##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937433_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 977971 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82500912603748 33.0 31.0 34.0 30.0 34.0 2 31.89719429308231 33.0 31.0 34.0 30.0 34.0 3 31.90868440884239 33.0 31.0 34.0 30.0 34.0 4 35.56235000833358 37.0 35.0 37.0 33.0 37.0 5 35.56902914299095 37.0 35.0 37.0 33.0 37.0 6 35.65530368487409 37.0 35.0 37.0 33.0 37.0 7 35.60984834928643 37.0 35.0 37.0 33.0 37.0 8 35.611776831828344 37.0 35.0 37.0 33.0 37.0 9 37.23041992042709 39.0 37.0 39.0 34.0 39.0 10-11 37.14810255109814 39.0 37.0 39.0 34.0 39.0 12-13 37.05162780900456 39.0 37.0 39.0 33.0 39.0 14-15 38.16804537148852 40.0 38.0 41.0 33.0 41.0 16-17 38.14711734806042 40.0 38.0 41.0 33.0 41.0 18-19 38.207102766850966 40.0 38.0 41.0 33.5 41.0 20-21 38.20516150274395 40.0 38.0 41.0 34.0 41.0 22-23 38.07238813829858 40.0 38.0 41.0 33.5 41.0 24-25 37.94361182489051 40.0 38.0 41.0 33.0 41.0 26-27 37.76638724461155 40.0 37.0 41.0 33.0 41.0 28-29 37.577060055973035 40.0 37.0 41.0 33.0 41.0 30-31 37.42844317469536 39.0 37.0 41.0 33.0 41.0 32-33 37.16198537584448 39.0 36.0 41.0 32.0 41.0 34-35 36.92077934826288 39.0 35.0 41.0 31.5 41.0 36-37 36.849988394338894 39.0 35.0 41.0 31.0 41.0 38-39 36.77204743289934 39.0 35.0 41.0 31.0 41.0 40-41 36.53939687373143 39.5 35.0 41.0 30.5 41.0 42-43 36.191478070413126 39.0 35.0 41.0 30.0 41.0 44-45 35.76252414437647 39.0 35.0 41.0 28.5 41.0 46-47 35.16431673331827 39.0 35.0 41.0 25.0 41.0 48-49 34.652059723652336 39.0 35.0 41.0 21.5 41.0 50-51 33.669708508739006 38.0 34.0 40.0 17.0 40.5 52-53 33.75402389232401 38.0 34.0 40.0 16.0 40.5 54-55 34.094164857649154 39.0 35.0 40.0 14.0 41.0 56-57 33.89431179452151 38.5 34.0 40.0 10.5 41.0 58-59 33.67022028260552 38.0 34.0 40.0 8.0 41.0 60-61 33.4091184707931 38.0 34.0 40.0 6.5 41.0 62-63 33.12056185715118 38.0 33.5 40.0 2.0 41.0 64-65 32.7785420017567 37.0 33.0 40.0 2.0 41.0 66-67 32.404742574166306 36.5 32.5 39.5 2.0 41.0 68-69 31.93610751239045 36.0 32.0 39.0 2.0 41.0 70-71 31.522682165422083 35.5 31.5 39.0 2.0 40.5 72-73 31.12524911270375 35.0 31.0 38.5 2.0 40.0 74-75 30.687805671129308 35.0 31.0 37.5 2.0 40.0 76-77 30.26797215868364 35.0 30.5 37.0 2.0 39.0 78-79 29.81265037511337 35.0 30.0 36.5 2.0 39.0 80-81 29.37224825685015 34.5 29.5 36.0 2.0 38.5 82-83 28.956743093609116 34.0 29.0 35.5 2.0 37.0 84-85 28.50828347670841 34.0 29.0 35.0 2.0 37.0 86-87 28.118796978642514 34.0 28.5 35.0 2.0 36.0 88-89 27.758633435960782 34.0 27.5 35.0 2.0 36.0 90-91 27.362869144381584 34.0 26.5 35.0 2.0 36.0 92-93 26.989866775190677 34.0 26.0 35.0 2.0 35.0 94-95 26.543157721445727 33.5 25.0 35.0 2.0 35.0 96-97 26.01749745135592 33.0 24.5 35.0 2.0 35.0 98-99 25.413403362676398 33.0 20.0 35.0 2.0 35.0 100-101 23.74531504512915 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18526304948381167 1101 2 0.15936451745895397 1101 3 0.320627944199412 1101 4 0.16908264302572462 1101 5 0.19325766391030896 1101 6 0.19844626443661184 1101 7 0.15012371860562723 1101 8 0.11843480584040122 1101 9 0.19036837533316486 1101 10-11 0.13178798143283643 1101 12-13 0.1871662242202845 1101 14-15 0.26697966203511214 1101 16-17 0.2873506162750843 1101 18-19 0.15790312572740106 1101 20-21 0.16694008528944693 1101 22-23 0.14196003038747307 1101 24-25 0.3084180676224477 1101 26-27 0.12266183391709973 1101 28-29 0.29745782168825485 1101 30-31 0.2561396186252267 1101 32-33 0.2387727824654533 1101 34-35 0.13080068333427164 1101 36-37 0.3169024172294357 1101 38-39 0.349107152498199 1101 40-41 0.42115716986478446 1101 42-43 0.44125709815763514 1101 44-45 0.5224631861919278 1101 46-47 0.6461455068311466 1101 48-49 0.8092367062296475 1101 50-51 0.8983188609793444 1101 52-53 0.9528530641005446 1101 54-55 0.9937656230296668 1101 56-57 1.0738814844173632 1101 58-59 0.9494949280064375 1101 60-61 1.0107395208506489 1101 62-63 1.1103678332297662 1101 64-65 1.0051131312853485 1101 66-67 1.1233832550342697 1101 68-69 1.0801578610835207 1101 70-71 1.053867215416485 1101 72-73 1.23107894828901 1101 74-75 1.24350393333739 1101 76-77 1.2404023381685363 1101 78-79 1.236250539959169 1101 80-81 1.3305897634014592 1101 82-83 1.2521470140644695 1101 84-85 1.2879203549628109 1101 86-87 1.4866490607251528 1101 88-89 1.4542659536711788 1101 90-91 1.395753281424387 1101 92-93 1.4165041024271972 1101 94-95 1.246132901406991 1101 96-97 1.2121784286114803 1101 98-99 1.2885594890780965 1101 100-101 1.7754657105068716 1102 1 0.11281431584775348 1102 2 -0.017751355217431097 1102 3 0.02838966418271127 1102 4 -0.0683573827365862 1102 5 0.03688500862232047 1102 6 0.10176631407949799 1102 7 0.0012450240640689003 1102 8 0.0805983547924285 1102 9 0.23794910927767177 1102 10-11 0.09606314733346721 1102 12-13 0.23211616667702373 1102 14-15 0.2929061376086892 1102 16-17 0.4222821633182221 1102 18-19 0.39163399980781577 1102 20-21 0.438778273547328 1102 22-23 0.3885805460507399 1102 24-25 0.3461279380494844 1102 26-27 0.27746556848189385 1102 28-29 0.23325525777595857 1102 30-31 0.3988990373631296 1102 32-33 0.4430688123442934 1102 34-35 0.6837327221503813 1102 36-37 0.6681294490302676 1102 38-39 0.8539283707081538 1102 40-41 0.9543484516123968 1102 42-43 1.1383455498826436 1102 44-45 1.1799733696271275 1102 46-47 1.3858099081411837 1102 48-49 1.5830509586255914 1102 50-51 1.528388693713481 1102 52-53 1.5345574600369645 1102 54-55 1.6648512143236331 1102 56-57 1.607348721618287 1102 58-59 1.768791149453655 1102 60-61 1.8791900043061673 1102 62-63 1.8986969059854957 1102 64-65 1.8066291403390125 1102 66-67 1.8286206373470364 1102 68-69 1.8010728110308207 1102 70-71 1.8310019054418838 1102 72-73 1.7965989691238917 1102 74-75 1.6033925229916335 1102 76-77 1.7580549999747 1102 78-79 1.9155197381412563 1102 80-81 2.0160711116742114 1102 82-83 1.8827137121095525 1102 84-85 1.9284284815841737 1102 86-87 1.721376817818399 1102 88-89 2.0307846665063103 1102 90-91 2.0400285152226694 1102 92-93 1.3607669206549993 1102 94-95 1.2733559796246006 1102 96-97 1.4333952806144872 1102 98-99 1.5352464474427698 1102 100-101 1.714683733259374 1103 1 0.10824747469634843 1103 2 0.10742067998578975 1103 3 0.11619380392883016 1103 4 -0.06257583191830918 1103 5 0.12369684347630994 1103 6 0.09118870405969659 1103 7 0.2330058858849995 1103 8 0.2122658747407229 1103 9 0.2775337525443078 1103 10-11 0.23614068240723896 1103 12-13 0.05943126898322504 1103 14-15 0.22771817880758505 1103 16-17 0.20337016552961273 1103 18-19 0.20115891011591458 1103 20-21 0.21202395247075145 1103 22-23 0.1567481448383674 1103 24-25 0.15854873372324363 1103 26-27 0.1571394753891937 1103 28-29 0.07804057146888965 1103 30-31 0.02969153808826519 1103 32-33 0.0389023971199407 1103 34-35 0.20917835902616844 1103 36-37 0.4336315971586586 1103 38-39 0.636697791949608 1103 40-41 0.6762753237544672 1103 42-43 0.558087599276945 1103 44-45 0.6361214259943324 1103 46-47 0.8645748754471967 1103 48-49 1.1201835527509942 1103 50-51 0.9648527108595815 1103 52-53 0.8678015719070586 1103 54-55 0.9532870906957811 1103 56-57 1.0325465012691737 1103 58-59 1.1095549273564487 1103 60-61 1.2033009205359804 1103 62-63 1.1049598147090123 1103 64-65 1.2394857715052723 1103 66-67 1.3163771814980265 1103 68-69 1.2997218358564524 1103 70-71 1.2785452816728942 1103 72-73 1.3867276524292294 1103 74-75 1.295246084290671 1103 76-77 1.2719146620751758 1103 78-79 1.086865827223857 1103 80-81 1.0106638043390426 1103 82-83 1.0904429350262959 1103 84-85 1.025806976551106 1103 86-87 1.1757495292878666 1103 88-89 1.235787119285444 1103 90-91 1.3582111518823758 1103 92-93 1.190700510686593 1103 94-95 1.2067841959285737 1103 96-97 1.569038590075401 1103 98-99 1.5043243971530131 1103 100-101 1.6863312611110715 1104 1 0.09267856380487416 1104 2 0.17888853407026417 1104 3 0.2268043047281516 1104 4 0.0407871224246108 1104 5 0.15723070015495466 1104 6 0.14135224141522684 1104 7 0.09554027029057721 1104 8 0.10878693645771165 1104 9 0.35224819041847866 1104 10-11 0.3169191472824622 1104 12-13 0.2537135506966095 1104 14-15 0.3749912030694986 1104 16-17 0.37461030447837373 1104 18-19 0.2693311288693465 1104 20-21 0.22285322797706897 1104 22-23 0.31588390983943526 1104 24-25 0.27211924132695486 1104 26-27 0.38240662506454015 1104 28-29 0.34199150639609854 1104 30-31 0.381142207218943 1104 32-33 0.392254236335269 1104 34-35 0.39281573137134274 1104 36-37 0.38785998500122076 1104 38-39 0.6713673247083065 1104 40-41 0.5100964339914 1104 42-43 0.7434555486615437 1104 44-45 0.7791917238604142 1104 46-47 0.7610920710328486 1104 48-49 0.9506346046286751 1104 50-51 0.890949435666414 1104 52-53 0.8404274289800426 1104 54-55 0.9926189362128994 1104 56-57 0.9043066832093203 1104 58-59 0.9045579042775813 1104 60-61 0.9976116183146928 1104 62-63 1.0886725282886118 1104 64-65 0.9354614187439907 1104 66-67 0.897065275553274 1104 68-69 0.8214427282368888 1104 70-71 0.883589761513921 1104 72-73 0.9887352861055483 1104 74-75 1.0604544784350303 1104 76-77 1.0074031381041983 1104 78-79 1.0537823748458983 1104 80-81 0.8852904810318769 1104 82-83 1.008460895139418 1104 84-85 1.0827050730613443 1104 86-87 1.0015795876857787 1104 88-89 1.0836120821222543 1104 90-91 1.3428749337707089 1104 92-93 1.2056225694788516 1104 94-95 1.3130397414107087 1104 96-97 1.4806218578455912 1104 98-99 1.4032883706081556 1104 100-101 1.265296510389554 1105 1 0.21081052545546441 1105 2 0.18077962291435412 1105 3 0.21957002368231926 1105 4 0.21682372602661815 1105 5 0.13486162963940984 1105 6 0.1070080985021562 1105 7 0.1776910905526421 1105 8 0.2118339541176013 1105 9 0.3204917573123254 1105 10-11 0.36293351493785053 1105 12-13 0.3711850597502533 1105 14-15 0.27329183695810144 1105 16-17 0.4073682062025412 1105 18-19 0.41049322150413303 1105 20-21 0.27572621197059277 1105 22-23 0.3052118104208077 1105 24-25 0.4456468545065917 1105 26-27 0.2833761196313773 1105 28-29 0.32317362232452496 1105 30-31 0.4561745459931288 1105 32-33 0.46787317129542316 1105 34-35 0.5137077344544707 1105 36-37 0.5860030585464031 1105 38-39 0.5771639284075647 1105 40-41 0.4474768352353564 1105 42-43 0.3908522773392349 1105 44-45 0.5018833918846681 1105 46-47 0.4673152790878774 1105 48-49 0.6346166991600128 1105 50-51 0.729510262955479 1105 52-53 0.6932501419843646 1105 54-55 0.7748194195924683 1105 56-57 0.8376441298077921 1105 58-59 1.0538709484747173 1105 60-61 1.006411472911779 1105 62-63 0.9982488781892869 1105 64-65 1.0651587472694573 1105 66-67 1.170099661639341 1105 68-69 1.115987870004723 1105 70-71 1.035395687533505 1105 72-73 1.2178865026999617 1105 74-75 1.1115205638142562 1105 76-77 1.1866815214572632 1105 78-79 1.1704545326202123 1105 80-81 1.2839175420208555 1105 82-83 1.3708390472580945 1105 84-85 1.3117866883466398 1105 86-87 1.186454645737527 1105 88-89 1.246772139602605 1105 90-91 1.1907948576165417 1105 92-93 1.2382503866602264 1105 94-95 1.401763782249958 1105 96-97 1.548960313511074 1105 98-99 1.7720826084707788 1105 100-101 1.8215887726691697 1106 1 -0.04878957951151364 1106 2 0.03103766673807229 1106 3 -0.2948592912623518 1106 4 -0.09489539983170658 1106 5 0.010665283073429066 1106 6 0.05957614480426088 1106 7 -0.036036986656405645 1106 8 0.009980266423518458 1106 9 -0.05119340006525164 1106 10-11 -0.12043942399176899 1106 12-13 -0.21858539787661613 1106 14-15 -0.012191011145937125 1106 16-17 -0.19350943437302703 1106 18-19 -0.07631301236812504 1106 20-21 -0.14595858093831282 1106 22-23 -0.22916123056005233 1106 24-25 -0.28274926463092953 1106 26-27 -0.15759817803747467 1106 28-29 -0.027518139225492178 1106 30-31 0.24796452456237006 1106 32-33 0.09418525853465098 1106 34-35 0.06562083054299706 1106 36-37 -0.1301542023985931 1106 38-39 -0.08876408173923522 1106 40-41 -0.028513790630263713 1106 42-43 -0.11968451212908349 1106 44-45 0.12662461912359646 1106 46-47 0.22389029403109362 1106 48-49 0.14031881162046744 1106 50-51 0.2602258607569965 1106 52-53 0.4504520430529837 1106 54-55 0.35666450582576914 1106 56-57 0.4596463749348416 1106 58-59 0.39466920777231707 1106 60-61 0.4231704716434308 1106 62-63 0.696889774490657 1106 64-65 0.6240477738465131 1106 66-67 0.6755448236821096 1106 68-69 0.6316928253208616 1106 70-71 0.808264274707124 1106 72-73 0.7996076695828762 1106 74-75 0.6742201104152201 1106 76-77 0.6485344073202519 1106 78-79 0.6742668749875058 1106 80-81 0.9551230586187636 1106 82-83 0.77184570974552 1106 84-85 0.7842765925642752 1106 86-87 0.596086459479455 1106 88-89 0.7288821945644806 1106 90-91 0.9254725944118434 1106 92-93 0.9013847443935212 1106 94-95 1.0141185414819525 1106 96-97 0.8986625426015067 1106 98-99 0.9030598947827961 1106 100-101 1.1672854909931978 1107 1 -0.003922374356342573 1107 2 -0.10663489943560123 1107 3 0.09302583616252491 1107 4 0.04023202504467349 1107 5 0.01985576032937786 1107 6 -0.017281528656909018 1107 7 -0.1624494002334771 1107 8 -0.11206345406962015 1107 9 0.04294326563184114 1107 10-11 0.054487298313731 1107 12-13 0.11873252663057343 1107 14-15 0.11959976145828222 1107 16-17 0.039835272851810544 1107 18-19 0.012022968657987576 1107 20-21 0.17425935028134631 1107 22-23 0.037997496757419924 1107 24-25 0.15886351589397663 1107 26-27 0.054289030250167514 1107 28-29 0.11622920375887702 1107 30-31 0.1256019621924267 1107 32-33 0.09035076543729303 1107 34-35 0.1004081574174478 1107 36-37 -0.04341522761507122 1107 38-39 0.03196791208529959 1107 40-41 0.1791305832090444 1107 42-43 0.2826939363975356 1107 44-45 0.21320417296872307 1107 46-47 0.06748716602447047 1107 48-49 0.011263366696212529 1107 50-51 -0.16291366892325954 1107 52-53 -0.031334813949221996 1107 54-55 0.13423314073502723 1107 56-57 0.16316258552711105 1107 58-59 0.14863235636381233 1107 60-61 0.23095916338486688 1107 62-63 0.27153513282417663 1107 64-65 0.25933741651986253 1107 66-67 0.29288702492167573 1107 68-69 0.3851888985314318 1107 70-71 0.30333900676807346 1107 72-73 0.4983160802959645 1107 74-75 0.38085202145274266 1107 76-77 0.32346672334290005 1107 78-79 0.4127801768468622 1107 80-81 0.5940570080068106 1107 82-83 0.6260818317333019 1107 84-85 0.743884361118635 1107 86-87 0.7256369483703331 1107 88-89 0.5299640194587596 1107 90-91 0.8079737690924453 1107 92-93 1.0482338035303016 1107 94-95 0.907365246782561 1107 96-97 1.0859905357995459 1107 98-99 1.1859481546892674 1107 100-101 1.174713947351016 1108 1 -0.09309909980113318 1108 2 -0.40622317427642685 1108 3 -0.27346777704432057 1108 4 -0.31266630916015714 1108 5 -0.3409134670597922 1108 6 -0.2911413751278076 1108 7 -0.2690728145203849 1108 8 -0.2652695934761553 1108 9 -0.22654227565272578 1108 10-11 -0.24208389234972572 1108 12-13 -0.07392364990592881 1108 14-15 0.03798854073932034 1108 16-17 -0.1293499331645549 1108 18-19 -0.12122032268260341 1108 20-21 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-1.4099963735100545 2316 92-93 -1.7324034513716597 2316 94-95 -1.6704533806684196 2316 96-97 -1.7516877173198822 2316 98-99 -1.819232326594154 2316 100-101 -1.6878083342889134 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3898.0 3 565.0 4 489.0 5 530.0 6 670.0 7 797.0 8 1122.0 9 1409.0 10 1814.0 11 2663.0 12 4133.0 13 5983.0 14 7121.0 15 9736.0 16 13507.0 17 19468.0 18 15224.0 19 7782.0 20 6756.0 21 6804.0 22 6919.0 23 7472.0 24 8099.0 25 8787.0 26 10146.0 27 11586.0 28 13425.0 29 16159.0 30 19817.0 31 24238.0 32 31242.0 33 41110.0 34 57124.0 35 83502.0 36 123280.0 37 189174.0 38 185098.0 39 30318.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.233579488779334 19.800359586721562 15.51107699507005 20.454983929429055 2 14.823461830416676 21.261617052705002 41.51683318630368 22.398087930574647 3 17.704299514479946 28.194322913407554 31.739963132071097 22.3614144400414 4 11.176979267430756 17.600372572814287 39.67295246516618 31.54969569458877 5 11.49559684178785 38.77015165408182 34.761462262893055 14.972789241237274 6 27.505372883770455 38.6343395170605 18.74682930678782 15.113458292381216 7 25.338389791421108 35.78557884487265 22.016604315072 16.85942704863424 8 26.094540952338 33.61993598330606 20.720049442288122 19.565473622067824 9 27.284234174678872 15.813073499101671 21.757222258093943 35.14547006812552 10-11 23.41945687536259 28.000981945728537 27.732535613470084 20.84702556543879 12-13 25.310158564209793 26.29126820618472 28.787296413599417 19.611276816006068 14-15 22.429036904345278 26.099846639677025 29.123806820307102 22.347309635670594 16-17 19.1472679119041 29.92813099591663 29.488071713212744 21.43652937896653 18-19 19.112000864930895 26.70228187011587 34.700433131567365 19.48528413338587 20-21 20.400837708998097 25.5479722656216 34.95154785030897 19.09964217507134 22-23 19.89255714306943 25.826971293060026 34.84672944414924 19.43374211972131 24-25 19.35469491650476 26.129921792380905 35.05662674307076 19.458756548043578 26-27 19.262601494782096 26.28787238240185 35.00403545047157 19.44549067234448 28-29 18.960797376242695 26.0517577124116 35.4105770216255 19.5768678897202 30-31 19.04020991564425 26.081045068314836 35.256347180796 19.622397835244918 32-33 18.883327661633203 26.33829914596496 34.91708726081484 19.861285931587 34-35 19.262232917370415 26.277917616384894 34.779108522047125 19.680740944197563 36-37 19.49104384536907 26.094725099830242 34.488612460883914 19.925618593916774 38-39 19.176437737125116 26.542232287186213 34.4278127745912 19.853517201097468 40-41 19.142464090011295 26.502250538617805 34.631190902546685 19.724094468824212 42-43 19.18759577761303 26.57672174269016 33.94135733048739 20.294325149209417 44-45 19.248578728742423 26.846269028431575 33.648436883462416 20.256715359363582 46-47 19.681719685203984 27.132684604990846 32.76561490418439 20.419980805620785 48-49 19.721604716208585 27.60338997990159 31.987785167351817 20.68722013653801 50-51 19.90103703551853 27.816229208680653 31.527622249498393 20.755111506302416 52-53 19.951973962404377 28.14038774490332 31.19980523058392 20.70783306210838 54-55 19.80186703595997 28.015892459384737 31.256656453697733 20.925584050957553 56-57 19.895282057062058 28.267389377584006 30.856187016819426 20.981141548534513 58-59 19.968312396942295 27.71251465666897 31.26612617772002 21.053046768668715 60-61 20.11596359001471 27.836179656474002 31.444391165537567 20.60346558797372 62-63 20.037387467462707 27.693775227447258 31.707728160631888 20.561109144458143 64-65 19.95589229728899 27.576586545357678 31.740999159853285 20.726521997500054 66-67 19.895665111856182 27.791844622787675 31.28473730807381 21.027752957282335 68-69 19.992500026895396 27.73427554306926 31.41612709380653 20.857097336228815 70-71 19.947732335455736 27.84567257346266 31.3838071680783 20.822787923003304 72-73 20.00819838030749 27.793636519685077 31.031740541759216 21.166424558248213 74-75 19.672339442279245 28.12171303238949 30.929426996209212 21.276520529122052 76-77 19.63080000676378 27.974078319466727 31.203579065276127 21.191542608493364 78-79 19.713871557320225 27.802295606620277 30.98643654237639 21.49739629368311 80-81 19.540656227345966 28.053270762302663 30.796371768237858 21.609701242113513 82-83 19.857394362588874 27.85416226873723 30.775958714458753 21.512484654215147 84-85 19.568244269178045 27.438378423790876 30.694552876015074 22.29882443101601 86-87 19.609158068383252 27.37712224761007 30.908839411047474 22.10488027295921 88-89 19.730910227409552 26.996641501410263 31.082123103959745 22.19032516722044 90-91 19.233131898956625 27.179039211818623 31.34865395896682 22.23917493025794 92-93 19.244721349776864 27.2397821419971 31.306481941662014 22.209014566564022 94-95 18.909178661291737 26.912603232848838 31.506929326454813 22.671288779404613 96-97 19.025008286912108 27.15950269305431 31.261357680545814 22.554131339487764 98-99 18.96896250660961 27.37653865545185 30.922978074544087 22.73152076339445 100-101 18.78032325337292 27.370650241202892 31.28834670105031 22.560679804373873 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1978.0 1 1218.0 2 563.5 3 657.0 4 542.5 5 527.0 6 970.5 7 2152.5 8 3688.5 9 4321.0 10 3685.5 11 3028.5 12 2944.0 13 3013.5 14 3143.5 15 3287.0 16 3449.5 17 3548.5 18 3597.0 19 3785.0 20 4177.0 21 4660.5 22 5236.0 23 5762.0 24 6334.5 25 7361.0 26 8951.5 27 10696.5 28 11808.5 29 13454.0 30 15917.0 31 17897.5 32 20504.0 33 23929.5 34 27791.0 35 30440.0 36 33170.5 37 36086.0 38 36368.5 39 35657.0 40 34359.5 41 33596.5 42 33035.0 43 33133.5 44 34025.5 45 34155.5 46 33037.0 47 31526.0 48 31904.5 49 31048.0 50 27784.0 51 24850.0 52 22475.5 53 21264.0 54 20620.5 55 19361.0 56 17382.0 57 14986.0 58 13602.0 59 12344.5 60 10819.0 61 9351.5 62 7773.0 63 5828.0 64 3913.5 65 2865.0 66 2083.0 67 1494.5 68 1056.0 69 698.5 70 512.0 71 407.0 72 324.5 73 278.0 74 236.0 75 182.5 76 131.5 77 79.5 78 43.5 79 27.5 80 20.5 81 13.0 82 4.5 83 4.0 84 2.5 85 3.0 86 3.0 87 2.5 88 3.0 89 2.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 1.5 96 1.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.359826620625765 2 0.3181075921474154 3 0.32107291525004317 4 0.31984588500067995 5 0.30563278461222265 6 0.228841141506241 7 0.2373281007310033 8 0.23395376754525443 9 0.2323177272127701 10-11 0.24100919147909292 12-13 0.23937315114660865 14-15 0.22132558122889123 16-17 0.2358965654400795 18-19 0.22193909635357284 20-21 0.2264382072679047 22-23 0.22459766189385985 24-25 0.21544606128402582 26-27 0.22940353037053246 28-29 0.23221547469198986 30-31 0.23937315114660865 32-33 0.2420317166868956 34-35 0.22935240411014235 36-37 0.22388189424839794 38-39 0.21355438964959084 40-41 0.2140656522534922 42-43 0.21242961192100787 44-45 0.2310906969634069 46-47 0.22132558122889123 48-49 0.2323177272127701 50-51 0.240651307656362 52-53 0.25138782233829016 54-55 0.24683758516356827 56-57 0.20614108189302136 58-59 0.19218361280651472 60-61 0.20194872854103035 62-63 0.2019998548014205 64-65 0.19949466804230392 66-67 0.21217398061905723 68-69 0.20118183463517836 70-71 0.20787937474628593 72-73 0.2217345913120123 74-75 0.20987329890150117 76-77 0.22347288416527689 78-79 0.2198940459379675 80-81 0.20588545059107072 82-83 0.21810462682431275 84-85 0.19765412266825907 86-87 0.20460729408131734 88-89 0.21355438964959084 90-91 0.20997555142228144 92-93 0.21534380876324552 94-95 0.21294087452490923 96-97 0.20803275352745632 98-99 0.216673091533389 100-101 0.19688722876240708 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 977971.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.38436357035917 #Duplication Level Percentage of deduplicated Percentage of total 1 76.68367791247299 37.869746299418864 2 12.527512852157274 12.373264986465639 3 4.2775760906989735 6.3373611858886125 4 1.9338297701122737 3.820038098016345 5 1.0694350247390974 2.640668403829582 6 0.6672967164983694 1.9772414194117423 7 0.4498023956807826 1.5549243530182824 8 0.33108717523804715 1.3080423548351152 9 0.2529858891204409 1.124419241384498 >10 1.5737893783324617 15.023277962500748 >50 0.15119933735238475 5.1235398454960235 >100 0.0780795228593788 6.605261646460042 >500 0.00248528982507831 0.8454358464755736 >1k 6.213224562695775E-4 0.5527141682015484 >5k 4.14214970846385E-4 1.5866496838102557 >10k+ 2.071074854231925E-4 1.2574145047871643 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12294 1.2570924904726213 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9271 0.9479831201538697 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6242 0.6382602347104362 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2090 0.21370776843076125 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1736 0.17751037607454617 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1578 0.16135447779126375 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0225252078026854E-4 0.0 12-13 0.0 0.0 0.0 2.0450504156053708E-4 0.0 14-15 0.0 0.0 0.0 2.0450504156053708E-4 0.0 16-17 0.0 0.0 0.0 4.0901008312107417E-4 0.0 18-19 0.0 0.0 0.0 4.0901008312107417E-4 0.0 20-21 0.0 0.0 0.0 4.601363435112084E-4 0.0 22-23 0.0 0.0 0.0 7.668939058520141E-4 0.0 24-25 0.0 0.0 0.0 0.0010225252078026853 0.0 26-27 0.0 0.0 0.0 0.0017382928532645651 0.0 28-29 0.0 0.0 0.0 0.004652489695502218 0.0 30-31 0.0 0.0 0.0 0.009253853130614303 0.0 32-33 0.0 0.0 0.0 0.014417605430017865 0.0 34-35 0.0 0.0 0.0 0.019530231469031288 0.0 36-37 0.0 0.0 0.0 0.027301423048331698 0.0 38-39 0.0 0.0 0.0 0.038907084156892174 0.0 40-41 0.0 0.0 0.0 0.05536974000251541 0.0 42-43 0.0 0.0 0.0 0.08093287019758255 0.0 44-45 0.0 0.0 0.0 0.10613811656991874 0.0 46-47 0.0 0.0 0.0 0.1535321599515732 0.0 48-49 0.0 0.0 0.0 0.2157016925859765 0.0 50-51 0.0 0.0 0.0 0.2470932164655189 0.0 52-53 0.0 0.0 0.0 0.2982194768556532 0.0 54-55 0.0 0.0 0.0 0.3673421809031147 0.0 56-57 0.0 0.0 0.0 0.4845746959776926 0.0 58-59 0.0 0.0 0.0 0.6388737498351178 0.0 60-61 0.0 0.0 0.0 0.828194292059785 0.0 62-63 0.0 0.0 0.0 1.0207357886890307 0.0 64-65 0.0 0.0 0.0 1.224882946426837 0.0 66-67 0.0 0.0 0.0 1.4794917231697053 0.0 68-69 0.0 0.0 0.0 1.7971903052339997 0.0 70-71 0.0 0.0 0.0 2.0218902196486397 0.0 72-73 0.0 0.0 0.0 2.301806495284625 0.0 74-75 0.0 0.0 0.0 2.6156194815592695 0.0 76-77 0.0 0.0 0.0 2.954484335425079 0.0 78-79 0.0 0.0 0.0 3.3601712116207945 0.0 80-81 0.0 0.0 0.0 3.9455668930878316 0.0 82-83 0.0 0.0 0.0 4.534694791563349 0.0 84-85 0.0 0.0 0.0 5.309257636473883 0.0 86-87 0.0 0.0 0.0 6.235409843441165 0.0 88-89 0.0 0.0 0.0 7.357733511525392 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4575 0.0 51.86172 1 GTATCAA 8635 0.0 43.446228 1 TATCAAC 11035 0.0 33.984905 2 ATCAACG 11060 0.0 33.779156 3 TCAACGC 11155 0.0 33.493202 4 CAACGCA 11290 0.0 33.050602 5 AACGCAG 11485 0.0 32.509144 6 ACGCAGA 13045 0.0 28.404478 7 GTACATG 7260 0.0 28.358856 1 CGCAGAG 13030 0.0 28.291344 8 TACATGG 7405 0.0 27.408432 2 GCAGAGT 13535 0.0 26.813229 9 GTACCGG 90 8.893343E-4 26.41587 1 ACATGGG 7535 0.0 25.800104 3 ATGGGAT 2140 0.0 25.32181 5 GAGTACT 8565 0.0 24.929657 12-13 CATGGGA 5650 0.0 24.818598 4 GTACTTT 9115 0.0 23.4236 14-15 AGTACTT 8730 0.0 23.343021 12-13 CGTCACT 190 3.2536627E-7 22.523005 1 >>END_MODULE