FastQCFastQC Report
Thu 26 May 2016
SRR937433_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937433_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977971
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122941.2570924904726213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92710.9479831201538697No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62420.6382602347104362No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20900.21370776843076125No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17360.17751037607454617No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15780.16135447779126375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45750.051.861721
GTATCAA86350.043.4462281
TATCAAC110350.033.9849052
ATCAACG110600.033.7791563
TCAACGC111550.033.4932024
CAACGCA112900.033.0506025
AACGCAG114850.032.5091446
ACGCAGA130450.028.4044787
GTACATG72600.028.3588561
CGCAGAG130300.028.2913448
TACATGG74050.027.4084322
GCAGAGT135350.026.8132299
GTACCGG908.893343E-426.415871
ACATGGG75350.025.8001043
ATGGGAT21400.025.321815
GAGTACT85650.024.92965712-13
CATGGGA56500.024.8185984
GTACTTT91150.023.423614-15
AGTACTT87300.023.34302112-13
CGTCACT1903.2536627E-722.5230051
Produced by FastQC (version 0.11.5)