FastQCFastQC Report
Thu 26 May 2016
SRR937434_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937434_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887056
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87720.9888890893021409No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71850.8099826842950164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48190.543257697371981No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17580.1981836546959831No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13970.15748723868617087No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13080.14745405025161884No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC250.005019024557.005383
GGTATCA51700.040.7256661
GTATCAA86150.037.294641
ATCAACG109100.029.3474083
TATCAAC110600.028.9923362
TCAACGC110600.028.9064334
CAACGCA112450.028.4308745
AACGCAG115050.027.7836676
GTATAGG5000.027.5665861
TAAGGTG3200.025.2367575
ACGCAGA129600.024.5586457
GAGTACT68350.024.46463412-13
CGCAGAG130100.024.464268
GTACATG80150.024.372121
GCAGAGT134150.023.5132149
CATATAG2853.6379788E-1223.3355353
TACATGG82750.023.3073352
AGTACTT70400.023.111212-13
GTACTTT72850.022.59096714-15
ACATGGG82600.022.0268973
Produced by FastQC (version 0.11.5)