##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937434_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 887056 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.884058052704678 33.0 31.0 34.0 30.0 34.0 2 31.9665466441803 33.0 31.0 34.0 30.0 34.0 3 31.9730614527155 33.0 31.0 34.0 30.0 34.0 4 35.614399767320215 37.0 35.0 37.0 33.0 37.0 5 35.63503769773273 37.0 35.0 37.0 35.0 37.0 6 35.70541431431612 37.0 35.0 37.0 35.0 37.0 7 35.65981403654335 37.0 35.0 37.0 33.0 37.0 8 35.66787327970275 37.0 35.0 37.0 33.0 37.0 9 37.29320471311845 39.0 37.0 39.0 34.0 39.0 10-11 37.19120100647535 39.0 37.0 39.0 34.0 39.0 12-13 37.07011902292527 39.0 37.0 39.0 33.5 39.0 14-15 38.20054483595173 40.0 38.0 41.0 33.5 41.0 16-17 38.19165419094172 40.0 38.0 41.0 33.5 41.0 18-19 38.281016643819555 40.0 38.0 41.0 33.5 41.0 20-21 38.2935530564023 40.0 38.0 41.0 34.0 41.0 22-23 38.14810000721488 40.0 38.0 41.0 34.0 41.0 24-25 38.00103150195704 40.0 38.0 41.0 33.0 41.0 26-27 37.83463614473044 40.0 37.5 41.0 33.0 41.0 28-29 37.83337748687794 40.0 38.0 41.0 33.0 41.0 30-31 37.56261949640158 40.0 37.0 41.0 33.0 41.0 32-33 37.25350259735575 39.5 36.0 41.0 33.0 41.0 34-35 37.072321251420426 39.0 35.5 41.0 32.0 41.0 36-37 37.00447886041016 40.0 35.5 41.0 32.0 41.0 38-39 36.86969537436194 40.0 35.0 41.0 31.5 41.0 40-41 36.62116540556627 39.5 35.0 41.0 31.0 41.0 42-43 36.260540484478994 39.0 35.0 41.0 30.0 41.0 44-45 35.827917290452916 39.0 35.0 41.0 29.0 41.0 46-47 35.19031605670894 39.0 35.0 41.0 25.0 41.0 48-49 34.653338120704895 39.0 35.0 41.0 21.5 41.0 50-51 33.699710615789755 38.0 34.0 40.0 16.5 40.5 52-53 33.747043591385435 38.0 34.0 40.0 14.5 40.5 54-55 34.04098952039105 39.0 34.5 40.0 12.5 41.0 56-57 33.865235678469006 38.5 34.0 40.0 8.5 41.0 58-59 33.6474563049007 38.0 34.0 40.0 7.5 41.0 60-61 33.37265403762558 38.0 34.0 40.0 4.0 41.0 62-63 33.069455592431595 38.0 33.5 40.0 2.0 41.0 64-65 32.71842363954474 37.0 33.0 40.0 2.0 41.0 66-67 32.349268253639 36.5 33.0 39.5 2.0 41.0 68-69 31.8970634322974 36.0 32.0 39.0 2.0 41.0 70-71 31.474409732869177 35.5 31.5 39.0 2.0 40.5 72-73 31.086629254522826 35.0 31.0 38.5 2.0 40.0 74-75 30.648627595101097 35.0 31.0 37.5 2.0 40.0 76-77 30.231857966126153 35.0 30.5 37.0 2.0 39.0 78-79 29.770716843130536 35.0 30.0 36.5 2.0 39.0 80-81 29.311753147490123 34.5 29.5 36.0 2.0 38.5 82-83 28.859437284681015 34.0 29.0 35.5 2.0 37.0 84-85 28.43426457856099 34.0 29.0 35.0 2.0 37.0 86-87 28.03975679100305 34.0 28.5 35.0 2.0 36.0 88-89 27.669933465305462 34.0 27.0 35.0 2.0 36.0 90-91 27.285696167998413 34.0 26.5 35.0 2.0 36.0 92-93 26.897048777078336 34.0 25.5 35.0 2.0 35.0 94-95 26.453578466297504 33.5 25.0 35.0 2.0 35.0 96-97 25.929388899911615 33.0 23.5 35.0 2.0 35.0 98-99 25.294176466874696 33.0 19.0 35.0 2.0 35.0 100-101 23.546922065799677 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24507256976864156 1101 2 0.22368178742603817 1101 3 0.2732167054785144 1101 4 0.19895633914325828 1101 5 0.21217913055401993 1101 6 0.35181587933298175 1101 7 0.2774552312304408 1101 8 0.2556212025278626 1101 9 0.4708794424692968 1101 10-11 0.3708935430696485 1101 12-13 0.22633625037341432 1101 14-15 0.48391019086634657 1101 16-17 0.5942811822418577 1101 18-19 0.4803588767845497 1101 20-21 0.6070234451003529 1101 22-23 0.5711600271759494 1101 24-25 0.46046648724652073 1101 26-27 0.37728907728986627 1101 28-29 0.47909394864753096 1101 30-31 0.5317656636591153 1101 32-33 0.5308058486889422 1101 34-35 0.7503058899533883 1101 36-37 0.8799035583449992 1101 38-39 0.8401139673328544 1101 40-41 1.038134935980466 1101 42-43 1.1154503358855905 1101 44-45 1.3485562531144595 1101 46-47 1.387071171413183 1101 48-49 1.6565114587702965 1101 50-51 1.5056732994695992 1101 52-53 1.674760491298045 1101 54-55 1.617629936243489 1101 56-57 1.4379813532007475 1101 58-59 1.4804954722280357 1101 60-61 1.4749247849271399 1101 62-63 1.6210647869340065 1101 64-65 1.568963729513591 1101 66-67 1.622609364832769 1101 68-69 1.8448016769484603 1101 70-71 1.6954425244908151 1101 72-73 1.611031956627837 1101 74-75 1.5528077987187707 1101 76-77 1.5888512741048721 1101 78-79 1.530322047481853 1101 80-81 1.5940758200578422 1101 82-83 1.6331114773906243 1101 84-85 1.5129715894112543 1101 86-87 1.5322292881438564 1101 88-89 1.4382513692502599 1101 90-91 1.480141212336271 1101 92-93 1.5993073235998665 1101 94-95 1.7356459138854596 1101 96-97 1.8833142023151588 1101 98-99 1.996658047288502 1101 100-101 2.2224263577085317 1102 1 0.12305965631481541 1102 2 0.26107460664655946 1102 3 0.35237860280383515 1102 4 0.18956376837215316 1102 5 0.2161927716096912 1102 6 0.09339595842271109 1102 7 0.17276870635740238 1102 8 0.16341157038087317 1102 9 0.15046902770667003 1102 10-11 0.2849563711929406 1102 12-13 0.28478722745986573 1102 14-15 0.4623835283996627 1102 16-17 0.5149512857920158 1102 18-19 0.4221445181833161 1102 20-21 0.3395632191888609 1102 22-23 0.32881359996719084 1102 24-25 0.40073008023906453 1102 26-27 0.3133547625813691 1102 28-29 0.32378349040625665 1102 30-31 0.425556498833636 1102 32-33 0.3714542424930798 1102 34-35 0.48212723116611755 1102 36-37 0.6381131845765324 1102 38-39 0.6613711804994935 1102 40-41 0.7056534291939514 1102 42-43 0.8309538748108167 1102 44-45 0.8884187777745467 1102 46-47 0.8842778901950723 1102 48-49 0.9148757743365366 1102 50-51 0.8439744379002789 1102 52-53 0.8548493415392429 1102 54-55 0.9437396029959331 1102 56-57 0.8716131215985783 1102 58-59 0.9560362194100591 1102 60-61 1.1314603353756851 1102 62-63 1.1018531405721603 1102 64-65 0.9817032687582454 1102 66-67 1.1114729158255372 1102 68-69 1.0046049286779954 1102 70-71 1.210814148610524 1102 72-73 1.1301545752846103 1102 74-75 1.2329138038001517 1102 76-77 1.0706567914564005 1102 78-79 1.1702209522340397 1102 80-81 1.0243961127549106 1102 82-83 1.2334723205805957 1102 84-85 1.5238947860086824 1102 86-87 1.4644108810003473 1102 88-89 1.3635127587331617 1102 90-91 1.4054679676757154 1102 92-93 1.4403369689383325 1102 94-95 1.207060922004434 1102 96-97 1.376848010660101 1102 98-99 1.5378681142219541 1102 100-101 2.007192563856467 1103 1 0.11788573600239616 1103 2 0.08193810435800941 1103 3 0.19391317709574096 1103 4 -0.04007224024579159 1103 5 -0.028835034820957617 1103 6 -0.06020026633778741 1103 7 0.03875464384261562 1103 8 0.07684792788040795 1103 9 0.12190300675484878 1103 10-11 0.1591694160838486 1103 12-13 0.20927802121281047 1103 14-15 0.23614485679944153 1103 16-17 0.08465126654990485 1103 18-19 0.01585960242276485 1103 20-21 -0.1359251677227178 1103 22-23 -0.1128752266739852 1103 24-25 -0.0547613349394922 1103 26-27 0.04559129546803575 1103 28-29 -0.06320379402687593 1103 30-31 -0.021631786820258014 1103 32-33 0.2974342496221283 1103 34-35 0.2852176407076783 1103 36-37 0.39417650059196063 1103 38-39 0.2077938824076142 1103 40-41 0.27127376653027113 1103 42-43 0.5186688896524743 1103 44-45 0.6347821761209858 1103 46-47 1.0890190206849937 1103 48-49 1.2499636098511644 1103 50-51 1.1769847560891264 1103 52-53 1.168090438907491 1103 54-55 1.1697356977932998 1103 56-57 1.2707248299512344 1103 58-59 1.1082236691360947 1103 60-61 1.3179312371998932 1103 62-63 1.249405764597043 1103 64-65 1.2259140739933585 1103 66-67 1.3847181770608827 1103 68-69 1.3491775487282744 1103 70-71 1.4285011204381775 1103 72-73 1.3215719619532997 1103 74-75 1.4031191266102567 1103 76-77 1.351802990508176 1103 78-79 1.4318345138229986 1103 80-81 1.3125546425312926 1103 82-83 1.0409571871318093 1103 84-85 1.1298734051820283 1103 86-87 1.3776851000386827 1103 88-89 1.255517503018094 1103 90-91 1.1448452171891432 1103 92-93 1.0928815018123785 1103 94-95 1.1235643461542288 1103 96-97 1.2896821846529676 1103 98-99 1.580536711408822 1103 100-101 1.867698599886534 1104 1 0.09910879819701535 1104 2 0.15171435005206035 1104 3 0.2304668637279086 1104 4 0.10653869292566043 1104 5 0.14035185285951712 1104 6 0.14638736960070986 1104 7 0.12475752774109594 1104 8 0.16956714761147396 1104 9 0.1785017930382864 1104 10-11 0.24833814723535141 1104 12-13 0.2132532559017406 1104 14-15 0.4793162579572581 1104 16-17 0.34893935500404183 1104 18-19 0.3236586458629276 1104 20-21 0.3930927694452322 1104 22-23 0.2734659957562542 1104 24-25 0.2831445512880961 1104 26-27 0.23767436827218802 1104 28-29 0.3371452391136529 1104 30-31 0.32939930107058757 1104 32-33 0.22492814427658203 1104 34-35 0.5621879552689393 1104 36-37 0.6169679879264436 1104 38-39 0.5859999103995364 1104 40-41 0.7204430810240794 1104 42-43 1.0220547055630078 1104 44-45 1.2632782042283637 1104 46-47 1.243087012875499 1104 48-49 1.3606130095858688 1104 50-51 1.4414818755327161 1104 52-53 1.5138984864790572 1104 54-55 1.661773683099156 1104 56-57 1.6344439166073386 1104 58-59 1.6887887090677012 1104 60-61 1.5639825871208117 1104 62-63 1.4891545871266914 1104 64-65 1.590398189764933 1104 66-67 1.6257880268320122 1104 68-69 1.6877755462843105 1104 70-71 1.798789492056386 1104 72-73 1.7902167058872998 1104 74-75 1.7550288738346715 1104 76-77 1.727622176271705 1104 78-79 1.8532659021351279 1104 80-81 1.6826197034267558 1104 82-83 1.589665965035774 1104 84-85 1.5696399710398552 1104 86-87 1.5490555716162397 1104 88-89 1.3547728908829946 1104 90-91 1.4300272898650874 1104 92-93 1.5366397088612231 1104 94-95 1.553387052631578 1104 96-97 1.56829649148737 1104 98-99 1.3531732788283044 1104 100-101 1.6372115516046328 1105 1 0.06733361057853315 1105 2 0.14851804774484378 1105 3 0.18077229781377824 1105 4 0.10298407744281235 1105 5 0.17802556965821026 1105 6 0.10933543154797576 1105 7 0.20209180169817387 1105 8 0.18073870771200262 1105 9 0.1622159012786213 1105 10-11 0.23056135030717684 1105 12-13 0.14547565307888277 1105 14-15 0.16639912996753736 1105 16-17 0.14100860739414145 1105 18-19 0.057780970757505656 1105 20-21 -0.005225638305233815 1105 22-23 0.03812305037235575 1105 24-25 0.13612549373853966 1105 26-27 0.12188156007636763 1105 28-29 0.05974915923336965 1105 30-31 0.03461139395428603 1105 32-33 0.17312864343535495 1105 34-35 0.3252558475567753 1105 36-37 0.18143553330761364 1105 38-39 0.4921726917404783 1105 40-41 0.23378001815313354 1105 42-43 0.27815408849565415 1105 44-45 0.503557254562061 1105 46-47 0.6827434785685611 1105 48-49 0.811213525110503 1105 50-51 0.6176957079090997 1105 52-53 0.5441567323314942 1105 54-55 0.5674409205045663 1105 56-57 0.5958328822973016 1105 58-59 0.7085991502392446 1105 60-61 0.763102426140243 1105 62-63 0.9160087958186978 1105 64-65 0.733980106480054 1105 66-67 0.6205249146155154 1105 68-69 0.6475452835750453 1105 70-71 0.5413919987608118 1105 72-73 0.6593425085274092 1105 74-75 0.8329765576313548 1105 76-77 0.7795128052043161 1105 78-79 0.6888392094153346 1105 80-81 0.8938375714757605 1105 82-83 0.8492328648333114 1105 84-85 0.8515940052866604 1105 86-87 0.8339675493189951 1105 88-89 0.7090565930887927 1105 90-91 0.7741908145756753 1105 92-93 0.9407122046674701 1105 94-95 1.0600753586037541 1105 96-97 1.0330513073280336 1105 98-99 0.9138134443714669 1105 100-101 0.9649949568692335 1106 1 0.00442623862914715 1106 2 -0.11773867157824114 1106 3 -0.029475831656636586 1106 4 -0.08433334662902325 1106 5 0.0678581400703635 1106 6 0.0328858939099419 1106 7 0.06850251574100952 1106 8 -0.03193948563826865 1106 9 0.05747002279088065 1106 10-11 -0.33260444261450317 1106 12-13 -0.3592174833507684 1106 14-15 -0.23154347639687245 1106 16-17 -0.16182374691159396 1106 18-19 -0.1550725257084693 1106 20-21 -0.1364914023623527 1106 22-23 -0.17356528486765654 1106 24-25 -0.29453041193630014 1106 26-27 -0.27323806163160214 1106 28-29 -0.13759643423568235 1106 30-31 -0.06303357492185313 1106 32-33 0.05639679221934557 1106 34-35 0.03668840648355598 1106 36-37 0.08433838666807247 1106 38-39 0.11792774190587352 1106 40-41 -0.027788854058449886 1106 42-43 0.08789542572595366 1106 44-45 0.29777368741568466 1106 46-47 0.28495879613166863 1106 48-49 0.3496086855355216 1106 50-51 0.3486379168190368 1106 52-53 0.1840153715259376 1106 54-55 0.5920615630509332 1106 56-57 0.2913366596260758 1106 58-59 0.3721293771812171 1106 60-61 0.05634675788024168 1106 62-63 0.30955106729371096 1106 64-65 0.5643138926797704 1106 66-67 0.6019745202590627 1106 68-69 0.3256630721564697 1106 70-71 0.4223917856768509 1106 72-73 0.7403756181477696 1106 74-75 0.7131179637739145 1106 76-77 0.8994333596457587 1106 78-79 0.9388441209250402 1106 80-81 0.7309413070944828 1106 82-83 0.6660343083637947 1106 84-85 0.6863965097032398 1106 86-87 0.8986305970845585 1106 88-89 0.7565236897518339 1106 90-91 0.981353760332965 1106 92-93 1.2011582301982529 1106 94-95 1.3349665119924659 1106 96-97 1.3113453813271079 1106 98-99 1.549020883873002 1106 100-101 1.5978898309154523 1107 1 -0.12840102176063084 1107 2 -0.1347068190728855 1107 3 0.10981363292405533 1107 4 -0.08391474645257802 1107 5 -0.03458529773219965 1107 6 -0.03416382432812526 1107 7 -0.012327233733437026 1107 8 0.0362902961779028 1107 9 0.030378710637961603 1107 10-11 0.024125297239301347 1107 12-13 0.18461374197075742 1107 14-15 0.14894668496378216 1107 16-17 0.1602471264992147 1107 18-19 0.07258302673118067 1107 20-21 -0.11602144434519346 1107 22-23 -0.02302325393012694 1107 24-25 -0.03773967313257032 1107 26-27 0.15111095880304504 1107 28-29 0.10125129969426894 1107 30-31 0.18388423820437794 1107 32-33 0.2554329883411981 1107 34-35 0.2116284821795702 1107 36-37 0.32532364252590895 1107 38-39 0.429906666563987 1107 40-41 0.45236243561901546 1107 42-43 0.4583929151483659 1107 44-45 0.5653160028785109 1107 46-47 0.8598626409077568 1107 48-49 0.8972835611324541 1107 50-51 0.7601556648524337 1107 52-53 0.7819396673258225 1107 54-55 0.9599901369650894 1107 56-57 0.8097110580802251 1107 58-59 0.970321310991217 1107 60-61 1.1702530856001658 1107 62-63 1.2168823018264874 1107 64-65 1.2213856769809368 1107 66-67 1.2953365858718229 1107 68-69 1.2738103825735685 1107 70-71 1.3888669265578173 1107 72-73 1.2662029419928338 1107 74-75 1.2054194050276266 1107 76-77 1.1193591374335448 1107 78-79 1.1558591434516643 1107 80-81 1.3363517911354776 1107 82-83 1.3393250584257146 1107 84-85 1.3195471873442592 1107 86-87 1.5559480147410945 1107 88-89 1.1426731412730255 1107 90-91 1.1015549545204593 1107 92-93 1.2943663655749802 1107 94-95 1.4868825545116025 1107 96-97 1.647955702431311 1107 98-99 1.8235437922538118 1107 100-101 2.1041125425848826 1108 1 0.002169395369069349 1108 2 -0.08462948796974246 1108 3 -0.05147613531176276 1108 4 -0.06369753197619588 1108 5 0.005333590532949017 1108 6 0.05329249791917334 1108 7 0.020665078982091245 1108 8 0.040239337845044076 1108 9 -0.055333938439218855 1108 10-11 0.14603978622391622 1108 12-13 0.010237259791281872 1108 14-15 0.1118380662691223 1108 16-17 0.06503993642508732 1108 18-19 0.1301752225908146 1108 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-3.2505779636912884 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3293.0 3 617.0 4 521.0 5 529.0 6 585.0 7 766.0 8 890.0 9 1225.0 10 1554.0 11 2318.0 12 3691.0 13 5552.0 14 6327.0 15 8982.0 16 12550.0 17 18293.0 18 14238.0 19 7070.0 20 6326.0 21 6163.0 22 6375.0 23 6877.0 24 7226.0 25 8107.0 26 9082.0 27 10256.0 28 11997.0 29 14277.0 30 17295.0 31 21611.0 32 27470.0 33 36478.0 34 50758.0 35 75058.0 36 111502.0 37 173165.0 38 170618.0 39 27413.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.272195722418836 19.90181747418155 15.507476263316528 20.31851054008309 2 14.801756336073982 21.220547277577314 41.650788553284315 22.326907833064393 3 17.694496080905665 28.25219247660529 31.682134990524517 22.371176451964523 4 11.166418649671849 17.545807758936526 39.74430880151253 31.5434647898791 5 11.45199500189408 38.81481565243941 34.65078279158916 15.082406554077357 6 27.365196936864063 38.769706679200006 18.732558586203236 15.1325377977327 7 25.286085135321372 35.94189506408533 22.03154900556468 16.740470795028614 8 26.04263751111352 33.53611626070541 20.76350314002867 19.657743088152394 9 27.292105575292712 15.806697580895685 21.798033312811707 35.10316353099989 10-11 23.34546070843455 28.018699508502344 27.782667645895714 20.85317213716739 12-13 25.354047561081007 26.311957264054435 28.771352862459914 19.562642312404645 14-15 22.406447287337063 26.07416662383312 29.291710041521245 22.227676047308577 16-17 19.078973752988777 29.919909533188772 29.59653135243251 21.404585361389945 18-19 19.025093646497464 26.611815499455517 35.0079752023171 19.355115651729918 20-21 20.36709465572804 25.44270153834337 35.11018261544353 19.080021190485066 22-23 19.78188061606583 25.769715179685864 35.09666269435612 19.351741509892186 24-25 19.242026796176816 26.10173511598029 35.32859905973373 19.32763902810916 26-27 19.232483027869815 26.12157811302555 35.29429371597738 19.351645143127254 28-29 18.784992250303887 25.988538131668015 35.671591376373144 19.554878241654954 30-31 18.938280364666365 25.94265170611118 35.58039189544935 19.538676033773104 32-33 18.780640594964872 26.179516393336133 35.19843553972919 19.841407471969806 34-35 19.11795278544199 26.206915642449264 35.11274865377271 19.562382918336034 36-37 19.413286585720556 25.93560644353492 34.75014443905406 19.900962531690464 38-39 18.99958829632048 26.486947750906285 34.68064162521863 19.83282232755461 40-41 19.0322287348461 26.422660693299843 34.8688605782073 19.676249993646756 42-43 19.14474353036858 26.527465576251853 34.17987326186899 20.147917631510577 44-45 19.193997597276688 26.784660300447726 33.834512573065886 20.186829529209692 46-47 19.573932076537456 27.122671750335382 33.00255595565911 20.30084021746805 48-49 19.645699759920916 27.533455726592287 32.169806524502185 20.65103798898461 50-51 19.8403668771156 27.745646780221715 31.708681131453005 20.705305211209684 52-53 19.90051606654789 28.122999345098947 31.322634544306965 20.653850044046195 54-55 19.72925842635697 28.00702048129952 31.365700531394168 20.898020560949334 56-57 19.83245050315673 28.309427270981807 30.96407314125659 20.89404908460487 58-59 19.920681396834144 27.613501668423844 31.439158859135784 21.026658075606225 60-61 20.093885684889464 27.75590851312655 31.649346623170327 20.50085917881366 62-63 20.00454058230144 27.54394029924922 31.958583564221566 20.492935554227767 64-65 19.86090539115841 27.49382934257017 31.95900797399712 20.686257292274295 66-67 19.797359907799493 27.763375369793042 31.488720853475844 20.950543868931625 68-69 19.961490139612362 27.664309208204518 31.516368102316523 20.857832549866597 70-71 19.91348420889159 27.774053733534753 31.551608758876764 20.760853298696897 72-73 19.983080930865047 27.71265592678493 31.245782233057355 21.05848090929267 74-75 19.588102211464285 28.06404537912511 31.116259158900355 21.231593250510254 76-77 19.623569224756597 27.885821495274847 31.399597092787822 21.091012187180727 78-79 19.646321372921875 27.705352902074775 31.155504174462784 21.492821550540565 80-81 19.466066818737076 27.986960875284 31.010503927607104 21.536468378371815 82-83 19.705798809484147 27.767503242831467 30.952282800880266 21.574415146804125 84-85 19.511382552700347 27.384418359349418 30.89924312066477 22.204955967285468 86-87 19.537111177115648 27.29363574454437 31.080871997750325 22.08838108058966 88-89 19.641914300444814 26.921593507339086 31.207192433511153 22.229299758704947 90-91 19.15613825444836 27.11626168139854 31.537799503491147 22.18980056066195 92-93 19.259794249883956 27.176001165518276 31.398237944170322 22.16596664042745 94-95 18.79081581082752 26.96360577848827 31.63650614118254 22.609072269501667 96-97 18.963568130047513 27.203795537014052 31.400931266312128 22.43170506662631 98-99 18.928034309769462 27.475544857911405 30.94882167470214 22.647599157616998 100-101 18.73838580554161 27.406703892318525 31.28739057741921 22.567519724720654 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1663.0 1 1008.0 2 485.5 3 638.0 4 513.5 5 460.0 6 911.5 7 2009.0 8 3491.5 9 4135.5 10 3426.0 11 2777.5 12 2746.5 13 2841.0 14 3002.5 15 3089.5 16 3160.0 17 3315.5 18 3462.5 19 3594.0 20 3857.0 21 4257.0 22 4763.5 23 5300.0 24 5849.5 25 6743.0 26 8247.5 27 9911.5 28 10877.5 29 12295.5 30 14472.0 31 16287.0 32 18660.0 33 21923.0 34 25507.0 35 27735.5 36 30173.5 37 32809.5 38 33054.5 39 32180.0 40 30960.0 41 30541.0 42 29980.0 43 30065.5 44 31042.5 45 31030.0 46 29743.5 47 28491.5 48 28932.0 49 27944.0 50 24907.5 51 22021.5 52 19997.5 53 19316.5 54 18789.0 55 17387.0 56 15519.0 57 13513.0 58 12240.5 59 11040.0 60 9509.0 61 8294.5 62 6938.5 63 5162.5 64 3546.5 65 2578.0 66 1825.0 67 1322.0 68 932.0 69 574.0 70 467.0 71 377.5 72 275.0 73 249.5 74 209.0 75 158.5 76 114.5 77 72.0 78 46.0 79 29.5 80 19.5 81 15.5 82 11.0 83 7.5 84 7.0 85 4.0 86 2.5 87 5.5 88 5.0 89 2.0 90 1.0 91 1.5 92 2.5 93 2.5 94 2.5 95 4.0 96 4.0 97 4.5 98 4.5 99 3.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35984199419202395 2 0.30606861348099784 3 0.3007701881279198 4 0.30527948630075213 5 0.3067450082069227 6 0.21982828592557857 7 0.20630039140708142 8 0.2111478869428762 9 0.20415847477498603 10-11 0.22614130336754387 12-13 0.21413529698237765 14-15 0.20816047690337477 16-17 0.21075332335275337 18-19 0.2042712072293068 20-21 0.19761999242437905 22-23 0.19852185205894557 24-25 0.18809410003427066 26-27 0.19249066575278223 28-29 0.2096823650367057 30-31 0.22242113237495717 32-33 0.2135716347107736 34-35 0.22433758409841092 36-37 0.1955344420194441 38-39 0.19282886311574465 40-41 0.1895596219404412 42-43 0.1990855143305496 44-45 0.2041021085478256 46-47 0.21064059089843257 48-49 0.21768636929348317 50-51 0.22140654028606988 52-53 0.2468277087354124 54-55 0.21774273552064355 56-57 0.18555761981205246 58-59 0.18482485885896718 60-61 0.18234474486390936 62-63 0.19243429952562183 64-65 0.18211927995526778 66-67 0.17975189841453076 68-69 0.17682085460218971 70-71 0.18809410003427066 72-73 0.18764317021698743 74-75 0.19333615916018831 76-77 0.19621083674536896 78-79 0.19530897711080247 80-81 0.19395618765895276 82-83 0.1850503237676088 84-85 0.1778918129182374 86-87 0.17980826464169117 88-89 0.18324660449847585 90-91 0.1887141285330351 92-93 0.18183744881946573 94-95 0.1803155606861348 96-97 0.17980826464169117 98-99 0.19282886311574465 100-101 0.1753553326960192 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 887056.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.7064242823268 #Duplication Level Percentage of deduplicated Percentage of total 1 77.74225261674246 39.42031645848374 2 12.026581388712982 12.196498771240309 3 4.038807891197563 6.1438051957761965 4 1.8228988955240852 3.6973073928091673 5 1.030850574898391 2.6135373311239154 6 0.6296680471690502 1.9156929094066817 7 0.4270709957767315 1.515867017737152 8 0.315995552030698 1.2818403626077317 9 0.21633741225742928 0.987272695265927 >10 1.5341127622587947 14.980008480016874 >50 0.14258078936909316 4.96553627275911 >100 0.06928975338227655 5.99581303684692 >500 0.002220825425312163 0.7859060014682719 >1k 6.662476275936489E-4 0.5550552631089142 >5k 4.4416508506243263E-4 1.613477745957501 >10k+ 2.2208254253121631E-4 1.3320650653916304 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11829 1.3335122021608556 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8606 0.9701755018848867 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5722 0.645055103623672 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2017 0.22738136036507278 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1664 0.18758680398982702 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1248 0.14069010299237028 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.636622716040475E-5 0.0 14-15 0.0 0.0 0.0 1.127324543208095E-4 0.0 16-17 0.0 0.0 0.0 1.127324543208095E-4 0.0 18-19 0.0 0.0 0.0 1.127324543208095E-4 0.0 20-21 0.0 0.0 0.0 1.127324543208095E-4 0.0 22-23 0.0 0.0 0.0 5.636622716040476E-4 0.0 24-25 0.0 0.0 0.0 0.001578254360491333 0.0 26-27 0.0 0.0 0.0 0.002536480222218214 0.0 28-29 0.0 0.0 0.0 0.004678396854313595 0.0 30-31 0.0 0.0 0.0 0.00924406125430638 0.0 32-33 0.0 0.0 0.0 0.01448612038022402 0.0 34-35 0.0 0.0 0.0 0.020122743096264495 0.0 36-37 0.0 0.0 0.0 0.025759365812304973 0.0 38-39 0.0 0.0 0.0 0.037934470878952395 0.0 40-41 0.0 0.0 0.0 0.053886113165346944 0.0 42-43 0.0 0.0 0.0 0.07727809743691492 0.0 44-45 0.0 0.0 0.0 0.09762630544182103 0.0 46-47 0.0 0.0 0.0 0.14170469508125755 0.0 48-49 0.0 0.0 0.0 0.2004383037823993 0.0 50-51 0.0 0.0 0.0 0.22924144586136613 0.0 52-53 0.0 0.0 0.0 0.27997105030573044 0.0 54-55 0.0 0.0 0.0 0.3524580184340109 0.0 56-57 0.0 0.0 0.0 0.4692488411103696 0.0 58-59 0.0 0.0 0.0 0.6136027488681661 0.0 60-61 0.0 0.0 0.0 0.7958347612777548 0.0 62-63 0.0 0.0 0.0 0.9857889468083187 0.0 64-65 0.0 0.0 0.0 1.1840853339586226 0.0 66-67 0.0 0.0 0.0 1.4513739831532622 0.0 68-69 0.0 0.0 0.0 1.7741833661009 0.0 70-71 0.0 0.0 0.0 1.9907987770783355 0.0 72-73 0.0 0.0 0.0 2.27544822423838 0.0 74-75 0.0 0.0 0.0 2.5905918002922026 0.0 76-77 0.0 0.0 0.0 2.933242095200303 0.0 78-79 0.0 0.0 0.0 3.3474774986021174 0.0 80-81 0.0 0.0 0.0 3.954429032665356 0.0 82-83 0.0 0.0 0.0 4.5543911545607045 0.0 84-85 0.0 0.0 0.0 5.349267689976732 0.0 86-87 0.0 0.0 0.0 6.286525315199942 0.0 88-89 0.0 0.0 0.0 7.454208077054886 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4065 0.0 54.313004 1 GTATCAA 7855 0.0 44.826202 1 TATAACG 60 8.2378596E-5 39.625095 2 TATCAAC 10355 0.0 33.797085 2 ATCAACG 10370 0.0 33.608738 3 TCAACGC 10435 0.0 33.35382 4 CAACGCA 10610 0.0 32.8074 5 AACGCAG 10930 0.0 32.02819 6 ATGGGCG 120 1.4905527E-7 31.70187 5 GTACATG 6585 0.0 28.397701 1 ACGCAGA 12275 0.0 28.364006 7 CGCAGAG 12280 0.0 28.159058 8 GCAGAGT 12620 0.0 26.986397 9 TACATGG 6860 0.0 26.616974 2 ACATGGG 6785 0.0 25.438047 3 GTATAGG 245 1.0913936E-11 25.24781 1 ATGGGAG 1790 0.0 25.23752 5 GAGTACT 8050 0.0 25.22466 12-13 AGTACTT 8370 0.0 24.28865 12-13 CATGGGA 5140 0.0 24.051226 4 >>END_MODULE