FastQCFastQC Report
Thu 26 May 2016
SRR937434_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937434_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887056
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118291.3335122021608556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86060.9701755018848867No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57220.645055103623672No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20170.22738136036507278No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16640.18758680398982702No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12480.14069010299237028No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40650.054.3130041
GTATCAA78550.044.8262021
TATAACG608.2378596E-539.6250952
TATCAAC103550.033.7970852
ATCAACG103700.033.6087383
TCAACGC104350.033.353824
CAACGCA106100.032.80745
AACGCAG109300.032.028196
ATGGGCG1201.4905527E-731.701875
GTACATG65850.028.3977011
ACGCAGA122750.028.3640067
CGCAGAG122800.028.1590588
GCAGAGT126200.026.9863979
TACATGG68600.026.6169742
ACATGGG67850.025.4380473
GTATAGG2451.0913936E-1125.247811
ATGGGAG17900.025.237525
GAGTACT80500.025.2246612-13
AGTACTT83700.024.2886512-13
CATGGGA51400.024.0512264
Produced by FastQC (version 0.11.5)