 1.1172213243828706 2.7414017235235324 6 0.7160930887605208 2.1085514050485092 7 0.47765540012576935 1.6408776238045608 8 0.33504901361706463 1.3154119755485945 9 0.2497944682567288 1.103287726029598 >10 1.6316767938186052 15.332062341569092 >50 0.15913426465748762 5.396041615766203 >100 0.07875952905772275 6.830243355992831 >500 0.0018423281626760587 0.5451898524685403 >1k 9.211640813380293E-4 1.0108163433827997 >5k 4.6058204066901467E-4 1.7691546331970418 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8619 0.9698970687030116 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7035 0.7916493651613513 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4715 0.5305794963377073 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1649 0.18556216107335727 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1306 0.14696433132917197 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1274 0.14336336762125965 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.6265057936130154E-5 0.0 18-19 0.0 0.0 0.0 1.1253011587226031E-4 0.0 20-21 0.0 0.0 0.0 2.813252896806508E-4 0.0 22-23 0.0 0.0 0.0 4.5012046348904123E-4 0.0 24-25 0.0 0.0 0.0 0.001406626448403254 0.0 26-27 0.0 0.0 0.0 0.0022506023174452064 0.0 28-29 0.0 0.0 0.0 0.004726264866634933 0.0 30-31 0.0 0.0 0.0 0.009902650196758907 0.0 32-33 0.0 0.0 0.0 0.015923011395924835 0.0 34-35 0.0 0.0 0.0 0.021999637653026892 0.0 36-37 0.0 0.0 0.0 0.02751361333076765 0.0 38-39 0.0 0.0 0.0 0.04096096217750275 0.0 40-41 0.0 0.0 0.0 0.05975349152817023 0.0 42-43 0.0 0.0 0.0 0.08788602049623531 0.0 44-45 0.0 0.0 0.0 0.11472445313176939 0.0 46-47 0.0 0.0 0.0 0.16783866782347628 0.0 48-49 0.0 0.0 0.0 0.22888625568417748 0.0 50-51 0.0 0.0 0.0 0.2605634833022188 0.0 52-53 0.0 0.0 0.0 0.31277745706694754 0.0 54-55 0.0 0.0 0.0 0.3970625138552705 0.0 56-57 0.0 0.0 0.0 0.5342367251035558 0.0 58-59 0.0 0.0 0.0 0.7028068386802018 0.0 60-61 0.0 0.0 0.0 0.906542613466929 0.0 62-63 0.0 0.0 0.0 1.120518628798032 0.0 64-65 0.0 0.0 0.0 1.3497424748298261 0.0 66-67 0.0 0.0 0.0 1.6436711374881703 0.0 68-69 0.0 0.0 0.0 1.9978034121381734 0.0 70-71 0.0 0.0 0.0 2.235748342150068 0.0 72-73 0.0 0.0 0.0 2.537047727398045 0.0 74-75 0.0 0.0 0.0 2.8746380750148255 0.0 76-77 0.0 0.0 0.0 3.2427240840329894 0.0 78-79 0.0 0.0 0.0 3.6837296081363773 0.0 80-81 0.0 0.0 0.0 4.32008741339401 0.0 82-83 0.0 0.0 0.0 4.948961965946136 0.0 84-85 0.0 0.0 0.0 5.765705546947002 0.0 86-87 0.0 0.0 0.0 6.692053460807449 0.0 88-89 0.0 0.0 0.0 7.857134015490896 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGAACG 25 0.0050215377 56.998177 9 GGTATCA 5130 0.0 39.655136 1 GTATCAA 8255 0.0 35.12253 1 ATCAACG 10570 0.0 27.411612 3 TCAACGC 10665 0.0 27.167439 4 CGTATAG 140 5.7710713E-7 27.160328 1 TATCAAC 10710 0.0 27.09764 2 GTATAGG 495 0.0 26.885979 1 CAACGCA 10790 0.0 26.852709 5 AACGCAG 10950 0.0 26.590475 6 GTACATG 7890 0.0 24.69903 1 GGTACTC 290 0.0 24.568178 8 ACGCAGA 12370 0.0 23.499653 7 TACATGG 8235 0.0 23.417587 2 CTGGTCG 265 3.092282E-11 23.301142 9 CGCAGAG 12415 0.0 23.184921 8 GAGTACT 6440 0.0 23.122318 12-13 ACATGGG 7975 0.0 22.751623 3 AGTACTT 6685 0.0 22.523588 12-13 CGTCTTA 85 3.1514683E-6 22.352226 14-15 >>END_MODULE